BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0012.p (623 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAQ8.1 B3KAQ8_9BACI Histidine kinase internal region 497 e-138 B1DGV8.1 B1DGV8_9BACL Integral membrane sensor signal transducti... 488 e-136 B1DAF1.1 B1DAF1_9BACL Integral membrane sensor signal transducti... 423 e-116 B1DBG2.1 B1DBG2_9BACL Histidine kinase internal region 244 2e-62 A9KIC4.1 A9KIC4_CLOPH Multi-sensor signal transduction histidine... 227 3e-57 >B3KAQ8.1 B3KAQ8_9BACI Histidine kinase internal region Length = 625 Score = 497 bits (1279), Expect = e-138, Method: Compositional matrix adjust. Identities = 258/600 (43%), Positives = 367/600 (61%), Gaps = 6/600 (1%) Query: 18 MERKLLLVFLIIITLPLSVISVISFKSYSESIQANTVAYSEKLIDQMMDSIDDYIEDMKR 77 ++RKL +VFL +I LP+ +I+ + + Y+ +I+ NTVAY +L +MM +DDY+ DM + Sbjct: 19 LDRKLFVVFLFLIILPIGLITYFAAERYTATIENNTVAYVSQLSSKMMGKLDDYVTDMMK 78 Query: 78 ISSMPAYVNDIKQNLIRSNRYYEQKQMMDSKGGSTPVAPGDFDLLLSIQRGIEGNISFIN 137 IS +P+Y+N+I+ L SNR+Y+ + P + + +L L I R +E +I F+N Sbjct: 79 ISIIPSYLNEIQSGLEMSNRHYQAEGQ-----PKEPASLNESELKLQITRKVERSIYFMN 133 Query: 138 NIKRGTNSVYIFDGYGNGYYSAKDGGVRLDLEQSYKFWSGQSRDSSGEALLFGTQAYTSN 197 NIK GT++VY+FD +GN YY K GG R +L+Q Y W + ++ G +L TQ + Sbjct: 134 NIKEGTSNVYLFDLFGNPYYVVKSGGNRSNLQQYYDNWRKLATEAGGRPVLVSTQEIAVS 193 Query: 198 LQSTRYAFTVVRKIVDGLWNPIGLIAVEANISNLENQVAELDTVTHGKSIIVDEGGKVIY 257 S +Y FTVVR I+D ++ IG I V+ANI +EN V +L+ THG + I+D+ G VIY Sbjct: 194 AGSKQYMFTVVRDIIDKSYHSIGTIVVDANIGVIENIVTDLEETTHGTTFILDDNGTVIY 253 Query: 258 DSGRKLLTTDISRTSLFRQAVGNAGSFYDTVSGKEQLNIYSSSAKTNWKVIISIPVDELT 317 DS +K L+ ++ + QA GN GSF V G+EQL +Y S +T W ++I++P +L Sbjct: 254 DSEKKYLSRSMTDNAALAQATGNEGSFRHKVDGEEQLVVYRKSEQTGWLILITVPERQLM 313 Query: 318 RGVKLTRNXXXXXXXXXXXXXXXXXXXXXXXXTKPLTQMIQLMKRVQNGDLDVTFRVRRR 377 + TR T+PL ++ LMK+VQ G LDV F VRRR Sbjct: 314 QDALKTRKITIAAAVGVTSFALIISLILIYALTRPLRSIVHLMKQVQTGKLDVVFPVRRR 373 Query: 378 DEIGLLGHQFNRMLARIRQLIQDIYRIEEQKKEAELQALQSQINPHFIYNTLESIRMTAE 437 DE L+G FNRM+ARI+QLI DIY IE++KKEAEL+ LQ QINPHFIYNTLE+IRMTA Sbjct: 374 