BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0034.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5G3.1 B3K5G3_9BACI Binding-protein-dependent transport system... 365 3e-99 Q8ES68.1 Q8ES68_OCEIH Sugar ABC transporter permease 282 2e-74 Q2BFH7.1 Q2BFH7_9BACI Sugar ABC transporter permease 278 4e-73 B8CXP4.1 B8CXP4_HALOH Binding-protein-dependent transport system... 259 3e-67 B3K408.1 B3K408_9BACI Binding-protein-dependent transport system... 236 1e-60 >B3K5G3.1 B3K5G3_9BACI Binding-protein-dependent transport systems inner membrane component Length = 290 Score = 365 bits (936), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 192/289 (66%), Positives = 226/289 (78%) Query: 2 HTLKQTRLAIFIGLFPAXXXXXXXXXXXXXXXXXXSVMDWNGIGKMTFIGLDNYSRILTD 61 HTLK T+ AIF+GL PA S+M+W+GI M FIGLDN+ I D Sbjct: 2 HTLKHTKWAIFLGLAPALIIYICIAIFPIGLSVYYSLMNWDGITPMRFIGLDNFVDIFKD 61 Query: 62 DTFWLSVQNNVVIMLTGLVGQIPLGLIMALLLNRGLKGSGFFRTVGFMPVVISSVMVSLI 121 D FWLSV+NN++IM TG+ GQIPLGL++ALLLNRGLKGS FFRT+GF+PVVISSVMVSLI Sbjct: 62 DIFWLSVKNNIIIMFTGIAGQIPLGLLLALLLNRGLKGSSFFRTIGFLPVVISSVMVSLI 121 Query: 122 WGMIYNTEYGMLNSMLAFFGLGSWQQNWLGDMKWSMLSISVAYIWQNCGLYMVIFLAALQ 181 WGM+YNTEYGMLN++L GL SWQQNWLGD WSM+SIS+AYIWQNCGLYM+I LAALQ Sbjct: 122 WGMVYNTEYGMLNNLLGVLGLESWQQNWLGDPFWSMISISIAYIWQNCGLYMIILLAALQ 181 Query: 182 NIPDEVNEAAELDGATGFRRTLHITIPMLRSTIMVAVVYSISNSFRVFDLIQVLTGGGPA 241 NIPDEVNEAA LDGATG R + ITIPM+R T++V+VVYSISNSFRVFDLIQ++TGGGPA Sbjct: 182 NIPDEVNEAAALDGATGLSRIMRITIPMIRGTLLVSVVYSISNSFRVFDLIQIMTGGGPA 241 Query: 242 HQTEVMTLYMYNSAFMNLRYGYGSAXXXXXXXXXXXXXXXXNRIGREKD 290 H TEVMT+YMYN++FMN+R+GYGSA NR+ REK+ Sbjct: 242 HATEVMTVYMYNNSFMNMRFGYGSAVSVLILLFSLIVITIVNRLAREKE 290 >Q8ES68.1 Q8ES68_OCEIH Sugar ABC transporter permease Length = 291 Score = 282 bits (722), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 134/262 (51%), Positives = 182/262 (69%) Query: 5 KQTRLAIFIGLFPAXXXXXXXXXXXXXXXXXXSVMDWNGIGKMTFIGLDNYSRILTDDTF 64 ++++ AI IGL PA S+M+W+G MTFIGLDN+ + D F Sbjct: 5 RKSKAAIIIGLLPAFLIYAVFAIYPILQSFYYSLMEWDGFTDMTFIGLDNFRNLFEDPLF 64 Query: 65 WLSVQNNVVIMLTGLVGQIPLGLIMALLLNRGLKGSGFFRTVGFMPVVISSVMVSLIWGM 124 W SV+NN+ +++ ++GQ+P+ L ALLLNR +KG FFRTVGF+PVV+S+V++SL W + Sbjct: 65 WNSVKNNLYVVIASVLGQVPIALFFALLLNRKIKGVKFFRTVGFLPVVLSTVVISLTWSL 124 Query: 125 IYNTEYGMLNSMLAFFGLGSWQQNWLGDMKWSMLSISVAYIWQNCGLYMVIFLAALQNIP 184 IYN++ GMLN L GLG QNWLGD KW M+++ V +WQ GLY +IFLAALQN+P Sbjct: 125 IYNSQNGMLNEFLRNIGLGFMAQNWLGDTKWVMIAVLVTVVWQFVGLYFIIFLAALQNVP 184 Query: 185 DEVNEAAELDGATGFRRTLHITIPMLRSTIMVAVVYSISNSFRVFDLIQVLTGGGPAHQT 244 EV EAA++DGA+ + T ITIPM+ +I+VAV+ IS S + FDLI V+T GGPAH + Sbjct: 185 TEVLEAAKMDGASEWMTTFKITIPMIWDSIIVAVILCISGSLKTFDLIYVMTNGGPAHSS 244 Query: 245 EVMTLYMYNSAFMNLRYGYGSA 266 +VM LYM++ F NL+YGYGSA Sbjct: 245 DVMALYMFHETFNNLQYGYGSA 266 >Q2BFH7.1 Q2BFH7_9BACI Sugar ABC transporter permease Length = 291 Score = 278 bits (712), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 133/262 (50%), Positives = 182/262 (69%) Query: 5 KQTRLAIFIGLFPAXXXXXXXXXXXXXXXXXXSVMDWNGIGKMTFIGLDNYSRILTDDTF 64 K T++AI GL PA S+M+WNG M F+GLDN+ ++ D F Sbjct: 5 KGTKIAIAAGLAPALLIYLVFAILPILQSFYYSLMEWNGFSAMKFVGLDNFVQLFQDSLF 64 Query: 65 WLSVQNNVVIMLTGLVGQIPLGLIMALLLNRGLKGSGFFRTVGFMPVVISSVMVSLIWGM 124 W SV+NN+ ++L + GQ+P+ L +ALLLNR +KG FRT+GF+PVV+S+V++SL W + Sbjct: 65 WNSVKNNIYVVLASVFGQVPIALFIALLLNRKIKGLKIFRTIGFLPVVLSTVVISLTWSL 124 Query: 125 IYNTEYGMLNSMLAFFGLGSWQQNWLGDMKWSMLSISVAYIWQNCGLYMVIFLAALQNIP 184 I+N+E G++N +L GL QNWLGD K+SM+++ V IWQ GLY++IFLAALQN+P Sbjct: 125 IFNSEQGLINELLRAVGLDFLAQNWLGDTKFSMIAVLVVVIWQFVGLYLIIFLAALQNVP 184 Query: 185 DEVNEAAELDGATGFRRTLHITIPMLRSTIMVAVVYSISNSFRVFDLIQVLTGGGPAHQT 244 EV EAA++DGA+ + T ITIPM+ TI+VAV+ +IS S + FDLI V+T GGPAH T Sbjct: 185 QEVLEAAKIDGASEWTTTWKITIPMIWDTIIVAVILAISGSLKTFDLIYVMTHGGPAHST 244 Query: 245 EVMTLYMYNSAFMNLRYGYGSA 266 +VM LYM+N F L+YGYGSA Sbjct: 245 DVMALYMFNETFSKLQYGYGSA 266 >B8CXP4.1 B8CXP4_HALOH Binding-protein-dependent transport systems inner membrane component Length = 293 Score = 259 bits (661), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 130/266 (48%), Positives = 182/266 (68%), Gaps = 1/266 (0%) Query: 2 HTLKQTRLAIFIGLFPAXXXXXXXXXXXXXXXXXXSVMDWNGIGK-MTFIGLDNYSRILT 60 H K IF+G+ PA S ++WNG + F+ LDN+ + Sbjct: 3 HIFKHKNKYIFLGITPALIVYFLFVLVPIIISFYYSFIEWNGFSPDLKFVALDNFIDMFQ 