BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0022.p (256 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5CIF5.1 C5CIF5_9THEM Short-chain dehydrogenase/reductase SDR 194 4e-48 Q2RUY0.1 Q2RUY0_RHORT Short-chain dehydrogenase/reductase SDR EC... 192 3e-47 B9JM87.1 B9JM87_AGRRK Short chain dehydrogenase protein 183 1e-44 Q9KA03.1 Q9KA03_BACHD 3-oxoacyl-(Acyl-carrier protein) reductase... 170 1e-40 A3IG79.1 A3IG79_9BACI 3-oxoacyl-(Acyl-carrier protein) reductase 169 2e-40 >C5CIF5.1 C5CIF5_9THEM Short-chain dehydrogenase/reductase SDR Length = 263 Score = 194 bits (494), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 108/236 (45%), Positives = 151/236 (63%), Gaps = 16/236 (6%) Query: 28 FLKAGANVAIGDIACRPGLERIHDRLVQIQ----------MDVTISADAARLIDAAVEAF 77 F K GA +A+ DI E +++ L +I+ +DVT +++ +E F Sbjct: 29 FAKEGAKLALADI----NKEVLNETLGEIKQYSPDSRAYVLDVTNETLVKNVVEIVLEDF 84 Query: 78 GGVDFLVNNSGTSTMDYAVDIREEDWDKVMDINAKGVYLVSQAGARQMLRQGRGGRIINI 137 G +D L+NN+G STM++ ++ EE+WD M++NAKGV+LVS+ A M+++ R G+I+N Sbjct: 85 GTIDILINNAGVSTMNWFWELTEEEWDYNMNVNAKGVWLVSKHVAPHMIKK-RKGKIVNT 143 Query: 138 ASQAGKNGYRCMGNYVASKHAVLGFTKVMALELAQAQILVNAVCPGIVETDMKRRERVEG 197 AS A K G + +Y ASK AV+GFT+ ++ ELA I VNAVCPG V+T M+ RE V Sbjct: 144 ASMASKIGAPLLAHYSASKFAVIGFTQAISKELAPYNINVNAVCPGFVKTSMQDREIVWE 203 Query: 198 AALRGM-QPEDILAEDRSQVPLGRTAEPEDVANVVLFLASPLSSYMTGQAINVTGG 252 A LRG+ +PE + E Q PLGR PEDVA VVLFLAS + ++TGQAINVTGG Sbjct: 204 AKLRGIDEPEKVREEYIKQTPLGRLCYPEDVAKVVLFLASEDADFLTGQAINVTGG 259 >Q2RUY0.1 Q2RUY0_RHORT Short-chain dehydrogenase/reductase SDR EC=1.1.1.100 Length = 260 Score = 192 bits (487), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 99/187 (52%), Positives = 126/187 (67%) Query: 70 IDAAVEAFGGVDFLVNNSGTSTMDYAVDIREEDWDKVMDINAKGVYLVSQAGARQMLRQG 129 +D A+ + GG+D LV N+G S+M AV++ +EDW D+NAKGV+L +Q R L G Sbjct: 74 VDRAIASCGGIDILVANAGVSSMRKAVELTDEDWAFNFDVNAKGVFLANQLVVRHFLATG 133 Query: 130 RGGRIINIASQAGKNGYRCMGNYVASKHAVLGFTKVMALELAQAQILVNAVCPGIVETDM 189 R G I+N AS A K G + +Y ASK AVLG+T+ +A ELA I VNAVCPG V T M Sbjct: 134 RKGVIVNTASLAAKVGAPLLAHYAASKFAVLGWTQSLARELAPEGIRVNAVCPGFVRTPM 193 Query: 190 KRRERVEGAALRGMQPEDILAEDRSQVPLGRTAEPEDVANVVLFLASPLSSYMTGQAINV 249 + RE + LRGM PED+ E + PLGR EPEDVA+V+LFLAS LS +MTGQ INV Sbjct: 194 QDREIIWEGELRGMTPEDVRKEYVALTPLGRIEEPEDVADVILFLASDLSRFMTGQGINV 253 Query: 250 TGGMTMN 256 TGG+ M+ Sbjct: 254 TGGIKMD 260 >B9JM87.1 B9JM87_AGRRK Short chain dehydrogenase protein Length = 261 Score = 183 bits (465), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 105/221 (47%), Positives = 134/221 (60%), Gaps = 14/221 (6%) Query: 37 IGDIACRPGLERIHDR-LVQIQMDVTISADAARLIDAAVEAFGGVDFLVNNSGTSTMDYA 95 +GD A G++ + DR VQ MD IS GG+D L N+G STM + Sbjct: 54 LGDNAFSGGID-VRDRGFVQAAMDAVISGQ------------GGIDILCANAGVSTMQAS 100 Query: 96 VDIREEDWDKVMDINAKGVYLVSQAGARQMLRQGRGGRIINIASQAGKNGYRCMGNYVAS 155 VD+ +EDWD MD+NAKGV+L +Q R L G G I+N