DEASLIGVSFNRMMARIKQLIDDIYAIEKRKKEAELERLQHQINPHFIYNTLETIRMTAV 433 Query: 438 INDDVEAADMVSILGKLLRYSTSDLSGTTTMKQELLYVRNYVELLGCRYPGRFVLDIDVP 497 +++D E DMV +LG+ LRYS S +K+E +V+ Y+ELL RY RFVL + Sbjct: 434 LHNDQEVGDMVQLLGQQLRYSIHAGSEVVEVKREWEHVKAYIELLNYRYGERFVLTLPDD 493 Query: 498 AALDNYSMIKLVFQPIIENAAYHGLDDTKPRMHLSIACEVTEQKLLFHIRDDGCGMEQAT 557 D+ ++KL+FQPIIENA HG D+ K +HL+I + +F + DDG GM Sbjct: 494 PGADSIRVMKLLFQPIIENAVNHGFDENKSVLHLTITYAMDGMNHVFTVTDDGLGMSAEA 553 Query: 558 LDKLNDSLKHEMPPKKSINGGIGMKNVHQRIQLHYGAAYGIEVFSRPGEGTDVILSLPLQ 617 L +L L PP G+G++NVH+R+QL YG YG+ V S G GT V ++ P + Sbjct: 554 LARLRSMLDQAQPPGTD-GRGVGLRNVHERLQLRYGRPYGLTVDSEEGRGTAVRITFPAE 612 >B1DGV8.1 B1DGV8_9BACL Integral membrane sensor signal transduction histidine kinase Length = 622 Score = 488 bits (1256), Expect = e-136, Method: Compositional matrix adjust. Identities = 266/608 (43%), Positives = 385/608 (63%), Gaps = 4/608 (0%) Query: 10 WSYIGDMAMERKLLLVFLIIITLPLSVISVISFKSYSESIQANTVAYSEKLIDQMMDSID 69 W+ ME KLL+VF+++I +P+++++++S + Y+++I+ NTV+Y+ +L D+M+ +D Sbjct: 11 WTEWPSTRMEAKLLIVFVLLILVPIALLTLLSAQRYTQTIERNTVSYASELTDKMIGKLD 70 Query: 70 DYIEDMKRISSMPAYVNDIKQNLIRSNRYYEQKQMMDSKGGSTPVAPGDFDLLLSIQRGI 129 DY DMK+IS +P+Y+++I+ L +SN YY ++ +++KG T A D + L IQR I Sbjct: 71 DYTVDMKKISIIPSYLDEIQAGLKQSNDYYAKE--LEAKGTLTDTAAEDDTVKLKIQRKI 128 Query: 130 EGNISFINNIKRGTNSVYIFDGYGNGYYSAKDGGVRLDLEQSYKFWSGQSRDSSGEALLF 189 E +I F+NNIK GT+++Y+FD YGN YY K GGVR +L Y W + ++ G+ +L Sbjct: 129 ESSIYFMNNIKEGTSNIYLFDRYGNPYYVIKSGGVRSNLSDYYGNWQQIADEAHGKPVLV 188 Query: 190 GTQAYTSNLQSTRYAFTVVRKIVDGLWNPIGLIAVEANISNLENQVAELDTVTHGKSIIV 249 TQ T+ + +Y FTVVR+I+D +NPIGLIAV+ANIS + N V +LD THG ++I+ Sbjct: 189 STQEVTT-VNRKQYVFTVVREIIDKSYNPIGLIAVDANISVITNIVRDLDATTHGTTLIL 247 Query: 250 DEGGKVIYDSGRKLLTTDISRTSLFRQAVGNAGSFYDTVSGKEQLNIYSSSAKTNWKVII 309 DE VIYDS +K L ++S L +Q G GSF+ QL IY S + W ++I Sbjct: 248 DEQKNVIYDSEQKYLLQNLSDNELLKQVTGKEGSFHIGEKAHSQLVIYRKSEVSGWLMLI 307 Query: 310 SIPVDELTRGVKLTRNXXXXXXXXXXXXXXXXXXXXXXXXTKPLTQMIQLMKRVQNGDLD 369 SIP +L TRN TKPL +++LMK+VQ+G LD Sbjct: 308 