BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0036.p (299 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A7GCQ9.1 A7GCQ9_CLOBL Hydrolase, alpha/beta fold family 303 2e-80 B1IJA1.1 B1IJA1_CLOBK Hydrolase, alpha/beta fold family 302 2e-80 C3F5F2.1 C3F5F2_BACTU Acetoin dehydrogenase E2 component, probable 276 1e-72 B3YU20.1 B3YU20_BACCE Hydrolase, alpha/beta fold family 276 1e-72 Q6HF64.1 Q6HF64_BACHK Hydrolase, alpha/beta fold family EC=3.7.1.- 274 9e-72 >A7GCQ9.1 A7GCQ9_CLOBL Hydrolase, alpha/beta fold family Length = 271 Score = 303 bits (775), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 136/264 (51%), Positives = 195/264 (73%), Gaps = 2/264 (0%) Query: 34 ITFNYEEAGEGKPMLILHGNGPDHRMMMSCMEPLFSSQDQYRRIYVDLPGMGMSPAAEWI 93 I+ NYE G GKP+++LHG DHR+M CMEP+F+ + Y+RIY+DLPGMG S +AEWI Sbjct: 8 ISINYEIIGNGKPIIMLHGYSVDHRLMRGCMEPIFNDKSDYKRIYIDLPGMGKSESAEWI 67 Query: 94 RSSDDMLRAVKMMIEALIPNERFLLVGQSYGGYLARGLLGEYAELIDGLFLLCPCVIAES 153 +SD ML + I+ +IPNE FLL G+SYGGYL+RG++ + A ++DG+ L+CP +IA++ Sbjct: 68 SNSDTMLDIIIEFIKRIIPNENFLLAGESYGGYLSRGIIYKMANMVDGVVLICPVIIADN 127 Query: 154 SKRELPPHQVMVQDAELLKELSAADREEFISIAVVQDRTVWERFSREILCGLQLADEAFM 213 KR +P H V+V+D +LL +L++ D E F S+ VVQ + ++E++ EI+ G+++A++ F+ Sbjct: 128 KKRNVPNHVVLVKDNKLLSKLTSEDAEAFNSMTVVQSKKIYEKYKNEIISGVKMANKNFL 187 Query: 214 ERIKADGGYPFSFEADAI-APFEKPSLIITGRQDSMTGYKDVWKLLDFLPHATFAVLDRA 272 E + + GY FSF+ D I F KP+L++ G+QDS GYKD W +L+ P ATFAVLDRA Sbjct: 188 EFLMKN-GYEFSFDVDNINKKFNKPALVLLGKQDSCVGYKDAWNILNNFPRATFAVLDRA 246 Query: 273 GHNLHLEQEELFKAMAKEWLSRVS 296 GHNL +EQEELF ++ KEWL RVS Sbjct: 247 GHNLQIEQEELFNSLIKEWLVRVS 270 >B1IJA1.1 B1IJA1_CLOBK Hydrolase, alpha/beta fold family Length = 271 Score = 302 bits (774), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 136/264 (51%), Positives = 195/264 (73%), Gaps = 2/264 (0%) Query: 34 ITFNYEEAGEGKPMLILHGNGPDHRMMMSCMEPLFSSQDQYRRIYVDLPGMGMSPAAEWI 93 I+ NYE G GKP+++LHG DHR+M CMEP+F+ + Y+RIY+DLPGMG S +AEWI Sbjct: 8 ISINYEIIGNGKPIIMLHGYSVDHRLMRGCMEPIFNDKSDYKRIYIDLPGMGKSESAEWI 67 Query: 94 RSSDDMLRAVKMMIEALIPNERFLLVGQSYGGYLARGLLGEYAELIDGLFLLCPCVIAES 153 +SD ML + I+ +IPNE FLL G+SYGGYL+RG++ + A ++DG+ L+CP +IA++ Sbjct: 68 SNSDTMLDIIIEFIKRIIPNENFLLAGESYGGYLSRGIIYKMANMVDGVVLICPVIIADN 