AS AGK G + +Y AS Sbjct: 101 VDLTDEDWDFNMDVNAKGVFLCNQIVVRHFLATGNKGVIVNTASLAGKVGAPLLAHYSAS 160 Query: 156 KHAVLGFTKVMALELAQAQILVNAVCPGIVETDMKRRERVEGAALRGMQPEDILAEDRSQ 215 K AVLG+T+ +A ELA I VNAVCPG V T M+ RE + LR M P+++ E + Sbjct: 161 KFAVLGWTQALARELAPTGIRVNAVCPGFVRTGMQEREIIWEGKLRNMTPDEVRQEYVTL 220 Query: 216 VPLGRTAEPEDVANVVLFLASPLSSYMTGQAINVTGGMTMN 256 P+GR EPEDVA VV FLAS + +MTGQ INVTGG+ M+ Sbjct: 221 TPMGRIEEPEDVAVVVRFLASDGARFMTGQGINVTGGVRMD 261 >Q9KA03.1 Q9KA03_BACHD 3-oxoacyl-(Acyl-carrier protein) reductase EC=1.1.1.100 Length = 246 Score = 170 bits (430), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 97/233 (41%), Positives = 133/233 (57%), Gaps = 19/233 (8%) Query: 30 KAGANVAIGDIACRPGLERIHDRL-------VQIQMDVTISADAARLIDAAVEAFGGVDF 82 + GANV + + E++ + + IQ DV S ++ ++ FG VD Sbjct: 26 RHGANVVVNYAGNKEKAEKVVAEIKELGVEAIAIQADVADSESVQAMVKETIDTFGAVDI 85 Query: 83 LVNNSGTSTMDYAVDIREEDWDKVMDINAKGVYLVSQAGARQMLRQGRGGRIINIASQAG 142 LVNN+G + + + ++EEDWD V+D N KGV+ S+A R M++Q R GRIIN++S G Sbjct: 86 LVNNAGITRDNLFMRMKEEDWDAVIDTNLKGVFHCSKAVTRPMMKQ-RFGRIINVSSVVG 144 Query: 143 KNGYRCMGNYVASKHAVLGFTKVMALELAQAQILVNAVCPGIVETDMKRRERVEGAALRG 202 G NYVA+K V+G TK +A ELA I VNAV PG +ETDM G Sbjct: 145 AIGNAGQANYVAAKAGVIGLTKTLARELANRNITVNAVAPGFIETDMT-----------G 193 Query: 203 MQPEDILAEDRSQVPLGRTAEPEDVANVVLFLASPLSSYMTGQAINVTGGMTM 255 PED+ A+ Q+PL R +PE+VA V FLAS +SY+TGQ I+V GGM M Sbjct: 194 ELPEDVKAQMLGQIPLARLGQPEEVAKAVRFLASDDASYLTGQTIHVNGGMVM 246 >A3IG79.1 A3IG79_9BACI 3-oxoacyl-(Acyl-carrier protein) reductase Length = 248 Score = 169 bits (428), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 87/202 (43%), Positives = 127/202 (62%), Gaps = 12/202 (5%) Query: 54 VQIQMDVTISADAARLIDAAVEAFGGVDFLVNNSGTSTMDYAVDIREEDWDKVMDINAKG 113 + +Q V+ + + L+D AV+ FG +D LVNN+G + + + ++E++WD V+D N KG Sbjct: 59 IAVQASVSQTEEVTALMDTAVKTFGSLDILVNNAGITRDNLLMRMKEDEWDDVLDTNLKG 118 Query: 114 VYLVSQAGARQMLRQGRGGRIINIASQAGKNGYRCMGNYVASKHAVLGFTKVMALELAQA 173 V+L ++A RQM++Q R GRIINI+S G G NYVA+K V+G TK A ELA Sbjct: 119 VFLCTKAVTRQMMKQ-RAGRIINISSIVGVAGNAGQANYVAAKAGVIGLTKTTAKELASR 177 Query: 174 QILVNAVCPGIVETDMKRRERVEGAALRGMQPEDILAEDRSQVPLGRTAEPEDVANVVLF 233 ILVNA+ PG +ET+M + PEDI +Q+PL + +PED+A V+F Sbjct: 178 NILVNAIAPGFIETEMTDQ-----------LPEDIKQGMLTQIPLAKLGQPEDIAKAVVF 226 Query: 234 LASPLSSYMTGQAINVTGGMTM 255 LAS ++YMTGQ +++ GGM M Sbjct: 227 LASEDANYMTGQTLHIDGGMVM 248 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,269,746,436 Number of extensions: 46770316 Number of successful extensions: 203736 Number of sequences better than 1.0e-05: 26332 Number of HSP's gapped: 167046 Number of HSP's successfully gapped: 26408 Length of query: 256 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 123 Effective length of database: 1,807,701,208 Effective search space: 222347248584 Effective search space used: 222347248584 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)