BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0031.p (338 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5G5.1 B3K5G5_9BACI Glycosidase PH1107-related 551 e-155 B3KH99.1 B3KH99_9BACI Glycosidase PH1107-related 550 e-155 B1D5Z0.1 B1D5Z0_9BACL Glycosidase PH1107-related 382 e-104 A4XMG7.1 A4XMG7_CALS8 Glycosidase, PH1107-related 273 2e-71 C3QA42.1 C3QA42_9BACE Putative uncharacterized protein 234 1e-59 >B3K5G5.1 B3K5G5_9BACI Glycosidase PH1107-related Length = 338 Score = 551 bits (1419), Expect = e-155, Method: Compositional matrix adjust. Identities = 259/335 (77%), Positives = 296/335 (88%), Gaps = 1/335 (0%) Query: 1 MKINRHPENPIVVPGKYDWRLATVFNPAVIKDGGKFYMIERTAGSLTPCKNFLGLLESED 60 MKI RH ENPIVVPG YDWR TVFNPAVI D GKFYMIERTAGSLTPCKN+LGLLES D Sbjct: 1 MKITRHAENPIVVPGLYDWRKVTVFNPAVIIDNGKFYMIERTAGSLTPCKNYLGLLESTD 60 Query: 61 GVHFTHVKEEPVVTPDMLGFPYGSVQDPRIVKIGDIFYMTFALRPCAMNYYPTGTGIPER 120 GVHFTHV +EPVVTPD LGFPYGS+QDPRIVKI D FY+ +ALRPCAMNYYPTG GIPER Sbjct: 61 GVHFTHVLDEPVVTPDQLGFPYGSIQDPRIVKIDDTFYLNYALRPCAMNYYPTGAGIPER 120 Query: 121 SIPEYPDGWGKEEGHWLTRSILMSSRNLTDWEYVSTTTPLEINDRNNMLFPEKIGGKYAL 180 SIPEYPDGWG+EEGHWLTRS +++S++L WEY+ TTPL+INDR+N+LFPEKI GKY L Sbjct: 121 SIPEYPDGWGEEEGHWLTRSGILTSKDLIHWEYLCDTTPLDINDRDNILFPEKINGKYVL 180 Query: 181 LRRPEEYIGEAYGTEQAAIWISYSDDLKSWEAPTLLAKAENG-WENKKIGASTPPIRTEY 239 LRRPEEY+GE YGTE+AA+WISYS+DL W+ P LLAKAEN WE KKIG STPP+RT+ Sbjct: 181 LRRPEEYVGEEYGTEKAAMWISYSEDLVHWDEPKLLAKAENNDWEFKKIGGSTPPVRTDK 240 Query: 240 GWLLLYHGVDSDTVYRLGAMLLDLENPSKVIARTKEFIMEPETYYEKFGYQIPNVIFPTG 299 GWL+LYHGVD DTVYR+GA+LLDLENP K+IART+ FIMEPETYYEKFGYQIPNVIFPTG Sbjct: 241 GWLVLYHGVDQDTVYRVGALLLDLENPEKIIARTRRFIMEPETYYEKFGYQIPNVIFPTG 300 Query: 300 CVLDNGILHIYYGVTDTAIALATVPLSEMLEHLLG 334 V+ + +L+IYYGVTDTAIALATVPL ++++++L Sbjct: 301 NVVKDDLLYIYYGVTDTAIALATVPLDDLVDYILN 335 >B3KH99.1 B3KH99_9BACI Glycosidase PH1107-related Length = 338 Score = 550 bits (1418), Expect = e-155, Method: Compositional matrix adjust. Identities = 257/334 (76%), Positives = 295/334 (88%), Gaps = 1/334 (0%) Query: 1 MKINRHPENPIVVPGKYDWRLATVFNPAVIKDGGKFYMIERTAGSLTPCKNFLGLLESED 60 MKI RHP NPIVVPG Y+WR TVFNPAVI D GKFYMIERTAGSLTPCKN LGLLESED Sbjct: 1 MKITRHPNNPIVVPGTYEWRKVTVFNPAVIIDNGKFYMIERTAGSLTPCKNVLGLLESED 60 Query: 61 GVHFTHVKEEPVVTPDMLGFPYGSVQDPRIVKIGDIFYMTFALRPCAMNYYPTGTGIPER 120 GV+FTHVK+EP+VTPDMLGFPYGSVQDPRIVKI +Y+ +ALRPCAM+YYPTG GIPER Sbjct: 61 GVNFTHVKDEPIVTPDMLGFPYGSVQDPRIVKIDGTYYLNYALRPCAMSYYPTGAGIPER 120 Query: 121 SIPEYPDGWGKEEGHWLTRSILMSSRNLTDWEYVSTTTPLEINDRNNMLFPEKIGGKYAL 180 +IP+YPDGWG+EEGHWLTRS ++ S NL DWE+V+ TTPL+INDR+N+LFPEKI