BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0007.p (267 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAW4.1 B3KAW4_9BACI Cof-like hydrolase 422 e-116 B1DJJ7.1 B1DJJ7_9BACL Cof-like hydrolase 320 1e-85 Q3AFE4.1 Q3AFE4_CARHZ HAD-superfamily hydrolase, subfamily IIB 199 2e-49 A5CYL3.1 A5CYL3_PELTS Predicted hydrolase 182 2e-44 A4J905.1 A4J905_DESRM Cof-like hydrolase 177 7e-43 >B3KAW4.1 B3KAW4_9BACI Cof-like hydrolase Length = 268 Score = 422 bits (1086), Expect = e-116, Method: Compositional matrix adjust. Identities = 205/262 (78%), Positives = 220/262 (83%) Query: 2 YKLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNVP 61 YKL+AIDIDDTLINDDKEVTP Q ALEQ RAYASAQAIARQTGLNVP Sbjct: 4 YKLMAIDIDDTLINDDKEVTPGVQQALEQAVAHGVVVTLATGRAYASAQAIARQTGLNVP 63 Query: 62 IITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYIDDKLYARDENQKLV 121 IITYQGAL+KNL+DEKVLYER+VP DA KLF YCVEHNLHLQTYIDDKLYAR+ENQKL+ Sbjct: 64 IITYQGALIKNLLDEKVLYERFVPADAAVKLFEYCVEHNLHLQTYIDDKLYAREENQKLI 123 Query: 122 DYATLNGTKYYIEPDWEKLVPQKTPKMLIIDDPDFLDELSPILRELLGDSVHITKSKPHF 181 DY LN T YYIEPD+ +V + TPKMLIIDDPD+LDEL PI RELLGD VHITKSKPHF Sbjct: 124 DYCALNRTPYYIEPDFMTMVQKPTPKMLIIDDPDYLDELIPIFRELLGDEVHITKSKPHF 183 Query: 182 LEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANAIPALKDI 241 LE MHKEGTKG AL FLA HFGC+L ETIAVGDSWNDHEMLEAAGLGVAM N+I LK I Sbjct: 184 LEFMHKEGTKGHALTFLADHFGCDLKETIAVGDSWNDHEMLEAAGLGVAMGNSIEPLKAI 243 Query: 242 ADFVTLSNNEDGVKYAIDKFIL 263 AD+VTL NNEDGVK+ IDKF+L Sbjct: 244 ADYVTLGNNEDGVKHVIDKFVL 265 >B1DJJ7.1 B1DJJ7_9BACL Cof-like hydrolase Length = 268 Score = 320 bits (819), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 153/266 (57%), Positives = 196/266 (73%) Query: 1 MYKLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNV 60 MYKLIAID+DDTL+NDD VT T+ A+E R YASA+ IA Q LNV Sbjct: 1 MYKLIAIDVDDTLLNDDLIVTEGTKKAMEDAIALGVTVTLATGRMYASAKKIADQIQLNV 60 Query: 61 PIITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYIDDKLYARDENQKL 120 PIITYQG+LVK L+D +VLYER VPQDA ++L+ + LHLQ Y DD LY ++ N+K Sbjct: 61 PIITYQGSLVKTLLDGEVLYERCVPQDAAKELYAITEQRGLHLQLYADDILYVKEANEKA 120 Query: 121 VDYATLNGTKYYIEPDWEKLVPQKTPKMLIIDDPDFLDELSPILRELLGDSVHITKSKPH 180 + Y+TL Y + D+E L+ + KMLIIDDP +LD+L+ L+ ++GD VHITKSKPH Sbjct: 121 IKYSTLTNIPYVVAEDFEALLDKPNNKMLIIDDPAYLDQLAEELKPIIGDRVHITKSKPH 180 Query: 181 FLEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANAIPALKD 240 +LE MHKEGTKG AL F+A H GC + +TIA+GD+WNDHEM+EAAGLGVAM NA P LK+ Sbjct: 181 YLEFMHKEGTKGHALRFMAEHLGCGVEQTIAIGDAWNDHEMIEAAGLGVAMGNATPKLKE 240 Query: 241 IADFVTLSNNEDGVKYAIDKFILQTA 266 IAD++TL+NNE+GV++ I+KF+LQ A Sbjct: 241 IADYITLTNNEEGVRHVIEKFVLQPA 266 >Q3AFE4.1 Q3AFE4_CARHZ HAD-superfamily hydrolase, subfamily IIB Length = 266 Score = 199 bits (506), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 108/265 (40%), Positives = 159/265 (60%), Gaps = 2/265 (0%) Query: 2 YKLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNVP 61 Y+L+A+D+DDT +N + +V+P Q A+ + R Y SA+ A ++P Sbjct: 3 YRLLAVDLDDTFLNKELQVSPRVQQAVIEALKKGIIVTLATGRMYRSAKKYAFSFLGDIP 62 Query: 62 IITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYIDDKLYARDENQKLV 121 +ITY GAL+K E+ +Y + VP + ++ H HL Y DD+L ++NQ + Sbjct: 63 