BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0030.p (459 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFM6.1 B3KFM6_9BACI Polysaccharide deacetylase 575 e-162 B1DIU6.1 B1DIU6_9BACL Polysaccharide deacetylase 469 e-130 A4J279.1 A4J279_DESRM Polysaccharide deacetylase 241 1e-61 A5D124.1 A5D124_PELTS Hypothetical membrane protein 224 1e-56 C1P831.1 C1P831_BACCO Polysaccharide deacetylase 214 2e-53 >B3KFM6.1 B3KFM6_9BACI Polysaccharide deacetylase Length = 460 Score = 575 bits (1481), Expect = e-162, Method: Compositional matrix adjust. Identities = 261/432 (60%), Positives = 345/432 (79%) Query: 1 MQTLLLWLFYISTFYAFIPGMISRIFGYRVFRKGIGRSEFALTFDDGPDSLYTPXXXXXX 60 MQTLLLWLFY+S+FYAFIPG+++RIFG+RVFR+G G +FALTFDDGPD YTP Sbjct: 1 MQTLLLWLFYLSSFYAFIPGILTRIFGFRVFRRGTGLEDFALTFDDGPDPFYTPRLLDLL 60 Query: 61 KRYGAKATFFVVGSHAEQNPEIIKRMHDEGHLIGIHNYVHKTNWLMRPATVKRQIQHTDD 120 KRY KATFF+VGSHAE++PE++KR+H EGHLIGIHNYVHK+NWLMRP TV++Q++ TDD Sbjct: 61 KRYNMKATFFLVGSHAEKHPEVVKRIHSEGHLIGIHNYVHKSNWLMRPVTVRKQVKRTDD 120 Query: 121 IIFSITGERSTYYRPPWGIVNLFDFSKRRQVQIVLWSAMFGDWKEKLGADRLTEKLLAKL 180 II++ITGERS YYRPPWGIVNLFDF+K +I+LWS+MF DW+ K+GAD+LT+++LAKL Sbjct: 121 IIYAITGERSRYYRPPWGIVNLFDFAKNTGCRIILWSSMFNDWRSKIGADKLTQRMLAKL 180 Query: 181 NPGEVMLLHDCGTTLGADPNAPEHMLVALERMLQEAEGRGLRSIRIDEMIRMVQSSPVQK 240 N GEVMLLHDCGTT+GA+P+APE ML+ALER+L+ AE +G+RSIRID+M+ + +K Sbjct: 181 NGGEVMLLHDCGTTMGANPDAPEQMLIALERVLKVAEQKGIRSIRIDDMMAKESKAKAKK 240 Query: 241 LSFGKRLLVGLWLAWEQVFQLMLRIKTISPADPFLHYRMRKYKGEPVQMEGGEWLAKGDK 300 LS KRL+V LWL WE+ F + ++T++P DP LH+R R + G+ V ME G L KGD+ Sbjct: 241 LSPLKRLIVSLWLLWEKAFHAVFALETVNPEDPMLHFRKRAFTGKTVVMEDGSRLEKGDQ 300 Query: 301 VIELHIDNRQLFELGIHSRSSAQLAIRMIRRMEKDMPVLAQRIAADIDLAEAKALYGVSM 360 V+ELH DN++LFE+G SRS QLAI+MIR ++KD+P LA + + + K LY V+M Sbjct: 301 VLELHFDNKKLFEIGSRSRSEVQLAIKMIRAVQKDLPSLANMVLDRPEFKDIKGLYAVTM 360 Query: 361 INRGPEKFGFMVLDLPKGWFARSTKFYLSILLSVIHPAGGARLKERSEVLVPKMMLMPVS 420 I+RGPE+FGF V+DLP+G FA S++ YL +LLSVIHP G ARLKE ++++ PKM++MPV Sbjct: 361 ISRGPEQFGFHVMDLPRGLFASSSRLYLKLLLSVIHPKGQARLKEGTQMMEPKMLVMPVD 420 Query: 421 QLLDQMNQQRPQ 432 L+ + +++ Q Sbjct: 421 VLIGRYAEKKTQ 432 >B1DIU6.1 