BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0009.p (310 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFL7.1 B3KFL7_9BACI Peptidase U32 454 e-126 Q65GT1.1 Q65GT1_BACLD YrrN 300 1e-79 Q9KDE0.1 Q9KDE0_BACHD Protease 296 1e-78 C2YWW7.1 C2YWW7_BACCE Peptidase, U32 296 1e-78 O32035.1 YRRN_BACSU Uncharacterized protease yrrN EC=3.4.-.- 295 3e-78 >B3KFL7.1 B3KFL7_9BACI Peptidase U32 Length = 310 Score = 454 bits (1168), Expect = e-126, Method: Compositional matrix adjust. Identities = 213/287 (74%), Positives = 242/287 (84%) Query: 24 GADALLIGDDRFGMRLAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYVK 83 GADALL+G+DRFGMRL G +L++ A+ V+LAH QG KVY SL LM N LL ELPAYVK Sbjct: 24 GADALLVGEDRFGMRLPGSLTLDEIAQVVQLAHGQGSKVYVSLNNLMTNDLLPELPAYVK 83 Query: 84 AIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLARE 143 A+G++GVD VEF DP+VL VK AP +KLHWNAEMT+TNY+TANYWG KGASRV+LARE Sbjct: 84 AVGDIGVDAVEFNDPSVLTTVKDLAPQVKLHWNAEMTATNYSTANYWGSKGASRVILARE 143 Query: 144 LNMDEMTEMVPLLEVEAQVQVHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGKERGLFLI 203 LNMDE+T+M P L++EAQVQVHGMTNIYHSKR+LV SYM QGRP + GSLGKERGLFLI Sbjct: 144 LNMDEVTDMKPQLQLEAQVQVHGMTNIYHSKRRLVNSYMQQQGRPVNDGSLGKERGLFLI 203 Query: 204 EAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVAYNAAVVK 263 EAER +EK+PIYED NGTHIMSS+DICILEDLH L+ AGV S K+E LLKP YN V++ Sbjct: 204 EAERQDEKYPIYEDINGTHIMSSEDICILEDLHLLMAAGVDSFKVETLLKPRHYNEVVIR 263 Query: 264 AYRHAIDLYAADPEGYAFQESWMDGIRALQDPERELSFGFFYKEQVY 310 YR AIDLYAAD YAFQE WMDGIRA+QDPERELSFGFFYKEQVY Sbjct: 264 TYRKAIDLYAADASAYAFQEEWMDGIRAIQDPERELSFGFFYKEQVY 310 >Q65GT1.1 Q65GT1_BACLD YrrN Length = 309 Score = 300 bits (768), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 146/288 (50%), Positives = 194/288 (67%), Gaps = 2/288 (0%) Query: 24 GADALLIGDDRFGMRLAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYVK 83 GADA +IG+ R+G+RLAG FS E+ EA+ AHE+G KVY ++ + N + EL Y+ Sbjct: 23 GADAFIIGEQRYGLRLAGEFSREEVKEAIIQAHEKGAKVYVAVNAIFHNDRVPELNDYLS 82 Query: 84 AIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLARE 143 + E G D V FGDPAVL A + AP +KLHWN E T+TN+ + NYWGRKGA R VLARE Sbjct: 83 FLDEAGADAVVFGDPAVLMAARESAPNMKLHWNTETTATNHYSCNYWGRKGAKRAVLARE 142 Query: 144 LNMDEMTEMVPLLEVEAQVQVHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGKERGLFLI 203 LNMD + E+ EVE ++QVHGMT ++ SKR L+ +Y +QG+ D KE+G++L Sbjct: 143 LNMDSIVEIKEKAEVEIEIQVHGMTCMFQSKRSLIGNYFEYQGKVMDIEGKKKEKGMYLH 202 Query: 204 EAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVAYNAAVVK 263 + ER N K+PI+EDENGTHIMS +D+CI+++L L+ AG+ S KI+G+LK V Y V Sbjct: 203 DKERGN-KYPIFEDENGTHIMSPNDVCIIDELEDLIDAGIDSFKIDGILKSVEYMTEVTA 261 Query: 264 AYRHAIDLYAADPEGYA-FQESWMDGIRALQDPERELSFGFFYKEQVY 310 YR AIDL AD E Y +E W++ I LQ R + GFF+KE VY Sbjct: 262 MYREAIDLCIADREAYEDKKEQWIERIEQLQPANRSIDTGFFFKETVY 309 >Q9KDE0.1 Q9KDE0_BACHD Protease Length = 309 Score = 296 bits (759), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 144/288 (50%), Positives = 197/288 (68%), Gaps = 2/288 (0%) Query: 24 GADALLIGDDRFGMRLAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYVK 83 GADA+LIG+ R+G+RLAG F+ +D +A +A E G KVY ++ + N +D L YV+ Sbjct: 23 GADAILIGEQRYGLRLAGEFTKQDVQKATVIAQELGAKVYVAMNAIFHNEKVDMLAEYVR 82 Query: 84 AIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLARE 143 + + G+DG+ FGDPAVL V+ AP +KLHWN E T+TN+ TANYWGRKGA R VLARE Sbjct: 83 FLRDTGIDGIVFGDPAVLMTVREVAPNMKLHWNTETTATNWYTANYWGRKGAKRAVLARE 142 Query: 144 LNMDEMTEMVPLLEVEAQVQVHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGKERGLFLI 