BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0003.p (336 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAW7.1 B3KAW7_9BACI Lipoyltransferase and lipoate-protein ligase 535 e-150 C5D262.1 C5D262_9BACI Lipoyltransferase and lipoate-protein ligase 422 e-116 A6CQM8.1 A6CQM8_9BACI YhfJ 413 e-113 Q2B2C0.1 Q2B2C0_9BACI YhfJ 411 e-113 C1P8H4.1 C1P8H4_BACCO Lipoyltransferase and lipoate-protein liga... 408 e-112 >B3KAW7.1 B3KAW7_9BACI Lipoyltransferase and lipoate-protein ligase Length = 333 Score = 535 bits (1379), Expect = e-150, Method: Compositional matrix adjust. Identities = 262/336 (77%), Positives = 287/336 (85%), Gaps = 3/336 (0%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMDDSYLLFYINSPSIIIGKHQNTIEEINQEYVK 60 MLFIDN GI D SINLAIEE+ALK+L MD+SYLLFYIN PSIIIGKHQNTIEEINQEYV+ Sbjct: 1 MLFIDNQGIHDPSINLAIEEYALKHLSMDESYLLFYINKPSIIIGKHQNTIEEINQEYVR 60 Query: 61 DHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAELS 120 D+NIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVID+LQ MGV AELS Sbjct: 61 DNNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDFLQKMGVPAELS 120 Query: 121 GRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXXX 180 GRND QVGE+KISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLN NPE Sbjct: 121 GRNDL---QVGEKKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNANPEKFKSKSTKS 177 Query: 181 XXXXXANIKDLLGKEMTIEEFRAGLLRSIFGMEPSEVPQYKLTMDDWVRINEISREHYQN 240 ANIK+LLG +MTIE+FRA LLRSIFGMEP +VPQY L DW IN+IS+E YQN Sbjct: 178 VRSRVANIKELLGTDMTIEQFRAELLRSIFGMEPDQVPQYVLQEKDWEIINQISKERYQN 237 Query: 241 WDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRGK 300 WDWNYGLSP+SNVKH RKFP G++DIRMDI + +I +IKIYGDFFGVGDVAD+EN LRGK Sbjct: 238 WDWNYGLSPESNVKHARKFPVGIIDIRMDIREGHIGEIKIYGDFFGVGDVADIENLLRGK 297 Query: 301 RYENAEVREALAGLDLKHYFGRIEPEDFIGLIFLEE 336 RYE VR AL+G+D+KHYFG +E +D IGLIFLEE Sbjct: 298 RYEETAVRTALSGIDVKHYFGNLELDDLIGLIFLEE 333 >C5D262.1 C5D262_9BACI Lipoyltransferase and lipoate-protein ligase Length = 329 Score = 422 bits (1086), Expect = e-116, Method: Compositional matrix adjust. Identities = 204/333 (61%), Positives = 259/333 (77%), Gaps = 4/333 (1%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMDDSYLLFYINSPSIIIGKHQNTIEEINQEYVK 60 MLFIDN GITD INLAIEE+ALKNL ++++YLLFYIN PSIIIGK+QNTIEEIN EYV+ Sbjct: 1 MLFIDNKGITDPRINLAIEEYALKNLDINETYLLFYINEPSIIIGKNQNTIEEINTEYVE 60 Query: 61 DHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAELS 120 + I VVRRLSGGGAVYHDLGNLNFSFITKDDG SF NF KFT+P+I L+ +GVNAELS Sbjct: 61 KNGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFLNFRKFTEPIIKALKKLGVNAELS 120 Query: 121 GRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXXX 180 GRND V +KISGNAQFSTRGRMFSHGTL+FD ++++ ++LNV + Sbjct: 121 GRNDII---VEGRKISGNAQFSTRGRMFSHGTLLFDSKIENIVSALNVKKDKIESKGIKS 177 Query: 181 XXXXXANIKDLLGKEMTIEEFRAGLLRSIFGMEPSEVPQYKLTMDDWVRINEISREHYQN 240 ANI + L ++MTIEEFR+ LL SIFG + ++P+Y LT +DW I+++SRE YQN Sbjct: 178 IRSRVANISEFLKEKMTIEEFRSVLLESIFGKD-KDIPEYVLTEEDWANIHKLSRERYQN 236 Query: 241 WDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRGK 300 W+WNYG SPK N++H+ +FPAG +D+R++++ I + KIYGDFFG GDV+++E L GK Sbjct: 237 WEWNYGKSPKFNLQHSHRFPAGQIDVRLEVQKGIIENCKIYGDFFGAGDVSEIEEKLIGK 296 Query: 301 RYENAEVREALAGLDLKHYFGRIEPEDFIGLIF 333 RYE +E+ +AL +D+KHYFG IE E+FI LI+ Sbjct: 297 RYEKSEIEKALQDVDIKHYFGNIEKEEFINLIY 329 >A6CQM8.1 A6CQM8_9BACI YhfJ Length = 328 Score = 413 bits (1061), Expect = e-113, Method: Compositional matrix adjust. Identities = 200/333 (60%), Positives = 253/333 (75%), Gaps = 5/333 (1%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMDDSYLLFYINSPSIIIGKHQNTIEEINQEYVK 60 M FIDN GITD INLAIEE+ALKNL +++ YLLFYIN PSIIIGK+QNTIEEIN EYV+ Sbjct: 1 MKFIDNKGITDPRINLAIEEYALKNLDINEDYLLFYINEPSIIIGKNQNTIEEINTEYVE 60 Query: 61 DHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAELS 120 N+ VVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNF KFT+PVI L+ +GVNAELS Sbjct: 61 AQNLHVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFQKFTEPVIKALKKLGVNAELS 120 Query: 121 GRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXXX 180 GRND + +KISGNAQFST+GRMFSHGTL+FD +++V ++L V + Sbjct: 121 GRND---IEAEGRKISGNAQFSTKGRMFSHGTLLFDSEIENVVSALKVRKDKIESKGIKS 177 Query: 181 XXXXXANIKDLLGKEMTIEEFRAGLLRSIFGMEPSEVPQYKLTMDDWVRINEISREHYQN 240 ANI + L ++MTI +FR+ LLR IF + +EV +Y LT +DW INE+S E YQN Sbjct: 178 IRSRVANISEFLEEKMTITDFRSMLLRYIF--DENEVEEYVLTEEDWKNINELSEERYQN 235 Query: 241 WDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRGK 300 W WNYG SPK N++H+ +FP G +DIR+++ I + KI+GDFFGVGDV+D+EN L G Sbjct: 236 WKWNYGKSPKFNLQHSHRFPVGSIDIRLEVNKGIIENCKIFGDFFGVGDVSDIENKLTGL 295 Query: 301 RYENAEVREALAGLDLKHYFGRIEPEDFIGLIF 333 +YE AEV +AL G++++HYFG++ ED + L++ Sbjct: 296 KYEKAEVEKALEGVEIQHYFGKVTKEDIVNLVY 328 >Q2B2C0.1 Q2B2C0_9BACI YhfJ Length = 330 Score = 411 bits (1057), Expect = e-113, Method: Compositional matrix adjust. Identities = 201/334 (60%), Positives = 254/334 (76%), Gaps = 5/334 (1%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMD-DSYLLFYINSPSIIIGKHQNTIEEINQEYV 59 MLFIDN GITD INLAIEE+ALKNL +D +SYLLFYIN PSIIIGK+QNTIEEIN EYV Sbjct: 1 MLFIDNKGITDPRINLAIEEYALKNLDIDQESYLLFYINEPSIIIGKNQNTIEEINTEYV 60 Query: 60 KDHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAEL 119 + + I VVRRLSGGGAVYHDLGNLNFSFITKDDG SFHNF KFT+PV++ L+ MGV AEL Sbjct: 61 EKNGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFHNFRKFTEPVVEALKKMGVAAEL 