BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0038.p (600 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5F9.1 B3K5F9_9BACI Glycoside hydrolase family 3 domain protein 686 0.0 Q9RL29.1 Q9RL29_STRCO Beta-glucosidase (EC 3.2.1.21) 530 e-148 C1L0B8.1 C1L0B8_LISMC Putative beta-glucosidase 496 e-138 Q4EIU8.1 Q4EIU8_LISMO Beta-glucosidase EC=3.2.1.21 496 e-138 Q71VX9.1 Q71VX9_LISMF Beta-glucosidase EC=3.2.1.21 496 e-138 >B3K5F9.1 B3K5F9_9BACI Glycoside hydrolase family 3 domain protein Length = 774 Score = 686 bits (1771), Expect = 0.0, Method: Compositional matrix adjust. Identities = 345/541 (63%), Positives = 401/541 (74%), Gaps = 5/541 (0%) Query: 1 MEVYKNSSFRVEERVQDLLSRMTLREKIGQLNQRMYGWDAYAWENG---ELVLTEAFRQE 57 ME Y+N++ V+ERV+DLLSRMTL+EK+GQLNQ +YGW AY G E LT+ F++ Sbjct: 1 MEKYRNAALPVDERVEDLLSRMTLQEKVGQLNQHLYGWMAYRRSEGTETEFELTDLFKEH 60 Query: 58 VXXXXXXXXLYGLFRSDPWSGVDYSNGITAADSASAANKVQRYIREHTRLGIPVLLSEEC 117 V LYGLFR+DPWSGV Y NGI D+A AN +QRY+ EH+RLGIPVLLSEEC Sbjct: 61 VEWGAGMGALYGLFRADPWSGVTYENGIPTQDNAKVANAIQRYVIEHSRLGIPVLLSEEC 120 Query: 118 PHGHQALDGTLLPVNLAVGATWNPQLMEQAYSKVALELRSRGAQIGLLSALDILHDPRWG 177 PHGHQALDGTLLPVN A+G+TWNP+L+E+AYS VA E+RSRGA +GL+S LDIL DPRWG Sbjct: 121 PHGHQALDGTLLPVNTAIGSTWNPELIEKAYSHVASEIRSRGAHLGLVSVLDILQDPRWG 180 Query: 178 RSEECYSEDPFLXXXXXXXXVRGMQGTRGEP-ASPGKVAVILKHLCXXXXXXXXXXXXXX 236 RSEECY EDP+L V GMQG E SP K A +LKHLC Sbjct: 181 RSEECYGEDPYLAARMTEAVVFGMQGRNPEEWKSPDKAAAVLKHLCAQGAGQGGRNAGPV 240 Query: 237 XIGERELREIHLPXXXXXXXXXXXXXXXXYNEIDGIPCHANEALLSGILRGEWGFDGIVM 296 GERELREIHLP YNE+DGIPCHAN+ LL+GILR EWGFDGIVM Sbjct: 241 PAGERELREIHLPAAEAGIKAGAAGVMAAYNELDGIPCHANDKLLTGILRDEWGFDGIVM 300 Query: 297 ADGQAIDRLVALTGSHEGAAALALSAGVDLSLWDKGFTTLEEAVKLGLASEADIDRAAAR 356 ADG AIDRLV++TG +E AAALALS+GVDLSLWDK FTTLEEAVK G A IDRA R Sbjct: 301 ADGTAIDRLVSITGDYESAAALALSSGVDLSLWDKSFTTLEEAVKQGKADMESIDRAVGR 360 Query: 357 VLTLKFRLGLFDEPYTDEQQALTAVGHADTRELNLQVARESVVLLKNEGGLLPLAAQH-R 415 VL LKFRLGLF++PY DE A + V +A RELN+ VARE+VVLLKN+ G+LPL + Sbjct: 361 VLGLKFRLGLFEQPYVDEGLAASTVNNAAARELNVSVAREAVVLLKNDKGVLPLNHNSIK 420 Query: 416 RIAVIGPNADRLYNQLGDYTSIQREGTGTTVLKGIRQCAPGGAEVVYALGCGIRESSTAG 475 R+AVIGPNADRLYNQLGDYTS+QREG+G T L+GIR AP G ++V+A G +R