BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0010.p (441 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFL8.1 B3KFL8_9BACI Peptidase U32 704 0.0 B7GIT9.1 B7GIT9_ANOFW Collagenase family protease 523 e-146 O32034.1 YRRO_BACSU Uncharacterized protease yrrO EC=3.4.-.- 514 e-144 A4IR73.1 A4IR73_GEOTN Peptidase, U32 family 514 e-144 A7Z728.1 A7Z728_BACA2 YrrO 511 e-143 >B3KFL8.1 B3KFL8_9BACI Peptidase U32 Length = 447 Score = 704 bits (1818), Expect = 0.0, Method: Compositional matrix adjust. Identities = 342/446 (76%), Positives = 367/446 (82%), Gaps = 5/446 (1%) Query: 1 MGTMTKPQFKGKRYRLDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSF 60 M M +P++KGKRYRLDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRS ADNFSF Sbjct: 1 MEAMAQPKYKGKRYRLDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSNADNFSF 60 Query: 61 EEMREGVEFAKKYGAKVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXR 120 EEM+EGVEFAKKYGAKVFVATNIYAHNEDI GIE YLR+L E + Sbjct: 61 EEMKEGVEFAKKYGAKVFVATNIYAHNEDIEGIESYLRSLEEAGIAAIIAADPAIIEVAK 120 Query: 121 RLVPGLEVHLSTQQSTLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIH 180 R P LEVHLSTQQSTLNWQAV FWK+EGLPRVVLGRE SLEEIA IK+HVDIEIE+FIH Sbjct: 121 RAAPKLEVHLSTQQSTLNWQAVKFWKDEGLPRVVLGREASLEEIAAIKEHVDIEIEAFIH 180 Query: 181 GAMCSSYSGRCVLSNHFTDRDSNRGGCCQSCRWKYDLFEDARPE-GTWVSEEDXXXXXXX 239 GAMCSS+SGRCVLSNHFTDRDSNRGGCCQSCRWKYDLFED R E G WVSEE Sbjct: 181 GAMCSSFSGRCVLSNHFTDRDSNRGGCCQSCRWKYDLFEDGREEQGHWVSEEQQLMEQPA 240 Query: 240 XXXXXXXXXXXX----XXEDNPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVA 295 EDN F+MGSKDLCMLE IP+LIE GIDSFKIEGRMKSIHYVA Sbjct: 241 PAPSPFKPGVTQIPLFQPEDNQFTMGSKDLCMLEHIPDLIEVGIDSFKIEGRMKSIHYVA 300 Query: 296 TVVNAYRKAIDAYMADPEGYVLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIYEPEEKA 355 TVVN YR+AID+YMADP+ YVLKPEW+EE+ KAANRPLNTGFFYDTPDHEDHIYEPEEKA Sbjct: 301 TVVNVYRQAIDSYMADPDHYVLKPEWIEEINKAANRPLNTGFFYDTPDHEDHIYEPEEKA 360 Query: 356 APYDFAGLVLEYDAAGGTALIQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDA 415 PYDFAGLV+EYD GTA+IQQRNHFKPG ++EFFGP+ TFFKQTVG I DE G LDA Sbjct: 361 VPYDFAGLVMEYDEHSGTAVIQQRNHFKPGDQIEFFGPEGTFFKQTVGPIQDENGSELDA 420 Query: 416 ARHPLQRVRMKVDQPVAYFDMMRKKK 441 ARHPLQ +RMKVDQPV +FDMMRK+K Sbjct: 421 ARHPLQLIRMKVDQPVRHFDMMRKRK 446 >B7GIT9.1 B7GIT9_ANOFW Collagenase family protease Length = 417 Score = 523 bits (1347), Expect = e-146, Method: Compositional matrix adjust. Identities = 257/431 (59%), Positives = 304/431 (70%), Gaps = 22/431 (5%) Query: 