127 Query: 154 SKRELPPHQVMVQDAELLKELSAADREEFISIAVVQDRTVWERFSREILCGLQLADEAFM 213 KR +P H V+V+D +LL +L++ D E F S+ VVQ + ++E++ EI+ G+++A++ F+ Sbjct: 128 KKRNVPDHVVLVKDNKLLSKLTSEDAEAFNSMTVVQSKKIYEKYKNEIISGVKMANKNFL 187 Query: 214 ERIKADGGYPFSFEADAI-APFEKPSLIITGRQDSMTGYKDVWKLLDFLPHATFAVLDRA 272 E + + GY FSF+ D I F KP+L++ G+QDS GYKD W +L+ P ATFAVLDRA Sbjct: 188 EFLMKN-GYEFSFDVDNINKKFNKPALVLLGKQDSCVGYKDAWNILNNFPRATFAVLDRA 246 Query: 273 GHNLHLEQEELFKAMAKEWLSRVS 296 GHNL +EQEELF ++ KEWL RVS Sbjct: 247 GHNLQIEQEELFNSLIKEWLVRVS 270 >C3F5F2.1 C3F5F2_BACTU Acetoin dehydrogenase E2 component, probable Length = 271 Score = 276 bits (707), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 127/262 (48%), Positives = 178/262 (67%), Gaps = 3/262 (1%) Query: 38 YEEAGEGKPMLILHGNGPDHRMMMSCMEPLFSSQDQYRRIYVDLPGMGMSPAAEWIRSSD 97 YE GEGKP+LI+HG PDHR+MM CME +F + Y+RIY+DLPGMG S A +WI SSD Sbjct: 12 YEVVGEGKPVLIIHGCAPDHRLMMKCMESVFQKDEGYKRIYIDLPGMGKSNAPDWINSSD 71 Query: 98 DMLRAVKMMIEALIPNERFLLVGQSYGGYLARGLLGEYAELIDGLFLLCPCVIAESSKRE 157 D+L + M IE +IP + FLLVG+S+GGYLARG+L + E ++GL L+CP V+A +R Sbjct: 72 DILEVLTMFIEEIIPKKDFLLVGESFGGYLARGILSKMFERVNGLLLICPVVVAMQKERR 131 Query: 158 LPPHQVMVQDAELLKELSAADREEFISIAVVQDRTVWERFSREILCGLQLADEAFMERIK 217 LP Q++VQD E L L++ +REEF +AVV + ++RF EI GL +A+ F+ER++ Sbjct: 132 LPDKQIIVQDKEFLNTLTSTEREEFSELAVVANEYTYKRFQEEIKPGLDVANYEFIERLQ 191 Query: 218 ADGGYPFSFEADAIAPFEKPSLIITGRQDSMTGYKDVWKLLDFLPHATFAVLDRAGHNLH 277 + F +EKP L++ GRQD GY+D+ +++D P AT AVLD AGHNL Sbjct: 192 KNYSLTIDFHRKK---YEKPVLLLAGRQDISVGYQDIIEIIDGYPRATLAVLDMAGHNLQ 248 Query: 278 LEQEELFKAMAKEWLSRVSNET 299 +EQ ELF+++ EW++R + Sbjct: 249 IEQPELFESLVGEWITRTKQQN 270 >B3YU20.1 B3YU20_BACCE Hydrolase, alpha/beta fold family Length = 271 Score = 276 bits (707), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 127/262 (48%), Positives = 178/262 (67%), Gaps = 3/262 (1%) Query: 38 YEEAGEGKPMLILHGNGPDHRMMMSCMEPLFSSQDQYRRIYVDLPGMGMSPAAEWIRSSD 97 YE GEGKP+LI+HG PDHR+MM CME +F + Y+RIY+DLPGMG S A +WI SSD Sbjct: 12 YEVVGEGKPVLIIHGCAPDHRLMMKCMESVFQKDEGYKRIYIDLPGMGKSNAPDWINSSD 71 Query: 98 DMLRAVKMMIEALIPNERFLLVGQSYGGYLARGLLGEYAELIDGLFLLCPCVIAESSKRE 157 D+L + M IE +IP + FLLVG+S+GGYLARG+L + E ++GL L+CP V+A +R Sbjct: 