BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0020.p (361 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0K7Z0.1 B0K7Z0_THEP3 Transcriptional regulator, LacI family 117 2e-24 Q8RDH8.1 Q8RDH8_THETN Transcriptional regulator 115 6e-24 B7R8F5.1 B7R8F5_9THEO Periplasmic binding proteins and sugar bin... 115 6e-24 A4IT98.1 A4IT98_GEOTN Transcriptional regulator 110 2e-22 B4BR30.1 B4BR30_9BACI Transcriptional regulator, LacI family 110 2e-22 >B0K7Z0.1 B0K7Z0_THEP3 Transcriptional regulator, LacI family Length = 348 Score = 117 bits (293), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 89/369 (24%), Positives = 161/369 (43%), Gaps = 48/369 (13%) Query: 4 RKKVTLQHLATELGLTVHTVSKALRGLPGMSEYTRNEVLLLAQKLGYHTKEQERSLLFEN 63 RKKVT++ +A L L+++ VS AL G+SE TR VL A ++GY F+ Sbjct: 6 RKKVTMKDIAERLNLSINAVSLALNDKVGVSEETRKIVLKTADEMGY----------FDE 55 Query: 64 TPVYSGPS--RRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLF-------VPE 114 P + + R LI R + + ++ +++ + + + + F VP Sbjct: 56 NPSFIAKNHFRNICLLIEERNFRDTHFYTKVILGIENEAKKNNYDILVNFMNQDDFQVPS 115 Query: 115 GLQPAKAFTGWENKHNLSYADGIFISPVIEESVEAHLIQLKMPRILLNFPPPGANVDSVI 174 ++ K GI + I++ L++ +P +L++ + D+V+ Sbjct: 116 SVETRKV-------------SGILVVGTIKDEHLEKLLKYDIPTVLVDHASFTISTDAVL 162 Query: 175 WNVHDAMQQSARHLIAMGHRRIMYIGNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHIFE 234 + ++LI GH +I + G S ++ RW F + AGL V+P IF Sbjct: 163 TQNMPGTYMATQYLIRKGHTQIGFFGEIDFSLSFKERWLGFNEAMRNAGLNVDP---IFN 219 Query: 235 MN-----LPQEEWTALWKRQTEAYS--------PTAFICASEQALTRVYLACQTTGKHIP 281 +N + Q E L K E + PTA++C+++ A +Y A G +P Sbjct: 220 VNPGYCVIGQVEQYVLSKNYKEVANIISKLDKLPTAWVCSNDSAAITLYNALSILGIKVP 279 Query: 282 GDYSLMGLEPEPSLRNIIPDIARPTLPVKETGGRAADRMLWRIANPSLPYEHILLQGGLH 341 D S++G + + P + + + G +A R+LWR+ NP P +HI ++ L Sbjct: 280 DDISVVGFDDIDICNIVTPHLTTIRINKELMGEKAVKRLLWRMENPKEPCDHIRMEVKLI 339 Query: 342 NGSTVQPAH 350 +V+ + Sbjct: 340 ERESVKEIY 348 >Q8RDH8.1 Q8RDH8_THETN Transcriptional regulator Length = 352 Score = 115 bits (288), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 84/352 (23%), Positives = 161/352 (45%), Gaps = 26/352 (7%) Query: 4 RKKVTLQHLATELGLTVHTVSKALRGLPGMSEYTRNEVLLLAQKLGYHTKEQERSLLFEN 63 RK+VT++ +A +L L+++ VS AL G+SE TR V+ A+++GY FE Sbjct: 16 RKRVTMKDIAEKLNLSINAVSLALNDKVGVSEETRKLVIKTAEEMGY----------FEE 65 Query: 64 TPVYSGPS--RRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLFVPEGLQPAKA 121 P + S + +I R + + ++ +++ G+ V + F+ + L + Sbjct: 66 NPSFLSKSHLKNICLIIEERNFKDTNFYTKVIWGIENEAKANGYDVLVNFMKKDLFEIPS 125 Query: 122 FTGWENKHNLSYADGIFISPVIEESVEAHLIQLKMPRILLNFPPPGANVDSVIWNVHDAM 181 EN+ GI + I++ A ++ +P +L++ + D+V+ Sbjct: 126 