BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0029.p (601 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFM4.1 B3KFM4_9BACI Peptidoglycan glycosyltransferase EC=2.4.1... 484 e-134 B1DIU8.1 B1DIU8_9BACL Penicillin-binding protein transpeptidase 425 e-117 C0ZAS7.1 C0ZAS7_BREBN Penicillin binding protein 333 4e-89 C5D4Y3.1 C5D4Y3_9BACI Penicillin-binding protein transpeptidase 228 2e-57 C2WCE9.1 C2WCE9_BACCE Putative uncharacterized protein 213 5e-53 >B3KFM4.1 B3KFM4_9BACI Peptidoglycan glycosyltransferase EC=2.4.1.129 Length = 596 Score = 484 bits (1245), Expect = e-134, Method: Compositional matrix adjust. Identities = 259/573 (45%), Positives = 349/573 (60%), Gaps = 25/573 (4%) Query: 33 QLLLKDRKVPGAKYSMAQMAAIQSEREVVLDSGRGRLYDRSGQPLAGETVWTXXXXXXXX 92 QL++K ++VPG++++M + + IQ ER +VLDSGRG DR+G+PL G +WT Sbjct: 31 QLVMKHQRVPGSRHTMVEASLIQRERGIVLDSGRGHFTDRNGKPLTGNLIWTAVLFPA-- 88 Query: 93 XXXXXXXXXXGKEKLR-------QLATVLGVTYEQLQAERSGLKEPLLWPSGSGKNPLEL 145 G+++LR +LA +LG QL LKEP LW + P L Sbjct: 89 ----------GEKELRSAHHQVAKLARILGTDTNQLLQIWGKLKEPQLWHGADSRIPRSL 138 Query: 146 TPGQAKAVEALAVGGVRALPFARRYTGEATGRQWLGYLSXXXXXXXXXSPTGLRVPWTGT 205 + Q K + L + ++ LP+ +RY +G QWLG++S +GT Sbjct: 139 SKEQIKQIRELELPKLKVLPYEQRYGNRQSGMQWLGFVSGQRKESLFFQDY---PEISGT 195 Query: 206 AGLERTLEPLMQGVGHTEAYAQVDARGSRLPDSPIKVRAPGNPYYPLSLYTTIDKKLQEN 265 +GLE+T++ L++G G T Y V++ + D V+AP N YYPL + TTID +Q Sbjct: 196 SGLEKTMDSLIRGEGPTVVYFPVNSTNEVIQDMKPMVKAPDNRYYPLRVSTTIDIDIQRG 255 Query: 266 IEKLAVKSGVKEGAVVVLDATTGDIEAMVSLPFYNPAQISPEGGEWNNRALQAAVPGSIF 325 IE + K+G+KEGAVVVLDA DI AMVS PFYNP QI P+ G+W NR L+AA PGS+F Sbjct: 256 IETITEKAGMKEGAVVVLDARNADIVAMVSRPFYNPEQIHPKDGQWENRGLKAATPGSVF 315 Query: 326 KIVTAAAALEAGVTSPEEKFYCSGHYGKYGLSCLNGKGHGSLTMAQGFAVSCNTVFASLA 385 K+VTAAA LE G++SP E F+C G +GK+GLSC GHG++ + +GFA SCN VFA+LA Sbjct: 316 KLVTAAAILENGLSSPNEHFHCDGEFGKFGLSCWKKGGHGTIDLREGFAESCNVVFATLA 375 Query: 386 ERXXXXXXXXXXXXXXXXRDISWQAENTLGLPLLRPLSGEQPGAIFT-TLLPDDGGARVQ 444 ER + + WQ++ LGL PL E+ G +F+ + D G RVQ Sbjct: 376 ERLTPQQMTSAASALGFGQSVGWQSQQFLGLRSFAPLDHEEKGTVFSGSADASDAGVRVQ 435 Query: 445 TAIGQRDVRVTPLQAANLLVTLLHGGEVHAPRILQRVAFANGQTLKEL-PGHLAPSPQGR 503 T IGQRD V+PLQAAN +VTLLHGG PRI++++ +ANGQ L++ P + P+ G Sbjct: 436 TGIGQRDAAVSPLQAANAMVTLLHGGVKTKPRIVKQIRYANGQLLQDTNPLYEGPAGNG- 