SIPEKQLKSEALRTRNYTTAAAISTMCLALVISLITIFALTKPLRSLVKLMKQVQSGKLD 367 Query: 370 VTFRVRRRDEIGLLGHQFNRMLARIRQLIQDIYRIEEQKKEAELQALQSQINPHFIYNTL 429 V F ++RRDE+G++G+ FNRM+ARI LI DIYRIE++KK+ EL++LQ QINPHFIYNTL Sbjct: 368 VMFPIKRRDEVGMVGNAFNRMMARIVSLIDDIYRIEQRKKQIELESLQRQINPHFIYNTL 427 Query: 430 ESIRMTAEINDDVEAADMVSILGKLLRYSTSDLSGTTTMKQELLYVRNYVELLGCRYPGR 489 ESIRMTA INDD E MV +LG+ LRYS S T KQE ++R Y++LL RY R Sbjct: 428 ESIRMTAVINDDPEVGQMVQLLGQQLRYSIHAGSETVQAKQEWEHLRMYMQLLSYRYGTR 487 Query: 490 FVLDIDVPAALDNYSMIKLVFQPIIENAAYHGLDDTKPRMHLSIACEVTEQKLLFHIRDD 549 + L + A+ ++KL+FQPI+ENA H D+ + +SI F ++D+ Sbjct: 488 YSLKLPEDPAIGKIEVMKLLFQPIVENAINHAYDEQTGDLVISIGYRQAGGDQYFIVKDE 547 Query: 550 GCGMEQATLDKLNDSLKHEMPPKKSINGGIGMKNVHQRIQLHYGAAYGIEVFSRPGEGTD 609 G GM++ L +L L E P+ GIG++NV++R++L YG AYGI V S GEGT Sbjct: 548 GSGMDEKALQRLRSMLDAEQMPEWE-GRGIGLRNVNERLKLRYGPAYGITVDSVSGEGTT 606 Query: 610 VILSLPLQ 617 V + LP+Q Sbjct: 607 VSIKLPIQ 614 >B1DAF1.1 B1DAF1_9BACL Integral membrane sensor signal transduction histidine kinase Length = 596 Score = 423 bits (1088), Expect = e-116, Method: Compositional matrix adjust. Identities = 238/616 (38%), Positives = 365/616 (59%), Gaps = 26/616 (4%) Query: 1 MKAKRSHSWWSYIGDMAMERKLLLVFLIIITLPLSVISVISFKSYSESIQANTVAYSEKL 60 M R ++ M M++KL++VF+++I +P+S IS+++Y +SI NT Y +L Sbjct: 1 MNIGRGFFRFNGFSSMTMQKKLIIVFVVLIIIPISATGFISYQNYIQSINKNTKNYVTEL 60 Query: 61 IDQMMDSIDDYIEDMKRISSMPAYVNDIKQNLIRSNRYYEQKQMMDSKGGSTPVAPGDFD 120 I M ++D ++EDM +S +P Y +++Q Y ++M Sbjct: 61 ISNMQANLDSHLEDMMTVSKIPLYSVEMQQ-------YMSHQEMT--------------- 98 Query: 121 LLLSIQRGIEGNISFINNIKRGTNSVYIFDGYGNGYYSAKDGGVRLDLEQSYKFWSGQSR 180 L QR ++ I ++++++ S Y++D YGN +Y K +R D+ Y+ W + Sbjct: 99 --LEKQRQMDFYIGLLDSMRKNLYSAYLYDNYGNVFYRIKTDAIREDMMSHYEEWKRLAI 156 Query: 181 DSSGEALLFGTQAYTSNLQSTRYAFTVVRKIVD-GLWNPIGLIAVEANISNLENQVAELD 239 + +G ++ TQ + +Y FTV+R I D G IG +AV+A+I +E+ V EL+ Sbjct: 157 EGNGNPVIIPTQRVMRANGTPQYLFTVLRSIRDIGTLETIGYVAVDADIHVIEDVVQELN 216 Query: 240 TVTHGKSIIVDEGGKVIYDSGRKLLTTDISRTSLFRQAVGNAGSFYDTVSGKEQLNIYSS 299 TVT+GK++I+D V YDS L T+I+ + ++AVG+ GSF V G + YS Sbjct: 217 TVTNGKTLIIDGQNNVAYDSDHVLTATNITGNEVIQRAVGDQGSFNVVVDGSSYICSYSV 276 Query: 300 SAKTNWKVIISIPVDELTRGVKLTRNXXXXXXXXXXXXXXXXXXXXXXXXTKPLTQMIQL 359 S T WK+ +PVD L +LTRN T+ L QM L Sbjct: 277 SKDTGWKIFAFVPVDYLHHDAQLTRNVTLTTTIGIIGFALLVSVILAFMMTRSLRQMTDL 336 Query: 360 MKRVQNGDLDVTFRVRRRDEIGLLGHQFNRMLARIRQLIQDIYRIEEQKKEAELQALQSQ 419 M+ V+ G+LDV+F V+++DE+G+LG +FNRML R+++LI +IY I+ +KKEA+L+ALQSQ Sbjct: 337 MRTVRGGNLDVSFDVKQKDEVGILGTEFNRMLRRMKELISEIYVIQSRKKEADLEALQSQ 396 Query: 420 INPHFIYNTLESIRMTAEINDDVEAADMVSILGKLLRYSTSDLSGTTTMKQELLYVRNYV 479 INPHFIYNTLE+IRMTAEINDD E A+M LGKLLRYS + T +++EL ++ Y+ Sbjct: 397 INPHFIYNTLETIRMTAEINDDEEVAEMTFTLGKLLRYSINRGRETVELREELQHLERYL 456 Query: 480 ELLGCRYPGRFVLDIDVPAALDNYSMIKLVFQPIIENAAYHGLDDTKPRMHLSIACEVTE 539 +LL R+ RFV+ D+P L N+ ++KL FQPI+ENA HG++ + + ++ E+ E Sbjct: 457 QLLRYRFSNRFVVAFDIPQELMNFPVMKLTFQPIVENAILHGMEGKPNEVTIRLSAELKE 516 Query: 540 QKLLFHIRDDGCGMEQATLDKLNDSLKHEMPPKKSINGGIGMKNVHQRIQLHYGAAYGIE 599 Q L+F I+D+G GM+ TLD L ++ S G+G++NV++RI+LHYG YG+E Sbjct: 517 QLLIFSIKDEGAGMDGMTLDLLRQAMNGTRESASS-KTGVGLRNVNERIKLHYGETYGLE 575 Query: 600 VFSRPGEGTDVILSLP 615 + S+ GEGT V L LP Sbjct: 576 IESKWGEGTTVNLKLP 591 >B1DBG2.1 B1DBG2_9BACL Histidine kinase internal region Length = 595 Score = 244 bits (622), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 173/628 (27%), Positives = 308/628 (49%), Gaps = 58/628 (9%) Query: 4 KRSHSWWSYIGDMAMERKLLLVFLIIITLPLSVISVISFKSYSESIQANTVAYSEKLIDQ 63 +R W+ ++A+ RKL+LV +++I +PL VI SF ++ ++ Y +++ Q Sbjct: 5 RRLQQWFK---NLALARKLILVNVLLIVVPLGVIGAWSFSIFASKMEEQVGKYQLQMLKQ 61 Query: 64 MMDSIDDYIEDMKRISSMPAYVNDIKQNLIRSNRYYEQKQMMDSKGGSTPVAPGDFDLLL 123 + +ID Y+ ++ R+S MP Y+ ++++D +T PG+ L Sbjct: 62 VTLNIDTYMNELNRLSLMP----------------YQYQEILDFL--ATSRKPGE---AL 100 Query: 124 SIQRGIEGNISFINNI----KRGTNSVYIFDGYGNGYYSAKDGGVRLDLEQSYKF----- 174 S+Q I+ +F++ + + + +F +G Y + +Y+ Sbjct: 101 SLQE-IDDLNNFVSKVFLNGRVDIMGISLFGEHGASYVVLPES----QYMTTYQLGGNTE 155 Query: 175 WSGQSRDSSGEALLFGTQAYTSNLQSTRYAFTVVRKI--VDGLWNPIGLIAVEANISNLE 232 W +++ G+ T S + F++ R++ D + +G I ++ + + + Sbjct: 156 WLEKAKGRFGQPTFITTHEVKSTSGTAYQVFSIARELRSFDS-GDTLGYIVIDIDPAEVR 214 Query: 233 NQVAELDTVTHGKSIIVDEGGKVIYDSGRKLLTTDISRTSLFR--QAVGNAGSFYDTVSG 290 ++++ + + D G ++ RK +T+++ + + + G + + G Sbjct: 215 KLLSQVSLGSKESLYMTDSAGDLVL---RK------EKTAIYPVIEGLSDEGVSHHKIDG 265 Query: 291 KEQLNIYSSSAKTNWKVIISIPVDELTRGVKLTRNXXXXXXXXXXXXXXXXXXXXXXXXT 350 K L + SS T W + +PV L + + RN T Sbjct: 266 KSLLVAHVSSEVTGWTTVGVVPVSVLMKDSLVVRNSIILIGGICVGLAVLLSVFIAFRIT 325 Query: 351 KPLTQMIQLMKRVQNGDLDVTFRVRRRDEIGLLGHQFNRMLARIRQLIQDIYRIEEQKKE 410 PL + LM++V+ GDL V+F VR+ DE+G LG+ FN M++ + +L +Y E ++K+ Sbjct: 326 SPLRMLRSLMRKVERGDLKVSFPVRQWDEVGQLGNAFNMMVSHLSELGYRLYETEIREKD 385 Query: 411 AELQALQSQINPHFIYNTLESIRMTAEINDDVEAADMVSILGKLLRYSTSDLSGTTTMKQ 470 AE+ ALQS+INPHF+YNTL SI M AE+ + E M + L +LLRYS S T T++ Sbjct: 386 AEIAALQSKINPHFLYNTLGSISMYAEVEGNREVVTMTNNLSRLLRYSLSGRKETVTLRD 445 Query: 471 ELLYVRNYVELLGCRYPGRFVLDIDVPAALDNYSMIKLVFQPIIENAAYHGLDDTKPRMH 530 EL +V+ Y+ + RY R ++V +L +I L+ QPI+ENA HGLD Sbjct: 446 ELDHVKGYMTIQKMRYDDRIDFRLEVDESLYGCPVIPLMIQPIVENAINHGLDKGIGEGR 505 Query: 531 LSIACEVTEQKLLFHIRDDGCGMEQATLDKLNDSLKHEMPPKKSING--GIGMKNVHQRI 588 + ++C + L+ I DDG GM L +++ M + G G G+ NVH+RI Sbjct: 506 IQLSCYQEQGMLMIVIEDDGIGMTSEQL----SAMRKRMDKIHDLGGTSGNGLMNVHRRI 561 Query: 589 QLHYGAAYGIEVFSRPGEGTDVILSLPL 616 +LHYG YG+++ S P +G VIL++P+ Sbjct: 562 RLHYGEQYGLQLESMPFQGLKVILTMPI 589 >A9KIC4.1 A9KIC4_CLOPH Multi-sensor signal transduction histidine kinase Length = 585 Score = 227 bits (578), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 175/602 (29%), Positives = 299/602 (49%), Gaps = 39/602 (6%) Query: 21 KLLLVFLIIITLPLSVISVISFKSYSESIQANTVAYSEKLIDQMMDSIDDYIEDMKRISS 80 K+L+ FLI+I + + + IS S+ N++ Y+ +LI+QM +D YI M+ IS Sbjct: 11 KILVSFLILIIPMIMIAARISMTYTENSVVTNSMEYTSELINQMNSDVDSYIGYMENISQ 70 Query: 81 MPAYVNDIKQNLIRSNRYYEQKQMMDSKGGSTPVAPGDFDLLLSIQRGIEGNISFINNIK 140 + +D+ L N YE+ +DS+ + F +L ++ I N++ I Sbjct: 71 LALLDSDVHTFL---NSDYEE---LDSRQFAKERVMNQFGTILDLRSDI-TNLAII---- 119 Query: 141 RGTNSVYIFDGYGNGYYSAKDGGVRLDLEQSYKFWSGQSRDSSGEALLFGTQAYTSNLQS 