BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0027.p (265 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAV4.1 B3KAV4_9BACI Sulfate ABC transporter, inner membrane su... 404 e-111 B1DJJ4.1 B1DJJ4_9BACL Sulfate ABC transporter, inner membrane su... 361 5e-98 B0THG4.1 B0THG4_HELMI Sulfate abc transporter, permease protein ... 216 1e-54 A9B6K6.1 A9B6K6_HERA2 Sulfate ABC transporter, inner membrane su... 196 2e-48 B5W877.1 B5W877_SPIMA Sulfate ABC transporter, inner membrane su... 194 8e-48 >B3KAV4.1 B3KAV4_9BACI Sulfate ABC transporter, inner membrane subunit Length = 265 Score = 404 bits (1038), Expect = e-111, Method: Compositional matrix adjust. Identities = 196/265 (73%), Positives = 221/265 (83%) Query: 1 MRKLWIGLTYLVFFLLIAAPLGKMTIGAFSEGFDGFWNALTRPEALHALMMTGFXXXXXT 60 M++LWIGLTY VF +L+ PL ++ IGAF +G DGF L RPEALHALMMTG T Sbjct: 1 MKRLWIGLTYAVFIILLIIPLSRIAIGAFDKGLDGFAEGLLRPEALHALMMTGLIVIFVT 60 Query: 61 LLNTLFGIMMALYLVRASWLGSRVKGLLNSIVDLPYAVSPXXXXXXXXXXXXPESALGAV 120 LN LFGIMMALYLVRA+WL ++KG+LNSIVDLPYAVSP P+S +G Sbjct: 61 FLNALFGIMMALYLVRANWLSRKIKGILNSIVDLPYAVSPVIGGLMIVLLLGPDSIIGGF 120 Query: 121 FEQIGLKIVYAFPGMVIATLFVTFPLMVREIMPVLQEIGSQQEEAASTLGAYGWTTFWKV 180 FE IG K+VYAFPGMVIATLFVTFPLMVRE+MPVLQEIG+ QE+AA+TLGAYGWTTF KV Sbjct: 121 FEGIGFKVVYAFPGMVIATLFVTFPLMVREVMPVLQEIGNDQEQAAATLGAYGWTTFVKV 180 Query: 181 TWPSIRWAVIYGVILTVARSLGEFGAVLVVSGNIMNKTQTATTLVYQDVENFNVTAAGGI 240 TWPSIRWAV+YG++LTVARSLGEFGAVLVVSGNIMNKTQTATTLVYQDVENFNV AA G+ Sbjct: 181 TWPSIRWAVVYGLVLTVARSLGEFGAVLVVSGNIMNKTQTATTLVYQDVENFNVAAANGV 240 Query: 241 ALVLAAFSAGLLLLMEWSKRRKEVH 265 ALVLAAFS GLLLLMEW+K+RK+VH Sbjct: 241 ALVLAAFSVGLLLLMEWAKKRKDVH 265 >B1DJJ4.1 B1DJJ4_9BACL Sulfate ABC transporter, inner membrane subunit Length = 267 Score = 361 bits (926), Expect = 5e-98, Method: Compositional matrix adjust. Identities = 180/265 (67%), Positives = 214/265 (80%) Query: 1 MRKLWIGLTYLVFFLLIAAPLGKMTIGAFSEGFDGFWNALTRPEALHALMMTGFXXXXXT 60 +R+ WIGLTY+VF LL+ PL ++ G++++G+ GF LTR ++LHAL MT T Sbjct: 3 IRRTWIGLTYIVFTLLLIIPLIEIFTGSWADGWSGFVEGLTRKQSLHALQMTAIIVVVVT 62 Query: 61 LLNTLFGIMMALYLVRASWLGSRVKGLLNSIVDLPYAVSPXXXXXXXXXXXXPESALGAV 120 +NT+FG+M+ALYLVRA W+G R+K LLNSIVDLP+AVSP P + +G+ Sbjct: 63 