494 Query: 504 ISPPTAHVLLSLLRKVVTGGTGKSLQGSKWPLAGKSGTAQTWVKGMPRNNQWFIGYGPVG 563 IS T+ +LL + VV GTG +L KW LAGKSGTAQ VKG RNNQWF+GYGPV Sbjct: 495 ISRETSSILLEWMEDVVQEGTGSALNHRKWTLAGKSGTAQVKVKGRARNNQWFVGYGPVE 554 Query: 564 HPRFAVSVLVENVAPGSPHAATRLFGQVFDLLA 596 PR+AV+VLVEN + S + AT +FGQV D LA Sbjct: 555 KPRYAVAVLVENRSTNSSNQATEIFGQVSDFLA 587 >B1DIU8.1 B1DIU8_9BACL Penicillin-binding protein transpeptidase Length = 607 Score = 425 bits (1093), Expect = e-117, Method: Compositional matrix adjust. Identities = 244/564 (43%), Positives = 319/564 (56%), Gaps = 13/564 (2%) Query: 44 AKYSMAQMAAIQSEREVVLDSGRGRLYDRSGQPLAGETVWTXXXXXXXXXXXXXXXXXXG 103 ++ S +M+ +Q ER +VLDSGRG DR +P+ GET +T Sbjct: 42 SRTSWKRMSVMQRERNLVLDSGRGDFVDRYNRPITGETYYTLALFPVNMKFGDSEAEKKW 101 Query: 104 KEKLRQLATVLGVTYEQLQAERSGLKEPLLWPSGSGKNPLELTPGQAKAVEALAVGGVRA 163 + ++QLA +L VT QL + L+EP W SG K P L+ Q + + L + GVRA Sbjct: 102 HQNVQQLAAMLNVTERQLVSWWQELREPSFWHSGKEKLPYRLSDKQLQKLALLHLNGVRA 161 Query: 164 LPFARRYTGEATGRQWLGYLSXXXXXXXXXSPTGLRV------PWTGTAGLERTLEPLMQ 217 LP+ RY +Q LGY+S L G +GLE++L+ L+ Sbjct: 162 LPYRNRYLTAFAAKQALGYISQHPEHLELAYAKELESGKRKINELIGGSGLEKSLDDLLH 221 Query: 218 GVGHTEAYAQVDARGSRLPDSPIKVRAPGNPYYPLSLYTTIDKKLQENIEKLAVKSGVKE 277 G G T D L +V PGNPYYPL + TT+D +LQ +E ++E Sbjct: 222 GAGATSVSYFTDGNKGPLLGLNYRVTGPGNPYYPLQVQTTLDLELQNRLEAYIDAKPLRE 281 Query: 278 GAVVVLDATTGDIEAMVSLPFYNPAQISPEGGEWNNRALQAAVPGSIFKIVTAAAALEAG 337 GAVVVLDA GDI AM+S P P Q+ E EW N A+QAAVPGSIFK+VT AAALEAG Sbjct: 282 GAVVVLDAKNGDIVAMISRPQMEPLQLDNEK-EWANHAIQAAVPGSIFKLVTEAAALEAG 340 Query: 338 VTSPEEKFYCSGHYGKYGLSCLNGKGHGSLTMAQGFAVSCNTVFASLAERXXXXXXXXXX 397 VT+ +E+F C+G YGKYGLSC GHG+L++ +G A SCN VFA++AER Sbjct: 341 VTNEKERFTCNGEYGKYGLSCWKEGGHGTLSLQEGLAQSCNIVFATIAERLSAQQLQRTA 400 Query: 398 XXXXXXRDISWQAENTLGLPL---LRPLSGEQPGAIFTTL--LPDDGGARVQTAIGQRDV 452 + W +E G P LR L E+ G +F + + DGG Q+AIGQRDV Sbjct: 401 AALGIGKQAGWHSERAFG-PFRHPLRLLGEEEAGRLFPAVAGVKTDGGVMAQSAIGQRDV 459 Query: 453 RVTPLQAANLLVTLLHGGEVHAPRILQRVAFANGQTLKELPGHLAPSPQGRISPPTAHVL 512 +TPLQAANL+VTLL G V PR+++ + +ANGQ + +P AP+ GRI P TA L Sbjct: 460 LMTPLQAANLVVTLLGHGRVMEPRLVREIRYANGQRMVTMPAKSAPAADGRIRPATARKL 519 Query: 513 LSLLRKVVTGGTGKSLQGSKWPLAGKSGTAQTWVKGMPRNNQWFIGYGPVGHPRFAVSVL 572 L + VV