B1DIU6_9BACL Polysaccharide deacetylase Length = 467 Score = 469 bits (1208), Expect = e-130, Method: Compositional matrix adjust. Identities = 229/445 (51%), Positives = 305/445 (68%), Gaps = 6/445 (1%) Query: 1 MQTLLLWLFYISTFYAFIPGMISRIFGYRVFRKGIGRSEFALTFDDGPDSLYTPXXXXXX 60 M LLLW FY + YAFIP +ISR+FG+RVF+KG SE ALTFDDGPD YTP Sbjct: 1 MLQLLLWAFYFLSLYAFIPALISRVFGFRVFKKGHVASEIALTFDDGPDPEYTPQLLDLL 60 Query: 61 KRYGAKATFFVVGSHAEQNPEIIKRMHDEGHLIGIHNYVHKTNWLMRPATVKRQIQHTDD 120 RY AKATFFVVGSHAE++PEI++RMH+EGH+I IHNYVHKTNW MRP TV+ QI T D Sbjct: 61 ARYKAKATFFVVGSHAEKHPEILRRMHEEGHIIAIHNYVHKTNWFMRPKTVRSQIDRTSD 120 Query: 121 IIFSITGERSTYYRPPWGIVNLFDFSKRRQVQIVLWSAMFGDWKEKLGADRLTEKLLAKL 180 +I G RS +YRPPWGIVN+FDF R ++QI+LWSA+FGDW KLGA+RL +K++ K+ Sbjct: 121 VIREAVGIRSAFYRPPWGIVNVFDFMNRNKMQIILWSAIFGDWNVKLGAERLKQKMMKKM 180 Query: 181 NPGEVMLLHDCGTTLGADPNAPEHMLVALERMLQEAEGRGLRSIRIDEMIRMVQSSPVQK 240 PGEV+LLHDCG T GADP AP +ML+AL+ + EA RG R + I EM+++ +P K Sbjct: 181 RPGEVLLLHDCGRTPGADPTAPANMLIALKAYMDEATKRGFRFVGIAEMMKLSDKTP--K 238 Query: 241 LSFGKRLLVGLWLAWEQVFQLMLRIKTISPADPFLHYRMRKYKGEPVQMEGGEWLAKGDK 300 S K +VG WL +E+++ ++ R+KT+ D HYR+ KY GEPV++ +L GDK Sbjct: 239 RSVIKNAMVGAWLLYEKLYHVVFRLKTVGGEDAVFHYRVIKYHGEPVKLADNGYLKNGDK 298 Query: 301 VIELHIDNRQLFELGIHSRSSAQLAIRMIRRMEKDMPVLAQRIAADIDLAEAKALYGVSM 360 ++ELH DN+ L + + S S IR++R ME+ +P LA+ +A D D+ +A+YGV+M Sbjct: 299 IVELHFDNKMLSSISLQSSSPLSTGIRLLREMEQALPALARTLAKDPDVEGVRAIYGVTM 358 Query: 361 INRGPEKFGFMVLDLPKGWFARSTKFYLSILLSVI-HPAGGARLKERSEVLVPKMMLMPV 419 I+RG ++ GF V LP G FAR+T+ YL IL SV+ + AR K + + + P M+LMP+ Sbjct: 359 IHRGADRLGFQVEPLPDGMFARATRIYLKILTSVLTNKQRNARGKAKRDSIKPHMLLMPL 418 Query: 420 SQLL---DQMNQQRPQKQVKPREQV 441 QLL K VKP ++V Sbjct: 419 EQLLMFAVNGAHASTTKGVKPHQEV 443 >A4J279.1 A4J279_DESRM Polysaccharide deacetylase Length = 425 Score = 241 bits (614), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 142/416 (34%), Positives = 229/416 (55%), Gaps = 15/416 (3%) Query: 15 YAFIPGMISRIFGYRVFRKGIGRS-EFALTFDDGPDSLYTPXXXXXXKRYGAKATFFVVG 73 Y +P + R+ +R+G S + ALTFDDGPD L+T ++Y AKA+FFVVG Sbjct: 16 YILLPTFLGRVLSIGSYRRGNKHSGKVALTFDDGPDPLFTAQVLDILQQYNAKASFFVVG 75 Query: 