BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0018.p (341 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4US85.1 C4US85_YERRO Methionine import ATP-binding protein metN 2 452 e-125 C4T1U9.1 C4T1U9_YERIN Methionine import ATP-binding protein metN 2 442 e-122 C4RXH1.1 C4RXH1_YERBE Methionine import ATP-binding protein metN 2 442 e-122 A1JT91.1 A1JT91_YERE8 Putative ABC transport family protein 439 e-121 C4SE98.1 C4SE98_YERMO Methionine import ATP-binding protein metN 2 437 e-121 >C4US85.1 C4US85_YERRO Methionine import ATP-binding protein metN 2 Length = 338 Score = 452 bits (1163), Expect = e-125, Method: Compositional matrix adjust. Identities = 215/340 (63%), Positives = 282/340 (82%), Gaps = 4/340 (1%) Query: 1 MIEIKNVYKTFERKGQSIEALRGVSLSIARGDIYGVIGYSGAGKSTLIRLVNALERPTAG 60 MIE++++YKTFERKG ++AL VSL++ GDI+G+IGYSGAGKSTL+R+VN+LE PT+G Sbjct: 1 MIELQHIYKTFERKGTKLQALNDVSLTVEDGDIFGIIGYSGAGKSTLLRMVNSLENPTSG 60 Query: 61 EVLVEGQDLAGYNPAELRQAKKNIGMIFQHFNLLESKTVFDNIAIPLVLLKRSKQEIRER 120 V+++G+DL ++ +LR KKNIGMIFQ+FNLLESKTVF N+A+PL+LL ++K+ I+ER Sbjct: 61 SVVIDGKDLHNFSHEQLRLLKKNIGMIFQNFNLLESKTVFKNVAMPLILLGKNKKFIQER 120 Query: 121 VTELLKFTGLGDKANSYPKELSGGQKQRVGIARALASNPSILLCDEATSALDPQTTKSIL 180 V ELL F GLGDK++S+P ELSGGQKQRVGIARALA+NPSILLCDEATS+LDPQTT IL Sbjct: 121 VAELLDFVGLGDKSHSFPNELSGGQKQRVGIARALATNPSILLCDEATSSLDPQTTAQIL 180 Query: 181 ELLQKINEEYNITIMIITHEMAVIQQICNKVAVMERGEIIEQGNVLDVFGSPHHPTTQSF 240 LL+KIN++YNIT+++ITHEM+VIQ+ICNKVAVME G+IIEQG+VL VFG P HPTT +F Sbjct: 181 LLLKKINQQYNITVLLITHEMSVIQKICNKVAVMENGQIIEQGSVLAVFGHPTHPTTINF 240 Query: 241 VQTVIHNSVPQSVLHTLKAENGRRLFKLKFXXXXGA-AWEPIINSLIRRYEVDVNILFAN 299 V+TVI +S+P SV L + R FKL F GA A +P+IN LI++Y + VNILFAN Sbjct: 241 VRTVIKDSLPDSVKKLLDNSHAGRQFKLAF---MGAIATQPVINQLIKQYAIGVNILFAN 297 Query: 300 MTEIQNTTLGNMILQMHGDHAVIDRAADFITSQGVEITEV 339 M+EIQ+TTLG+M+L + G+++ IDRA ++T G++I E+ Sbjct: 298 MSEIQDTTLGHMVLLLSGENSTIDRAVTYLTQTGIDIQEI 337 >C4T1U9.1 C4T1U9_YERIN Methionine import ATP-binding protein metN 2 Length = 338 Score = 442 bits (1137), Expect = e-122, Method: Compositional matrix adjust. Identities = 212/341 (62%), Positives = 279/341 (81%), Gaps = 4/341 (1%) Query: 1 MIEIKNVYKTFERKGQSIEALRGVSLSIARGDIYGVIGYSGAGKSTLIRLVNALERPTAG 60 MIE++++ KTFERKG ++AL VSL++ GDI+G+IGYSGAGKSTL+R+VN+LE PT+G Sbjct: 1 MIELQHISKTFERKGVKLQALNNVSLTVEDGDIFGIIGYSGAGKSTLLRMVNSLESPTSG 60 Query: 61 EVLVEGQDLAGYNPAELRQAKKNIGMIFQHFNLLESKTVFDNIAIPLVLLKRSKQEIRER 120 +V+++G +L ++ +LR KKNIGMIFQ+FNLLESKTVF N+A+PL+LL R+K+ I++R Sbjct: 61 DVIIDGNNLHDFSHEQLRLLKKNIGMIFQNFNLLESKTVFKNVAMPLILLGRNKKFIQDR 120 Query: 121 VTELLKFTGLGDKANSYPKELSGGQKQRVGIARALASNPSILLCDEATSALDPQTTKSIL 180 V ELL F GLGDK+NS+P ELSGGQKQRVGIARALA+NPSILLCDEATS+LDPQTT IL Sbjct: 