203 ++MD + E+ EVE +VQVHGMT ++ SKRKLV +YM QG+ + + K+R +FL Sbjct: 143 ISMDAIVEIKEHAEVEIEVQVHGMTAMFQSKRKLVGNYMEFQGKNLEVENRDKKRNMFLY 202 Query: 204 EAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVAYNAAVVK 263 + ER K+PI+EDENGTHIMS +DICI+++L ++ AG+ S KI+G+LK Y V Sbjct: 203 DPER-EAKYPIFEDENGTHIMSPNDICIIDELEDMIDAGIDSFKIDGILKTTEYMEKVTS 261 Query: 264 AYRHAIDLYAADPEGY-AFQESWMDGIRALQDPERELSFGFFYKEQVY 310 YR AIDL A D E Y +E +++ I A+Q R + GFF+KE VY Sbjct: 262 LYRQAIDLCADDREAYEEKKEDFIEEIEAIQPVNRSIDTGFFFKETVY 309 >C2YWW7.1 C2YWW7_BACCE Peptidase, U32 Length = 309 Score = 296 bits (759), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 147/288 (51%), Positives = 194/288 (67%), Gaps = 2/288 (0%) Query: 24 GADALLIGDDRFGMRLAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYVK 83 GADA++IG+ +FG+RLAG FS ED +AVK+AHE G KVY ++ + N +DEL YV Sbjct: 23 GADAVMIGEQKFGLRLAGEFSREDVKQAVKIAHENGVKVYVAMNAMFHNDKVDELTDYVA 82 Query: 84 AIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLARE 143 + E+ VD + FGDPAVL AV+ AP ++LHWN E T+TN+ T NYWG++GA R VLARE Sbjct: 83 FLNEVSVDAIVFGDPAVLMAVREVAPNMQLHWNTETTATNWFTCNYWGQRGAKRAVLARE 142 Query: 144 LNMDEMTEMVPLLEVEAQVQVHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGKERGLFLI 203 L++DE+ E+ EVE +VQ+HGMT ++ SKR LV +Y +QGR D E +FL Sbjct: 143 LSLDEIVELKENAEVELEVQIHGMTCMFQSKRSLVGNYFEYQGRNLDIEKKKYEENMFLY 202 Query: 204 EAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVAYNAAVVK 263 + ER N K+PIYEDENGTHIMS +DIC +++L L+ AGV SLKI+G+LK AY V K Sbjct: 203 DPER-NNKYPIYEDENGTHIMSPNDICFIDELEELIDAGVDSLKIDGVLKSSAYIIEVTK 261 Query: 264 AYRHAIDLYAADPEG-YAFQESWMDGIRALQDPERELSFGFFYKEQVY 310 YR AIDL + + Y ++ I +Q R L GFF+KE VY Sbjct: 262 KYRKAIDLCVENRDAYYDVKDDLYKEIEEMQPVNRPLDTGFFFKETVY 309 >O32035.1 YRRN_BACSU Uncharacterized protease yrrN EC=3.4.-.- Length = 309 Score = 295 bits (755), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 145/288 (50%), Positives = 196/288 (68%), Gaps = 2/288 (0%) Query: 24 GADALLIGDDRFGMRLAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYVK 83 GA A L+G+ R+G+RLAG FS ED +AV++AH++G KVY ++ + N + EL Y+ Sbjct: 23 GATAFLVGEQRYGLRLAGEFSREDVTKAVEIAHKEGAKVYVAVNAIFHNDKVGELGEYLA 82 Query: 84 AIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLARE 143 + E GVD FGDPAVL A + AP LKLHW+ E T TNY T NYWGRKGA+R VLARE Sbjct: 83 FLAEAGVDAAVFGDPAVLMAARESAPDLKLHWSTETTGTNYYTCNYWGRKGAARSVLARE 142 Query: 144 LNMDEMTEMVPLLEVEAQVQVHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGKERGLFLI 203 LNMD + E+ EVE ++QVHGMT ++ SKR L+ +Y +QG+ D KE G+FL Sbjct: 143 LNMDSIVEIKENAEVEIEIQVHGMTCMFQSKRSLIGNYFEYQGKVMDIERKKKESGMFLH 202 Query: 204 EAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVAYNAAVVK 263 + ER N K+PI+EDENGTHIMS +D+CI+++L L+ AG+ S KI+G+LK Y V K Sbjct: 203 DKERDN-KYPIFEDENGTHIMSPNDVCIIDELEELIDAGIDSFKIDGVLKMPEYLIEVTK 261 Query: 264 AYRHAIDLYAADPEGY-AFQESWMDGIRALQDPERELSFGFFYKEQVY 310 YR AIDL + + Y A +E W++ I ++Q R++ GFF+KE VY Sbjct: 262 MYREAIDLCVENRDEYEAKKEDWIERIESIQPVNRKIDTGFFFKETVY 309 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,775,463,314 Number of extensions: 73447673 Number of successful extensions: 167556 Number of sequences better than 1.0e-05: 1731 Number of HSP's gapped: 165375 Number of HSP's successfully gapped: 2003 Length of query: 310 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 175 Effective length of database: 1,788,859,178 Effective search space: 313050356150 Effective search space used: 313050356150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)