BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0015.p (239 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7GIU7.1 MTNN_ANOFW 5'-methylthioadenosine/S-adenosylhomocystein... 223 9e-57 C1KVE1.1 C1KVE1_LISMC Putative 5'-methylthioadenosine/S-adenosyl... 221 4e-56 Q4EGP8.1 Q4EGP8_LISMO MTA/SAH nucleosidase 221 4e-56 B8DE17.1 MTNN_LISMH 5'-methylthioadenosine/S-adenosylhomocystein... 221 5e-56 Q71ZH6.1 MTNN_LISMF 5'-methylthioadenosine/S-adenosylhomocystein... 221 5e-56 >B7GIU7.1 MTNN_ANOFW 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase EC=3.2.2.9 Length = 232 Score = 223 bits (569), Expect = 9e-57, Method: Compositional matrix adjust. Identities = 116/224 (51%), Positives = 154/224 (68%), Gaps = 1/224 (0%) Query: 1 MSIAVIGAMEEEVAPLLPKLRDVRSLKAGGGKLYAGVLDGQEVVLLQSGIGKVNAAVTTT 60 M+IA+IGAMEEEVA L K+ + Y+G LDG VVLL+SGIGKVNAA++TT Sbjct: 1 MNIAIIGAMEEEVAILREKIANRTETTVANCSFYSGTLDGANVVLLKSGIGKVNAAMSTT 60 Query: 61 LLLERFHCELIINTGAAGGLAADLKVGDVVIAEELVYSDVDATAFSYAFGQVPQMPQRYP 120 +LLERF +++INTG+AGG A L VGD+VI+ E+V+ DVD TAF YA+GQVP MP RY Sbjct: 61 ILLERFAPDVVINTGSAGGFAPSLNVGDIVISTEVVHHDVDVTAFGYAYGQVPGMPARY- 119 Query: 121 VAAGLLAQARKLVRRGVRQEQIVTGLITTADSFISDPERAVSIVSRFPEAKATDMEGAAI 180 A L QA + +R Q+ GLI T DSF+ DP R + ++FP+ A +ME AAI Sbjct: 120 AADERLIQAAETSAAHIRDIQVAKGLIATGDSFMHDPARVDFVRTQFPDLYAVEMEAAAI 179 Query: 181 AQTAYQFGVPFLAVRAISDIAGSDAAGLFKSHLELAAANSAEVV 224 AQ +QF VPF+ +RA+SDIAG ++ F+ L+ AA +S+E+V Sbjct: 180 AQVCHQFNVPFVVIRALSDIAGKESNVSFEQFLQKAALHSSELV 223 >C1KVE1.1 C1KVE1_LISMC Putative 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Length = 233 Score = 221 bits (563), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 116/231 (50%), Positives = 153/231 (66%), Gaps = 2/231 (0%) Query: 1 MSIAVIGAMEEEVAPLLPKLRDVRSLKAGGGKLYAGVLDGQEVVLLQSGIGKVNAAVTTT 60 M+I +IGAMEEEV L + V + GG K Y G + G+EVVLL+SGIGKVNAA+ TT Sbjct: 1 MTIGIIGAMEEEVELLKNSMPSVEEIVIGGAKFYVGEIAGKEVVLLESGIGKVNAALGTT 60 Query: 61 LLLERFHCELIINTGAAGGLAADLKVGDVVIAEELVYSDVDATAFSYAFGQVPQMPQRYP 120 L+ +RF E+IINTG+AGG+A L VGDV+I++ L Y DVD T F Y +GQVP+MP Y Sbjct: 61 LMADRFKPEVIINTGSAGGMAEGLAVGDVIISDRLAYGDVDVTEFGYTYGQVPRMPAFYQ 120 Query: 121 VAAGLLAQARKLVRR--GVRQEQIVTGLITTADSFISDPERAVSIVSRFPEAKATDMEGA 178 A LL +A + R + + V GL+ T DSFI P++ +I + FP+ KA +ME A Sbjct: 121 GDAVLLKKAETIYREYFAASENKAVYGLVVTNDSFIMRPDQHETIRTFFPDVKAVEMEAA 180 Query: 179 AIAQTAYQFGVPFLAVRAISDIAGSDAAGLFKSHLELAAANSAEVVLEILS 229 AIAQ AYQF +PFL +RAISD+A +A F + LAA SA ++E+L Sbjct: 181 AIAQVAYQFDIPFLIIRAISDLANQEATISFDEFIHLAAKQSATCIIELLK 231 >Q4EGP8.1 Q4EGP8_LISMO MTA/SAH nucleosidase Length = 233 Score = 221 bits (563), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 116/231 (50%), Positives = 153/231 (66%), Gaps = 2/231 (0%) Query: 1 MSIAVIGAMEEEVAPLLPKLRDVRSLKAGGGKLYAGVLDGQEVVLLQSGIGKVNAAVTTT 60 M+I +IGAMEEEV L + V + GG K Y G + G+EVVLL+SGIGKVNAA+ TT Sbjct: 1 MTIGIIGAMEEEVELLKNSMPSVEEIVIGGAKFYVGEIAGKEVVLLESGIGKVNAALGTT 60 Query: 61 LLLERFHCELIINTGAAGGLAADLKVGDVVIAEELVYSDVDATAFSYAFGQVPQMPQRYP 120 L+ +RF E+IINTG+AGG+A L VGDV+I++ L Y DVD T F