GTG+S++ W +AGKSGTA+ G PR NQWF GYGP PR+AV+VL Sbjct: 520 LRGMEAVVDHGTGRSIRQGLWAVAGKSGTAEITRAGAPRVNQWFTGYGPAQKPRYAVAVL 579 Query: 573 VENVAPGSPHAATRLFGQVFDLLA 596 EN P S + AT LF V D+LA Sbjct: 580 AENRPPDSRNQATELFRGVMDILA 603 >C0ZAS7.1 C0ZAS7_BREBN Penicillin binding protein Length = 608 Score = 333 bits (853), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 201/564 (35%), Positives = 289/564 (51%), Gaps = 25/564 (4%) Query: 47 SMAQMAAIQSEREVVLDSGRGRLYDRSGQPLAGETVWTXXXXXXXXXXXXXXXXXXGKEK 106 + + A Q ++ +VL SGRG + DR+G GE G + Sbjct: 46 DLVKAAVKQRQQSIVLHSGRGDIVDRNGYAFTGEE------HLALILFPLARGSLQGTDG 99 Query: 107 LRQLATVLGVTYEQLQAERSGLKEPLLWPSGSGKNPLELTPGQAKAVEALAVGGVRALPF 166 L++LA + G + E+L K PLL SGK + LT QA+ + ALA+ GV AL Sbjct: 100 LQRLAQITGESKERLSETMEKAKVPLLMREASGKLVM-LTERQAEEINALAIPGVVALAV 158 Query: 167 ARRYTGEATGRQWLGYLSXXXXXXXXXSPTGLRVPWT----------GTAGLERTLEPLM 216 RY + + +G++ P WT G +GLER+ + + Sbjct: 159 TERYRADEVAKHLIGFIHKNPNMVQKLYPD----EWTSGKMNAESTLGASGLERSFDRFL 214 Query: 217 QGVGHTEAYAQVDARGSRLPDSPIKVRAPGNPYYPLSLYTTIDKKLQENIEKLAVKSGVK 276 QGV + VD G L I+ N YYPLSL TT++ +Q +E A + G+K Sbjct: 215 QGVEPSVLSYYVDGHGQPLRGLDIRYTKQSNQYYPLSLTTTLNAAIQRRMEAAADQVGLK 274 Query: 277 EGAVVVLDATTGDIEAMVSLPFYNPAQISPEGGEWNNRALQAAVPGSIFKIVTAAAALEA 336 EG++VVLD T D+ A VS P Y+ ++ +W NRA + PGS++K V AAAAL Sbjct: 275 EGSIVVLDVQTADVLAAVSRPTYDQTNVTGNTSDWKNRAFKQLPPGSVYKTVVAAAALAE 334 Query: 337 GVTSPEEKFYCSGHYGKYGLSCLNGKGHGSLTMAQGFAVSCNTVFASLAERXXXXXXXXX 396 G+ SP ++F C+G YGKY SC +GHGS+T+ + +A SCN A +A+ Sbjct: 335 GIVSPTDRFSCTGEYGKYQFSCWKKEGHGSVTLEEAYAHSCNIAIAHIAKMVGGEKLEEY 394 Query: 397 XXXXXXXRDISWQAENTLGLPLLRPLSGEQPGAIFTT-LLPDDGGARVQTAIGQRDVRVT 455 + + + + L GE+PG +F + DD G +Q+AIGQRDVR+T Sbjct: 395 AKKLGLTTQVGHLTPHLYKIQNFKQLDGEEPGRVFAEGISRDDEGVLIQSAIGQRDVRIT 454 Query: 456 PLQAANLLVTLLHGGEVHAPRILQRVAFANGQTLKELPGHLAPSPQGRISPPTAHVLLSL 515 PLQAAN++VT+L GG+ R+++ +A+ NGQ+ P S G I TA+ L + Sbjct: 455 PLQAANMMVTILRGGQSSQVRLVKEIAYRNGQSFHTFPEQ-ELSLDG-IDYVTANKLRKM 512 Query: 516 LRKVVTGGTGKSLQGSKWPLAGKSGTAQTWVKGMPRNNQWFIGYGPVGHPRFAVSVLVEN 575 +RKVVT GTGK L S W +AGKSGTAQ N+ WF+GY P+ PR+A+ V+ EN Sbjct: 513 MRKVVTEGTGKVLMNSAWQVAGKSGTAQIG-DANGNNHLWFLGYAPLEEPRYAICVVAEN 571 Query: 576 VAPGSPHAATRLFGQVFDLLAESS 599 + A LF Q LA+ + Sbjct: 572 QPSWGKNQAMELFHQAVAGLADHT 