S G Sbjct: 421 RLAVIGPNADRLYNQLGDYTSVQREGSGVTALQGIRMAAPEGTDIVFAPGSSVRGMSAQG 480 Query: 476 LSEAVELARSADVAVLVLGGSSARQFGGDFEANGAAIISEGSPSEMDCGEGVDLADLRLG 535 L EA+E ARSAD VLVLGGSSARQFGG F+ NGAAI++EG P+EMDCGEGVDLA+LRLG Sbjct: 481 LPEAIEAARSADAVVLVLGGSSARQFGGMFDNNGAAIVAEGDPTEMDCGEGVDLAELRLG 540 Query: 536 G 536 G Sbjct: 541 G 541 >Q9RL29.1 Q9RL29_STRCO Beta-glucosidase (EC 3.2.1.21) Length = 762 Score = 530 bits (1365), Expect = e-148, Method: Compositional matrix adjust. Identities = 287/534 (53%), Positives = 346/534 (64%), Gaps = 10/534 (1%) Query: 1 MEVYKNSSFRVEERVQDLLSRMTLREKIGQLNQRMYGWDAYAWENGELVLTEAFRQEVXX 60 + +Y++ + V +RV+DLL RMTL EK+GQ+NQRMYGWDAY LT+AFR EV Sbjct: 3 LPLYRDPAAPVPDRVRDLLGRMTLAEKVGQVNQRMYGWDAYERAGDGHRLTDAFRAEVAA 62 Query: 61 XXXXXXLYGLFRSDPWSGVDYSNGITAADSASAANKVQRYIREHTRLGIPVLLSEECPHG 120 LYGL R+D WSGV +++G+ A D A A VQRY+ +HTRLGIPVLL EE PHG Sbjct: 63 FDGMGALYGLQRADAWSGVGFADGLDARDGARTAAAVQRYVMDHTRLGIPVLLVEEMPHG 122 Query: 121 HQALDGTLLPVNLAVGATWNPQLMEQAYSKVALELRSRGAQIGLLSALDILHDPRWGRSE 180 HQALDGT+LPVNLAVGATW+P L A + A ELR+RGA I L+SALD++ DPRWGRSE Sbjct: 123 HQALDGTVLPVNLAVGATWDPDLYADAVAGAAAELRARGAHIALVSALDLVRDPRWGRSE 182 Query: 181 ECYSEDPFLXXXXXXXXVRGMQGTRGEPASPGKVAVILKHLCXXXXXXXXXXXXXXXIGE 240 EC+SEDP+L V G A VAV+LKH +G Sbjct: 183 ECFSEDPYLAARMTEALVEG--------ARRAGVAVVLKHFAGQGATVGGRNSAATELGP 234 Query: 241 RELREIHLPXXXXXXXXXXXXXXXXYNEIDGIPCHANEALLSGILRGEWGFDGIVMADGQ 300 REL E+HL YNE DG+PC AN LL+ +LR EWGF+G+VMADG Sbjct: 235 RELHEVHLAAARAGVAAGAAGVMAAYNEFDGLPCVANRYLLTDLLRTEWGFEGVVMADGT 294 Query: 301 AIDRLVALTGSHEGAAALALSAGVDLSLWDKGFTTLEEAVKLGLASEADIDRAAARVLTL 360 A+DRLV LTG A ALAL AG DLSLWD FT L EAV+ GL SE+ +D A ARVLTL Sbjct: 295 AVDRLVRLTGDPVSAGALALDAGCDLSLWDASFTRLGEAVERGLVSESALDAAVARVLTL 354 Query: 361 KFRLGLFDEPYTDEQQALTAVGHADTRELNLQVARESVVLLKNEGGLLPLAAQHRRIAVI 420 KFRLGLF++P + V D EL ++AR SV LL +EGG+LPL+ RRIAV+ Sbjct: 355 KFRLGLFEQPLPPARS--ETVELPDPAELGERIARASVTLLAHEGGVLPLSRAVRRIAVL 412 Query: 421 GPNADRLYNQLGDYTSIQREGTGTTVLKGIRQCAPGGAEVVYALGCGIRESSTAGLSEAV 480 GPNAD + Q+GDYT+ QR G G TVL+GIR G EVV+ GC + +G+ AV Sbjct: 413 GPNADSVAQQIGDYTAPQRPGGGITVLEGIRAAVAAGTEVVHDRGCALVGDDVSGVPAAV 472 Query: 481 ELARSADVAVLVLGGSSARQFGGDFEANGAAIISEGSPSEMDCGEGVDLADLRL 