121 VAELLDFVGLGDKSNSFPNELSGGQKQRVGIARALATNPSILLCDEATSSLDPQTTAQIL 180 Query: 181 ELLQKINEEYNITIMIITHEMAVIQQICNKVAVMERGEIIEQGNVLDVFGSPHHPTTQSF 240 LL+KIN++YNIT+++ITHEM+VIQ+ICNKVAVME G+IIEQG+VL VFG P HPTT +F Sbjct: 181 LLLKKINQQYNITVLLITHEMSVIQKICNKVAVMENGQIIEQGSVLTVFGHPTHPTTLNF 240 Query: 241 VQTVIHNSVPQSVLHTLKAENGRRLFKLKFXXXXGA-AWEPIINSLIRRYEVDVNILFAN 299 V+TVI +S+P SV L + R F+L F GA A +P+IN LIR+Y++ VNILFAN Sbjct: 241 VRTVIKDSLPDSVRKLLDNSHAGRQFRLAF---MGAIATQPVINQLIRQYDIGVNILFAN 297 Query: 300 MTEIQNTTLGNMILQMHGDHAVIDRAADFITSQGVEITEVE 340 M+EIQ+TTLG+M+L + G++ +ID A + G++I E+ Sbjct: 298 MSEIQDTTLGHMVLLLTGNNNLIDSAVTHLAGTGIDIQEIH 338 >C4RXH1.1 C4RXH1_YERBE Methionine import ATP-binding protein metN 2 Length = 338 Score = 442 bits (1137), Expect = e-122, Method: Compositional matrix adjust. Identities = 209/339 (61%), Positives = 280/339 (82%), Gaps = 2/339 (0%) Query: 1 MIEIKNVYKTFERKGQSIEALRGVSLSIARGDIYGVIGYSGAGKSTLIRLVNALERPTAG 60 MIE++++ KTFER+G ++AL VSL++A GDI+G+IGYSGAGKSTL+R+VN+LE PT+G Sbjct: 1 MIELQHISKTFERQGIKLQALNNVSLTVADGDIFGIIGYSGAGKSTLLRMVNSLESPTSG 60 Query: 61 EVLVEGQDLAGYNPAELRQAKKNIGMIFQHFNLLESKTVFDNIAIPLVLLKRSKQEIRER 120 V+++G++L ++ +LR KKNIGMIFQ+FNLLESKTVF N+A+PL+LL ++K+ I+ER Sbjct: 61 NVIIDGKNLHDFSHEQLRLLKKNIGMIFQNFNLLESKTVFKNVAMPLILLGKNKKFIQER 120 Query: 121 VTELLKFTGLGDKANSYPKELSGGQKQRVGIARALASNPSILLCDEATSALDPQTTKSIL 180 V ELL F GLGDK++S+P ELSGGQKQRVGIARALA+NPSILLCDEATS+LDPQTT IL Sbjct: 121 VAELLDFVGLGDKSHSFPNELSGGQKQRVGIARALATNPSILLCDEATSSLDPQTTAQIL 180 Query: 181 ELLQKINEEYNITIMIITHEMAVIQQICNKVAVMERGEIIEQGNVLDVFGSPHHPTTQSF 240 LL+KIN++YNIT+++ITHEM+VIQ+ICNKVAVME G+IIEQG+VL VFG P HPTT +F Sbjct: 181 LLLKKINQQYNITVLLITHEMSVIQKICNKVAVMENGQIIEQGSVLTVFGHPTHPTTLNF 240 Query: 241 VQTVIHNSVPQSVLHTLKAENGRRLFKLKFXXXXGAAWEPIINSLIRRYEVDVNILFANM 300 V+TVI +S+P+SV L + R F+L F A +P+IN LI++Y++ VNILFANM Sbjct: 241 VRTVIKDSLPESVKKLLNNSHAGRQFRLAF--MGSIATQPVINQLIKQYDIGVNILFANM 298 Query: 301 TEIQNTTLGNMILQMHGDHAVIDRAADFITSQGVEITEV 339 +EIQ+TTLG+M+L + G++ ID A ++T G++I E+ Sbjct: 299 SEIQDTTLGHMVLLLTGENHTIDGAVSYLTETGIDIQEI 337 >A1JT91.1 A1JT91_YERE8 Putative ABC transport family protein Length = 338 Score = 439 bits (1128), Expect = e-121, Method: Compositional matrix adjust. Identities = 210/340 (61%), Positives = 280/340 (82%), Gaps = 4/340 (1%) Query: 1 MIEIKNVYKTFERKGQSIEALRGVSLSIARGDIYGVIGYSGAGKSTLIRLVNALERPTAG 60 MIE++++ KTFERKG ++AL V+L++ GDI+G+IGYSGAGKSTL+R+VN+LE PTAG Sbjct: 1 MIELQHISKTFERKGIKLQALNDVNLTVEDGDIFGIIGYSGAGKSTLLRMVNSLESPTAG 60 Query: 61 EVLVEGQDLAGYNPAELRQAKKNIGMIFQHFNLLESKTVFDNIAIPLVLLKRSKQEIRER 120 V+++G++L ++ +LR KKNIGMIFQ+FNLLESKTVF N+A+PL+LL ++K+ I+ R Sbjct: 61 NVIIDGKNLHDFSQEQLRLLKKNIGMIFQNFNLLESKTVFKNVAMPLILLGKNKKFIQVR 120 Query: 