GKY L Sbjct: 121 TIPKYPDGWGEEEGHWLTRSSIVKSDNLIDWEFVADTTPLDINDRDNILFPEKINGKYVL 180 Query: 181 LRRPEEYIGEAYGTEQAAIWISYSDDLKSWEAPTLLAKAEN-GWENKKIGASTPPIRTEY 239 LRRPEEY+G AYGT+ AAIWI+YS+DL WE P LLAKAEN WE++KIG +TPP+RT+ Sbjct: 181 LRRPEEYVGAAYGTDSAAIWITYSEDLVHWEEPKLLAKAENPAWESRKIGGATPPVRTDK 240 Query: 240 GWLLLYHGVDSDTVYRLGAMLLDLENPSKVIARTKEFIMEPETYYEKFGYQIPNVIFPTG 299 GWL+LYHGVD VYR+GAMLLDL NP KVIARTK FIMEPETYYEKFGYQIPNV+FPTG Sbjct: 241 GWLVLYHGVDDQVVYRVGAMLLDLNNPEKVIARTKTFIMEPETYYEKFGYQIPNVVFPTG 300 Query: 300 CVLDNGILHIYYGVTDTAIALATVPLSEMLEHLL 333 V+ +G+L+IYYGVTDTAIALATVP+ E++EH+L Sbjct: 301 NVVKDGLLYIYYGVTDTAIALATVPVDELVEHIL 334 >B1D5Z0.1 B1D5Z0_9BACL Glycosidase PH1107-related Length = 338 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 190/337 (56%), Positives = 248/337 (73%), Gaps = 1/337 (0%) Query: 1 MKINRHPENPIVVPGKYDWRLATVFNPAVIKDGGKFYMIERTAGSLTPCKNFLGLLESED 60 MK+ RH NPIVVPG DWR A VFNP V++ GK+YM ER AGSL P + +GLLESED Sbjct: 1 MKLTRHENNPIVVPGGADWRKAAVFNPGVVQADGKYYMYERAAGSLRPFQTCIGLLESED 60 Query: 61 GVHFTHVKEEPVVTPDMLGFPYGSVQDPRIVKIGDIFYMTFALRPCAMNYYPTGTGIPER 120 GV+F V +EPV T +MLG+P GSV+D R+V+I ++YM +AL+P + +PTGTG+P Sbjct: 61 GVNFRPVLDEPVFTAEMLGYPGGSVEDARVVRIDGLYYMCYALQPYGFDCWPTGTGLPAY 120 Query: 121 SIPEYPDGWGKEEGHWLTRSILMSSRNLTDWEYVSTTTPLEINDRNNMLFPEKIGGKYAL 180 YP+ +TRS + S + ++ +S TTP EI+DR+N LFPEKIGG++A+ Sbjct: 121 YPEHYPEWADNGVEPMITRSGIAVSVDGIHFKPLSYTTPAEIDDRDNALFPEKIGGRFAI 180 Query: 181 LRRPEEYIGEAYGTEQAAIWISYSDDLKSWEAPTLLAKAEN-GWENKKIGASTPPIRTEY 239 LRRP +Y+GE YGT +WISYSD+L W P L+A AEN WE KIGA+ PIRT+ Sbjct: 181 LRRPLQYVGEKYGTNSPGMWISYSDNLFDWTEPELVAVAENPEWEGTKIGAAATPIRTDR 240 Query: 240 GWLLLYHGVDSDTVYRLGAMLLDLENPSKVIARTKEFIMEPETYYEKFGYQIPNVIFPTG 299 GWL+LYHGVD+ +VYR+GA+LLDLE+P KVIART+E IMEPETYYEKFG +PNV+FPT Sbjct: 241 GWLVLYHGVDARSVYRVGALLLDLEDPKKVIARTREPIMEPETYYEKFGLVLPNVVFPTA 300 Query: 300 CVLDNGILHIYYGVTDTAIALATVPLSEMLEHLLGAA 336 ++ +G+L+IYYG DT+IALATVPL+E+L+ + A Sbjct: 301 NLVIDGLLYIYYGCCDTSIALATVPLNELLDAMTDEA 337 >A4XMG7.1 A4XMG7_CALS8 Glycosidase, PH1107-related Length = 333 Score = 273 bits (697), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 157/338 (46%), Positives = 206/338 (60%), Gaps = 16/338 (4%) Query: 1 MKINRHPENPIVVPG-KYDWRLATVFNPAVIKDGGKFYMIERTAGSLTPCKNFLGLLESE 59 MK+ ++ NPI+ P +W V NP V + GKFYM+ R AG GL SE Sbjct: 1 MKLKKYKNNPILKPNPTNEWESLAVCNPGVWYENGKFYMLYRAAGHDLTHYVHFGLAISE 60 Query: 60 DGVHFTHVKEEPVVTPDMLGFPYGSVQDPRIVKIGDIFYMTFALRPCAMNYYPTGTGIPE 119 DG +F V ++PV++P G G V+DPRIVK GDIFY+T+A R YP G + Sbjct: 61 DGFNFERVSKKPVLSPSPDGPDAGCVEDPRIVKYGDIFYITYAYRA-----YPPGQYWNK 115 Query: 