CI--ENRK----VSGILVVGTIKDEFLAEILSYNIPTVLVDHASFMFSTDAVLTQNIPGS 179 Query: 182 QQSARHLIAMGHRRIMYIGNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHIFE------M 235 + ++LI GH++I + G S ++ RW F + AGL V D + E + Sbjct: 180 YRGTKYLIEKGHKKIGFFGEKDFSLSFRERWLGFKEAMRDAGLPVNEDYCVTEDVERHVL 239 Query: 236 NLPQEEWTALWKRQTEAYSPTAFICASEQALTRVYLACQTTGKHIPGDYSLMGLEPEPSL 295 + EE K+ E PTA++C+++ +Y A G +P D S++G + Sbjct: 240 SKNYEEVANRLKKLKEF--PTAWVCSNDSNAITLYNALNIMGIKVPKDVSIVGFDDIDMC 297 Query: 296 RNIIPDIARPTLPVKETGGRAADRMLWRIANPSLPYEHILLQGGLHNGSTVQ 347 + P + + + G +A R+LWR+ NP ++HI ++ G +V+ Sbjct: 298 NIVTPPLTTIHINKELMGIKAVKRLLWRMDNPKEFHDHIRMEVGFVERQSVR 349 >B7R8F5.1 B7R8F5_9THEO Periplasmic binding proteins and sugar binding domain of the LacI family, putative Length = 352 Score = 115 bits (288), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 84/352 (23%), Positives = 161/352 (45%), Gaps = 26/352 (7%) Query: 4 RKKVTLQHLATELGLTVHTVSKALRGLPGMSEYTRNEVLLLAQKLGYHTKEQERSLLFEN 63 RK+VT++ +A +L L+++ VS AL G+SE TR V+ A+++GY FE Sbjct: 16 RKRVTMKDIAEKLNLSINAVSLALNDKVGVSEETRKLVIKTAEEMGY----------FEE 65 Query: 64 TPVYSGPS--RRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLFVPEGLQPAKA 121 P + S + +I R + + ++ +++ G+ V + F+ + L + Sbjct: 66 NPSFLSKSHLKNICLIIEERNFKDTNFYTKVIWGIENEAKANGYDVLVNFMKKDLFEIPS 125 Query: 122 FTGWENKHNLSYADGIFISPVIEESVEAHLIQLKMPRILLNFPPPGANVDSVIWNVHDAM 181 EN+ GI + I++ A ++ +P +L++ + D+V+ Sbjct: 126 CI--ENRK----VSGILVVGTIKDEFLAEILSYNIPTVLVDHASFMFSTDAVLTQNIPGS 179 Query: 182 QQSARHLIAMGHRRIMYIGNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHIFE------M 235 + ++LI GH++I + G S ++ RW F + AGL V D + E + Sbjct: 180 YRGTKYLIEKGHKKIGFFGEKDFSLSFRERWLGFKEAMRDAGLPVNEDYCVTEDVERHVL 239 Query: 236 NLPQEEWTALWKRQTEAYSPTAFICASEQALTRVYLACQTTGKHIPGDYSLMGLEPEPSL 295 + EE K+ E PTA++C+++ +Y A G +P D S++G + Sbjct: 240 SKNYEEVANRLKKLKEF--PTAWVCSNDSNAITLYNALNIMGIKVPKDVSIVGFDDIDMC 297 Query: 296 RNIIPDIARPTLPVKETGGRAADRMLWRIANPSLPYEHILLQGGLHNGSTVQ 347 + P + + + G +A R+LWR+ NP ++HI ++ G +V+ Sbjct: 298 NIVTPPLTTIHINKELMGIKAVKRLLWRMDNPKEFHDHIRMEVGFVERQSVR 349 >A4IT98.1 A4IT98_GEOTN Transcriptional regulator Length = 337 Score = 110 bits (274), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 85/348 (24%), Positives = 154/348 (44%), Gaps = 33/348 (9%) Query: 3 IRKKVTLQHLATELGLTVHTVSKALRGLPGMSEYTRNEVLLLAQKLGYHTKEQERSLLFE 62 ++K+VT+Q +A L ++ ++VS+ALRG G+SE TR V +A+++GY RS E Sbjct: 1 MKKRVTMQDIADRLNISKNSVSQALRGKEGVSEETRQLVKRVAKEMGYEYPSS-RSKHRE 59 Query: 63 NTPVYSGPSRRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLFVPEGLQPAKAF 122 P + I LIA+ + + S++ G K+ + V E + + Sbjct: 60 EPP-------KQIALIASDRAFSLKFFGEIYLSIEQEALSHGMKLHIQSVNEQQKQQRLL 