121 VTELLKFTGLGDKANSYPKELSGGQKQRVGIARALASNPSILLCDEATSALDPQTTKSIL 180 V ELL F GLGDK++S+P ELSGGQKQRVGIARALA+NPSILLCDEATS+LDPQTT IL Sbjct: 121 VAELLDFVGLGDKSHSFPNELSGGQKQRVGIARALATNPSILLCDEATSSLDPQTTAQIL 180 Query: 181 ELLQKINEEYNITIMIITHEMAVIQQICNKVAVMERGEIIEQGNVLDVFGSPHHPTTQSF 240 LL+KIN++YNIT+++ITHEM+VIQ+ICNKVAVME G+IIEQG+VL VFG P HPTT +F Sbjct: 181 LLLKKINQQYNITVLLITHEMSVIQKICNKVAVMENGQIIEQGSVLTVFGHPTHPTTINF 240 Query: 241 VQTVIHNSVPQSVLHTLKAENGRRLFKLKFXXXXGA-AWEPIINSLIRRYEVDVNILFAN 299 V+TVI +S+P+SV L + R F+L F GA A +P+IN LIR+Y++ VNILFAN Sbjct: 241 VRTVIKDSLPESVKKLLNNSHTGRQFRLAF---VGAIATQPVINQLIRKYDIGVNILFAN 297 Query: 300 MTEIQNTTLGNMILQMHGDHAVIDRAADFITSQGVEITEV 339 M+EIQ+TTLG+M+L + G++ ID A +++ G++I E+ Sbjct: 298 MSEIQDTTLGHMVLLLTGENHTIDSAVNYLAGTGIDIQEI 337 >C4SE98.1 C4SE98_YERMO Methionine import ATP-binding protein metN 2 Length = 338 Score = 437 bits (1124), Expect = e-121, Method: Compositional matrix adjust. Identities = 206/339 (60%), Positives = 278/339 (82%), Gaps = 2/339 (0%) Query: 1 MIEIKNVYKTFERKGQSIEALRGVSLSIARGDIYGVIGYSGAGKSTLIRLVNALERPTAG 60 MIE++++ KTFERKG ++AL VSL++ GDI+G+IGYSGAGKSTL+R+VN+LE PT+G Sbjct: 1 MIELQHISKTFERKGIKLQALNNVSLTVENGDIFGIIGYSGAGKSTLLRMVNSLESPTSG 60 Query: 61 EVLVEGQDLAGYNPAELRQAKKNIGMIFQHFNLLESKTVFDNIAIPLVLLKRSKQEIRER 120 V+++G++L ++ +LR KK+IGMIFQ+FNLLESKTV+ N+A+PL+LL ++K+ I+ER Sbjct: 61 NVIIDGKNLHDFSQEQLRLLKKDIGMIFQNFNLLESKTVYKNVAMPLILLGKNKKFIQER 120 Query: 121 VTELLKFTGLGDKANSYPKELSGGQKQRVGIARALASNPSILLCDEATSALDPQTTKSIL 180 V ELL F GLGDK++S+P ELSGGQKQRVGIARALA+NPSILLCDEATS+LDPQTT IL Sbjct: 121 VAELLDFVGLGDKSHSFPNELSGGQKQRVGIARALATNPSILLCDEATSSLDPQTTAQIL 180 Query: 181 ELLQKINEEYNITIMIITHEMAVIQQICNKVAVMERGEIIEQGNVLDVFGSPHHPTTQSF 240 LL+KIN++YNIT+++ITHEM+VIQ+ICNKVAVME G+IIEQG+VL VFG P HPTT +F Sbjct: 181 LLLKKINQQYNITVLLITHEMSVIQKICNKVAVMENGQIIEQGSVLTVFGHPTHPTTLNF 240 Query: 241 VQTVIHNSVPQSVLHTLKAENGRRLFKLKFXXXXGAAWEPIINSLIRRYEVDVNILFANM 300 V+T I +S+P+SV L + R F+L F A +P+IN LI++Y++ VNILFANM Sbjct: 241 VRTAIKDSLPESVKKLLNHSHAGRQFRLAF--MGSIATQPVINQLIKQYDIGVNILFANM 298 Query: 301 TEIQNTTLGNMILQMHGDHAVIDRAADFITSQGVEITEV 339 +EIQ+TTLG+M+L + G++ ID A ++T G++I E+ Sbjct: 299 SEIQDTTLGHMVLLLTGENHTIDDAISYLTGTGIDIQEI 337 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,819,509,400 Number of extensions: 71051469 Number of successful extensions: 543013 Number of sequences better than 1.0e-05: 100722 Number of HSP's gapped: 404288 Number of HSP's successfully gapped: 110584 Length of query: 341 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 205 Effective length of database: 1,779,438,163 Effective search space: 364784823415 Effective search space used: 364784823415 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)