200 GTN YI + + LD++Q K W ++ +G ++ + ++ N+ + Sbjct: 120 -GTNGRYILNR------GEDEINPYLDVKQ--KEWYKKA--IAGNGKIYISSSHVQNIVN 168 Query: 201 TRYAFTVV--RKIVDGLWNP-IGLIAVEANISNLENQVAELDTVTHGKSIIVDEGGKVIY 257 +Y + V R I D + IG + V+ N + + N +++ G ++DE G +IY Sbjct: 169 GKYQWVVSLSRAIKDKESDEMIGALLVDLNYNVINNLCKKINLGYKGYIFLLDEEGNIIY 228 Query: 258 DSGRKLLTTDI--SRTSLFRQAVGNAGSFYDTVSGKEQLNIYSSSAKTNWKVIISIPVDE 315 ++L+ + +T ++ S++ T +L + S S KT W + V E Sbjct: 229 HPKQQLIYNGLINEKTLEVKEC---KESYFLTEGTDRKLYVISRSEKTGWTAVGVANVSE 285 Query: 316 LTRGVKLTRNXXXXXXXXXXXXXXXXXXXXXXXXTKPLTQMIQLMKRVQNGDLDVTFRVR 375 L + T+ TKP+ ++ MK V+ G+ Sbjct: 286 LFLQREQTQRLYLISTVAILLIAMLLSILISRAITKPIKKLRDSMKEVEKGNFHEKVETS 345 Query: 376 RRDEIGLLGHQFNRMLARIRQLIQDIYRIEEQKKEAELQALQSQINPHFIYNTLESIRMT 435 DEI LG +N M+ + +L++ + +EQK+++EL+ALQ+QINPHF+YNTL+SI Sbjct: 346 GSDEIANLGKSYNIMIQTVDELMEARVKDQEQKRKSELRALQAQINPHFLYNTLDSIVWM 405 Query: 436 AEINDDVEAADMVSILGKLLRYSTSDLSGTTTMKQELLYVRNYVELLGCRYPGRFVLDID 495 AE + E M + L KL R S S+ + T++QE+ YVRNY+ + RY + DI Sbjct: 406 AEEGKNQEVVTMTASLAKLFRQSISNEAEEFTLRQEVDYVRNYLVIQKMRYKEQLNYDIQ 465 Query: 496 VPAALDNYSMIKLVFQPIIENAAYHGLDDTKPRMHLSIACEVTEQKLLFHIRDDGCGMEQ 555 VP ++ NY +IKL+ QP++ENA YHG+ + + TE ++ + DDG GM++ Sbjct: 466 VPESILNYYVIKLILQPLVENALYHGVKYKDGVGTIFVDGYETESTVVLQVIDDGIGMDE 525 Query: 556 ATLDKLNDSLKHEMPPKKSIN--GGIGMKNVHQRIQLHYGAAYGIEVFSRPGEGTDVILS 613 + L H +KS + + ++N+H RIQLHYG +G+ S+ G GT V+++ Sbjct: 526 -------EELSHLFEKRKSTHKTSRVAIRNIHNRIQLHYGKEFGLSFESKKGYGTKVMIT 578 Query: 614 LP 615 LP Sbjct: 579 LP 580 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,388,206,194 Number of extensions: 138187154 Number of successful extensions: 408436 Number of sequences better than 1.0e-05: 2345 Number of HSP's gapped: 405481 Number of HSP's successfully gapped: 2346 Length of query: 623 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 481 Effective length of database: 1,722,912,073 Effective search space: 828720707113 Effective search space used: 828720707113 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)