120 RSIPEYPDGWG-----KEEGHWLTRSILMSSRNLTDWEYVSTTTPLEINDRNNMLFPEKI 174 I Y KE +T S L+ S++L ++ + T ++DR+ +LFPEKI Sbjct: 116 NKIKTYEPNENMPVCLKEN---ITNSGLLMSKDLIHFKRLGRITKANLDDRDVILFPEKI 172 Query: 175 GGKYALLRRPEEYIGEAYGTEQAAIWISYSDDLKSWEAPTLLAKAENGWENKKIGASTPP 234 GKY +L RP +++G YG E +IWIS+S+DL WE LLAKAE WE KIG STPP Sbjct: 173 NGKYVMLHRPMQWVGPKYGCEYPSIWISFSEDLMVWEESKLLAKAEYEWET-KIGGSTPP 231 Query: 235 IRTEYGWLLLYHGVDSDTVYRLGAMLLDLENPSKVIARTKEFIMEPETYYEKFGYQIPNV 294 I+TE GWL LYH VD +YR+GAMLLDL++P+ +IARTKEFIMEPE YE G Sbjct: 232 IKTEEGWLTLYHAVDKKGIYRVGAMLLDLKDPTIIIARTKEFIMEPEYEYETKGIY-NGC 290 Query: 295 IFPTGCVLDNGILHIYYGVTDTAIALATVPLSEMLEHL 332 +FPTG V+ +GIL+IYYG D +AT+ L +LE+L Sbjct: 291 VFPTGNVVVDGILYIYYGAADMYCCVATIELEAILEYL 328 >C3QA42.1 C3QA42_9BACE Putative uncharacterized protein Length = 341 Score = 234 bits (596), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 136/339 (40%), Positives = 192/339 (56%), Gaps = 9/339 (2%) Query: 1 MKINRHPENPIVVPG-KYDWRLATVFNPAVIKDGGKFYMIERTAGSLTPCKNFLGLLESE 59 MK+ + NPI+ P K DW NP V D G FYM+ R AG+ LGL S Sbjct: 1 MKLKKFEGNPILSPSEKNDWESLVTCNPGVFYDNGMFYMLYRAAGNDREHVIRLGLATSR 60 Query: 60 DGVHFTHVKEEPVVTPDMLGFPYGSVQDPRIVKIGDIFYMTFALRPCAMNYYPTGTGIPE 119 DG +F + P +P + G G V+DPRIVK +Y+T+A RP Y G E Sbjct: 61 DGFYFERHSDRPAFSPSVDGPDSGCVEDPRIVKFDGEYYITYAYRPVPPGQY-WKFGHDE 119 Query: 120 RSIPEYPDGWGKEEGHWLTRSILMSSRNLTDWEYVSTTTPLEINDRNNMLFPEKIGGKYA 179 +PE L + L + + + + T ++DR+ +LFPEK+ G++ Sbjct: 120 VLLPECGKYAPAAIAKNLGNTGLAVTTDFCHFRRLGRLTSPVLDDRDVILFPEKVNGQFV 179 Query: 180 LLRRPEEYIGEAYGTEQAAIWISYSDDLKSWEAPT---LLAKAENGWENKKIGASTPPIR 236 +L RP++YIGE +G E +IWI +SDDL WE LL EN WE +KIG STPP++ Sbjct: 180 MLHRPKQYIGEKFGVEYPSIWIKFSDDLLDWEGKESHLLLTGRENTWE-EKIGGSTPPLK 238 Query: 237 TEYGWLLLYHGVD--SDTVYRLGAMLLDLENPSKVIARTKEFIMEPETYYEKFGYQIPNV 294 T+ GWL+LYHGV+ YR+GA+LLDL+NP K++A+T E I+EPE +E GY Sbjct: 239 TDKGWLMLYHGVEHGGKGYYRVGALLLDLDNPLKILAKTHESILEPEEDFEINGY-YNGC 297 Query: 295 IFPTGCVLDNGILHIYYGVTDTAIALATVPLSEMLEHLL 333 +FPTG V+ + L +YYG D + +AT ++E+L +LL Sbjct: 298 VFPTGNVIVDDTLFVYYGSADKYVCVATCSVNELLSYLL 336 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,339,918,451 Number of extensions: 105317978 Number of successful extensions: 197541 Number of sequences better than 1.0e-05: 300 Number of HSP's gapped: 196634 Number of HSP's successfully gapped: 303 Length of query: 338 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 202 Effective length of database: 1,779,438,163 Effective search space: 359446508926 Effective search space used: 359446508926 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)