62 Query: 61 DDTFWLSVQNNVVIMLTGLVGQIPLGLIMALLLNRGLKGSGFFRTVGFMPVVISSVMVSL 120 + FW+S++NN+V++L + GQ+PLGL++ALL+N+ +KGS FFRTV F+PVVIS+V++SL Sbjct: 63 NSIFWISLKNNIVVILASVFGQVPLGLLLALLVNQKIKGSHFFRTVIFLPVVISAVIISL 122 Query: 121 IWGMIYNTEYGMLNSMLAFFGLGSWQQNWLGDMKWSMLSISVAYIWQNCGLYMVIFLAAL 180 IW M+YN ++G++N L GL SW QNWLGD +M S+++ IWQ GLY VIFLAAL Sbjct: 123 IWSMVYNYQFGLINKFLRIVGLNSWAQNWLGDPNLAMYSVAITVIWQFIGLYFVIFLAAL 182 Query: 181 QNIPDEVNEAAELDGATGFRRTLHITIPMLRSTIMVAVVYSISNSFRVFDLIQVLTGGGP 240 QN+P E+ EAAE+DGA G +RT HI IP++ TI+ ++V IS S ++F LI V+T GGP Sbjct: 183 QNVPKEILEAAEIDGAHGLKRTFHIIIPIIWDTILASIVLCISGSLKIFGLIYVMTNGGP 242 Query: 241 AHQTEVMTLYMYNSAFMNLRYGYGSA 266 AH TEV+ L++Y +F +LRYGYGSA Sbjct: 243 AHSTEVLALHLYEKSFSSLRYGYGSA 268 >B3K408.1 B3K408_9BACI Binding-protein-dependent transport systems inner membrane component Length = 291 Score = 236 bits (603), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 109/227 (48%), Positives = 162/227 (71%) Query: 40 DWNGIGKMTFIGLDNYSRILTDDTFWLSVQNNVVIMLTGLVGQIPLGLIMALLLNRGLKG 99 W G G TFIGLDNY + D FW +++NN++++++ ++GQ+P+ LI+A++L + Sbjct: 40 SWKGFGDATFIGLDNYREMFKDPIFWRALRNNLILVVSSIIGQVPIALILAIVLFKNSFF 99 Query: 100 SGFFRTVGFMPVVISSVMVSLIWGMIYNTEYGMLNSMLAFFGLGSWQQNWLGDMKWSMLS 159 S F R+ FMP+V+S+V+V LIWG IY+ ++G+ N +L GL SW + WL D K +ML+ Sbjct: 100 SRFIRSAVFMPMVLSTVVVGLIWGYIYHPQFGIANYVLEMLGLESWTRAWLSDPKVNMLA 159 Query: 160 ISVAYIWQNCGLYMVIFLAALQNIPDEVNEAAELDGATGFRRTLHITIPMLRSTIMVAVV 219 IS+ W N G Y+VIF+AALQNI E+++AA++DG+TG+ + + IT+PM+ ST++V +V Sbjct: 160 ISIPINWANIGPYLVIFIAALQNISPEIHDAAKIDGSTGWNKLMQITLPMVWSTVVVTIV 219 Query: 220 YSISNSFRVFDLIQVLTGGGPAHQTEVMTLYMYNSAFMNLRYGYGSA 266 IS S + FD + V+TGGGPA TE++ YMYNS F RYGYGSA Sbjct: 220 LCISGSLKAFDHVIVMTGGGPAQSTELLATYMYNSTFSVYRYGYGSA 266 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.140 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,504,638,629 Number of extensions: 56236809 Number of successful extensions: 199847 Number of sequences better than 1.0e-05: 5842 Number of HSP's gapped: 192333 Number of HSP's successfully gapped: 5859 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)