BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0025.p (338 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAV6.1 B3KAV6_9BACI Sulfate ABC transporter, periplasmic sulfa... 487 e-136 B1DJJ6.1 B1DJJ6_9BACL Sulfate ABC transporter, periplasmic sulfa... 473 e-132 B0THG6.1 B0THG6_HELMI Sulfate abc transporter, sulfate-binding p... 348 3e-94 C1PVR9.1 C1PVR9_ALIAC Sulfate/thiosulfate-binding protein 305 3e-81 A9B6K8.1 A9B6K8_HERA2 Sulfate ABC transporter, periplasmic sulfa... 301 6e-80 >B3KAV6.1 B3KAV6_9BACI Sulfate ABC transporter, periplasmic sulfate-binding protein Length = 352 Score = 487 bits (1253), Expect = e-136, Method: Compositional matrix adjust. Identities = 230/328 (70%), Positives = 275/328 (83%), Gaps = 1/328 (0%) Query: 11 GCGNGKESTAAEKPQQGDLTLVVGAYSVAKDAMGEILPLFAAAWKAKTGQTLTFQQSYEA 70 GC + + Q G+ T+V+GAYSVA+DA+ EI+P F A WKA+TG+ + FQ+SYEA Sbjct: 26 GCADDQPVNEDAISQPGERTIVLGAYSVARDALSEIIPAFQAEWKAQTGEIIRFQESYEA 85 Query: 71 SGTQARAIAGGFEADVTLLAMEGDVDKLVKAGLVEADWKDRGVNGMVTRSVVALGTREGN 130 SGTQARAI GGFEADVTLLAMEGDVDKLV+AGLV+ +WK + NGMVTRS+V LGTR GN Sbjct: 86 SGTQARAITGGFEADVTLLAMEGDVDKLVRAGLVDKEWKSQPYNGMVTRSIVVLGTRAGN 145 Query: 131 PKGIHDFADLAKPGVKVLYPNPKTSGGAQWDINAIYGAGLKLSEEQEGAKDPAAAKAFLE 190 PKGI DF DL + VKVLYPNPKTSGGAQWDINAIYGAGLK SEE G +DPA AKAFLE Sbjct: 146 PKGIRDFDDLTRSDVKVLYPNPKTSGGAQWDINAIYGAGLKQSEEN-GKRDPAEAKAFLE 204 Query: 191 SVHANVESLDKSGRASMAAFEYGVGDVIVTYENELLARIAQGVKYEVIIPRNTILIENPA 250 +VH NVESLDKSGRASMAAFEYGVGDVIVTYENELLARIAQGV+Y++++P++T+LIENPA Sbjct: 205 AVHRNVESLDKSGRASMAAFEYGVGDVIVTYENELLARIAQGVQYDIVVPQHTMLIENPA 264 Query: 251 AVVNKYAEEHGTSEAAQALVDYLTTPEAQQVFAKYGFRPVDEQVYAENEGRYPVPAGLFD 310 AVV ++A++HGT + + A V+YL TPEAQ +F K+GFRPV+E V+ + + R+ P GLFD Sbjct: 265 AVVERFAKKHGTEDVSAAFVEYLRTPEAQNIFVKHGFRPVEESVFEQTKERFANPPGLFD 324 Query: 311 ISYLGGWDKVRTTLYSKRGIWYQVLAGI 338 I YLGGWD+VR TLYSKRG+WYQVLAG+ Sbjct: 325 IEYLGGWDQVRETLYSKRGVWYQVLAGL 352 >B1DJJ6.1 B1DJJ6_9BACL Sulfate ABC transporter, periplasmic sulfate-binding protein Length = 353 Score = 473 bits (1218), Expect = e-132, Method: Compositional matrix adjust. Identities = 229/331 (69%), Positives = 268/331 (80%), Gaps = 5/331 (1%) Query: 11 GCGNGKEST---AAEKPQQGDLTLVVGAYSVAKDAMGEILPLFAAAWKAKTGQTLTFQQS 67 GCG KE T A Q GD+TLV+GAYSV KDA EILP F A WK +TGQ + FQ+S Sbjct: 25 GCG--KEETKQPAVSTAQDGDVTLVIGAYSVVKDAFSEILPQFQAYWKEQTGQKVVFQES 82 Query: 68 YEASGTQARAIAGGFEADVTLLAMEGDVDKLVKAGLVEADWKDRGVNGMVTRSVVALGTR 127 YEASGTQARAIAGGFEADV +LAMEGD+DK+ AG + DWK + G++T+S+V LGTR Sbjct: 83 YEASGTQARAIAGGFEADVAILAMEGDLDKISGAGFITHDWKSQPNKGLITKSIVVLGTR 142 Query: 