9 FKGKRYRLDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSFEEMREGVE 68 GKR + KPELLAPAGNLEKLK AVHYGADAV+IGGQ+YGLRS ADNF+ EEM EGV+ Sbjct: 7 INGKRVIVKKPELLAPAGNLEKLKVAVHYGADAVFIGGQEYGLRSNADNFTLEEMAEGVQ 66 Query: 69 FAKKYGAKVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXRRLVPGLEV 128 FAKKYGAK++V TNIYAHNE+I G+E+YLR L RR+ P LEV Sbjct: 67 FAKKYGAKIYVTTNIYAHNENIPGLEDYLRGLERVGVHGIIVADPLIIETARRVAPKLEV 126 Query: 129 HLSTQQSTLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIHGAMCSSYS 188 HLSTQQS NW+AV FWKEEGL RVVL RETS EEI EIK+ VDIEIE+FIHGAMC +YS Sbjct: 127 HLSTQQSLTNWKAVQFWKEEGLERVVLARETSAEEIREIKEKVDIEIETFIHGAMCIAYS 186 Query: 189 GRCVLSNHFTDRDSNRGGCCQSCRWKYDLFEDARPEGTWVSEEDXXXXXXXXXXXXXXXX 248 GRCVLSNH T RDSNRGGCCQSCRW YDL++ + + ++ Sbjct: 187 GRCVLSNHMTARDSNRGGCCQSCRWDYDLYKLEGDQAVALFDK----------------- 229 Query: 249 XXXXXEDNPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVATVVNAYRKAIDAY 308 ED+PF+M KDL +++SIP++IE GIDS KIEGRMKSIHYVATVV+ YRK IDAY Sbjct: 230 -----EDDPFAMSPKDLNLIQSIPKMIELGIDSLKIEGRMKSIHYVATVVSVYRKVIDAY 284 Query: 309 MADPEGYVLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIYEPEEKAAPYDFAGLVLEYD 368 ADP+ +V++ EWL+EL K ANR FF P +++ ++ K YDF GLVL+YD Sbjct: 285 CADPDNFVIRKEWLDELDKCANRDTAPAFFEGVPGYKEQMFGVHSKKTTYDFVGLVLDYD 344 Query: 369 AAGGTALIQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDAARHPLQRVRMKVD 428 G +QQRN FKPG EVEFFGP+ F Q + IWDEEG LDAARHPLQ VR KV+ Sbjct: 345 KETGIVTLQQRNFFKPGDEVEFFGPEIENFTQVIENIWDEEGNELDAARHPLQIVRFKVE 404 Query: 429 QPVAYFDMMRK 439 + V ++MMRK Sbjct: 405 REVFPYNMMRK 415 >O32034.1 YRRO_BACSU Uncharacterized protease yrrO EC=3.4.-.- Length = 422 Score = 514 bits (1325), Expect = e-144, Method: Compositional matrix adjust. Identities = 255/431 (59%), Positives = 299/431 (69%), Gaps = 22/431 (5%) Query: 11 GKRYRLDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSFEEMREGVEFA 70 GKR KPELLAPAGNLEKLK AVHYGADAV+IGGQ+YGLRS ADNF+ EE+ EGVEFA Sbjct: 14 GKRVITKKPELLAPAGNLEKLKIAVHYGADAVFIGGQEYGLRSNADNFTIEEIAEGVEFA 73 Query: 71 KKYGAKVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXRRLVPGLEVHL 130 KKYGAK++V TNI+AHNE++ G+E+YL+ L + RR+ P +EVHL Sbjct: 74 KKYGAKIYVTTNIFAHNENMDGLEDYLKALGDANVAGIIVADPLIIETCRRVAPNVEVHL 133 Query: 131 STQQSTLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIHGAMCSSYSGR 190 STQQS NW+AV FWKEEGL RVVL RETS EI E+K+ VDIEIESFIHGAMC +YSGR Sbjct: 134 STQQSLSNWKAVQFWKEEGLDRVVLARETSALEIREMKEKVDIEIESFIHGAMCIAYSGR 193 Query: 191 