BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0017.p (406 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DML8.1 B1DML8_9BACL N-acylglucosamine 2-epimerase 468 e-130 A9AYF3.1 A9AYF3_HERA2 N-acylglucosamine 2-epimerase 398 e-109 B1ZQI2.1 B1ZQI2_OPITP N-acylglucosamine 2-epimerase 398 e-109 A6E7V4.1 A6E7V4_9SPHI Putative N-acyl-D-glucosamine 2-epimerase 389 e-106 A5FA14.1 A5FA14_FLAJ1 N-acylglucosamine 2-epimerase 381 e-104 >B1DML8.1 B1DML8_9BACL N-acylglucosamine 2-epimerase Length = 408 Score = 468 bits (1203), Expect = e-130, Method: Compositional matrix adjust. Identities = 226/395 (57%), Positives = 281/395 (71%) Query: 2 KISTEEWRRQLENELKNNILGFWMEHTLDEAHGGFAGEIDNQMNVIADAEKSLVLNARIL 61 ++ W+ ++E+ELK+NIL FWM+H+LDE GGF G++ N + V A+KSLVLNAR+L Sbjct: 6 QLDLRRWQMEMESELKDNILSFWMKHSLDERDGGFVGQMTNDLQVNETADKSLVLNARLL 65 Query: 62 WTFASAYRLYGTSEYLAMAERAYDYLMQHFTDTEYGGLFWMVDARGVPSQPKKQIYGQAF 121 WT+ASAYR Y YL MAERAY YL+ +F D GG +W+++ +G Q KKQIYGQAF Sbjct: 66 WTYASAYRKYREPGYLRMAERAYHYLISNFQDRVNGGYYWLLNDKGEQIQTKKQIYGQAF 125 Query: 122 AIYALAEFYHATGRADALQQAVDLFHIVEKHGYDPIYKGYIEALSREWQGTDNLSLSSKD 181 IYAL+EF A LQ+AV LF ++E++ YD +KGYIEAL W+ T++LSLS+ D Sbjct: 126 VIYALSEFVRACPDEKVLQEAVRLFGLIERYSYDSKHKGYIEALGSNWEATEDLSLSNTD 185 Query: 182 MNEKKSMNTHLHVLEGYTGLYRVWKSEELKSKLAELIETMLDHIIDADGKHFYLFLDEEW 241 +NEKKSMNTHLHVLE YT LYRVW SE+LK K ELI TMLDHI+D HF LF DE W Sbjct: 186 LNEKKSMNTHLHVLEAYTNLYRVWPSEQLKEKQKELIGTMLDHIVDLQSGHFKLFFDERW 245 Query: 242 HVKSQSISYGHDIEGSWLLVEAAEVLGDEALLQRVRKVAVSMAEAVLAEGIAEDGGIWNE 301 + KS ISYGHDIEGSWLL+EAAEVL DE ++ R +A++MA+AV EG+ EDG + NE Sbjct: 246 NSKSDEISYGHDIEGSWLLMEAAEVLADEEVISRTETIAIAMAQAVYEEGVDEDGAVVNE 305 Query: 302 ADGSGHIDKSRDWWPQAEAVVGFYNAYQLTHETRFLEAAENAWIFTDRYIVDHKLGEWYW 361 AD G D + WWPQAEAVVGF NAYQ T + FL+AA W F DRYIVD GEW+W Sbjct: 306 ADPQGWTDTDKIWWPQAEAVVGFLNAYQHTGKKHFLQAAYRTWNFIDRYIVDKTNGEWFW 365 Query: 362 GVDEALQPLAREPKVSAWKCPYHNSRACFEMIGRL 396 V+ A +P E K+ WKCPYHN R E++ RL Sbjct: 366 KVNRAGEPGIAEMKIDPWKCPYHNGRMGLEVLERL 400 >A9AYF3.1 A9AYF3_HERA2 N-acylglucosamine 2-epimerase Length = 416 Score = 398 bits (1022), Expect = e-109, Method: Compositional matrix adjust. Identities = 196/394 (49%), Positives = 