BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0010.p (432 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6CHJ5.1 A6CHJ5_9BACI Putative uncharacterized protein 273 2e-71 O31561.1 YFIS_BACSU Uncharacterized MFS-type transporter yfiS 260 2e-67 C1PFG3.1 C1PFG3_BACCO Major facilitator superfamily MFS_1 240 2e-61 C0Z5U2.1 C0Z5U2_BREBN Conserved hypothetical membrane protein 164 2e-38 C2PYS4.1 C2PYS4_BACCE Major facilitator superfamily MFS_1 150 2e-34 >A6CHJ5.1 A6CHJ5_9BACI Putative uncharacterized protein Length = 423 Score = 273 bits (699), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 152/418 (36%), Positives = 247/418 (59%), Gaps = 9/418 (2%) Query: 13 KLTSNKPFVLLITAQLVSNIGDWLHILALLTMVGLKWQASPWEITAVSLCMAVPMLLGGP 72 KL NK F+ L+TAQ +S++GDWL I+A++T+VGLKW+A+P +++ + L +A+PM L GP Sbjct: 4 KLGQNKNFITLMTAQAISSLGDWLSIVAIITLVGLKWEATPLQMSLLILSLALPMALLGP 63 Query: 73 FAGYLSDKVDRKGIMIGSDLVRAGIVACLVFAGSLWQVYVLLLAKGVMDVLFSPAKSGKL 132 +G ++D+++RK +MI SD+ R ++ L A ++W VY L A G+ +F PAK+GKL Sbjct: 64 VSGAVADRMERKTLMIISDVTRGILILFLTLASNVWAVYACLFAIGIFSSVFVPAKNGKL 123 Query: 133 KELVPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAVGISACYFIDSVSYIASAVILLG 192 KELV + A++++S I+ TK++GP + G+LV+AVG ++IDS ++ SAV++L Sbjct: 124 KELVRDQNIKGAMSITSMIDSSTKVLGPLVSGILVSAVGTYNVFYIDSATFFLSAVLILF 183 Query: 193 LPRVGMFKTGRPNEDGDTARPSFRKEAGAGLRLIGSMPVVLSGVVMIVAALLVLQIADSQ 252 LP+ + P ++ + +FR+E G+ I ++ G+ ++ LL+LQ++DSQ Sbjct: 184 LPKTQKEISTSPAQEKQES--TFREELKEGISFIRQSRYLMYGLFLLGVTLLILQLSDSQ 241 Query: 253 TVTLFREIPGVNADLLGWCVAASGFGTLLSALAVGRLGSGKHPLVFMGTGTLLMGSVFAG 312 + L R++ V+ DL G+ V +SG G LL+ + + + + + ++M G L +G F Sbjct: 242 IIILLRQLTDVSPDLFGYAVTSSGLGMLLTGIYLSK-KTDYNSYLYMCFGVLGIGMGFGI 300 Query: 313 AALVTV--TGQAGFWMDTMLFGSFVLAGVGAGFVIIPFNSMLQRRTPEAYTGRVFGIVGS 370 AL+T T + W+ + ++AGV AG V IPF + Q +TP TGRVFG++ S Sbjct: 301 MALLTYFDTVHSIVWVPLLA----LIAGVAAGLVFIPFQAATQEKTPVHMTGRVFGVINS 356 Query: 371 LTSAAVILGPVAGGALVTASGPATXXXXXXXXXXXXXXXXXXXRTGIENRDQAAQELQ 428 T+ A I+GP+AGGAL T G R +E D + Q Sbjct: 357 TTTTATIIGPLAGGALATLIGIIPSFLVTSSLLIILFLVSVIFRNKVEQEDHHVTKSQ 414 >O31561.1 YFIS_BACSU Uncharacterized MFS-type transporter yfiS Length = 417 Score = 260 bits (664), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 148/371 (39%), Positives = 219/371 (59%), Gaps = 8/371 (2%) Query: 16 SNKPFVLLITAQLVSNIGDWLHILALLTMVGLKWQASPWEITAVSLCMAVPMLLGGPFAG 75 + K + L+ +Q +S++GDWLHILA+LT+ + ASP +++ + + A+P+++ GP +G Sbjct: 8 NQKGLMTLLASQTISSLGDWLHILAVLTLAAFQLHASPLDMSLLMMSFALPVIVLGPVSG 67 Query: 76 YLSDKVDRKGIMIGSDLVRAGIVACLVFAGSLWQVYVLLLAKGVMDVLFSPAKSGKLKEL 135 L+D+ DRK IM S++ RA V V+ LWQ+YVLL + LF PAK+GKLKEL Sbjct: 68 LLADRFDRKTIMFLSEIGRALTVISCVYVSELWQLYVLLSVQSCFSSLFLPAKNGKLKEL 127 Query: 136 VPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAVGISACYFIDSVSYIASAVILLGLPR 195 P + QAV++SS I+ +KI GPALGG L+AA I + ++I++ ++ SAVIL LPR Sbjct: 128 