CVLSNHFTDRDSNRGGCCQSCRWKYDLFEDARPEGTWVSEEDXXXXXXXXXXXXXXXXXX 250 CVLSNH T RDSNRGGCCQSCRW YDL++ + E Sbjct: 194 CVLSNHMTARDSNRGGCCQSCRWDYDLYQTDGANAVALYGE------------------- 234 Query: 251 XXXEDNPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVATVVNAYRKAIDAYMA 310 ED PF+M KDL ++ESIP++IE GIDS KIEGRMKSIHYVATVV+ YRK IDAY A Sbjct: 235 ---EDAPFAMSPKDLKLIESIPKMIEMGIDSLKIEGRMKSIHYVATVVSVYRKVIDAYCA 291 Query: 311 DPEGYVLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIYEPEEKAAPYDFAGLVLEYDAA 370 DPE +V++ EWLEEL K ANR T FF TP +E+ ++ K YDF GLVL YD Sbjct: 292 DPENFVIQKEWLEELDKCANRDTATAFFEGTPGYEEQMFGEHAKKTTYDFVGLVLNYDED 351 Query: 371 GGTALIQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDAARHPLQRVRMKVDQP 430 +QQRN FK G EVEFFGP+ F T+ IWDE+G LDAARHPLQ V+ K+D+ Sbjct: 352 TQMVTLQQRNFFKKGDEVEFFGPEIENFTHTIETIWDEDGNELDAARHPLQIVKFKLDKK 411 Query: 431 VAYFDMMRKKK 441 + +MMRK K Sbjct: 412 IYPSNMMRKGK 422 >A4IR73.1 A4IR73_GEOTN Peptidase, U32 family Length = 423 Score = 514 bits (1324), Expect = e-144, Method: Compositional matrix adjust. Identities = 252/432 (58%), Positives = 305/432 (70%), Gaps = 25/432 (5%) Query: 11 GKRYRLDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSFEEMREGVEFA 70 GKR + KPELLAPAGNLEKLK AVHYGADAV+IGGQ+Y LR+ ADNF+ EE+REGVEFA Sbjct: 14 GKRVIVKKPELLAPAGNLEKLKIAVHYGADAVFIGGQEYSLRANADNFTIEEIREGVEFA 73 Query: 71 KKYGAKVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXRRLVPGLEVHL 130 +YGAKV+V NIYAHNE+I G+++YLR L + RR+ P LEVHL Sbjct: 74 NQYGAKVYVTANIYAHNENIPGLDDYLRALEDAGVHGIIVADPLIIETARRVAPKLEVHL 133 Query: 131 STQQSTLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIHGAMCSSYSGR 190 STQQS NW+AV FWKEEGL RVVL RE S EEI +IK+ VDIEIE+FIHGAMCS+YSGR Sbjct: 134 STQQSLANWKAVQFWKEEGLERVVLAREVSAEEIRQIKEKVDIEIEAFIHGAMCSAYSGR 193 Query: 191 CVLSNHFTDRDSNRGGCCQSCRWKYDLFE--DARPEGTWVSEEDXXXXXXXXXXXXXXXX 248 CVLSNH T RDSNRGGCCQSCRW YDL++ D E SE D Sbjct: 194 CVLSNHMTARDSNRGGCCQSCRWDYDLYQLSDDGREIPLFSENDA--------------- 238 Query: 249 XXXXXEDNPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVATVVNAYRKAIDAY 308 PF+M +KDL ++ +IP +IE G+DS KIEGRMKSIHYVATVV+ YRK IDAY Sbjct: 239 --------PFAMSAKDLNLIRAIPAMIELGVDSLKIEGRMKSIHYVATVVSVYRKVIDAY 290 Query: 309 MADPEGYVLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIYEPEEKAAPYDFAGLVLEYD 368 ADP+ + ++ EW+ EL K ANR + FF PD+ +H+Y + ++FAGLVL+YD Sbjct: 291 CADPDHFTIREEWVRELDKCANRETASSFFEGFPDYTNHMYGTHSRKTTHEFAGLVLDYD 350 Query: 369 AAGGTALIQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDAARHPLQRVRMKVD 428 G A IQQRNHFKPG EVEFFGP+ F Q + +IWDE+G LDAARHPLQ V++ V Sbjct: 351 RETGIATIQQRNHFKPGDEVEFFGPEIENFTQVIEKIWDEDGNELDAARHPLQIVKVPVK 410 Query: 429 QPVAYFDMMRKK 440 +P+ ++MMRK+ Sbjct: 411 RPLFPYNMMRKE 422 >A7Z728.1 A7Z728_BACA2 YrrO Length = 422 Score = 511 bits (1316), Expect = e-143, Method: Compositional matrix adjust. Identities = 254/431 (58%), Positives = 300/431 (69%), Gaps = 22/431 (5%) Query: 11 GKRYRLDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSFEEMREGVEFA 70 GKR KPELLAPAGNLEKLK AVHYGADAV+IGGQ+YGLRS ADNFS EE+ EGVEFA Sbjct: 14 GKRVITKKPELLAPAGNLEKLKIAVHYGADAVFIGGQEYGLRSNADNFSIEEIAEGVEFA 73 Query: 71 KKYGAKVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXRRLVPGLEVHL 130 KKYGAK++V TNI+AHNE++ G+EEYL+ L + RR+ P +E+HL Sbjct: 74 KKYGAKIYVTTNIFAHNENMDGLEEYLKALGDAKVAGIIVADPLIIETCRRVAPDVEIHL 133 Query: 131 STQQSTLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIHGAMCSSYSGR 190 STQQS NW+AV FWKEEGL RVVL RETS EI E+K+ VDIEIE+FIHGAMC +YSGR Sbjct: 134 STQQSLSNWKAVQFWKEEGLDRVVLARETSGLEIKEMKEKVDIEIETFIHGAMCIAYSGR 193 Query: 191 CVLSNHFTDRDSNRGGCCQSCRWKYDLFEDARPEGTWVSEEDXXXXXXXXXXXXXXXXXX 250 CVLSNH T RDSNRGGCCQSCRW YDL++ + +E Sbjct: 194 CVLSNHMTARDSNRGGCCQSCRWDYDLYQTDGANAVALYDE------------------- 234 Query: 251 XXXEDNPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVATVVNAYRKAIDAYMA 310 ED PF+M KDL ++ESIP++IE GIDS KIEGRMKSIHYVATVV+ YRK IDAY A Sbjct: 235 ---EDAPFAMSPKDLKLIESIPQMIEMGIDSLKIEGRMKSIHYVATVVSVYRKVIDAYCA 291 Query: 311 DPEGYVLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIYEPEEKAAPYDFAGLVLEYDAA 370 DPE +V++ EWL+EL K ANR FF TP +E+ ++ K +DFAGLVL Y+ Sbjct: 292 DPENFVIQKEWLDELDKCANRDTAPAFFEGTPGYEEQMFGEHGKKTTFDFAGLVLAYNEE 351 Query: 371 GGTALIQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDAARHPLQRVRMKVDQP 430 +QQRN FK G EVEFFGP+ F T+G IWDE+G LDAARHPLQ V KVD+ Sbjct: 352 TQMVTLQQRNFFKQGDEVEFFGPEIDNFTFTIGTIWDEDGNELDAARHPLQIVTFKVDKK 411 Query: 431 VAYFDMMRKKK 441 + +MMRK K Sbjct: 412 IYPSNMMRKGK 422 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,628,059,787 Number of extensions: 109825185 Number of successful extensions: 242607 Number of sequences better than 1.0e-05: 1843 Number of HSP's gapped: 238540 Number of HSP's successfully gapped: 2821 Length of query: 441 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 302 Effective length of database: 1,751,175,118 Effective search space: 528854885636 Effective search space used: 528854885636 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)