595 >C5D4Y3.1 C5D4Y3_9BACI Penicillin-binding protein transpeptidase Length = 592 Score = 228 bits (580), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 185/586 (31%), Positives = 269/586 (45%), Gaps = 61/586 (10%) Query: 35 LLKDRKVPGAKYSMAQMAAIQSEREVVLDSGRGRLYDRSGQPLAGETVWTXXXXXXXXXX 94 L+ K ++ + + Q +E+V+D+GRG DR G+PL E + + Sbjct: 29 LISTESFSNKKVNLIEKSIEQRTQEMVIDNGRGTFIDRHGKPLTNEYIPSLVLFPFLKKM 88 Query: 95 XXXXXXXXGKEKLRQLATVLGVTYEQLQAERSGLKEPLLWPSGSGKNPLELTPGQAKAVE 154 + + +LA +L ++ + + KEP ++ P L Q + + Sbjct: 89 ---------EWPVEKLAEILHISPHIIIDQLEKAKEPFIFTIDG--TPFVLKEEQMEKIN 137 Query: 155 ALAVGGVRALPFARRYTGEATGRQWL-GYLSXXXXXXXXXSPTGLRVPWT--GTAGLERT 211 L + GV A+ +++ E T Q L G+ P P T G GL++ Sbjct: 138 QLHIPGVFAV--QKQHPLEKTYAQHLIGFTRTDTQLLRSRYPKRNLSPHTEVGIHGLQKA 195 Query: 212 LEPLMQGVGHTEAYAQVDARGSRLPDSPIKVRAPGNPYYPLSLYTTIDKKLQENIEKLAV 271 + + G T+ VDA G L +K PGNP+YP+S+ TTID+ LQ+ E + Sbjct: 196 FDEFLIPEGETKLLYHVDAEGGPLFGINVKYSDPGNPFYPVSVQTTIDRDLQQIAETIVR 255 Query: 272 KSGVKEGAVVVLDATTGDIEAMVSLPFYNPAQISPEGGEWNNRALQAAVPGSIFKIVTAA 331 K +K+G +V+LD T + AMVS P +P G N L PGSIFK V AA Sbjct: 256 KHHLKKGGIVLLDIETNSVLAMVSRPDMDPRNPYKNHGAENQMIL-PQTPGSIFKTVVAA 314 Query: 332 AALEAGVTSPEEKFYCSGHYGKYGLSCLNGKGHGSLTMAQGFAVSCNTVFASLAERXXXX 391 AALE G+ S + F CS G++ + +G L + FA SCN FA+L ++ Sbjct: 315 AALEKGMVSKQTTFDCSKKID--GVTPDDAHSYGMLGFEESFAASCNNAFATLGKKLVQQ 372 Query: 392 XXXXXXXXXXXXRDISWQAENTLGLPLLRPLSGEQPGAIFT-------------TLLPDD 438 DI LG L PLSG Q G ++ T+ D Sbjct: 373 DP-----------DILEIYAKKLG---LYPLSGWQ-GDVYHETNFEQFPEERKGTIWHDA 417 Query: 439 GGARV-----QTAIGQRDVRVTPLQAANLLVTLLHGGEVHAPRILQRVAFANGQTLKELP 493 R+ QT+IGQ+DVRV+PL AN++ T+ GE R + +V + NG T P Sbjct: 418 KDKRIPLAVAQTSIGQKDVRVSPLAVANMMATIARNGEAKQVRAVSKVLYKNGTTFFTFP 477 Query: 494 GHLAPSPQGRISPPTAHVLLSLLRKVVTG--GTGKSLQGSKWPLAGKSGTAQT--WVKGM 549 S + ISP TA L LLRKVV GTG+ Q + +AGKSGTA+T + Sbjct: 478 QQ-PFSDEQPISPVTASKLQQLLRKVVVDERGTGRRFQSLPYAVAGKSGTAETGKMLHNE 536 Query: 550 PRNNQWFIGYGPVGHPRFAVSVLVENVAPGSPHAATRLFGQVFDLL 595 N+WF GY P PR+A+ V+VE PGS +F +DL+ Sbjct: 537 ELINKWFAGYFPADAPRYAL-VVVELDCPGSQTVTNDVF---YDLV 578 >C2WCE9.1 C2WCE9_BACCE Putative uncharacterized protein Length = 528 Score = 213 bits (542), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 