74 SHAEQNPEIIKRMHDEGHLIGIHNYVHKTNWLMRPATVKRQIQHTDDIIFSITGERSTYY 133 +HA+++PE+I+R+ EGH +G H Y H WL P R+I I I G+ Y+ Sbjct: 76 NHAKKHPELIQRILAEGHSLGTHGYHHSFAWLQGPIASIREIMLGHKSIALIDGKLPLYF 135 Query: 134 RPPWGIVNL--FDFSKRRQVQIVLWSAMFGDWKEKLGADRLTEKLLAKLNPGEVMLLHDC 191 RP WG+ NL + ++ Q + VLW+ M DW K A+ + + + K G +++ HD Sbjct: 136 RPSWGVFNLATYLYTLLSQQKTVLWTFMTWDWDAKSSAESIHDIVTRKTRSGSIIVFHDR 195 Query: 192 GTTLGADPNAPEHMLVALERMLQEAEGRGLRSIRIDEMIRMVQSSPVQKLSFGKRLLVGL 251 T GA P M+ AL +L+E + +GL + +++++ + F K+ L L Sbjct: 196 CTGFGAAAEGPVKMVQALPIILKELKCKGLEPVNLEQLMLY------DDVGFIKKNLRRL 249 Query: 252 WLAWEQVFQLMLRIKTISPADPFLHYRMRKYKGEPVQMEGGEWLAKGDKVIELHIDNRQL 311 W WE F + +K + F +R Y+G+ +++ G L+ GDKVIELH++N L Sbjct: 250 WQVWELCFDRIAGLKPVGNEGLF-RLAVRSYRGQTLKLPDGTLLSPGDKVIELHLNNDFL 308 Query: 312 FELGIHSRSSAQLAIRMIRRMEKDMPVLAQRIAADIDLAEAKALYGVSMINRGPEKFGFM 371 +L +RS AI ++R + +P+LA+ I+ D KAL G++MI+RG + GF Sbjct: 309 QQLTTKARSLEATAIALLRETRRSLPLLAKFISQDPACNGTKALIGITMIHRGTPQLGFT 368 Query: 372 VLDL-PKGWFARS-TKFYLSILLSVIHPAGGARLKERSEVLVPKMMLMPVSQLLDQ 425 V DL P+ RS +Y LL ++HP G + ++ + LVPK +++ ++LL++ Sbjct: 369 VYDLSPR---IRSLVAWYQRWLLFLMHPGGLSHIRRQWSKLVPKKVIITRNELLNR 421 >A5D124.1 A5D124_PELTS Hypothetical membrane protein Length = 446 Score = 224 bits (571), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 130/419 (31%), Positives = 218/419 (52%), Gaps = 9/419 (2%) Query: 9 FYISTFYAFIPGMISRIFGY-RVFRKGIGRSEFALTFDDGPDSLYTPXXXXXXKRYGAKA 67 F YA +P + R+ G + R G+ ALTFDDGPD YTP +Y KA Sbjct: 10 FLFLLIYAVVPTALVRLSGIGAISRIPRGKKRIALTFDDGPDPRYTPQILNILGQYRVKA 69 Query: 68 TFFVVGSHAEQNPEIIKRMHDEGHLIGIHNYVHKTNWLMRPATVKRQIQHTDDIIFSITG 127 FFV+GS A +PEII+++ GH IG H + HK WL+ P R+I T+ I +TG Sbjct: 70 CFFVIGSKARAHPEIIRQIVQAGHEIGNHGFSHKAAWLLGPFATTREIAETNRAIEELTG 129 Query: 128 ERSTYYRPPWGIVNLFDFS--KRRQVQIVLWSAMFGDWKEKLGADRLTEKLLAKLNPGEV 185 +++ + RP WG+ NLF + + +++VLW+ M DW + + + K+L +L G + Sbjct: 130 QKARFCRPAWGLFNLFSLCYFRLKGLKVVLWTYMSWDWIRRATPESICRKVLGRLRDGAI 189 Query: 186 MLLHDCGTTLGADPNAPEHMLVALERMLQEAEGRGLRSIRIDEMIRMVQSSPVQKLSFGK 245 ++LHD + GA +P H++ AL +L+ +GL+ + ++E+ + S L+ G Sbjct: 190 LVLHDGDSAPGAAKGSPAHVVEALPAILEGLRQKGLKVVPLEELNGL--KSRRAALAKGV 