249/394 (63%) Query: 6 EEWRRQLENELKNNILGFWMEHTLDEAHGGFAGEIDNQMNVIADAEKSLVLNARILWTFA 65 E W+ Q +E+ NIL FW HTLD HGGF G + N + + + +S VL RILWTFA Sbjct: 15 EMWQAQFRDEVLGNILPFWANHTLDHEHGGFYGGLTNDLTIHNEFPRSAVLCGRILWTFA 74 Query: 66 SAYRLYGTSEYLAMAERAYDYLMQHFTDTEYGGLFWMVDARGVPSQPKKQIYGQAFAIYA 125 AYR+ G LA+AE AY YL Q F D YGGL+W +DA G P KQ Y Q+FAIY Sbjct: 75 CAYRMLGDPRDLAVAEYAYAYLKQAFWDQTYGGLYWSIDANGQPLADHKQTYAQSFAIYG 134 Query: 126 LAEFYHATGRADALQQAVDLFHIVEKHGYDPIYKGYIEALSREWQGTDNLSLSSKDMNEK 185 LAE+ ATG AL+ A LFH++E H +D +Y GYIE R WQ + LS + + Sbjct: 135 LAEYVRATGDQSALELAQTLFHLIENHAFDAVYGGYIEGCDRVWQPLGDSRLSKLEPEAR 194 Query: 186 KSMNTHLHVLEGYTGLYRVWKSEELKSKLAELIETMLDHIIDADGKHFYLFLDEEWHVKS 245 K+MNT LH++E Y L RVW +++ +LA LIE +HIID F+LF D++W+ Sbjct: 195 KTMNTMLHMMEAYANLLRVWDVADVRQQLASLIEACCEHIIDPVQGRFHLFFDDQWNHHE 254 Query: 246 QSISYGHDIEGSWLLVEAAEVLGDEALLQRVRKVAVSMAEAVLAEGIAEDGGIWNEADGS 305 ISYGHDIEGSWLL+EAA VLGDE L+ + +A+ MA+AV G DG I +E Sbjct: 255 HGISYGHDIEGSWLLMEAAHVLGDEHLIAKAETLAIGMADAVYRNGRHADGSIIHERAPD 314 Query: 306 GHIDKSRDWWPQAEAVVGFYNAYQLTHETRFLEAAENAWIFTDRYIVDHKLGEWYWGVDE 365 G I+ R WWPQAEAVVGFYNAYQ T + F +AA ++W F RY +DH G+W+ +D Sbjct: 315 GSINLERHWWPQAEAVVGFYNAYQATGKPEFAQAAYDSWNFIQRYFIDHDHGDWFKILDA 374 Query: 366 ALQPLAREPKVSAWKCPYHNSRACFEMIGRLTLH 399 QPL PKV W+CPYH++R CFEMI RL H Sbjct: 375 QNQPLGAIPKVGPWECPYHHARVCFEMIERLAEH 408 >B1ZQI2.1 B1ZQI2_OPITP N-acylglucosamine 2-epimerase Length = 403 Score = 398 bits (1022), Expect = e-109, Method: Compositional matrix adjust. Identities = 188/386 (48%), Positives = 255/386 (66%) Query: 11 QLENELKNNILGFWMEHTLDEAHGGFAGEIDNQMNVIADAEKSLVLNARILWTFASAYRL 70 ++E++L++NIL FW+ H +D G F G + N++ V +AE+ +L RILWTF++AY Sbjct: 12 RIESDLRSNILAFWIRHVVDHERGTFFGALTNELTVDCNAERGALLTCRILWTFSAAYHR 71 Query: 71 YGTSEYLAMAERAYDYLMQHFTDTEYGGLFWMVDARGVPSQPKKQIYGQAFAIYALAEFY 130 YG S+Y AMA+RAY L+ +F D + GG +W + A G + +KQ+YGQAFAIYALAE+Y Sbjct: 72 YGDSDYRAMADRAYADLLTNFADGDCGGFYWSIAADGRVLRDRKQVYGQAFAIYALAEYY 131 Query: 131 HATGRADALQQAVDLFHIVEKHGYDPIYKGYIEALSREWQGTDNLSLSSKDMNEKKSMNT 190 +TG AL A+ +F ++EK+ D Y GY+EA SR WQ ++ LS D+NE KS NT Sbjct: 132 RSTGEPAALHTAIAVFRLLEKYARDSNYGGYLEAFSRSWQPIADMRLSDVDLNEPKSQNT 191 Query: 191 