BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0053.p (298 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAT5.1 B3KAT5_9BACI Formyltetrahydrofolate deformylase 523 e-146 B1DJI6.1 B1DJI6_9BACL Formyltetrahydrofolate deformylase 458 e-127 C0Z530.1 C0Z530_BREBN Formyltetrahydrofolate deformylase EC=3.5.... 400 e-110 C5DAV6.1 C5DAV6_9BACI Formyltetrahydrofolate deformylase 394 e-108 Q5KZ65.1 Q5KZ65_GEOKA Formyltetrahydrofolate hydrolase EC=3.5.1.10 393 e-108 >B3KAT5.1 B3KAT5_9BACI Formyltetrahydrofolate deformylase Length = 312 Score = 523 bits (1346), Expect = e-146, Method: Compositional matrix adjust. Identities = 249/299 (83%), Positives = 276/299 (92%), Gaps = 1/299 (0%) Query: 1 MELHVKRE-HSLSGQYPNRARMLISCPDGPGIVAAVSQFLYQHGANIVQSDQYTMDPDGG 59 ME+H+K++ S +GQYPNRARMLISCPDGPGIVAAVSQFLYQHGANIVQSDQYTMDP GG Sbjct: 14 MEIHIKKDLPSAAGQYPNRARMLISCPDGPGIVAAVSQFLYQHGANIVQSDQYTMDPAGG 73 Query: 60 MFFMRVEFDLPKLAERIDEVRSVFSGVAERFKMNWQIFNVSQKKRLAIFVSKEDHCLVEL 119 MFFMRVEFDL L ++ ++ F VA++F+M W I++ ++KKRLAIFVSKEDHCLVEL Sbjct: 74 MFFMRVEFDLEDLNTKLPQLEESFREVADQFRMEWNIYHAARKKRLAIFVSKEDHCLVEL 133 Query: 120 LWQWQAGDLDADIALVVSNHTDMQAYVESFGIPFYHIPVTADTKAEAEQHQLKVIGDDID 179 LWQWQAGDLDADIALVVSNH DM+ YVESFGIP++HIPVTADTK +AEQ QL+VIGDDID Sbjct: 134 LWQWQAGDLDADIALVVSNHLDMKEYVESFGIPYHHIPVTADTKPQAEQRQLEVIGDDID 193 Query: 180 VIILARYMQIISPSFIEHYRHRIINIHHSFLPAFVGGKPYAQAYQRGVKIIGATAHYVTE 239 VIILARYMQIISP+FI+HYR+RIINIHHSFLPAFVGGKPYAQAY RGVKIIGATAHYVTE Sbjct: 194 VIILARYMQIISPTFIDHYRNRIINIHHSFLPAFVGGKPYAQAYNRGVKIIGATAHYVTE 253 Query: 240 ELDGGPIIEQDVQRVSHSDDVNELKRIGRTIERVVLARAVKWHIEDRILVHHNKTVVFN 298 ELDGGPIIEQDVQRVSH DDVNELKRIGRTIERVVLARAVKWH+EDRILVH+NKTVVF+ Sbjct: 254 ELDGGPIIEQDVQRVSHRDDVNELKRIGRTIERVVLARAVKWHVEDRILVHNNKTVVFS 312 >B1DJI6.1 B1DJI6_9BACL Formyltetrahydrofolate deformylase Length = 278 Score = 458 bits (1178), Expect = e-127, Method: Compositional matrix adjust. Identities = 217/278 (78%), Positives = 244/278 (87%) Query: 21 MLISCPDGPGIVAAVSQFLYQHGANIVQSDQYTMDPDGGMFFMRVEFDLPKLAERIDEVR 80 MLISCPD PGIVAAVS FLY+HGANIVQSDQYTMDP+GGMFF+R EFDL + + + + Sbjct: 1 MLISCPDRPGIVAAVSHFLYEHGANIVQSDQYTMDPEGGMFFIRFEFDLNDMDKELPVLV 60 Query: 81 SVFSGVAERFKMNWQIFNVSQKKRLAIFVSKEDHCLVELLWQWQAGDLDADIALVVSNHT 140 F+ VA+RF M W F S+KKRLAIFVSKEDHCL+ELLWQW+AGDLDADIA+VVSNH Sbjct: 61 EDFARVADRFDMKWHTFRASRKKRLAIFVSKEDHCLMELLWQWKAGDLDADIAMVVSNHP 120 Query: 141 DMQAYVESFGIPFYHIPVTADTKAEAEQHQLKVIGDDIDVIILARYMQIISPSFIEHYRH 