120 Query: 120 SGRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXX 179 SGRND +KISGNAQF+TRGRMFSHGTL+FD +D V ++L V + Sbjct: 121 SGRNDLTAEG---RKISGNAQFATRGRMFSHGTLLFDSEIDSVVSALKVRKDKIESKGIK 177 Query: 180 XXXXXXANIKDLLGKEMTIEEFRAGLLRSIFGMEPSEVPQYKLTMDDWVRINEISREHYQ 239 ANI + L ++++ E+FR+ LL++IFG + E+P+Y LT +DW +I+++S+E YQ Sbjct: 178 SIRSRVANISEFLEEKVSTEQFRSTLLKNIFG-DLDEIPEYVLTEEDWEKIHQLSKERYQ 236 Query: 240 NWDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRG 299 NWDWNYG SPK N++H+ +FP G +D+R+D+ I + KI+GDFFGVGDVA +E L G Sbjct: 237 NWDWNYGKSPKFNLQHSHRFPVGQIDVRLDVNKGIIGNCKIFGDFFGVGDVAVIEEKLSG 296 Query: 300 KRYENAEVREALAGLDLKHYFGRIEPEDFIGLIF 333 RYE A + EALA +++KHYFG + EDFI L++ Sbjct: 297 LRYEKAAIEEALADINIKHYFGNVTKEDFINLVY 330 >C1P8H4.1 C1P8H4_BACCO Lipoyltransferase and lipoate-protein ligase EC=2.7.7.63 Length = 328 Score = 408 bits (1049), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/333 (59%), Positives = 246/333 (73%), Gaps = 5/333 (1%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMDDSYLLFYINSPSIIIGKHQNTIEEINQEYVK 60 MLFIDN GITD INLAIEE+ALKNL ++++YLLFYIN PSIIIG++QNTIEEIN +YV Sbjct: 1 MLFIDNQGITDPRINLAIEEYALKNLDINETYLLFYINQPSIIIGRNQNTIEEINTDYVD 60 Query: 61 DHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAELS 120 + + VVRRLSGGGAVYHDLGN+NFSFITKDDG+SF NF KFT PV+ L+ +GVNAELS Sbjct: 61 KNGVIVVRRLSGGGAVYHDLGNINFSFITKDDGESFSNFRKFTDPVVQALRKLGVNAELS 120 Query: 121 GRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXXX 180 GRND QVG++KISGNAQFST GRMFSHGTLM + LD V +L V E Sbjct: 121 GRND---IQVGDRKISGNAQFSTNGRMFSHGTLMLNSELDKVAQALKVKKEKIESKGIKS 177 Query: 181 XXXXXANIKDLLGKEMTIEEFRAGLLRSIFGMEPSEVPQYKLTMDDWVRINEISREHYQN 240 ANI + L +++ FR LL SIFG E+P+Y+LT DW +I +IS+E YQ Sbjct: 178 VRSRVANISEFLDHPISMSAFRQLLLESIFG--GKEIPEYQLTEKDWEKIRQISKERYQT 235 Query: 241 WDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRGK 300 W+WNYG SPK N++H+R+FP G VD+R+++ I + KIYGDFFG+G+V D+E L G Sbjct: 236 WEWNYGKSPKFNLRHSRRFPVGTVDVRLEVNKGIIENCKIYGDFFGIGEVKDIEEKLAGC 295 Query: 301 RYENAEVREALAGLDLKHYFGRIEPEDFIGLIF 333 RYE A + +AL G+D++HYFG I EDFI LI+ Sbjct: 296 RYERAGIEKALEGIDIRHYFGNITKEDFISLIY 328 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,928,432,818 Number of extensions: 79076302 Number of successful extensions: 198514 Number of sequences better than 1.0e-05: 867 Number of HSP's gapped: 196956 Number of HSP's successfully gapped: 867 Length of query: 336 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 200 Effective length of database: 1,779,438,163 Effective search space: 355887632600 Effective search space used: 355887632600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)