128 EGNPKGIHDFADLAKPGVKVLYPNPKTSGGAQWDINAIYGAGLKLSEEQEGAKDPAAAKA 187 EGNPKGI D+ DL + GVKVLYPNPKTSGGAQWDINAIYGAGLK SEE+ G KDPA AK Sbjct: 143 EGNPKGIKDWDDLTRKGVKVLYPNPKTSGGAQWDINAIYGAGLKKSEEETGKKDPAFAKQ 202 Query: 188 FLESVHANVESLDKSGRASMAAFEYGVGDVIVTYENELLARIAQGVKYEVIIPRNTILIE 247 +LES+H NVESLDKSGRASMAAFEYGVGDVIVTYENELL RI GVKY+V++P+NTILIE Sbjct: 203 YLESIHKNVESLDKSGRASMAAFEYGVGDVIVTYENELLGRIMNGVKYDVVVPKNTILIE 262 Query: 248 NPAAVVNKYAEEHGTSEAAQALVDYLTTPEAQQVFAKYGFRPVDEQVYAENEGRYPVPAG 307 NPAAVV+K ++HG+ + A+A V YL + EAQ+ F KYGFR VD+++ E++ +Y VP G Sbjct: 263 NPAAVVDKNVDKHGSRKVAEAFVSYLQSEEAQRTFVKYGFRSVDDKIAEESKDKYVVPEG 322 Query: 308 LFDISYLGGWDKVRTTLYSKRGIWYQVLAGI 338 LFDISYLGGW +VR LYSK+GIWYQVLAGI Sbjct: 323 LFDISYLGGWKEVRQNLYSKKGIWYQVLAGI 353 >B0THG6.1 B0THG6_HELMI Sulfate abc transporter, sulfate-binding protein, putative Length = 346 Score = 348 bits (894), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 165/308 (53%), Positives = 216/308 (70%), Gaps = 1/308 (0%) Query: 31 LVVGAYSVAKDA-MGEILPLFAAAWKAKTGQTLTFQQSYEASGTQARAIAGGFEADVTLL 89 +++ AY+V K+A EI+P F WK KTGQT+TF++SY SG QARAI G EADV L Sbjct: 35 IMLAAYTVPKEAYQKEIIPAFQKQWKEKTGQTVTFKESYVGSGAQARAIVNGLEADVAAL 94 Query: 90 AMEGDVDKLVKAGLVEADWKDRGVNGMVTRSVVALGTREGNPKGIHDFADLAKPGVKVLY 149 ++EGD++ + KAGL+ DWK R NGM+TRSVVA G ++GNP I ++ DL KPG+ VLY Sbjct: 95 SLEGDIETIHKAGLITHDWKQRPHNGMITRSVVAFGVKKGNPLTIREWQDLTKPGIAVLY 154 Query: 150 PNPKTSGGAQWDINAIYGAGLKLSEEQEGAKDPAAAKAFLESVHANVESLDKSGRASMAA 209 P+P+TSGGA WDINAIYGAGLK++E + G KDP + L ++ NV+ +D SGR SM Sbjct: 155 PSPRTSGGAMWDINAIYGAGLKMAEMETGKKDPDRGRELLSAIQKNVKVMDNSGRESMTT 214 Query: 210 FEYGVGDVIVTYENELLARIAQGVKYEVIIPRNTILIENPAAVVNKYAEEHGTSEAAQAL 269 FE G+GDV+VTYENELL R + Y+VI P+ TILIENP A+V+K ++HG +A Sbjct: 215 FEKGLGDVVVTYENELLLRNMEQPLYDVIYPKATILIENPVALVDKNVDKHGNRAVVEAF 274 Query: 270 VDYLTTPEAQQVFAKYGFRPVDEQVYAENEGRYPVPAGLFDISYLGGWDKVRTTLYSKRG 329 V YL + EAQ+ FAKYGFRPVD V AE + ++P P LFDI YLGGW +Y +G Sbjct: 275 VAYLQSTEAQRAFAKYGFRPVDPTVMAETKDKFPEPEWLFDIDYLGGWATASEQIYGPQG 334 Query: 330 IWYQVLAG 337 +W ++ G Sbjct: 335 VWTGIVEG 342 >C1PVR9.1 C1PVR9_ALIAC Sulfate/thiosulfate-binding protein Length = 355 Score = 305 bits (782), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 153/310 (49%), Positives = 206/310 (66%), Gaps = 8/310 (2%) Query: 29 LTLVVGAYSVAKDAMGE-ILPLFAAAWKAKTGQTLTFQQSYEASGTQARAIAGGFEADVT 87 +TL G+YSV + + + P+F WK KTGQT+ FQ S++ASG +A AIA G DV Sbjct: 52 VTLYFGSYSVTQQIYEQKLFPMFQKYWKQKTGQTVRFQASFDASGAEATAIANGLPVDVA 111 Query: 88 LLAMEGDVDKLVKAGLVEADWKDRGVNGMVTRSVVALGTREGNPKGIHDFADLAKPGVKV 147 L++E D+DK+ KAGL+ +WKD +GMVT S+VA+ R+GNPK I +ADLAKPGV V Sbjct: 112 ALSLESDIDKIQKAGLITHNWKDTPTHGMVTDSIVAIAVRKGNPKHIETWADLAKPGVIV 171 Query: 148 LYPNPKTSGGAQWDINAIYGAGLKLSEEQEGAKDPAAAKAFLESVHANVESLDKSGRASM 207 PNP TSGGA+WDINAIY A L+ E AK+ L ++ NV+ LDKSG ASM Sbjct: 172 DLPNPATSGGAKWDINAIYYATLRHGSASE-------AKSLLAAIVKNVQVLDKSGEASM 224 Query: 208 AAFEYGVGDVIVTYENELLARIAQGVKYEVIIPRNTILIENPAAVVNKYAEEHGTSEAAQ 267 F GVGD V+YENE+L + ++ +IP +T+LIENP A+V+K A+ HG + AQ Sbjct: 225 TTFVNGVGDACVSYENEILEKSNHLKTFDEVIPSSTMLIENPIALVDKNADAHGDRQVAQ 284 Query: 268 ALVDYLTTPEAQQVFAKYGFRPVDEQVYAENEGRYPVPAGLFDISYLGGWDKVRTTLYSK 327 A +++L P+AQ+VF +YGFRPV+ + AE E ++ PA LF + +GGW + TLY + Sbjct: 285 AFINWLEGPDAQRVFMQYGFRPVNPSLKAEAEKQFKTPADLFTAADVGGWSSINKTLYGQ 344 Query: 328 RGIWYQVLAG 337 GIW QVL G Sbjct: 345 NGIWNQVLEG 354 >A9B6K8.1 A9B6K8_HERA2 Sulfate ABC transporter, periplasmic sulfate-binding protein Length = 345 Score = 301 bits (771), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 146/308 (47%), Positives = 205/308 (66%) Query: 31 LVVGAYSVAKDAMGEILPLFAAAWKAKTGQTLTFQQSYEASGTQARAIAGGFEADVTLLA 90 + +GAY+ ++A +++PLF A WKA TG + F++SY+ SG Q+RAI GFEAD+ L+ Sbjct: 36 ITLGAYTTPREAYAKLIPLFQAKWKADTGGEVKFEESYQGSGAQSRAIVEGFEADIAALS 95 Query: 91 MEGDVDKLVKAGLVEADWKDRGVNGMVTRSVVALGTREGNPKGIHDFADLAKPGVKVLYP 150 +E D++++ AGL+ DWK +GMV+ S+V REGNPKGI D+ADLAKPGV++L P Sbjct: 96 LEADINRITDAGLITHDWKAGTHSGMVSTSIVVFAVREGNPKGIQDWADLAKPGVQILTP 155 Query: 151 NPKTSGGAQWDINAIYGAGLKLSEEQEGAKDPAAAKAFLESVHANVESLDKSGRASMAAF 210 +P+TSGGAQW+I A+YGA + A D AAA+AFL SV NV DK R S+ F Sbjct: 156 DPRTSGGAQWNILALYGAAKRGQITGVPANDEAAAQAFLASVLKNVVVFDKGARESITNF 215 Query: 211 EYGVGDVIVTYENELLARIAQGVKYEVIIPRNTILIENPAAVVNKYAEEHGTSEAAQALV 270 E GVGDV +TYENE+L G Y+++IP +TILIENP A+++K E+HG +A +A + Sbjct: 216 EKGVGDVAITYENEILVGQKGGQTYQMVIPTSTILIENPIALIDKSVEKHGNRQAVEAFI 275 Query: 271 DYLTTPEAQQVFAKYGFRPVDEQVYAENEGRYPVPAGLFDISYLGGWDKVRTTLYSKRGI 330 ++L + EAQ+VFA++G R VD V RYP LF I+ GGW K + G+ Sbjct: 276 NFLHSREAQEVFAEFGLRSVDADVAKATAERYPAINDLFTINEFGGWSKATPEYFGDDGV 335 Query: 331 WYQVLAGI 338 + +VLA + Sbjct: 336 YAKVLAQV 343 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,990,987,110 Number of extensions: 83524818 Number of successful extensions: 168059 Number of sequences better than 1.0e-05: 864 Number of HSP's gapped: 165295 Number of HSP's successfully gapped: 865 Length of query: 338 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 202 Effective length of database: 1,779,438,163 Effective search space: 359446508926 Effective search space used: 359446508926 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)