112 Query: 123 TGWENKHNLSYADGIFISPVIEESVEAHLIQLKMPRILLNFPPPGANVDSVIWNVHDAMQ 182 + + DG+ I I ++ +P +L++ P + D+V+ N Sbjct: 113 PSFIEE---KLVDGVIILSHISTEYIQKVVSTGIPTVLVDHHHPNISADAVLTNNRFGAY 169 Query: 183 QSARHLIAMGHRRIMYIGNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHIFEMNLPQEEW 242 + +HL+ + H I ++G+ S YQ R++ + + L+ G++ P EEW Sbjct: 170 IAVQHLLELNHTDIAFVGDIHYSPSYQERYEGYLLALKDYGVK------------PNEEW 217 Query: 243 TALWKRQTEA----------YSPTAFICASEQALTRVYLACQTTGKHIPGDYSLMGLEPE 292 ++ E PTA+ C ++ V + Q G ++PGD S+ + Sbjct: 218 IFCHAQEDETAIANYIRELKQQPTAWFCVNDGLGFFVSTSLQQYGLNVPGDVSVCSFDNG 277 Query: 293 PSLRNIIPDIARPTLPVKETGGRAADRMLWRIANPSLPYEHILLQGGL 340 + P I + +K G RA + + WR NP P++ +LL L Sbjct: 278 QLSQIATPKITTVDIDLKRYGKRAVELLRWRWDNPDEPFQEVLLSTKL 325 >B4BR30.1 B4BR30_9BACI Transcriptional regulator, LacI family Length = 337 Score = 110 bits (274), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 85/348 (24%), Positives = 154/348 (44%), Gaps = 33/348 (9%) Query: 3 IRKKVTLQHLATELGLTVHTVSKALRGLPGMSEYTRNEVLLLAQKLGYHTKEQERSLLFE 62 ++K+VT+Q +A L ++ ++VS+ALRG G+SE TR V +A+++GY RS E Sbjct: 1 MKKRVTMQDIADRLNISKNSVSQALRGKEGVSEETRQLVKRVAKEMGYEYPSS-RSKHRE 59 Query: 63 NTPVYSGPSRRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLFVPEGLQPAKAF 122 P + I LIA+ + + S++ G K+ + V E + + Sbjct: 60 EPP-------KQIALIASDRAFSLKFFGEIYLSIEQEALSHGMKLHIQSVNEQQKQQRLL 112 Query: 123 TGWENKHNLSYADGIFISPVIEESVEAHLIQLKMPRILLNFPPPGANVDSVIWNVHDAMQ 182 + + DG+ I I ++ +P +L++ P + D+V+ N Sbjct: 113 PSFIEE---KLVDGVIILSHISTEYIQKVVSTGIPTVLVDHHHPNISADAVLTNNRFGAY 169 Query: 183 QSARHLIAMGHRRIMYIGNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHIFEMNLPQEEW 242 + +HL+ + H I ++G+ S YQ R++ + + L+ G++ P EEW Sbjct: 170 IAVQHLLELNHTDIAFVGDIHYSPSYQERYEGYLLALKDYGVK------------PNEEW 217 Query: 243 TALWKRQTEA----------YSPTAFICASEQALTRVYLACQTTGKHIPGDYSLMGLEPE 292 ++ E PTA+ C ++ V + Q G ++PGD S+ + Sbjct: 218 IFCHAQEDETAIANYIRELKQQPTAWFCVNDGLGFFVSTSLQQYGLNVPGDVSVCSFDNG 277 Query: 293 PSLRNIIPDIARPTLPVKETGGRAADRMLWRIANPSLPYEHILLQGGL 340 + P I + +K G RA + + WR NP P++ +LL L Sbjct: 278 QLSQIATPKITTVDIDLKRYGKRAVELLRWRWDNPDEPFQEVLLSTKL 325 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,317,426,227 Number of extensions: 100059671 Number of successful extensions: 219375 Number of sequences better than 1.0e-05: 1423 Number of HSP's gapped: 220123 Number of HSP's successfully gapped: 1423 Length of query: 361 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 224 Effective length of database: 1,770,017,148 Effective search space: 396483841152 Effective search space used: 396483841152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)