BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0035.p (276 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5G2.1 B3K5G2_9BACI Binding-protein-dependent transport system... 406 e-111 Q8ES67.1 Q8ES67_OCEIH Sugar ABC transporter permease 328 4e-88 Q2BFH6.1 Q2BFH6_9BACI Sugar ABC transporter permease 312 2e-83 B8CXP3.1 B8CXP3_HALOH Binding-protein-dependent transport system... 296 2e-78 Q1AYD4.1 Q1AYD4_RUBXD Binding-protein-dependent transport system... 226 1e-57 >B3K5G2.1 B3K5G2_9BACI Binding-protein-dependent transport systems inner membrane component Length = 268 Score = 406 bits (1043), Expect = e-111, Method: Compositional matrix adjust. Identities = 201/268 (75%), Positives = 227/268 (84%) Query: 1 MKKKSPLFKIFAYTFLSLFAIMNIIPIFWMIVNSFKEEQEYAANPFSFPATLHFSNYTKA 60 M +++ F + AYTFL +FAI+NIIP FWMIV+SFK EQEYAANPFS P L FSNY KA Sbjct: 1 MYRQTKWFHLSAYTFLGIFAIINIIPFFWMIVDSFKTEQEYAANPFSIPTELQFSNYAKA 60 Query: 61 WEVANMNIYFLNXXXXXXXXXXXXXXXXXXAAYFLARFTFRLRGFTYALFLLGMLVPIHA 120 WEVANM+ YFLN AAYFLARF F+LRG TYA+FLLGM+VPIHA Sbjct: 61 WEVANMDTYFLNSIIVTVVSLVVTVLLGSLAAYFLARFNFKLRGATYAIFLLGMVVPIHA 120 Query: 121 TLIPIFLIMQKLSLIDTYLSLILPYTAFHLSLTVFILEGFMRGFPKDLEESGIMDGAGVF 180 TLIPIFLIM+ L L+DT+LSLILPYTAFHLSLT+FILEGFMRGFPKDLEESG+MDGAGVF Sbjct: 121 TLIPIFLIMRNLHLLDTHLSLILPYTAFHLSLTIFILEGFMRGFPKDLEESGVMDGAGVF 180 Query: 181 RIFWSIILPITRPAMATVVILNFIYNWNEYLFALVLITSNSLKTLPLGLANFVGVETASY 240 +IFWSIILPITRPA++TVVILNFIYNWNEYLFALVLI+S+SLKTLPLGLANFVG+ETAS Sbjct: 181 KIFWSIILPITRPAISTVVILNFIYNWNEYLFALVLISSSSLKTLPLGLANFVGIETASL 240 Query: 241 TLQMSALTIALVPILIFYLLLQKQLVTG 268 TLQM+ALTIAL+PI+IFYLLLQ +T Sbjct: 241 TLQMAALTIALIPIIIFYLLLQNSSLTA 268 >Q8ES67.1 Q8ES67_OCEIH Sugar ABC transporter permease Length = 290 Score = 328 bits (840), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 156/275 (56%), Positives = 198/275 (72%) Query: 2 KKKSPLFKIFAYTFLSLFAIMNIIPIFWMIVNSFKEEQEYAANPFSFPATLHFSNYTKAW 61 K K L +I YT L LFAI+N+ PI WM +NSFK EQE+ N F FP NY AW Sbjct: 16 KLKKRLSRIIIYTLLGLFAIVNVYPIIWMAINSFKSEQEFTVNQFGFPNEFILENYANAW 75 Query: 62 EVANMNIYFLNXXXXXXXXXXXXXXXXXXAAYFLARFTFRLRGFTYALFLLGMLVPIHAT 121 E+AN+ + F N A+YFLARF FR Y F+ G+L+PIHAT Sbjct: 76 EIANLGVLFKNSIFVCVTATLVTVIIGALASYFLARFKFRFNKIMYTFFIFGLLIPIHAT 135 Query: 122 LIPIFLIMQKLSLIDTYLSLILPYTAFHLSLTVFILEGFMRGFPKDLEESGIMDGAGVFR 