Y +GQVP+MP Y Sbjct: 61 LMADRFKPEVIINTGSAGGMAEGLAVGDVIISDRLAYGDVDVTEFGYTYGQVPRMPAFYQ 120 Query: 121 VAAGLLAQARKLVRR--GVRQEQIVTGLITTADSFISDPERAVSIVSRFPEAKATDMEGA 178 A LL +A + R + + V GL+ T DSFI P++ +I + FP+ KA +ME A Sbjct: 121 GDAVLLKKAETIYREYFAASENKAVYGLVVTNDSFIMRPDQHETIRTFFPDVKAVEMEAA 180 Query: 179 AIAQTAYQFGVPFLAVRAISDIAGSDAAGLFKSHLELAAANSAEVVLEILS 229 AIAQ AYQF +PFL +RAISD+A +A F + LAA SA ++E+L Sbjct: 181 AIAQVAYQFDIPFLIIRAISDLANQEATISFDEFIHLAAKQSATCIIELLK 231 >B8DE17.1 MTNN_LISMH 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase EC=3.2.2.9 Length = 233 Score = 221 bits (562), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 116/231 (50%), Positives = 153/231 (66%), Gaps = 2/231 (0%) Query: 1 MSIAVIGAMEEEVAPLLPKLRDVRSLKAGGGKLYAGVLDGQEVVLLQSGIGKVNAAVTTT 60 M+I +IGAMEEEV L + V + GG K Y G + G+EVVLL+SGIGKVNAA+ TT Sbjct: 1 MTIGIIGAMEEEVELLKNSMPSVEEIVIGGAKFYVGEVAGKEVVLLESGIGKVNAALGTT 60 Query: 61 LLLERFHCELIINTGAAGGLAADLKVGDVVIAEELVYSDVDATAFSYAFGQVPQMPQRYP 120 L+ +RF E+IINTG+AGG+A L VGDV+I++ L Y DVD T F Y +GQVP+MP Y Sbjct: 61 LMADRFKPEVIINTGSAGGMAEGLAVGDVIISDRLAYGDVDVTEFGYTYGQVPRMPAFYQ 120 Query: 121 VAAGLLAQARKLVRR--GVRQEQIVTGLITTADSFISDPERAVSIVSRFPEAKATDMEGA 178 A LL +A + R + + V GL+ T DSFI P++ +I + FP+ KA +ME A Sbjct: 121 GDAVLLKKAETIYREYFAASENKAVYGLVVTNDSFIMRPDQHETIRTFFPDVKAVEMEAA 180 Query: 179 AIAQTAYQFGVPFLAVRAISDIAGSDAAGLFKSHLELAAANSAEVVLEILS 229 AIAQ AYQF +PFL +RAISD+A +A F + LAA SA ++E+L Sbjct: 181 AIAQVAYQFDIPFLIIRAISDLANQEATISFDEFIHLAAKQSATCIIELLK 231 >Q71ZH6.1 MTNN_LISMF 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase EC=3.2.2.9 Length = 233 Score = 221 bits (562), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 116/231 (50%), Positives = 153/231 (66%), Gaps = 2/231 (0%) Query: 1 MSIAVIGAMEEEVAPLLPKLRDVRSLKAGGGKLYAGVLDGQEVVLLQSGIGKVNAAVTTT 60 M+I +IGAMEEEV L + V + GG K Y G + G+EVVLL+SGIGKVNAA+ TT Sbjct: 1 MTIGIIGAMEEEVELLKNSMPSVEEIVIGGAKFYVGEIAGKEVVLLESGIGKVNAALGTT 60 Query: 61 LLLERFHCELIINTGAAGGLAADLKVGDVVIAEELVYSDVDATAFSYAFGQVPQMPQRYP 120 L+ +RF E+IINTG+AGG+A L VGDV+I++ L Y DVD T F Y +GQVP+MP Y Sbjct: 61 LMADRFKPEVIINTGSAGGMAEGLAVGDVIISDRLAYGDVDVTEFGYTYGQVPRMPAFYQ 120 Query: 121 VAAGLLAQARKLVRR--GVRQEQIVTGLITTADSFISDPERAVSIVSRFPEAKATDMEGA 178 A LL +A + R + + V GL+ T DSFI P++ +I + FP+ KA +ME A Sbjct: 121 GDAVLLKKAETIYREYFAASENKAVYGLVVTNDSFIMRPDQHETIRTFFPDVKAVEMEAA 180 Query: 179 AIAQTAYQFGVPFLAVRAISDIAGSDAAGLFKSHLELAAANSAEVVLEILS 229 AIAQ AYQF +PFL +RAISD+A +A F + LAA SA ++E+L Sbjct: 181 AIAQVAYQFDIPFLIIRAISDLANQEATISFDEFIHLAARQSATCIIELLK 231 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,265,302,536 Number of extensions: 48365047 Number of successful extensions: 146474 Number of sequences better than 1.0e-05: 1235 Number of HSP's gapped: 145469 Number of HSP's successfully gapped: 1238 Length of query: 239 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 107 Effective length of database: 1,817,122,223 Effective search space: 194432077861 Effective search space used: 194432077861 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)