HLHVLEGYTGLYRVWKSEELKSKLAELIETMLDHIIDADGKHFYLFLDEEWHVKSQSISY 250 HLHV+E YT L RVW L+ L+ETMLD I++ + H LF +W ++S ISY Sbjct: 192 HLHVMEAYTNLLRVWPDASLRRAQTRLLETMLDRIVNPETCHLGLFFAHDWSLRSDKISY 251 Query: 251 GHDIEGSWLLVEAAEVLGDEALLQRVRKVAVSMAEAVLAEGIAEDGGIWNEADGSGHIDK 310 GHDIE +WL EAAEVLG+ +L R+ +AV +A+ LAEG+ DGGI+NE G D Sbjct: 252 GHDIEAAWLFTEAAEVLGNSSLTARIHPLAVRIADVTLAEGVDADGGIYNEGGPRGLTDT 311 Query: 311 SRDWWPQAEAVVGFYNAYQLTHETRFLEAAENAWIFTDRYIVDHKLGEWYWGVDEALQPL 370 +++WWPQAEAVVGF NAY L+H+ R L+AA W F D+ ++D + GEW GV + L Sbjct: 312 NKEWWPQAEAVVGFLNAYALSHDERHLQAALRCWDFIDQRLIDKQRGEWLRGVTRDGKVL 371 Query: 371 AREPKVSAWKCPYHNSRACFEMIGRL 396 A + KVS WKCPYHN R E RL Sbjct: 372 ADQLKVSFWKCPYHNGRTGLEAPARL 397 >A6E7V4.1 A6E7V4_9SPHI Putative N-acyl-D-glucosamine 2-epimerase Length = 399 Score = 389 bits (999), Expect = e-106, Method: Compositional matrix adjust. Identities = 186/399 (46%), Positives = 270/399 (67%), Gaps = 3/399 (0%) Query: 1 MKISTEEWRRQLENELKNNILGFWMEHTLDEAHGGFAGEIDNQMNVIADAEKSLVLNARI 60 MK ++R +L +EL+N IL +W +H DE +GGF G+ID+ ++ A K VLNARI Sbjct: 1 MKPDLLKYREELSSELEN-ILSYWSKHVPDEINGGFVGKIDHNGDIDHAAPKGSVLNARI 59 Query: 61 LWTFASAYRLYGTSEYLAMAERAYDYLMQHFTDTEYGGLFWMVDARGVPSQPKKQIYGQA 120 LW+F++AYR Y+ +A+RA++Y++ +F D +YGG++W +DA G P KKQ+Y A Sbjct: 60 LWSFSAAYRCDPDERYIELADRAFNYIIDYFIDEQYGGVYWSLDASGWPLDGKKQVYAIA 119 Query: 121 FAIYALAEFYHATGRADALQQAVDLFHIVEKHGYDPIYKGYIEALSREWQGTDNLSLSSK 180 F IYAL+E+Y + A Q+A+ L+ + ++ YDP+ GY EA +R+W ++L LS K Sbjct: 120 FTIYALSEYYQVSLNEQARQKAMALYQTLMEYSYDPVNGGYFEAFNRDWSDLEDLRLSEK 179 Query: 181 DMNEKKSMNTHLHVLEGYTGLYRVWKSEELKSKLAELIETMLDHIIDADGKHFYLFLDEE 240 D NEKK+MNTHLHVLE +T LYR+W + LK ++ LI DH+I+ + + LF DE+ Sbjct: 180 DANEKKTMNTHLHVLEAFTTLYRIWPDDGLKDRITALIHNFTDHMINEETGNLILFFDED 239 Query: 241 WHVKSQSISYGHDIEGSWLLVEAAEVLGDEALLQRVRKVAVSMAEAVLAEGIAEDGGIWN 300 W+ KS ISYGHDIE +WLL+EAAEV+ E L++ +R A+ MAE V +G+ DGG+W Sbjct: 240 WNAKSDLISYGHDIEAAWLLLEAAEVIQHEELVRLLRDNALKMAE-VSKKGLDVDGGLWY 298 Query: 301 E-ADGSGHIDKSRDWWPQAEAVVGFYNAYQLTHETRFLEAAENAWIFTDRYIVDHKLGEW 359 E A H+ + + WW QAEA+VGFYNA+Q++ + FL+ + W F +++DH+ GEW Sbjct: 299 EYAPSDHHLIREKHWWVQAEAMVGFYNAWQISDDPEFLDISLKIWTFVQAHLIDHEKGEW 358 Query: 360 