BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0033.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAU7.1 B3KAU7_9BACI 3-hydroxyisobutyrate dehydrogenase EC=1.1.... 398 e-109 B1DJI9.1 B1DJI9_9BACL 3-hydroxyisobutyrate dehydrogenase EC=1.1.... 307 9e-82 C0V1B2.1 C0V1B2_9BACT Beta-hydroxyacid dehydrogenase, 3-hydroxyi... 207 1e-51 C4SL85.1 C4SL85_YERFR 2-hydroxy-3-oxopropionate reductase 194 7e-48 Q1AS64.1 Q1AS64_RUBXD 2-hydroxy-3-oxopropionate reductase 193 1e-47 >B3KAU7.1 B3KAU7_9BACI 3-hydroxyisobutyrate dehydrogenase EC=1.1.1.31 Length = 298 Score = 398 bits (1022), Expect = e-109, Method: Compositional matrix adjust. Identities = 195/295 (66%), Positives = 231/295 (78%) Query: 1 MKQIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPLRAEGARVAATPQHAAEGKDV 60 MK+IGFIGLGTMGAPMASNLL+ ++VTVYNRT + PL +GA +A TP+ AAEG+D+ Sbjct: 1 MKKIGFIGLGTMGAPMASNLLKQDYDVTVYNRTHEKCRPLAEQGASIAETPREAAEGQDL 60 Query: 61 IITMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAP 120 +ITM+SND SIR+VFYG G+L L G TV+D STIS L KEIA+AV G SFLDAP Sbjct: 61 VITMVSNDQSIRDVFYGENGLLGSLTSGMTVIDCSTISESLVKEIASAVSGLGASFLDAP 120 Query: 121 VTGSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGI 180 VTGSKPAAI+GTLVFMVGG A VI+ D+FD++G+ ++HMG NGSGAVAKLAHN +VGI Sbjct: 121 VTGSKPAAIDGTLVFMVGGDAAVIEAQADVFDTLGKKVIHMGPNGSGAVAKLAHNTIVGI 180 Query: 181 HNVALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXX 240 +N+ALAEGF+IA KSG+PAD FLELV+ GSAGSK AELKG+KII+ DFSNQF Sbjct: 181 NNLALAEGFAIAAKSGIPADNFLELVQLGSAGSKAAELKGRKIIEGDFSNQFSLALMLKD 240 Query: 241 XXXXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVKSYEEWIGQKIGG 295 G+PSPML +AKS+FQAG + GYGDEDLSAVVK YE WIGQKIGG Sbjct: 241 LKLASSLTDGIGMPSPMLNLAKSLFQAGASEGYGDEDLSAVVKCYEAWIGQKIGG 295 >B1DJI9.1 B1DJI9_9BACL 3-hydroxyisobutyrate dehydrogenase EC=1.1.1.31 Length = 297 Score = 307 bits (786), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 156/294 (53%), Positives = 200/294 (68%) Query: 1 MKQIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPLRAEGARVAATPQHAAEGKDV 60 MK+IGFIGLG MG MA+NLLRSGFEV VYNRTA ++ L A G AATP A ++ Sbjct: 1 MKKIGFIGLGVMGYGMAANLLRSGFEVKVYNRTAGKAGELIACGGSEAATPADAVREAEI 60 Query: 61 IITMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAP 120 +ITMISND +IREV+YG GI ++PGT ++DSSTISP LA E+ E +G SFLDAP Sbjct: 61 VITMISNDAAIREVYYGENGIFDAVRPGTILIDSSTISPSLALELTETAESKGASFLDAP 120 Query: 121 VTGSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGI 180 VTGSKPAA GTL FMVGG + + + +MGR +++MG NGSGA AKLAHN +VGI Sbjct: 121 VTGSKPAAEGGTLTFMVGGPEKTLKEVDTVLTAMGRKVIYMGANGSGATAKLAHNTIVGI 180 Query: 181 HNVALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXX 240 + ALAEG +IA G+ FLELV++G A S+ AELKG+K++ +D+ QF Sbjct: 181 NMAALAEGMAIAASGGINGSAFLELVQSGGAASRTAELKGEKVLASDYDVQFSLALMLKD 240 Query: 241 XXXXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVKSYEEWIGQKIG 294 VP+PML AKS+FQ G + G G+ D+S+V ++YE+WIG+KIG Sbjct: 241 LRLSSVLSDGLKVPTPMLEAAKSLFQIGDSMGLGELDMSSVTQAYEQWIGRKIG 294 >C0V1B2.1 C0V1B2_9BACT Beta-hydroxyacid dehydrogenase, 3-hydroxyisobutyrate dehydrogenase Length = 288 Score = 207 bits (526), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 110/283 (38%), Positives = 165/283 (58%) Query: 1 MKQIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPLRAEGARVAATPQHAAEGKDV 60 M+++ IGLG MG+ MA NLL++GF + VYNR+ + PLR GA VA TP+ AAEG DV Sbjct: 1 MQRVALIGLGLMGSGMARNLLKAGFPLVVYNRSLEKGLPLRDAGAEVADTPRQAAEGADV 60 Query: 61 IITMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAP 120 +I+M+++D + R ++ G G L +PG +++ ST+S +E+A ++ G FLDAP Sbjct: 61 VISMVADDGASRHIWLGEAGALEGARPGMVLIECSTLSTRWVRELAGRAQDMGCQFLDAP 120 Query: 121 VTGSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGI 180 VTGS+P A G L F+VGG A ++Q I +MGR + H+G GSGA KL +N +VG+ Sbjct: 121 VTGSQPQAEAGELTFLVGGDARTLEQVEPILKAMGRTIHHLGPTGSGATMKLINNLLVGV 180 Query: 181 HNVALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXX 240 ALAE +A +G+ + L+++ NG+ GS KG K++ D+S QF Sbjct: 181 QAAALAEALHLAESAGLDTSQVLDILSNGAVGSAVVRTKGPKMLSGDYSTQFALRWMHKD 240 Query: 241 XXXXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVK 283 VP P + A+ +++ G D D +AVV+ Sbjct: 241 MTYALDEGVERTVPLPTVAAAREIYRMALARGLTDADFAAVVE 283 >C4SL85.1 C4SL85_YERFR 2-hydroxy-3-oxopropionate reductase Length = 294 Score = 194 bits (494), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 107/286 (37%), Positives = 161/286 (56%) Query: 3 QIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPLRAEGARVAATPQHAAEGKDVII 62 +IGFIGLG MG PM+ NLL++G+ +TV +R + + L + GA VAATP+ A D+II Sbjct: 2 KIGFIGLGIMGKPMSKNLLKAGYSLTVLDRNTAVLDELLSAGASVAATPKALAAECDIII 61 Query: 63 TMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAPVT 122 TM+ N ++EV G G++ KPGT ++D S+I+P +++EI+ A+ E+ + LDAPV+ Sbjct: 62 TMLPNSPHVKEVVLGENGVIEGAKPGTVLIDMSSIAPLVSREISEALAEKQVAMLDAPVS 121 Query: 123 GSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGIHN 182 G +P AI+GTL MVGG V DQ ++ +MG ++H G G+G V KLA+ +V ++ Sbjct: 122 GGEPKAIDGTLSVMVGGDKAVFDQCFEVMKAMGGSVVHTGDIGAGNVTKLANQVVVALNI 181 Query: 183 VALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXXXX 242 A++E +A K+GV D + ++ G AGS E K ++D +F F Sbjct: 182 AAMSEALVLATKAGVNPDLVFQAIRGGLAGSTVLEAKAPMVMDRNFKPGFRIDLHIKDLA 241 Query: 243 XXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVKSYEEW 288 G P+ M QA G G D SA+ YE+ Sbjct: 242 NALDTSHGVGAQLPLTAAVMEMMQALKADGLGTADHSALACYYEKL 287 >Q1AS64.1 Q1AS64_RUBXD 2-hydroxy-3-oxopropionate reductase Length = 297 Score = 193 bits (491), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 107/294 (36%), Positives = 165/294 (56%), Gaps = 1/294 (0%) Query: 2 KQIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPL-RAEGARVAATPQHAAEGKDV 60 +++GFIGLG MG PMA NL+R+G+ +TV+NRT ++E + GAR A +P+ A D+ Sbjct: 3 EKVGFIGLGIMGRPMAENLIRAGYSLTVHNRTHQKAEEFAQQTGARTAKSPKEVAGQSDI 62 Query: 61 IITMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAP 120 IITM+ + + V G GG+L ++ G+ ++D STISP +A+++AA ERG S LDAP Sbjct: 63 IITMLPDSPDVESVVAGEGGVLEGMREGSLLIDMSTISPVVARQLAAKARERGASMLDAP 122 Query: 121 VTGSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGI 180 V+G A EGTL MVGGS + R +F+ MG+ + H+G +G+G + K A+ +VG+ Sbjct: 123 VSGGDVGAREGTLSIMVGGSEEDFGRARPLFEVMGKTVTHVGPSGAGQIVKAANQIVVGL 182 Query: 181 HNVALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXX 240 A+ E + ++ GV K E++ G A ++ E+K +K + DF F Sbjct: 183 TIEAVCEALVLGLRGGVEPQKIFEVLCGGLAANRVMEVKREKFLSRDFRPGFRAELHHKD 242 Query: 241 XXXXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVKSYEEWIGQKIG 294 GV P+ V + A G+G ED SA+++ E+ G G Sbjct: 243 LGIALAAGREYGVALPVTAVVDQLLLAMRRKGWGGEDHSALLRIIEDLSGDGAG 296 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,704,026,937 Number of extensions: 69293218 Number of successful extensions: 186427 Number of sequences better than 1.0e-05: 3417 Number of HSP's gapped: 182981 Number of HSP's successfully gapped: 3426 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)