181 L+P+F++M+ L L++T ++L+ PY AFHL +T+FIL FM+ FPKD+EES IMDGAG+FR Sbjct: 136 LVPMFILMKNLGLLNTEITLLFPYVAFHLPITIFILTSFMKAFPKDIEESAIMDGAGIFR 195 Query: 182 IFWSIILPITRPAMATVVILNFIYNWNEYLFALVLITSNSLKTLPLGLANFVGVETASYT 241 IFWSIILP++RPA+ATVVILNFIYNWNE+ FALVLI +L+TLPLGLANF G T +Y Sbjct: 196 IFWSIILPMSRPAIATVVILNFIYNWNEFSFALVLINDPALQTLPLGLANFAGQFTTNYG 255 Query: 242 LQMSALTIALVPILIFYLLLQKQLVTGMTAGAVKG 276 QM+ LT++LVPI+IFYL L+K++V GMT+GAVKG Sbjct: 256 AQMAGLTMSLVPIVIFYLFLEKEIVNGMTSGAVKG 290 >Q2BFH6.1 Q2BFH6_9BACI Sugar ABC transporter permease Length = 291 Score = 312 bits (799), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 152/276 (55%), Positives = 196/276 (71%), Gaps = 4/276 (1%) Query: 1 MKKKSPLFKIFAYTFLSLFAIMNIIPIFWMIVNSFKEEQEYAANPFSFPATLHFSNYTKA 60 +K K P+ Y L +FAI+N PI WM +NSFK E+E+A N F P+T F NY A Sbjct: 20 LKWKRPII----YAVLIIFAIVNAYPIIWMAINSFKSEKEFATNQFGLPSTFVFENYLSA 75 Query: 61 WEVANMNIYFLNXXXXXXXXXXXXXXXXXXAAYFLARFTFRLRGFTYALFLLGMLVPIHA 120 WE AN+ F N A+YFL+RF F+L Y F+ GML+PIHA Sbjct: 76 WETANLGTLFTNSLFVTITATLLTVLIGAIASYFLSRFHFKLNKAAYVFFIFGMLIPIHA 135 Query: 121 TLIPIFLIMQKLSLIDTYLSLILPYTAFHLSLTVFILEGFMRGFPKDLEESGIMDGAGVF 180 TL+P+F++M+ L L++ ++LI PY AFHL +T+FIL FM+ FPKD+EES IMDG G+F Sbjct: 136 TLVPMFILMRNLGLLNMPITLIFPYIAFHLPITIFILTSFMKAFPKDIEESAIMDGCGIF 195 Query: 181 RIFWSIILPITRPAMATVVILNFIYNWNEYLFALVLITSNSLKTLPLGLANFVGVETASY 240 RIFWSIILP++RPA+ATVVILNFIYNWNE+ FALVLI +L+TLPLGLA+F G T +Y Sbjct: 196 RIFWSIILPMSRPALATVVILNFIYNWNEFSFALVLINDPALQTLPLGLASFAGQFTTNY 255 Query: 241 TLQMSALTIALVPILIFYLLLQKQLVTGMTAGAVKG 276 QM+ LT++++PIL FYLLL+K++V GMTAGAVKG Sbjct: 256 GAQMAGLTMSMIPILAFYLLLEKEIVNGMTAGAVKG 291 >B8CXP3.1 B8CXP3_HALOH Binding-protein-dependent transport systems inner membrane component Length = 281 Score = 296 bits (757), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 136/274 (49%), Positives = 197/274 (71%) Query: 3 KKSPLFKIFAYTFLSLFAIMNIIPIFWMIVNSFKEEQEYAANPFSFPATLHFSNYTKAWE 62 K + +I YT L +FA++N+ PI WM +NS K EQE N FSFP L+ +NY +AW+ Sbjct: 8 NKKKIKQIVIYTILIIFAVVNLYPILWMAINSCKTEQEITMNSFSFPNRLYLNNYFEAWK 67 Query: 63 VANMNIYFLNXXXXXXXXXXXXXXXXXXAAYFLARFTFRLRGFTYALFLLGMLVPIHATL 122 A++ F+N +YF+ARF F+ + Y +F+ GMLVPIH TL Sbjct: 68 TAHLGNRFINSIIVSIVAMVVTVLLGALVSYFIARFQFKWKNILYVIFIFGMLVPIHGTL 127 