BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0018.p (367 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KA32.1 B3KA32_9BACI Glucose-1-phosphate adenylyltransferase, G... 518 e-145 Q9KDX5.1 Q9KDX5_BACHD Required for glycogen biosynthesis 398 e-109 A8VWP3.1 A8VWP3_9BACI Glucose-1-phosphate adenylyltransferase, G... 395 e-108 Q8ET55.1 Q8ET55_OCEIH Glycogen biosynthesis 379 e-103 B9EB80.1 B9EB80_MACCJ Glycogen biosynthesis homolog 345 4e-93 >B3KA32.1 B3KA32_9BACI Glucose-1-phosphate adenylyltransferase, GlgD subunit EC=2.7.7.27 Length = 367 Score = 518 bits (1333), Expect = e-145, Method: Compositional matrix adjust. Identities = 240/367 (65%), Positives = 296/367 (80%) Query: 1 MKELMGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFVRRK 60 M+ELMG+INL+HE D L+ELTY+RCGAAVPFA RYRLIDFV+SNMM A +E + LFVRRK Sbjct: 1 MRELMGIINLEHERDVLSELTYYRCGAAVPFAGRYRLIDFVMSNMMHAGIEDIALFVRRK 60 Query: 61 YRSLMDHLGDGKSWDMNRKHGGLFILPPDWNDPTDTSLGDLQHYHNNLDFFKRASAKYIV 120 YRSLMDHLG+G+ WDM+RK GGLFILPPDWND TD S G+L+H +NNLDFF+R +A++IV Sbjct: 61 YRSLMDHLGEGRPWDMHRKRGGLFILPPDWNDETDVSAGELRHIYNNLDFFERGAARFIV 120 Query: 121 FSGSQHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQE 180 SGSQHI+T DL EVY+YHLE+GADVTLVY ++ + EH C R+EVDED VT+IH E Sbjct: 121 HSGSQHISTVDLNEVYEYHLEQGADVTLVYTRVNAPREEHRSCARLEVDEDGMVTDIHYE 180 Query: 181 KDHPNVYLDIFIMEKKLFLEQVEYCIAHGESYFFRDAIQKNRHKFKIAAYEYTGYHAVIN 240 H VY+++F+MEK FLE ++CIAHG + FFRDAI +NRHK IA YEY GYHAVIN Sbjct: 181 HKHDAVYMEMFVMEKSRFLEMADHCIAHGGNNFFRDAIMQNRHKLNIAGYEYLGYHAVIN 240 Query: 241 SLESYYKNSLNLLQQDNYFGLFKENPVQTKIKYEAPTRYLDSAEVSNSLVANGCIIAGTV 300 SLESYY+ S+NLL Y LF++ V TKIKYE P +YL+SA+VSN+LVANGC I G V Sbjct: 241 SLESYYRTSMNLLNPKAYNSLFRKQAVHTKIKYEVPAKYLNSADVSNALVANGCQIDGEV 300 Query: 301 ENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVGDSKRPFV 360 +NS++FRGVQ+++GA I NS+IMQKC+I EN +ENVI+DKDV +SR+ L GD ++PFV Sbjct: 301 KNSILFRGVQVKRGASIHNSIIMQKCLIGENVRLENVILDKDVIISRDHKLQGDFRKPFV 360 Query: 361 IAKSSKI 367 IAK I Sbjct: 361 IAKDISI 367 >Q9KDX5.1 Q9KDX5_BACHD Required for glycogen biosynthesis Length = 368 Score = 398 bits (1023), Expect = e-109, Method: Compositional matrix adjust. Identities = 187/364 (51%), Positives = 264/364 (72%), Gaps = 1/364 (0%) Query: 1 MKELMGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFVRRK 60 MK LMGV+NLDH+ D L+ELTYFR A VPF RYR+IDFVLSNM+ + ++ + +F R K Sbjct: 1 MKSLMGVVNLDHDQDLLHELTYFRSVAGVPFGGRYRMIDFVLSNMVNSGIQDIAVFARNK 60 Query: 61 YRSLMDHLGDGKSWDMNRKHGGLFILPPDWNDPTDTSLGDLQHYHNNLDFFKRASAKYIV 120 YRSLMDHLG G WD+NRK GGLFILPPDWNDPTD S GDL+H+HN+ DFF R +A +++ Sbjct: 61 YRSLMDHLGTGAPWDLNRKRGGLFILPPDWNDPTDISKGDLRHFHNHRDFFHRGNADHVL 120 Query: 121 FSGSQHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQE 180 SGSQ I+ D ++ ++ H E ADVT++YKK+++L+PE +++E D NV I + Sbjct: 121 VSGSQFISKVDYEDAFKRHQETKADVTVLYKKVDQLEPEDRYRMKLEFDSFGNVKSITND 180 Query: 181 