200 DM+ VESFGIP++HIPVTADTKAEAE+ Q++++ D D+I+LARYMQIISP FIE + + Sbjct: 121 DMKDMVESFGIPYHHIPVTADTKAEAERKQMEIVADKADLIVLARYMQIISPKFIEQFPN 180 Query: 201 RIINIHHSFLPAFVGGKPYAQAYQRGVKIIGATAHYVTEELDGGPIIEQDVQRVSHSDDV 260 RIINIHHSFLPAFVGGKPYAQAY RGVKIIGATAHYVTEELDGGPIIEQDVQRVSH D+V Sbjct: 181 RIINIHHSFLPAFVGGKPYAQAYNRGVKIIGATAHYVTEELDGGPIIEQDVQRVSHRDNV 240 Query: 261 NELKRIGRTIERVVLARAVKWHIEDRILVHHNKTVVFN 298 ELKRIGRTIERVVLARAVKWH EDRI+VH NKTVVFN Sbjct: 241 EELKRIGRTIERVVLARAVKWHTEDRIIVHQNKTVVFN 278 >C0Z530.1 C0Z530_BREBN Formyltetrahydrofolate deformylase EC=3.5.1.10 Length = 298 Score = 400 bits (1028), Expect = e-110, Method: Compositional matrix adjust. Identities = 195/298 (65%), Positives = 239/298 (80%), Gaps = 2/298 (0%) Query: 1 MELHVKREH-SLSGQYPNRARMLISCPDGPGIVAAVSQFLYQHGANIVQSDQYTMDPDGG 59 M+L +RE + + +RARMLISCPD GIVAAVS FL+Q GANIVQSDQYT DP+ G Sbjct: 1 MQLLSEREWLAYREKNKDRARMLISCPDRAGIVAAVSNFLFQQGANIVQSDQYTTDPETG 60 Query: 60 MFFMRVEFDLPKLAERIDEVRSVFSGVAERFKMNWQIFNVSQKKRLAIFVSKEDHCLVEL 119 FFMR+EFDL L ER +E++ FS VAE F M W + +++K++A+FVSKEDHCL+EL Sbjct: 61 RFFMRIEFDLINLDERCEEIKQAFSPVAESFGMEWSLVEANKRKKVALFVSKEDHCLLEL 120 Query: 120 LWQWQAGDLDADIALVVSNHTDMQAYVESFGIPFYHIPVTADTKAEAEQHQLKVIGDDID 179 LW+W++G+L ADIA+VVSNH DMQ VESFGIP+ IPVT D K +AE+ Q+ +D Sbjct: 121 LWRWKSGELFADIAVVVSNHPDMQETVESFGIPYRCIPVTKDNKPQAEEEQIAAAE-GVD 179 Query: 180 VIILARYMQIISPSFIEHYRHRIINIHHSFLPAFVGGKPYAQAYQRGVKIIGATAHYVTE 239 +I+LARYMQI+SP F+E Y RIINIHHSFLPAFVG KPY QAY+RGVK+IGATAHYVTE Sbjct: 180 LIVLARYMQILSPRFLEDYAMRIINIHHSFLPAFVGAKPYEQAYRRGVKLIGATAHYVTE 239 Query: 240 ELDGGPIIEQDVQRVSHSDDVNELKRIGRTIERVVLARAVKWHIEDRILVHHNKTVVF 297 ELD GPIIEQDVQRVSH +DV LK++GR +ER VLARAV+WH+EDR+LV+ NKT+VF Sbjct: 240 ELDAGPIIEQDVQRVSHQEDVETLKQLGRQVERTVLARAVRWHLEDRVLVYGNKTIVF 297 >C5DAV6.1 C5DAV6_9BACI Formyltetrahydrofolate deformylase Length = 300 Score = 394 bits (1012), Expect = e-108, Method: Compositional matrix adjust. Identities = 181/299 (60%), Positives = 239/299 (79%), Gaps = 4/299 (1%) Query: 3 LHVKREH---SLSGQYPNRARMLISCPDGPGIVAAVSQFLYQHGANIVQSDQYTMDPDGG 59 +H R+H S Y NRAR+LISCPD PGIVA+V+ FLY+ GANIV+S QY+ DP+GG Sbjct: 1 MHTFRKHRWQSFLQNYENRARLLISCPDKPGIVASVTSFLYEQGANIVESSQYSTDPEGG 60 Query: 60 MFFMRVEFDLPKLAERIDEVRSVFSGVAERFKMNWQIFNVSQKKRLAIFVSKEDHCLVEL 119 FF+R+EFD P +A R E+ S F +AE F M+W++ + KR+AIFVSK +HCL+EL Sbjct: 61 TFFLRIEFDCPNIALRKQEIESAFQPIAESFHMDWRLRLHNDVKRIAIFVSKAEHCLLEL 