72 DILEVLTMFIEEIIPKKDFLLVGESFGGYLARGILSKMFERVNGLLLICPVVVAMQKERR 131 Query: 158 LPPHQVMVQDAELLKELSAADREEFISIAVVQDRTVWERFSREILCGLQLADEAFMERIK 217 LP Q++VQD E L L++ +REEF +AVV + ++RF EI GL +A+ F+ER++ Sbjct: 132 LPDKQIIVQDKEFLNTLTSTEREEFSELAVVANEYTYKRFQEEIKPGLDVANYEFIERLQ 191 Query: 218 ADGGYPFSFEADAIAPFEKPSLIITGRQDSMTGYKDVWKLLDFLPHATFAVLDRAGHNLH 277 + F +EKP L++ GRQD GY+D+ +++D P AT AVLD AGHNL Sbjct: 192 KNYSLTIDFHRKK---YEKPVLLLAGRQDISVGYQDIIEIIDGYPRATLAVLDMAGHNLQ 248 Query: 278 LEQEELFKAMAKEWLSRVSNET 299 +EQ ELF+++ EW++R + Sbjct: 249 IEQPELFESLVGEWITRTKQQN 270 >Q6HF64.1 Q6HF64_BACHK Hydrolase, alpha/beta fold family EC=3.7.1.- Length = 271 Score = 274 bits (700), Expect = 9e-72, Method: Compositional matrix adjust. Identities = 126/262 (48%), Positives = 177/262 (67%), Gaps = 3/262 (1%) Query: 38 YEEAGEGKPMLILHGNGPDHRMMMSCMEPLFSSQDQYRRIYVDLPGMGMSPAAEWIRSSD 97 YE GEGKP+LI+HG PDHR+MM CME +F + Y+RIY+DLPGMG S A +WI SSD Sbjct: 12 YEVVGEGKPVLIIHGCAPDHRLMMKCMESVFQKDEGYKRIYIDLPGMGKSNAPDWINSSD 71 Query: 98 DMLRAVKMMIEALIPNERFLLVGQSYGGYLARGLLGEYAELIDGLFLLCPCVIAESSKRE 157 +L + M IE +IP + FLLVG+S+GGYLARG+L + E ++GL L+CP V+A +R Sbjct: 72 HILEVLTMFIEEIIPKKDFLLVGESFGGYLARGILSKMFERVNGLLLICPVVVAMQKERR 131 Query: 158 LPPHQVMVQDAELLKELSAADREEFISIAVVQDRTVWERFSREILCGLQLADEAFMERIK 217 LP Q++VQD E L L++ +REEF +AVV + ++RF EI GL +A+ F+ER++ Sbjct: 132 LPDKQIIVQDKEFLNTLTSTEREEFSELAVVANEYTYKRFQEEIKPGLDVANYEFIERLQ 191 Query: 218 ADGGYPFSFEADAIAPFEKPSLIITGRQDSMTGYKDVWKLLDFLPHATFAVLDRAGHNLH 277 + F +EKP L++ GRQD GY+D+ +++D P AT AVLD AGHNL Sbjct: 192 KNYSLTIDFHRKK---YEKPVLLLAGRQDISVGYQDIIEIIDGYPRATLAVLDMAGHNLQ 248 Query: 278 LEQEELFKAMAKEWLSRVSNET 299 +EQ ELF+++ EW++R + Sbjct: 249 IEQPELFESLVGEWITRTKQQN 270 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,794,734,642 Number of extensions: 73253604 Number of successful extensions: 170282 Number of sequences better than 1.0e-05: 276 Number of HSP's gapped: 170853 Number of HSP's successfully gapped: 276 Length of query: 299 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 164 Effective length of database: 1,788,859,178 Effective search space: 293372905192 Effective search space used: 293372905192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)