YWGVDEALQPLAREPKVSAWKCPYHNSRACFEMIGRLTL 398 +WGV E + + E KV WKCPYHNSRAC E+I R++L Sbjct: 359 FWGVTETGEVMPNEDKVGMWKCPYHNSRACIELIKRISL 397 >A5FA14.1 A5FA14_FLAJ1 N-acylglucosamine 2-epimerase Length = 396 Score = 381 bits (978), Expect = e-104, Method: Compositional matrix adjust. Identities = 184/391 (47%), Positives = 260/391 (66%), Gaps = 6/391 (1%) Query: 10 RQLENELK---NNILGFWMEHTLDEAHGGFAGEIDNQMNVIADAEKSLVLNARILWTFAS 66 +QL++EL ++IL +W +HTLD + GF G+ID +++A+AEK VLN+RILWTF+S Sbjct: 6 KQLKSELTAELDSILNYWSKHTLDNQNEGFVGQIDFNDHIVANAEKGSVLNSRILWTFSS 65 Query: 67 AYRLYGTSEYLAMAERAYDYLMQHFTDTEYGGLFWMVDARGVPSQPKKQIYGQAFAIYAL 126 +Y++ + +A+RA++YL ++F D E+ GLFW ++A P K QIY AFAIY L Sbjct: 66 SYQITKKENHKEIAKRAFEYLSKYFYDPEFNGLFWSINADKTPKDTKNQIYALAFAIYGL 125 Query: 127 AEFYHATGRADALQQAVDLFHIVEKHGYDPIYKGYIEALSREWQGTDNLSLSSKDMNEKK 186 +E+Y + AL+ AV+L+ ++K+ YDP+ KGY+EA +R WQ ++L LS KD NEKK Sbjct: 126 SEYYVISQDQKALETAVNLYKSIQKYSYDPVNKGYLEAFTRNWQPIEDLRLSDKDANEKK 185 Query: 187 SMNTHLHVLEGYTGLYRVWKSEELKSKLAELIETMLDHIIDADGKHFYLFLDEEWHVKSQ 246 +MNTHLH++E Y L++VWK E LK EL+ET+ H I+ + H LF DE W K Sbjct: 186 TMNTHLHIIEAYANLFKVWKDETLKKDSIELLETIEKHFINTETGHLRLFFDENWIEKPD 245 Query: 247 SISYGHDIEGSWLLVEAAEVLGDEALLQRVRKVAVSMAEAVLAEGIAEDGGIWNEADGS- 305 ISYGHDIE +WLL++ AEVL +E L+ +K AV +AE V EG DGG+W E + Sbjct: 246 VISYGHDIEAAWLLLQCAEVLEEENLIANYKKHAVQIAE-VTKEGFDTDGGLWYEFEPKE 304 Query: 306 GHIDKSRDWWPQAEAVVGFYNAYQLTHETRFLEAAENAWIFTDRYIVDHKLGEWYWGVDE 365 + + WW QAEA++GFYNAYQLT +L+ W F ++I+DHK GEW+WG+ + Sbjct: 305 NELIAEKHWWVQAEALIGFYNAYQLTGNEEYLKIVFENWNFIKKHILDHKNGEWFWGIHK 364 Query: 366 ALQPLAREPKVSAWKCPYHNSRACFEMIGRL 396 L ++ K WKCPYHNSRAC E+I R+ Sbjct: 365 DY-SLIQKDKAGFWKCPYHNSRACLELINRI 394 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,544,292,405 Number of extensions: 105513912 Number of successful extensions: 267808 Number of sequences better than 1.0e-05: 283 Number of HSP's gapped: 267257 Number of HSP's successfully gapped: 292 Length of query: 406 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 268 Effective length of database: 1,760,596,133 Effective search space: 471839763644 Effective search space used: 471839763644 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)