120 Query: 120 LWQWQAGDLDADIALVVSNHTDMQAYVESFGIPFYHIPVTADTKAEAEQHQLKVIGD-DI 178 LWQWQAG+L ADIALV+SNH +++ VES GIP++HIPVT +TKAEAEQ Q++++ ++ Sbjct: 121 LWQWQAGELIADIALVISNHEHLRSTVESVGIPYFHIPVTKETKAEAEQKQIELLKKYEV 180 Query: 179 DVIILARYMQIISPSFIEHYRHRIINIHHSFLPAFVGGKPYAQAYQRGVKIIGATAHYVT 238 D I+LARYMQI+SP+F+ + RIINIHHSFLPAF+G +PY +AY+RGVK+IGAT+HYVT Sbjct: 181 DTIVLARYMQILSPAFVAEFPGRIINIHHSFLPAFIGARPYERAYERGVKLIGATSHYVT 240 Query: 239 EELDGGPIIEQDVQRVSHSDDVNELKRIGRTIERVVLARAVKWHIEDRILVHHNKTVVF 297 ++LD GPIIEQDV RV H ++LKR+GR IE+ VLARA+KWH+EDR+++H NKT+VF Sbjct: 241 DDLDEGPIIEQDVARVDHRHHPDDLKRMGRIIEKTVLARALKWHLEDRVIIHGNKTIVF 299 >Q5KZ65.1 Q5KZ65_GEOKA Formyltetrahydrofolate hydrolase EC=3.5.1.10 Length = 300 Score = 393 bits (1010), Expect = e-108, Method: Compositional matrix adjust. Identities = 180/292 (61%), Positives = 235/292 (80%), Gaps = 1/292 (0%) Query: 7 REHSLSGQYPNRARMLISCPDGPGIVAAVSQFLYQHGANIVQSDQYTMDPDGGMFFMRVE 66 R S Y RAR+LISCPD PGIVAAV+ FLY+ GANIV+S QY+ DP+GG FF+R+E Sbjct: 8 RWQSFLQGYEQRARILISCPDRPGIVAAVTSFLYEQGANIVESSQYSTDPEGGTFFLRLE 67 Query: 67 FDLPKLAERIDEVRSVFSGVAERFKMNWQIFNVSQKKRLAIFVSKEDHCLVELLWQWQAG 126 FD P +AER +E+ + F+ +A F+M WQ+ + +R+AIFVSK +HCL+ELLWQWQAG Sbjct: 68 FDCPNIAERKEEIEAAFAPIAAEFEMRWQLRLHNDIRRIAIFVSKAEHCLLELLWQWQAG 127 Query: 127 DLDADIALVVSNHTDMQAYVESFGIPFYHIPVTADTKAEAEQHQLKVIGD-DIDVIILAR 185 +L ADIALV+SNH D++ VESFGIP+ HIPVT +TKA+AE Q++++ D ID I+LAR Sbjct: 128 ELIADIALVISNHPDLRETVESFGIPYVHIPVTKETKADAEAEQIRLLRDYQIDTIVLAR 187 Query: 186 YMQIISPSFIEHYRHRIINIHHSFLPAFVGGKPYAQAYQRGVKIIGATAHYVTEELDGGP 245 YMQI+SP+F+ + RIINIHHSFLPAF+G +PY +AY+RGVK+IGAT+HYVT++LD GP Sbjct: 188 YMQILSPAFVAEFSGRIINIHHSFLPAFIGARPYERAYERGVKLIGATSHYVTDDLDEGP 247 Query: 246 IIEQDVQRVSHSDDVNELKRIGRTIERVVLARAVKWHIEDRILVHHNKTVVF 297 IIEQDV RV H ++LKRIGR IE+ VLARA++WH+EDR+++H NKT+VF Sbjct: 248 IIEQDVARVDHRHHPDDLKRIGRLIEKTVLARALRWHLEDRVIIHGNKTIVF 299 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,794,430,554 Number of extensions: 75061455 Number of successful extensions: 169481 Number of sequences better than 1.0e-05: 2555 Number of HSP's gapped: 167097 Number of HSP's successfully gapped: 2555 Length of query: 298 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 163 Effective length of database: 1,788,859,178 Effective search space: 291584046014 Effective search space used: 291584046014 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)