153/526 (29%), Positives = 242/526 (46%), Gaps = 42/526 (7%) Query: 62 LDSGRGRLYDRSGQPLAGETVWTXXXXXXXXXXXXXXXXXXGKEKLRQLATVLGVTYEQL 121 +D GRG DR+G+ L GE + + L +++ ++GV+ + + Sbjct: 1 MDDGRGHFVDRNGENL-GEERYPVLIIFPFLQIK--------NDMLEKISHIIGVSRQDI 51 Query: 122 QAERSGLKEPLLWPSGSGKNPLELTPGQAKAVEALAVGGVRALPFARRYTGEA------T 175 + ++P ++ G P +LT Q + V + G+ A + EA Sbjct: 52 NLQMKKKRKPFIFQRGD--KPFQLTKEQMEKVNRADMLGIVATEVRMKQPSEAEHLIGVV 109 Query: 176 GRQWLGYLSXXXXXXXXXSPTGLRVPWTGTAGLERTLEPLMQGVGHTEAYAQVDARGSRL 235 G + S T + G +GL+++ + + G T+ QVD +G + Sbjct: 110 GENEKEFKKRYEKLQKISSQTPV-----GISGLQQSFDEFLMTDGDTKVLYQVDRQGEPI 164 Query: 236 PDSPIKVRAPGNPYYPLSLYTTIDKKLQENIEKLAVKSGVKEGAVVVLDATTGDIEAMVS 295 K +PGNP+YP+++ TTI+K LQ+ E+L K G+K+G +V+LD +I A+VS Sbjct: 165 FGKRAKYTSPGNPFYPVTIQTTIEKSLQQKAEELVQKHGIKKGGLVLLDVKESEIVAIVS 224 Query: 296 LPFYNPAQISPEGGEWNNRALQAAVPGSIFKIVTAAAALEAGVTSPEEKFYCSGH-YGKY 354 P + N+ L PGS+FK V AAAA++ G+ P KF C YG+ Sbjct: 225 KPSLQMNNQNTYKKRMENQMLIPHFPGSVFKTVIAAAAIDNGMIQPNRKFNCDTDPYGEN 284 Query: 355 GLSCLNGKGHGSLTMAQGFAVSCNTVFASLAERXXXXXXXXXXXXXX---XXRDISWQAE 411 + GK L+ + FA SCN FA L R + W+ + Sbjct: 285 TPQVMMGK----LSFTESFARSCNRTFAVLGNELIQKDKDAIETYLEALGASRTVGWKGQ 340 Query: 412 NTLGLPLLRPLSGEQPGAIFTTLLPDDGGAR---VQTAIGQRDVRVTPLQAANLLVTLLH 468 P+ + L E+ ++ ++ G+ QTAIGQ+DVR++PL AN++ T+ Sbjct: 341 -VFHTPMFKQLVEEKQAIVWNE--EENKGSHKAIAQTAIGQKDVRISPLAVANMMATIAR 397 Query: 469 GGEVHAPRILQRVAFANGQTLKELPGHLAPSPQGRISPPTAHVLLSLLRKVVT--GGTGK 526 GE + ++ + + NG H Q +S T + SLLRKVVT GTG Sbjct: 398 NGEKREVKAVKEIRYKNGTKFFSFKDHKLEGQQ--LSYHTVKQVQSLLRKVVTMENGTGA 455 Query: 527 SLQGSKWPLAGKSGTAQTWVKGMPRNNQWFIGYGPVGHPRFAVSVL 572 Q +AGKSGTAQT + R N+WF GY P +PR+A+ V+ Sbjct: 456 GFQSLPLSVAGKSGTAQTGKED--RVNRWFAGYFPYENPRYALVVV 499 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,477,868,900 Number of extensions: 145915315 Number of successful extensions: 358462 Number of sequences better than 1.0e-05: 2467 Number of HSP's gapped: 356126 Number of HSP's successfully gapped: 2737 Length of query: 601 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 459 Effective length of database: 1,722,912,073 Effective search space: 790816641507 Effective search space used: 790816641507 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)