APEAHIQQAVSVSSIIDNSSKIFGPALGGTLIAAFSIHSVFYINAGAFFLSAVILFFLPR 187 Query: 196 VGMFKTGRPNEDGDTARPSFRKEAGAGLRLIGSMPVVLSGVVMIVAALLVLQIADSQTVT 255 + ++ A S ++ GL+ + MP++L+G++ L VLQI DSQ + Sbjct: 188 DAFLQKANTPQEKTAALTSIKE----GLQFLKRMPLLLTGLLTACVVLFVLQIGDSQAII 243 Query: 256 LFREIPGVNADLLGWCVAASGFGTLLSALAVGRLGSGKHPLVFMGTGTLLMGSVFAGAAL 315 L R G +L GWC+A SG G LL+A GR + L++ GTLL+G GA Sbjct: 244 LIRSFSGAPPELAGWCMAVSGAGMLLTAAITGRRRITSY-LLYFSAGTLLLGLATGGAPF 302 Query: 316 VTVTGQAGFWMDTMLFGSFVLAGVGAGFVIIPFNSMLQRRTPEAYTGRVFGIVGSLTSAA 375 ++ G AG T+ +F + G G V IPF ++Q P Y+GRVFG + S T+ A Sbjct: 303 LSGMGIAGI---TLFIFAFFIMGAAFGLVHIPFQILVQTTVPVDYSGRVFGAIQSATTLA 359 Query: 376 VILGPVAGGAL 386 ILG GG L Sbjct: 360 SILGMAGGGVL 370 >C1PFG3.1 C1PFG3_BACCO Major facilitator superfamily MFS_1 Length = 415 Score = 240 bits (613), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 134/380 (35%), Positives = 209/380 (55%), Gaps = 22/380 (5%) Query: 14 LTSNKPFVLLITAQLVSNIGDWLHILALLTMVGLKWQASPWEITAVSLCMAVPMLLGGPF 73 L N F+ LI+AQ VSN+GDWLH++AL +V KW A P + ++LCM +P +L G Sbjct: 5 LFKNHSFLFLISAQTVSNLGDWLHLVALFALVAFKWHADPIAMAGITLCMVLPSILFGSP 64 Query: 74 AGYLSDKVDRKGIMIGSDLVRAGIVACLVFAGSLWQVYVLLLAKGVMDVLFSPAKSGKLK 133 AG+L+D+ +RK +M SD R G V + F+ SLWQVY+ L G +F+PA+SG L+ Sbjct: 65 AGWLADRFNRKVLMSFSDFARCGCVLGIAFSVSLWQVYIFLFFLGFFSAVFTPAESGLLR 124 Query: 134 ELVPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAVGISACYFIDSVSYIASAVILLGL 193 ++V Q+ A+ S I KIIGP GG L++ GI +++D++S+ SA++L G+ Sbjct: 125 QVVGENQIQAAIGTSEMINNSAKIIGPVAGGALISLTGIKGAFYLDAISFFLSALLLFGI 184 Query: 194 PRVGMFKTGRPNEDGDTARPSFRKEAGA---GLRLIGSMPVVLSGVVMIVAALLVLQIAD 250 P +E A G R + PV+ G+++ +L LQI+D Sbjct: 185 K--------APTLQSPVKSDLEHREKVALTEGFRFLSGFPVLKMGLIVFCTMILALQISD 236 Query: 251 SQTVTLFREIPGVNADLLGWCVAASGFGTLLSALAVGRLGSGKHPLVFMGTGTLLMGSVF 310 SQ + L R+I WC+AASGFG L +++ ++ G+ + TL + Sbjct: 237 SQAMILIRDIKNATVHFASWCIAASGFGMLTASVLFTKIKLGEKLI------TLKISPAI 290 Query: 311 AGAALVTVTGQAGFW----MDTMLFGSFVLAGVGAGFVIIPFNSMLQRRTPEAYTGRVFG 366 G + V+ G W ++ + F L G IPF+ ++Q++TPE +TGRVFG Sbjct: 291 LGLGCIMVSTGTG-WPIGIIEAVYPCVFFLMGFSFTMAAIPFDVLVQKKTPETHTGRVFG 349 Query: 367 IVGSLTSAAVILGPVAGGAL 386 + SL++ AV++G + GG+L Sbjct: 350 TINSLSTLAVLVGILLGGSL 369 >C0Z5U2.1 C0Z5U2_BREBN Conserved hypothetical membrane protein Length = 418 Score = 164 bits (415), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 116/383 (30%), Positives = 199/383 (51%), Gaps = 12/383 (3%) Query: 14 LTSNKPFVLLITAQLVSNIGDWLHILALLTMVGLKWQASPWEITAVSLCMAVPMLLGGPF 73 L N+ F L + +S +GDW +A+ ++G W+ P + + + A P +L G F Sbjct: 2 LKENRTFRTLFVSYGLSTLGDWFDFIAVSILLGFVWKVDPMTMALLPVAYAAPSILLGQF 61 Query: 74 AGYLSDKVDRKGIMIGSDLVRAGI-VACLVFAGSLWQVYVLLLAKGVMDVLFSPAKSGKL 132 AG L+D+V++ +++ ++++A + LV W + V+ L G V PA+ Sbjct: 62 AGVLADRVNKVRMIMSMNIMQAAFTLLLLVMPNPFWFLVVIALRSGA-SVFNDPAQQTLT 120 Query: 133 KELVPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAVGISACYFIDSVSYIASAVILLG 192 +++VP Q+ QA AL+ ++ Q+ K+IGP GGL+ A + C I++ +++ S L Sbjct: 121 RQIVPKNQLLQASALNGAVYQMGKLIGPLAGGLVAAVFAPAICLMINASTFLLSTGFLFT 180 Query: 193 LPRVGMFKT-GRPNEDGDTARPSFRKEAGAGLRLIGSMPVVLSGVVMIVAALLVLQIADS 251 + +V KT RP+ + + P +R+ G R+ V+L + + L +Q+AD+ Sbjct: 181 IRKVE--KTLSRPSVE-EQPLP-YRQAWQEGWRIFLQNRVLLYSTIFAIITSLAIQLADT 236 Query: 252 QTVTLFREIPGVNADLLGWCVAASGFGTLLSALAVGRLGSGKHPLVFMGTGTLLMGSVFA 311 Q +FRE N ++LG+ V+ G G L++ + RL + +G G LL+G F+ Sbjct: 237 QFPVIFREKLPDNPEMLGFTVSVIGLGALVTVTWLHRLKEIRSYGWVLGGGVLLIGISFS 296 Query: 312 GAALVTVTGQAGFWMDTMLFGSFVLAGVGAGFVIIPFNSMLQRRTPEAYTGRVFGIVGSL 371 G +W L + +LAGVG G + N ++Q+ +P GRV GI+ SL Sbjct: 297 WIGFFQ-PGDGMYW----LLIAALLAGVGTGLTSVGANYLVQKESPPQALGRVRGIIDSL 351 Query: 372 TSAAVILGPVAGGALVTASGPAT 394 TSA I+ P+ GG ++T GP T Sbjct: 352 TSATFIIAPLLGGVIMTIWGPNT 374 >C2PYS4.1 C2PYS4_BACCE Major facilitator superfamily MFS_1 Length = 419 Score = 150 bits (379), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 114/382 (29%), Positives = 195/382 (51%), Gaps = 15/382 (3%) Query: 14 LTSNKPFVLLITAQLVSNIGDWLHILALLTMVGLKWQASPWEITAVSLCMAVPMLLGGPF 73 L N+ +V + +A +S G +L ++A+ T+V W++ P I+ + + A+PM+L G + Sbjct: 7 LWGNRIYVRMFSAFSISIFGVYLDMIAVATLVSYTWKSDPIIISLIPIAYALPMILFGSW 66 Query: 74 AGYLSDKVDRKGIMIGSDLVRAGIVACLVFAGSLWQVYVLLLAKGVMDVLFSPAKSGKLK 133 AG LSD++ + IMI +D++ A + LVF +++ + +LL + F PA K Sbjct: 67 AGVLSDRLKKLRIMIIADVLIAIVTFILVFVPNVYWLLLLLAVRSTFASFFQPAHQALTK 126 Query: 134 ELVPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAVGISACYFIDSVSYIASAVILLGL 193 ++V + QA + + ++Q+ +I GP LG +L+ + + VS + SA ILL + Sbjct: 127 QVVHEHHLAQATSWNGLMDQVGRIAGPLLGAMLITGLSPQISMSVRGVSSLCSAFILLSI 186 Query: 194 PRVGMFKTGRPNED-GDTARPSFRKEAGAGLRLIGSMPVVLSGVVMIVAALLVLQIADSQ 252 + M K + E T R F G + + + ++ + + + +LQ+ +SQ Sbjct: 187 SK--MVKDKKQFEQIQKTERQGFFSAWLEGWKYVWNRKIIFYTMFYGLCGVGILQLVESQ 244 Query: 253 TVTLFREIPGVNADLLGWCVAASGFGTLLSALAVGRLGSGKHPLVFMGTGTLLMGSVFAG 312 L RE+ + +++GW A G G + L + R+G K+ F+G G L+G F Sbjct: 245 VPVLMRELFPNSPEMVGWINAIVGLGGVCGVLLIKRIGELKYGW-FLGGGIALIGLGFGS 303 Query: 313 AALVTVTGQAGFWMDTMLFGSFV---LAGVGAGFVIIPFNSMLQRRTPEAYTGRVFGIVG 369 L T T T +F +FV +AG+G+G I FN +LQ+ T GRVFGI Sbjct: 304 LGLFTET--------TPVFFTFVIGFIAGIGSGLFFISFNIILQKETDIETAGRVFGIQS 355 Query: 370 SLTSAAVILGPVAGGALVTASG 391 SL++ I+ P+ GG V G Sbjct: 356 SLSNLMFIVPPLIGGVFVKLIG 377 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,394,543,768 Number of extensions: 104287154 Number of successful extensions: 399511 Number of sequences better than 1.0e-05: 3633 Number of HSP's gapped: 395814 Number of HSP's successfully gapped: 3635 Length of query: 432 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 293 Effective length of database: 1,751,175,118 Effective search space: 513094309574 Effective search space used: 513094309574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)