247 Query: 246 RLLVGLWLAWEQVFQLMLRIKTISPADPFL-HYRMRKYKGEPVQMEGGEWLAKGDKVIEL 304 R+ LW E+ +L I+ + + +R+Y+G+ + G L G++ +EL Sbjct: 248 RM---LWSLLEKAIRLCSGIRDLGDGKSSIWRIALRRYRGKEWHLPDGSVLRPGEQFLEL 304 Query: 305 HIDNRQLFELGIHSRSSAQLAIRMIRRMEKDMPVLAQRIAADIDLAEAKALYGVSMINRG 364 HI+N +L L S S + + ++ + + +A + D + L G+++++RG Sbjct: 305 HINNERLLSLVDRSTSLERATLIALKEIRNGLADMACILMNDRRFCNIEVLLGITLLHRG 364 Query: 365 PEKFGFMVLDLPKGWFARSTKFYLSILLSVIHPAGGARLKERSEVLVPKMMLMPVSQLL 423 E GF V D+ G F T +Y LL++ HP G LK E L PK +++ +L+ Sbjct: 365 AELIGFTVFDMKPGLFRTVTGWYEQWLLALFHPGGFKNLKAYREKLTPKYVVISRRELV 423 >C1P831.1 C1P831_BACCO Polysaccharide deacetylase Length = 247 Score = 214 bits (544), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 101/229 (44%), Positives = 145/229 (63%) Query: 1 MQTLLLWLFYISTFYAFIPGMISRIFGYRVFRKGIGRSEFALTFDDGPDSLYTPXXXXXX 60 M+ ++ L YA IP +++ G+ V KG +E ALTFDDGPD +YTP Sbjct: 1 MEVIIWVLILFILIYAIIPYVLAAKLGFWVCWKGKKDAEIALTFDDGPDPVYTPVLLDLL 60 Query: 61 KRYGAKATFFVVGSHAEQNPEIIKRMHDEGHLIGIHNYVHKTNWLMRPATVKRQIQHTDD 120 KR KATFF+VG A + PE++ RM EGH IG+HNY H+ NWL+ P R ++ + Sbjct: 61 KRERIKATFFLVGERAARYPELVLRMSREGHCIGLHNYKHQCNWLISPWKNARTLEQSAR 120 Query: 121 IIFSITGERSTYYRPPWGIVNLFDFSKRRQVQIVLWSAMFGDWKEKLGADRLTEKLLAKL 180 II +ITGER +YRPPWG+++L DF +Q ++V WS MF DWK K G+ +++ L+ ++ Sbjct: 121 IIENITGERPVFYRPPWGMMHLLDFFLHKQFRMVHWSKMFRDWKRKGGSKKVSNGLITRV 180 Query: 181 NPGEVMLLHDCGTTLGADPNAPEHMLVALERMLQEAEGRGLRSIRIDEM 229 G+V+LLHDCG T GAD +AP++ + L + + RG R +R+DEM Sbjct: 181 ESGDVILLHDCGVTPGADEDAPQYTIEGLRVAIPALKARGFRFVRMDEM 229 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.139 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,709,199,655 Number of extensions: 110540659 Number of successful extensions: 271625 Number of sequences better than 1.0e-05: 2189 Number of HSP's gapped: 269978 Number of HSP's successfully gapped: 2189 Length of query: 459 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 320 Effective length of database: 1,751,175,118 Effective search space: 560376037760 Effective search space used: 560376037760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)