Query: 123 IPIFLIMQKLSLIDTYLSLILPYTAFHLSLTVFILEGFMRGFPKDLEESGIMDGAGVFRI 182 +P+F++M+K+ LI+T +LI PY F+L LT+FIL +M+G P ++EE+ ++DG G+F+I Sbjct: 128 VPMFILMRKIGLINTRWALIFPYIGFNLPLTIFILVSYMKGLPTEVEEAAVVDGCGIFKI 187 Query: 183 FWSIILPITRPAMATVVILNFIYNWNEYLFALVLITSNSLKTLPLGLANFVGVETASYTL 242 FWS ILP++RPA+ATV ILNFI+NWNE+ FALVLI S+SLKT+PLGLA+F G + +Y + Sbjct: 188 FWSFILPMSRPALATVSILNFIFNWNEFSFALVLINSDSLKTIPLGLASFAGKYSTNYGV 247 Query: 243 QMSALTIALVPILIFYLLLQKQLVTGMTAGAVKG 276 QM+ALT+ L+PI+IFYL++++ L+ GM++GAVKG Sbjct: 248 QMAALTMVLIPIIIFYLIMEEHLIKGMSSGAVKG 281 >Q1AYD4.1 Q1AYD4_RUBXD Binding-protein-dependent transport systems inner membrane component Length = 285 Score = 226 bits (577), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 116/275 (42%), Positives = 157/275 (57%) Query: 2 KKKSPLFKIFAYTFLSLFAIMNIIPIFWMIVNSFKEEQEYAANPFSFPATLHFSNYTKAW 61 + + K Y FL +A + + P F M+V+S K E +PF P + Y AW Sbjct: 11 RLRDGTLKALLYAFLVAYAFVCLYPFFLMVVSSLKSTGEIFQSPFGLPESPSLHAYRMAW 70 Query: 62 EVANMNIYFLNXXXXXXXXXXXXXXXXXXAAYFLARFTFRLRGFTYALFLLGMLVPIHAT 121 E N YFLN AAY + R FR RGF Y FL G+++PI Sbjct: 71 ETGNFREYFLNSVLVTASSVALILLAGSMAAYPIGRHDFRGRGFLYLYFLGGLMLPIKLG 130 Query: 122 LIPIFLIMQKLSLIDTYLSLILPYTAFHLSLTVFILEGFMRGFPKDLEESGIMDGAGVFR 181 ++P+F +M L L DT LSL+L Y A + VF+L F R P++LEE+ +DGAG FR Sbjct: 131 VVPLFFLMTSLGLYDTRLSLVLVYAASGMPFAVFVLSAFFRTLPRELEEAARVDGAGEFR 190 Query: 182 IFWSIILPITRPAMATVVILNFIYNWNEYLFALVLITSNSLKTLPLGLANFVGVETASYT 241 I+W I+LP+ RPA+ATV I NFI WN++ F LVLI ++ L+TLP GLANF G + Sbjct: 191 IYWQIMLPLVRPALATVAIFNFIQLWNDFFFPLVLIQTDGLRTLPAGLANFFGTYQNDWA 250 Query: 242 LQMSALTIALVPILIFYLLLQKQLVTGMTAGAVKG 276 L + LTI +P+++ YL KQ+V G+TAGA KG Sbjct: 251 LIFAGLTITSLPLIVLYLFASKQIVRGLTAGATKG 285 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.144 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,448,837,954 Number of extensions: 55486123 Number of successful extensions: 206742 Number of sequences better than 1.0e-05: 6192 Number of HSP's gapped: 197132 Number of HSP's successfully gapped: 6193 Length of query: 276 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 142 Effective length of database: 1,798,280,193 Effective search space: 255355787406 Effective search space used: 255355787406 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)