534 LA +DVAVLVLGGSSAR F+ANGAA+ G+PS M CGEGVDLADL L Sbjct: 473 ALAAGSDVAVLVLGGSSARSPDTVFDANGAAVTGTGTPSGMTCGEGVDLADLAL 526 >C1L0B8.1 C1L0B8_LISMC Putative beta-glucosidase Length = 756 Score = 496 bits (1277), Expect = e-138, Method: Compositional matrix adjust. Identities = 261/534 (48%), Positives = 336/534 (62%), Gaps = 13/534 (2%) Query: 1 MEVYKNSSFRVEERVQDLLSRMTLREKIGQLNQRMYGWDAYAWENGELVLTEAFRQEVXX 60 M VY + V ERV+DLLS+MTL EK GQLNQRMYGW+A++ + +TE F++EV Sbjct: 1 MAVYLDKEKPVSERVEDLLSKMTLAEKCGQLNQRMYGWEAFSRDGETFHITEKFKEEVTR 60 Query: 61 XXXXXXLYGLFRSDPWSGVDYSNGITAADSASAANKVQRYIREHTRLGIPVLLSEECPHG 120 LYGLFR+DPWS ++ G++ ++ ANK+QRY+ E+TRLGIPVLL+EE PHG Sbjct: 61 FEGIGALYGLFRADPWSKMNKDTGVSRKNAGKVANKIQRYVIENTRLGIPVLLAEEVPHG 120 Query: 121 HQALDGTLLPVNLAVGATWNPQLMEQAYSKVALELRSRGAQIGLLSALDILHDPRWGRSE 180 HQALD PVNLA A++NP+L +Q S + E+ +G + L SALDIL DPRWGR+E Sbjct: 121 HQALDSESYPVNLARAASFNPELQKQVASAITEEISEKGVHLALASALDILRDPRWGRAE 180 Query: 181 ECYSEDPFLXXXXXXXXVRGMQGTRGEPASPGKVAVILKHLCXXXXXXXXXXXXXXXIGE 240 ECY EDP+L G Q + GK+AVILKH IG Sbjct: 181 ECYGEDPYLAAELTAAITEGFQAS-------GKIAVILKHFAAQGEPIGGHNSGPVSIGV 233 Query: 241 RELREIHLPXXXXXXXXXXXXXXXXYNEIDGIPCHANEALLSGILRGEWGFDGIVMADGQ 300 RELREI L YNEIDG+PCHAN+ LL+ ILR E GF GIVMADG Sbjct: 234 RELREIFLEPMRAGIRSGALGVMAAYNEIDGVPCHANKELLTTILREEMGFSGIVMADGC 293 Query: 301 AIDRLVALTGSHEGAAALALSAGVDLSLWDKGFTTLEEAVKLGLASEADIDRAAARVLTL 360 A+DRL+ L + AA +AL AGVDLSLWD+ F LEE+V+ G+ E +D A RVL + Sbjct: 294 ALDRLLKLNPDPKKAAKMALEAGVDLSLWDEVFPFLEESVEKGILDEKVVDDAVRRVLQV 353 Query: 361 KFRLGLFDEPYTDEQQALTAVGHADTRELNLQVARESVVLLKNEGGLLPLAAQHRRIAVI 420 KF+LGLF++P+ +E+ +D ++LNLQ ARE + LLKN+ LPL + ++IAV+ Sbjct: 354 KFQLGLFEQPFVEEE---IQAPKSDWKQLNLQAAREGICLLKNDFETLPLVGERKKIAVV 410 Query: 421 GPNADRLYNQLGDYTSIQREGTGTTVLKGIRQCAPGGAEVVYALGCGIRESSTAGLSEAV 480 GP+ D LYNQLGDYT+ Q E TVL+GI++ P EVV G IRE G+ A Sbjct: 411 GPSIDALYNQLGDYTAPQNESECVTVLEGIKKLLPKNWEVVSEKGTEIREELPDGIQRAE 470 Query: 481 ELARSADVAVLVLGGSSARQFGGDFEANGAAIISEGSPSEMDCGEGVDLADLRL 534 +A+ AD V+VLGGSSAR F +F NGA +S P+ MD GE VD+AD+ L Sbjct: 471 VVAKEADAIVMVLGGSSARNFDMEFLNNGA--VSSKGPN-MDAGENVDVADITL 521 >Q4EIU8.1 Q4EIU8_LISMO Beta-glucosidase EC=3.2.1.21 Length = 756 Score = 496 bits (1277), Expect = e-138, Method: Compositional matrix adjust. Identities = 261/534 (48%), Positives = 336/534 (62%), Gaps = 13/534 (2%) Query: 1 MEVYKNSSFRVEERVQDLLSRMTLREKIGQLNQRMYGWDAYAWENGELVLTEAFRQEVXX 60 M VY + V ERV+DLLS+MTL EK GQLNQRMYGW+A++ + +TE F++EV Sbjct: 1 MAVYLDKEKPVSERVEDLLSKMTLAEKCGQLNQRMYGWEAFSRDGETFHITEKFKEEVTR 60 Query: 61 XXXXXXLYGLFRSDPWSGVDYSNGITAADSASAANKVQRYIREHTRLGIPVLLSEECPHG 120 LYGLFR+DPWS ++ G++ ++ ANK+QRY+ E+TRLGIPVLL+EE PHG Sbjct: 61 FEGIGALYGLFRADPWSKMNKDTGVSRKNAGKVANKIQRYVIENTRLGIPVLLAEEVPHG 120 Query: 121 HQALDGTLLPVNLAVGATWNPQLMEQAYSKVALELRSRGAQIGLLSALDILHDPRWGRSE 180 HQALD PVNLA A++NP+L +Q S + E+ +G + L SALDIL DPRWGR+E Sbjct: 121 HQALDSESYPVNLARAASFNPELQKQVASAITEEISEKGVHLALASALDILRDPRWGRAE 180 Query: 181 ECYSEDPFLXXXXXXXXVRGMQGTRGEPASPGKVAVILKHLCXXXXXXXXXXXXXXXIGE 240 ECY EDP+L G Q + GK+AVILKH IG Sbjct: 181 ECYGEDPYLAAELTAAITEGFQAS-------GKIAVILKHFAAQGEPIGGHNSGPVSIGV 233 Query: 241 RELREIHLPXXXXXXXXXXXXXXXXYNEIDGIPCHANEALLSGILRGEWGFDGIVMADGQ 300 RELREI L YNEIDG+PCHAN+ LL+ ILR E GF GIVMADG Sbjct: 234 RELREIFLEPMRAGIRSGALGVMAAYNEIDGVPCHANKELLTTILREEMGFSGIVMADGC 293 Query: 301 AIDRLVALTGSHEGAAALALSAGVDLSLWDKGFTTLEEAVKLGLASEADIDRAAARVLTL 360 A+DRL+ L + AA +AL AGVDLSLWD+ F LEE+V+ G+ E +D A RVL + Sbjct: 294 ALDRLLKLNPDPKKAAKMALEAGVDLSLWDEVFPFLEESVEKGILDEKVVDDAVRRVLQV 353 Query: 361 KFRLGLFDEPYTDEQQALTAVGHADTRELNLQVARESVVLLKNEGGLLPLAAQHRRIAVI 420 KF+LGLF++P+ +E+ +D ++LNLQ ARE + LLKN+ LPL + ++IAV+ Sbjct: 354 KFQLGLFEQPFVEEE---IQAPKSDWKQLNLQAAREGICLLKNDFETLPLVGERKKIAVV 410 Query: 421 GPNADRLYNQLGDYTSIQREGTGTTVLKGIRQCAPGGAEVVYALGCGIRESSTAGLSEAV 480 GP+ D LYNQLGDYT+ Q E TVL+GI++ P EVV G IRE G+ A Sbjct: 411 GPSIDALYNQLGDYTAPQNESECVTVLEGIKKLLPKNWEVVSEKGTEIREELPDGIQRAE 470 Query: 481 ELARSADVAVLVLGGSSARQFGGDFEANGAAIISEGSPSEMDCGEGVDLADLRL 534 +A+ AD V+VLGGSSAR F +F NGA +S P+ MD GE VD+AD+ L Sbjct: 471 VVAKEADAIVMVLGGSSARNFDMEFLNNGA--VSSKGPN-MDAGENVDVADITL 521 >Q71VX9.1 Q71VX9_LISMF Beta-glucosidase EC=3.2.1.21 Length = 756 Score = 496 bits (1277), Expect = e-138, Method: Compositional matrix adjust. Identities = 261/534 (48%), Positives = 336/534 (62%), Gaps = 13/534 (2%) Query: 1 MEVYKNSSFRVEERVQDLLSRMTLREKIGQLNQRMYGWDAYAWENGELVLTEAFRQEVXX 60 M VY + V