LITYNGALIKYSRSEREVYHQPVPGELALTIYRRVKGH-FHLNVYQDDELLVEEDNQYIR 121 Query: 122 DYATLNGTKYYIEPDWEKLVPQKTP-KMLIIDDPDFLDELSPILRELLGDSVHITKSKPH 180 DY+ + G + + + E+L+ +K P K+L I DP+ LD+L +HITKSKPH Sbjct: 122 DYSKIAGVPFRVVSNIEELLGKKAPTKLLAIGDPEELDQLWEETNGEFRGVLHITKSKPH 181 Query: 181 FLEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANAIPALKD 240 +LE + KG AL+ LA H G L+ET+AVGDS+ND EMLEAAGLGVAM NA+P +K Sbjct: 182 YLEFLAAGVNKGEALKILAQHLGISLTETVAVGDSYNDLEMLEAAGLGVAMGNALPEVKR 241 Query: 241 IADFVTLSNNEDGVKYAIDKFILQT 265 AD V +N+EDG+ Y I++ IL+ Sbjct: 242 RADLVVPANDEDGIAYLINEIILKN 266 >A5CYL3.1 A5CYL3_PELTS Predicted hydrolase Length = 274 Score = 182 bits (463), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 108/262 (41%), Positives = 151/262 (57%) Query: 2 YKLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNVP 61 +KL+A+D+DDTL++ D ++T + A+ R Y SA AR+ GLN+P Sbjct: 13 FKLVAVDMDDTLLSRDLKLTGRVREAVAAVRAAGVYFTISTGRMYRSALPFARELGLNIP 72 Query: 62 IITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYIDDKLYARDENQKLV 121 +ITYQGALVKN +++ R +P R++ E HL Y+DD+L + + Sbjct: 73 LITYQGALVKNSHTGELVLYRPLPLARAREIIARIHELGYHLNGYLDDRLLVERDTPEGR 132 Query: 122 DYATLNGTKYYIEPDWEKLVPQKTPKMLIIDDPDFLDELSPILRELLGDSVHITKSKPHF 181 YA ++G + I D + + + K+L I + LD LS L L VHI KSKPHF Sbjct: 133 RYAAMSGVEAEIVGDLLEYLDRDPIKVLTIAEEPLLDRLSAELTPLYRGKVHIVKSKPHF 192 Query: 182 LEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANAIPALKDI 241 LE H + TKG AL +LA FG + E +AVGDS+ND EMLE AGLGVA+ANA +K Sbjct: 193 LEFSHPQATKGDALAYLAGCFGVKREEVMAVGDSYNDLEMLEYAGLGVAVANAREDVKKK 252 Query: 242 ADFVTLSNNEDGVKYAIDKFIL 263 A +VT + DGV A++KF+L Sbjct: 253 AGYVTSAAYGDGVVEALEKFVL 274 >A4J905.1 A4J905_DESRM Cof-like hydrolase Length = 268 Score = 177 bits (450), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 101/262 (38%), Positives = 144/262 (54%) Query: 2 YKLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNVP 61 YKL+AID+DDTL+N +++ + +++ R Y SA A++ LN+P Sbjct: 5 YKLLAIDLDDTLLNGQLKISSRNKEYIKRAQEAGIHVTLATGRMYCSALRYAKELELNLP 64 Query: 62 IITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYIDDKLYARDENQKLV 121 +ITYQGALVK +VL R VP R++ + H+ Y+DD LY + Sbjct: 65 LITYQGALVKEAQTGEVLLHRPVPLVLAREVIQRGSQLGYHINVYVDDTLYVEKITPEAE 124 Query: 122 DYATLNGTKYYIEPDWEKLVPQKTPKMLIIDDPDFLDELSPILRELLGDSVHITKSKPHF 181 Y ++G + + + + K+L++ + LD L +R G S+HI KSKPHF Sbjct: 125 VYQRISGITAHPVGNLLDFLQEDPTKVLMVGGIEELDRLGEEMRCQYGTSLHICKSKPHF 184 Query: 182 LEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANAIPALKDI 241 LE H + TKGLAL+ LA +G + IA+GD+ ND EM++ AGLGV M N P +K Sbjct: 185 LEFSHPQATKGLALDTLAKGWGLTAEQVIAIGDAPNDLEMIDYAGLGVVMGNGEPEVKAK 244 Query: 242 ADFVTLSNNEDGVKYAIDKFIL 263 AD+VT SN EDGV I KFI Sbjct: 245 ADYVTHSNEEDGVAEVIAKFIF 266 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,512,920,855 Number of extensions: 61406873 Number of successful extensions: 175971 Number of sequences better than 1.0e-05: 3565 Number of HSP's gapped: 172995 Number of HSP's successfully gapped: 3567 Length of query: 267 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 134 Effective length of database: 1,807,701,208 Effective search space: 242231961872 Effective search space used: 242231961872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)