KDHPNVYLDIFIMEKKLFLEQVEYCIAHGESYFFRDAIQKNRHKFKIAAYEYTGYHAVIN 240 + + N+Y+ ++I+ K+L LE + +CI + + F D I N ++ I AYEY GY ++I+ Sbjct: 181 EMNSNLYMQMYIIRKELLLELLNHCIGYHKQDLFVDGIMSNLNELAIRAYEYKGYLSIIS 240 Query: 241 SLESYYKNSLNLLQQDNYFGLF-KENPVQTKIKYEAPTRYLDSAEVSNSLVANGCIIAGT 299 S+ SYYK+S++LL+ + Y LF +P+ TKIK E P +Y++ +EVSNSLVANGC+I G Sbjct: 241 SVASYYKHSMDLLKPEIYQSLFLAGSPIYTKIKDEPPAKYVNGSEVSNSLVANGCVIEGH 300 Query: 300 VENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVGDSKRPF 359 VENS++FRGV + KG I NS+IMQ+C I E+ +++VI+DKD+ +S R G ++PF Sbjct: 301 VENSILFRGVYVAKGVSIKNSIIMQRCYIHEDTHLDHVILDKDILISPNRQFAGAPEQPF 360 Query: 360 VIAK 363 VIAK Sbjct: 361 VIAK 364 >A8VWP3.1 A8VWP3_9BACI Glucose-1-phosphate adenylyltransferase, GlgD subunit EC=2.7.7.27 Length = 390 Score = 395 bits (1014), Expect = e-108, Method: Compositional matrix adjust. Identities = 180/368 (48%), Positives = 262/368 (71%), Gaps = 1/368 (0%) Query: 1 MKELMGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFVRRK 60 M+ L+G+INLD+E D L ELTYFRCGAA PFA RYR+IDF LSNM+ + +E V +F K Sbjct: 23 MEPLLGLINLDNEHDFLEELTYFRCGAATPFAGRYRMIDFTLSNMVNSGVEEVAIFTNHK 82 Query: 61 YRSLMDHLGDGKSWDMNRKHGGLFILPPDWNDPTDTSLGDLQHYHNNLDFFKRASAKYIV 120 YR+LMDH+ G W++ R+HG +FILPPDWNDPTD S GDL+H+HNN DFF R+ A+ ++ Sbjct: 83 YRALMDHIKTGADWELARRHGRVFILPPDWNDPTDISRGDLRHFHNNRDFFHRSKAENVI 142 Query: 121 FSGSQHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQE 180 SGSQ++ + + Y+YHLE+ ADVT++ + E EH LR+E DED V + Sbjct: 143 ISGSQYLTNVNALKAYEYHLERDADVTVIQTNVPEPGDEHRQLLRIESDEDGWVKGFSSD 202 Query: 181 KDHPNVYLDIFIMEKKLFLEQVEYCIAHGESYFFRDAIQKNRHKFKIAAYEYTGYHAVIN 240 + V+ +++I++K++ L+ V++CIA+ + FF D ++ KI Y + GY IN Sbjct: 203 PANSKVFTNVYIIKKEVLLQLVDFCIAYHKDNFFLDGVRGKLPDLKIQHYSFNGYGMFIN 262 Query: 241 SLESYYKNSLNLLQQDNYFGLFKENP-VQTKIKYEAPTRYLDSAEVSNSLVANGCIIAGT 299 S++SYYKN+L+LLQ DNY LF +N ++TK+ E P +Y++ + V+ S+V+NGC+I GT Sbjct: 263 SIQSYYKNNLSLLQPDNYKKLFFKNQLIKTKLSNEPPAKYVNGSAVTKSMVSNGCVIEGT 322 Query: 300 VENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVGDSKRPF 359 V+NSV+FRGV+I A + NS+IMQ+CVIE+N +ENVI+DKDVH+S + L+G +P+ Sbjct: 323 VDNSVLFRGVKISPTATVKNSIIMQRCVIEDNVHLENVILDKDVHVSAGQQLIGSKDKPY 382 Query: 360 VIAKSSKI 367 V+AK ++ Sbjct: 383 VVAKRQRV 390 >Q8ET55.1 Q8ET55_OCEIH Glycogen biosynthesis Length = 368 Score = 379 bits (972), Expect = e-103, Method: Compositional matrix adjust. Identities = 175/368 (47%), Positives = 262/368 (71%), Gaps = 1/368 (0%) Query: 1 MKELMGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFVRRK 60 M+ +MG++NL+HE LNEL YFR A++PF RYRLIDF LSNM+ + ++ V +FVR K Sbjct: 1 MERMMGLVNLEHEHHLLNELAYFRNPASIPFGGRYRLIDFTLSNMVNSSIQEVAIFVRNK 60 Query: 61 YRSLMDHLGDGKSWDMNRKHGGLFILPPDWNDPTDTSLGDLQHYHNNLDFFKRASAKYIV 120 YRSL+DH+G G++W+++R++GGLFILPPDWNDPTD S GDL+ +HNN D+F R+++KY++ Sbjct: 61 YRSLLDHIGTGENWELDRRNGGLFILPPDWNDPTDRSRGDLKFFHNNRDYFNRSNSKYVL 120 Query: 121 FSGSQHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQE 180 SGSQ I+ D +E + H+++ ADVTL+ + EEL EH+ LRVE D V + + Sbjct: 121 ISGSQFISNTDYKEALEQHIKEQADVTLITVREEELSNEHESNLRVECDYIHRVQALTND 180 Query: 181 KDHPNVYLDIFIMEKKLFLEQVEYCIAHGESYFFRDAIQKNRHKFKIAAYEYTGYHAVIN 240 K++P ++ ++I+ K L +E V+ CIA+ + +FF AI + ++ + GYHA IN Sbjct: 181 KNNPEIFTGVYIINKDLLIELVDNCIAYHKDHFFHAAIMERLGDLRVFRFRQEGYHAFIN 240 Query: 241 SLESYYKNSLNLLQQDNYFGLFKENP-VQTKIKYEAPTRYLDSAEVSNSLVANGCIIAGT 299 S+ES+YK+++ LLQ+D+Y LF N V+TKI AP +Y + AEV N+LVANGC + G Sbjct: 241 SVESFYKHNMKLLQEDSYQQLFLNNHFVRTKISNNAPVKYGEKAEVVNTLVANGCKVNGC 300 Query: 300 VENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVGDSKRPF 359 +E S++FRGVQ+ +GA + NS+IMQ+C I +ENVI+DKDV ++ + ++G ++P+ Sbjct: 301 IEGSILFRGVQLHEGATVKNSIIMQRCTIGSGVHLENVILDKDVVVTTGQRIIGSKEKPY 360 Query: 360 VIAKSSKI 367 V+AK I Sbjct: 361 VVAKRQVI 368 >B9EB80.1 B9EB80_MACCJ Glycogen biosynthesis homolog Length = 367 Score = 345 bits (885), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 168/368 (45%), Positives = 249/368 (67%), Gaps = 2/368 (0%) Query: 1 MKELMGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFVRRK 60 M+ LMG+INL +E D L ELTYFR GA+VPFA RYRLIDF LSN++ + + V +FV +K Sbjct: 1 MRSLMGLINLSNEQDYLEELTYFRNGASVPFAGRYRLIDFSLSNIVSSGTDQVAIFVNQK 60 Query: 61 YRSLMDHLGDGKSWDMNRKHGGLFILPPDWNDPTDTSLGDLQHYHNNLDFFKRASAKYIV 120 YRSLMDHLG+G + ++ + LF+LPPDW+DP+D S GDL+H+HNN DFF R++A IV Sbjct: 61 YRSLMDHLGNGDDFGLDDRKSRLFVLPPDWHDPSDISRGDLRHFHNNRDFFIRSNATDIV 120 Query: 121 FSGSQHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQE 180 SGSQ I D ++ +++H+E AD+TL+ + ++EL+ H P LR+ +E S VT ++ + Sbjct: 121 ISGSQMIVNTDFKDAFKFHIENDADITLISENVDELEEMHQPLLRIN-NEGSKVTYLNHD 179 Query: 181 KDHPNVYLDIFIMEKKLFLEQVEYCIAHGESYFFRDAIQKNRHKFKIAAYEYTGYHAVIN 240 + +V+ +I++K E ++YCI + + FF I + + + Y+ IN Sbjct: 180 SANAHVFTGTYIIKKDKLFEIMDYCIQNYKDNFFFHGIFERMGELNVTYYDIQEVTMYIN 239 Query: 241 SLESYYKNSLNLLQQDNYFGLF-KENPVQTKIKYEAPTRYLDSAEVSNSLVANGCIIAGT 299 SL+++YKN+L++L + Y +F KEN ++TKI + PT+Y+ V NS+VANGC IAG Sbjct: 240 SLKTFYKNNLSILDPERYKAIFMKENRIKTKISNQPPTKYIGDCTVHNSIVANGCEIAGH 299 Query: 300 VENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVGDSKRPF 359 VENS+++RGV + G I NS+IM C I+ A+IENVI+DKDV ++ + L+G +PF Sbjct: 300 VENSILYRGVCVEPGVTIKNSIIMTNCQIKAGALIENVILDKDVIVNEHQKLIGSQDKPF 359 Query: 360 VIAKSSKI 367 V+AK I Sbjct: 360 VVAKRQTI 367 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,141,184,645 Number of extensions: 90074283 Number of successful extensions: 214303 Number of sequences better than 1.0e-05: 1288 Number of HSP's gapped: 211151 Number of HSP's successfully gapped: 1321 Length of query: 367 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 230 Effective length of database: 1,770,017,148 Effective search space: 407103944040 Effective search space used: 407103944040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)