ERV+DLLS+MTL EK GQLNQRMYGW+A++ + +TE F++EV Sbjct: 1 MAVYLDKEKPVSERVEDLLSKMTLAEKCGQLNQRMYGWEAFSRDGETFHITEKFKEEVTR 60 Query: 61 XXXXXXLYGLFRSDPWSGVDYSNGITAADSASAANKVQRYIREHTRLGIPVLLSEECPHG 120 LYGLFR+DPWS ++ G++ ++ ANK+QRY+ E+TRLGIPVLL+EE PHG Sbjct: 61 FEGIGALYGLFRADPWSKMNKDTGVSRKNAGKVANKIQRYVIENTRLGIPVLLAEEVPHG 120 Query: 121 HQALDGTLLPVNLAVGATWNPQLMEQAYSKVALELRSRGAQIGLLSALDILHDPRWGRSE 180 HQALD PVNLA A++NP+L +Q S + E+ +G + L SALDIL DPRWGR+E Sbjct: 121 HQALDSESYPVNLARAASFNPELQKQVASAITEEISEKGVHLALASALDILRDPRWGRAE 180 Query: 181 ECYSEDPFLXXXXXXXXVRGMQGTRGEPASPGKVAVILKHLCXXXXXXXXXXXXXXXIGE 240 ECY EDP+L G Q + GK+AVILKH IG Sbjct: 181 ECYGEDPYLAAELTAAITEGFQAS-------GKIAVILKHFAAQGEPIGGHNSGPVSIGV 233 Query: 241 RELREIHLPXXXXXXXXXXXXXXXXYNEIDGIPCHANEALLSGILRGEWGFDGIVMADGQ 300 RELREI L YNEIDG+PCHAN+ LL+ ILR E GF GIVMADG Sbjct: 234 RELREIFLEPMRAGIRSGALGVMAAYNEIDGVPCHANKELLTTILREEMGFSGIVMADGC 293 Query: 301 AIDRLVALTGSHEGAAALALSAGVDLSLWDKGFTTLEEAVKLGLASEADIDRAAARVLTL 360 A+DRL+ L + AA +AL AGVDLSLWD+ F LEE+V+ G+ E +D A RVL + Sbjct: 294 ALDRLLKLNPDPKKAAKMALEAGVDLSLWDEVFPFLEESVEKGILDEKVVDDAVRRVLQV 353 Query: 361 KFRLGLFDEPYTDEQQALTAVGHADTRELNLQVARESVVLLKNEGGLLPLAAQHRRIAVI 420 KF+LGLF++P+ +E+ +D ++LNLQ ARE + LLKN+ LPL + ++IAV+ Sbjct: 354 KFQLGLFEQPFVEEE---IQAPKSDWKQLNLQAAREGICLLKNDFETLPLVGERKKIAVV 410 Query: 421 GPNADRLYNQLGDYTSIQREGTGTTVLKGIRQCAPGGAEVVYALGCGIRESSTAGLSEAV 480 GP+ D LYNQLGDYT+ Q E TVL+GI++ P EVV G IRE G+ A Sbjct: 411 GPSIDALYNQLGDYTAPQNESECVTVLEGIKKLLPKNWEVVSEKGTEIREELPDGIQRAE 470 Query: 481 ELARSADVAVLVLGGSSARQFGGDFEANGAAIISEGSPSEMDCGEGVDLADLRL 534 +A+ AD V+VLGGSSAR F +F NGA +S P+ MD GE VD+AD+ L Sbjct: 471 VVAKEADAIVMVLGGSSARNFDMEFLNNGA--VSSKGPN-MDAGENVDVADITL 521 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,032,752,321 Number of extensions: 112787693 Number of successful extensions: 298483 Number of sequences better than 1.0e-05: 1890 Number of HSP's gapped: 292493 Number of HSP's successfully gapped: 1948 Length of query: 600 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 458 Effective length of database: 1,722,912,073 Effective search space: 789093729434 Effective search space used: 789093729434 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)