AINTVFGVMLALYLVRAHWIGPRMKRLLNSIVDLPFAVSPVIGGLMIVLLLGPNTIIGSF 122 Query: 121 FEQIGLKIVYAFPGMVIATLFVTFPLMVREIMPVLQEIGSQQEEAASTLGAYGWTTFWKV 180 FE G K+VYA PGM++A+LFVTFPLMVRE++PVLQEIG+QQEEAASTLGAY W TFWKV Sbjct: 123 FEDTGFKVVYALPGMILASLFVTFPLMVREVLPVLQEIGAQQEEAASTLGAYSWYTFWKV 182 Query: 181 TWPSIRWAVIYGVILTVARSLGEFGAVLVVSGNIMNKTQTATTLVYQDVENFNVTAAGGI 240 TWPSIRW VIYGV+LTVAR+LGEFGAVLVVSGNIMNKTQTATTLVYQDVENFNV A GI Sbjct: 183 TWPSIRWGVIYGVVLTVARTLGEFGAVLVVSGNIMNKTQTATTLVYQDVENFNVVEANGI 242 Query: 241 ALVLAAFSAGLLLLMEWSKRRKEVH 265 ALVLA FS GLLLLMEW+K+RKEVH Sbjct: 243 ALVLAVFSVGLLLLMEWAKKRKEVH 267 >B0THG4.1 B0THG4_HELMI Sulfate abc transporter, permease protein cysw Length = 274 Score = 216 bits (551), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 119/257 (46%), Positives = 167/257 (64%), Gaps = 10/257 (3%) Query: 5 WIGLTYLVFFLLIAAPLGKMTIGAFSEGFDGFWNALTRPEALHALMMTGFXXXXXTLLNT 64 WIG+ L+ AP+G + AF+ G F + RPEA HAL ++ L+N Sbjct: 22 WIGI-------LLIAPVGALLSEAFAGGAAPFLREILRPEAFHALQVSAGITMAVVLING 74 Query: 65 LFGIMMALYLVRASWLGSRVKGLLNSIVDLPYAVSPXXXXXXXXXXXXPESALGAVFEQI 124 +FG+M+A L R + G + L+N++VDLP+AVSP P LG F Sbjct: 75 IFGVMLAFVLTRHHFPGRK---LINNMVDLPFAVSPVIAGLMLVLVYGPNQLLGTFFGLW 131 Query: 125 GLKIVYAFPGMVIATLFVTFPLMVREIMPVLQEIGSQQEEAASTLGAYGWTTFWKVTWPS 184 G+K+++A PGM+ ATLFVT PLM R++ P+L+ +G+QQEEAA TLGA W TFW++T PS Sbjct: 132 GVKVIFAIPGMIAATLFVTLPLMTRQLAPLLETLGTQQEEAAYTLGANRWQTFWRITLPS 191 Query: 185 IRWAVIYGVILTVARSLGEFGAVLVVSGNIMNKTQTATTLVYQDVENFNVTAAGGIALVL 244 IRW + YG ++T AR+LGEFGAVLVVSGN++++TQ+AT +YQ +FN+ A +A+VL Sbjct: 192 IRWGLFYGAVMTAARALGEFGAVLVVSGNLIHETQSATLYIYQAAADFNLQGAYAVAVVL 251 Query: 245 AAFSAGLLLLMEWSKRR 261 AA + LL++MEW K R Sbjct: 252 AAMAIALLVVMEWLKGR 268 >A9B6K6.1 A9B6K6_HERA2 Sulfate ABC transporter, inner membrane subunit Length = 269 Score = 196 bits (497), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 106/249 (42%), Positives = 153/249 (61%), Gaps = 3/249 (1%) Query: 15 LLIAAPLGKMTIGAFSEGFDGFWNALTRPEALHALMMTGFXXXXXTLLNTLFGIMMALYL 74 +LI APLG + GAF EG AL P L + +T ++ +FG ++A L Sbjct: 18 ILIIAPLGAIVFGAFREGAPAVLEALQHPHVLRSFGLTLIICLVVVSIHGIFGTLVAWVL 77 Query: 75 VRASWLGSRVKGLLNSIVDLPYAVSPXXXXXXXXXXXXPESALGAVFEQIGLKIVYAFPG 134 VR ++G K L+N ++DLP+A+SP L + E + +I +A PG Sbjct: 78 VRHQFVG---KALVNRLLDLPFAMSPVIVGFTLIVLFGRNGVLKPLTEALDWQIAFALPG 134 Query: 135 MVIATLFVTFPLMVREIMPVLQEIGSQQEEAASTLGAYGWTTFWKVTWPSIRWAVIYGVI 194 M++ATLFVT P MVRE+MPVL+ G +QEEAA+TLGA GW TF VT+P++RWA +YG+ Sbjct: 135 MILATLFVTLPFMVRELMPVLEAFGRKQEEAAATLGASGWQTFRLVTFPALRWAFLYGLG 194 Query: 195 LTVARSLGEFGAVLVVSGNIMNKTQTATTLVYQDVENFNVTAAGGIALVLAAFSAGLLLL 254 LT AR+LGEFGAVLV+SG I +T+TAT +++ +++ A AL+L A S L+L+ Sbjct: 195 LTCARALGEFGAVLVISGGIQGRTETATVYIFRALDDRQYAGAYSAALLLGAISLVLVLV 254 Query: 255 MEWSKRRKE 263 ++ RR + Sbjct: 255 IDVFHRRSQ 263 >B5W877.1 B5W877_SPIMA Sulfate ABC transporter, inner membrane subunit Length = 288 Score = 194 bits (492), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 102/248 (41%), Positives = 153/248 (61%), Gaps = 3/248 (1%) Query: 15 LLIAAPLGKMTIGAFSEGFDGFWNALTRPEALHALMMTGFXXXXXTLLNTLFGIMMALYL 74 L++ P + +GAF G+ GF A+T PE LHA ++ ++ +FGI+ AL + Sbjct: 42 LVLFVPAANIFVGAFRNGWSGFARAVTSPEFLHAAKLSVMIDAIVVPVSVIFGIVTALAI 101 Query: 75 VRASWLGSRVKGLLNSIVDLPYAVSPXXXXXXXXXXXXPESALGAVFEQIGLKIVYAFPG 134 R + G + LL SI+DLP+++SP + GA + ++I++AFPG Sbjct: 102 ARHQFPG---RTLLLSIIDLPFSISPVVVGLMLVLLYGRQGWFGAALQSANIRILFAFPG 158 Query: 135 MVIATLFVTFPLMVREIMPVLQEIGSQQEEAASTLGAYGWTTFWKVTWPSIRWAVIYGVI 194 MV+A+ FV P + RE++P L+EIG++QEEAA T+GA W TFW+VT PSIRW+++YG++ Sbjct: 159 MVLASAFVCMPFVAREVLPALEEIGTEQEEAAKTMGANDWQTFWRVTLPSIRWSLLYGIL 218 Query: 195 LTVARSLGEFGAVLVVSGNIMNKTQTATTLVYQDVENFNVTAAGGIALVLAAFSAGLLLL 254 LT ARS+GEFGAV VVSGNI+ KTQT V + + TA+ A+VL + S L+ Sbjct: 219 LTNARSMGEFGAVAVVSGNIIGKTQTLPLFVEEMHVQYQTTASYSAAVVLGSLSFVTLVF 278 Query: 255 MEWSKRRK 262 + + +K Sbjct: 279 KKLLEAKK 286 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.139 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,377,727,908 Number of extensions: 49298361 Number of successful extensions: 226024 Number of sequences better than 1.0e-05: 3240 Number of HSP's gapped: 225646 Number of HSP's successfully gapped: 3251 Length of query: 265 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 132 Effective length of database: 1,807,701,208 Effective search space: 238616559456 Effective search space used: 238616559456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)