BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0014.p (354 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAQ7.1 B3KAQ7_9BACI Periplasmic binding protein/LacI transcrip... 369 e-100 B1DGV9.1 B1DGV9_9BACL Transcriptional regulator, LacI family 333 1e-89 B3K9B8.1 B3K9B8_9BACI Periplasmic binding protein/LacI transcrip... 266 2e-69 Q8RDH8.1 Q8RDH8_THETN Transcriptional regulator 176 3e-42 B7R8F5.1 B7R8F5_9THEO Periplasmic binding proteins and sugar bin... 176 3e-42 >B3KAQ7.1 B3KAQ7_9BACI Periplasmic binding protein/LacI transcriptional regulator Length = 352 Score = 369 bits (947), Expect = e-100, Method: Compositional matrix adjust. Identities = 179/354 (50%), Positives = 247/354 (69%), Gaps = 4/354 (1%) Query: 1 VAKKVTMQKIADHLGVSKFVVSKSLSGKGGVNETTRERVIQAASQLGYFTQKNAYVQNVK 60 +A KVTMQ+IADHLG+SKF VSK+LSGK GV+ TRE+++ A+QLGYF K+ + K Sbjct: 1 MAGKVTMQQIADHLGLSKFAVSKALSGKSGVSLETREKIVNVATQLGYFANKSQKAGSKK 60 Query: 61 RPSPAGGSDRNKQSVLVLMPNIRSQTQDSLYWGKIVDGIALALDQVGLGMVIVSEHRADN 120 + A GS K++V+VL+PN+R QT+DSLYWG+I+DGI L+ GMVIV++ D+ Sbjct: 61 NEASADGSQAAKETVIVLVPNVRYQTRDSLYWGRIIDGITNELESRDTGMVIVTDQFKDS 120 Query: 121 FINILNPSGLLGLVGVGQISTSLLLEVHRIGLPMVLIDHEDPLIPSDTVFANNIDSMTRL 180 F N++NPSG+ G++GVG IS+ LLLE+ + +P VLIDHEDPLIPSD +F NN++ M R+ Sbjct: 121 FANLINPSGVRGIIGVGFISSQLLLEIRSLDIPFVLIDHEDPLIPSDALFMNNVECMRRV 180 Query: 181 SNHLMGIGHTLLHFIGNIRYSRSFRDRWIGFRSALEENDMKTPAGDDEMLVLEGMEDGTF 240 +N+L+G GH L F+GN+R+SRSF DR+ G+RS L E + ++E+L EG Sbjct: 181 TNYLIGCGHRTLQFVGNVRFSRSFADRYQGYRSVLVEQGIPLDQ-NEELLSFEGDNRSEM 239 Query: 241 RDDFRLWLAKRKKAKTIPSALVCANDSTALIVCEVLREEGVAIPADISVTGFDNIEDAMR 300 + L++ ++ +P+A VCANDS A+ + VL + G +PAD SVTGFDNIEDA Sbjct: 240 TEALETILSRIEE---LPTAFVCANDSIAICIMTVLGKLGAKVPADCSVTGFDNIEDAAY 296 Query: 301 GIPPLTTVHVPKEAMGRTAVEKLMNRIQNPSAPLEKILIAADIVHRDSVAGPGK 354 P L+T+HV KEA GR AVE L R++ P +PLEKIL+A D + R+S A K Sbjct: 297 AAPSLSTIHVNKEAFGRRAVEMLARRVEQPDSPLEKILLAGDFILRESTAYAKK 350 >B1DGV9.1 B1DGV9_9BACL Transcriptional regulator, LacI family Length = 353 Score = 333 bits (854), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 160/349 (45%), Positives = 237/349 (67%), Gaps = 2/349 (0%) Query: 4 KVTMQKIADHLGVSKFVVSKSLSGKGGVNETTRERVIQAASQLGYFTQKNAYVQNVKRPS 63 KVTMQ+IADHLGVSKF VSK+LSGK GV+ TR++++ A+QLGYF K KR Sbjct: 4 KVTMQQIADHLGVSKFAVSKALSGKPGVSAETRDKIVSVATQLGYFVNKQTNTA-AKRNK 62 Query: 64 PAGGSDRNKQSVLVLMPNIRSQTQDSLYWGKIVDGIALALDQVGLGMVIVSEHRADNFIN 123 PA + +V++L+P +R QT+DS +WG+I+DG+ L + ++V+E+ DNF N Sbjct: 63 PAPFITNTRDTVILLVPKVRFQTRDSYFWGRIIDGVTEELADRDISALVVTENFKDNFSN 122 Query: 124 ILNPSGLLGLVGVGQISTSLLLEVHRIGLPMVLIDHEDPLIPSDTVFANNIDSMTRLSNH 183 ++NP+G+ G++GVG I++ +LLE+ +G+P VL+DHEDPLIPSD +F NN++ R++N+ Sbjct: 123 LINPNGVSGIIGVGYIASKMLLEIRNLGIPFVLVDHEDPLIPSDVLFMNNMECTRRVTNY 182 Query: 184 LMGIGHTLLHFIGNIRYSRSFRDRWIGFRSALEENDMKTPAGDDEMLVLEGMEDGTFRDD 243 L+G GH+ L F+GN RYSRSF DR+IG+R+ +EE+ + ++ +L EG + Sbjct: 183 LIGCGHSKLQFVGNTRYSRSFVDRYIGYRTMMEEHGLALNQ-EERLLSFEGGNRSEITEA 241 Query: 244 FRLWLAKRKKAKTIPSALVCANDSTALIVCEVLREEGVAIPADISVTGFDNIEDAMRGIP 303 + + + +P+A VCANDS A+ + VL + V +P SVTGFDNIEDA P Sbjct: 242 LEVIVKELINEHRLPTAFVCANDSIAICMMTVLGKLSVQVPGQCSVTGFDNIEDAAWAKP 301 Query: 304 PLTTVHVPKEAMGRTAVEKLMNRIQNPSAPLEKILIAADIVHRDSVAGP 352 L+TV+V KEA+GR AVE L+ R+++P + EKIL+A + + R+S A P Sbjct: 302 TLSTVNVQKEAIGRRAVEMLLRRLEHPDSLQEKILLAGEFIIRESTALP 350 >B3K9B8.1 B3K9B8_9BACI Periplasmic binding protein/LacI transcriptional regulator Length = 347 Score = 266 bits (681), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 143/356 (40%), Positives = 214/356 (60%), Gaps = 33/356 (9%) Query: 4 KVTMQKIADHLGVSKFVVSKSLSGKGGVNETTRERVIQAASQLGYFTQKNAYVQNVKRPS 63 KVT+Q+IAD GVSKF VS++LSGK GV+ TR+ +++AA QLGYF + Sbjct: 4 KVTIQEIADFTGVSKFAVSRALSGKSGVSPQTRDMILKAAGQLGYF-----------KDQ 52 Query: 64 PAGGSDRNKQ-----------SVLVLMPNIRSQTQDSLYWGKIVDGIALALDQVGLGMVI 112 P + RN+Q +VLVL PN+R Q ++SLYWG + DGI+ L+Q GL ++ Sbjct: 53 PGQAAGRNQQLQEPENRKWAGAVLVLFPNVRYQNRESLYWGPVFDGISTRLNQKGLDILT 112 Query: 113 VSEHRADNFINILNPSGLLGLVGVGQISTSLLLEVHRIGLPMVLIDHEDPLIPSDTVFAN 172 ++E D+ ++LNP G+LG+V VG +ST L+++ ++ +P+V++DH DP SDTVF + Sbjct: 113 LTEPSNDHMFSLLNPQGILGIVTVGAVSTQNLMDIKKLNIPVVMVDHMDPAFQSDTVFTD 172 Query: 173 NIDSMTRLSNHLMGIGHTLLHFIGNIRYSRSFRDRWIGFRSALEENDMKTPAGDDEMLVL 232 N+ SM ++ L+ G+ FIGNI ++SF +RWIGF SAL D K L+ Sbjct: 173 NMHSMKQIMTKLISKGYKKYQFIGNIEDAQSFYERWIGFSSALA--DYKIEHRQLPSLIS 230 Query: 233 EGMEDGTFRDDFRLWLAKRKKAKTIPSALVCANDSTALIVCEVLREEGVAIPADISVTGF 292 +E + L K +P VCAND TA+ E L +G+ +P + VTGF Sbjct: 231 PEIE------NIHETLGKALAGNELPEVFVCANDITAMFAMEALGNQGIDVPGHVQVTGF 284 Query: 293 DNIEDAMRGIPPLTTVHVPKEAMGRTAVEKLMNRIQNPSAPLEKILIAADIVHRDS 348 DN D++ P + TV++ KE +G AV++L+ RI NP + E++LI AD++ R+S Sbjct: 285 DNTYDSL---PIMATVNIDKELLGMRAVDQLLWRIVNPDSNYERLLIQADVIVRES 337 >Q8RDH8.1 Q8RDH8_THETN Transcriptional regulator Length = 352 Score = 176 bits (446), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 111/349 (31%), Positives = 183/349 (52%), Gaps = 19/349 (5%) Query: 3 KKVTMQKIADHLGVSKFVVSKSLSGKGGVNETTRERVIQAASQLGYFTQKNAYVQNVKRP 62 K+VTM+ IA+ L +S VS +L+ K GV+E TR+ VI+ A ++GYF + Sbjct: 17 KRVTMKDIAEKLNLSINAVSLALNDKVGVSEETRKLVIKTAEEMGYFEE----------- 65 Query: 63 SPAGGSDRNKQSVLVLMPNIRSQTQDSLYWGKIVDGIALALDQVGLGMVIVSEHRADNFI 122 +P+ S + +++ +++ +D+ ++ K++ GI G V+V+ + D F Sbjct: 66 NPSFLSKSHLKNICLIIE--ERNFKDTNFYTKVIWGIENEAKANGYD-VLVNFMKKDLFE 122 Query: 123 --NILNPSGLLGLVGVGQISTSLLLEVHRIGLPMVLIDHEDPLIPSDTVFANNIDSMTRL 180 + + + G++ VG I L E+ +P VL+DH + +D V NI R Sbjct: 123 IPSCIENRKVSGILVVGTIKDEFLAEILSYNIPTVLVDHASFMFSTDAVLTQNIPGSYRG 182 Query: 181 SNHLMGIGHTLLHFIGNIRYSRSFRDRWIGFRSALEENDMKTPAGDDEMLVLEGMEDGTF 240 + +L+ GH + F G +S SFR+RW+GF+ A+ D P +D V E +E Sbjct: 183 TKYLIEKGHKKIGFFGEKDFSLSFRERWLGFKEAMR--DAGLPVNED-YCVTEDVERHVL 239 Query: 241 RDDFRLWLAKRKKAKTIPSALVCANDSTALIVCEVLREEGVAIPADISVTGFDNIEDAMR 300 ++ + KK K P+A VC+NDS A+ + L G+ +P D+S+ GFD+I+ Sbjct: 240 SKNYEEVANRLKKLKEFPTAWVCSNDSNAITLYNALNIMGIKVPKDVSIVGFDDIDMCNI 299 Query: 301 GIPPLTTVHVPKEAMGRTAVEKLMNRIQNPSAPLEKILIAADIVHRDSV 349 PPLTT+H+ KE MG AV++L+ R+ NP + I + V R SV Sbjct: 300 VTPPLTTIHINKELMGIKAVKRLLWRMDNPKEFHDHIRMEVGFVERQSV 348 >B7R8F5.1 B7R8F5_9THEO Periplasmic binding proteins and sugar binding domain of the LacI family, putative Length = 352 Score = 176 bits (446), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 111/349 (31%), Positives = 183/349 (52%), Gaps = 19/349 (5%) Query: 3 KKVTMQKIADHLGVSKFVVSKSLSGKGGVNETTRERVIQAASQLGYFTQKNAYVQNVKRP 62 K+VTM+ IA+ L +S VS +L+ K GV+E TR+ VI+ A ++GYF + Sbjct: 17 KRVTMKDIAEKLNLSINAVSLALNDKVGVSEETRKLVIKTAEEMGYFEE----------- 65 Query: 63 SPAGGSDRNKQSVLVLMPNIRSQTQDSLYWGKIVDGIALALDQVGLGMVIVSEHRADNFI 122 +P+ S + +++ +++ +D+ ++ K++ GI G V+V+ + D F Sbjct: 66 NPSFLSKSHLKNICLIIE--ERNFKDTNFYTKVIWGIENEAKANGYD-VLVNFMKKDLFE 122 Query: 123 --NILNPSGLLGLVGVGQISTSLLLEVHRIGLPMVLIDHEDPLIPSDTVFANNIDSMTRL 180 + + + G++ VG I L E+ +P VL+DH + +D V NI R Sbjct: 123 IPSCIENRKVSGILVVGTIKDEFLAEILSYNIPTVLVDHASFMFSTDAVLTQNIPGSYRG 182 Query: 181 SNHLMGIGHTLLHFIGNIRYSRSFRDRWIGFRSALEENDMKTPAGDDEMLVLEGMEDGTF 240 + +L+ GH + F G +S SFR+RW+GF+ A+ D P +D V E +E Sbjct: 183 TKYLIEKGHKKIGFFGEKDFSLSFRERWLGFKEAMR--DAGLPVNED-YCVTEDVERHVL 239 Query: 241 RDDFRLWLAKRKKAKTIPSALVCANDSTALIVCEVLREEGVAIPADISVTGFDNIEDAMR 300 ++ + KK K P+A VC+NDS A+ + L G+ +P D+S+ GFD+I+ Sbjct: 240 SKNYEEVANRLKKLKEFPTAWVCSNDSNAITLYNALNIMGIKVPKDVSIVGFDDIDMCNI 299 Query: 301 GIPPLTTVHVPKEAMGRTAVEKLMNRIQNPSAPLEKILIAADIVHRDSV 349 PPLTT+H+ KE MG AV++L+ R+ NP + I + V R SV Sbjct: 300 VTPPLTTIHINKELMGIKAVKRLLWRMDNPKEFHDHIRMEVGFVERQSV 348 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,079,412,882 Number of extensions: 86926491 Number of successful extensions: 245066 Number of sequences better than 1.0e-05: 6093 Number of HSP's gapped: 238751 Number of HSP's successfully gapped: 6101 Length of query: 354 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 217 Effective length of database: 1,770,017,148 Effective search space: 384093721116 Effective search space used: 384093721116 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)