BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0004.p (267 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K553.1 B3K553_9BACI NH(3)-dependent NAD(+) synthetase EC=6.3.1.5 403 e-111 B1D657.1 B1D657_9BACL NH(3)-dependent NAD(+) synthetase EC=6.3.1.5 384 e-105 A0Y0H0.1 A0Y0H0_9GAMM NH(3)-dependent NAD(+) synthetase EC=6.3.1.5 207 1e-51 B2GA98.1 NADE_LACF3 NH(3)-dependent NAD(+) synthetase EC=6.3.1.5 202 3e-50 C0WVF6.1 C0WVF6_LACFE NAD(+) synthase EC=6.3.1.5 202 3e-50 >B3K553.1 B3K553_9BACI NH(3)-dependent NAD(+) synthetase EC=6.3.1.5 Length = 269 Score = 403 bits (1035), Expect = e-111, Method: Compositional matrix adjust. Identities = 192/267 (71%), Positives = 221/267 (82%) Query: 1 MQSEIIAALGVKPSIDVEAEVRKRVDFLKAYVLEXXXXXXXXXXXXXVDSAVAAGLCKRA 60 +Q +IIA LGVKP+I+VE EVRKRVDFLK YV +DSAVAAGLCKRA Sbjct: 3 LQEQIIAELGVKPTINVEEEVRKRVDFLKNYVKNSGTSGLLIAISGGIDSAVAAGLCKRA 62 Query: 61 TDELTAEEGKDYMTLGVFQPYGEQEDIEHSYAVAEAFGLTHRAETNIEEAVNEIALEVEH 120 TDELT E GK+Y TLGVFQPYGEQ DI SYA A+AF L + ETNIEEAVNE+ALEVEH Sbjct: 63 TDELTEENGKEYKTLGVFQPYGEQADISDSYATAKAFDLKYTVETNIEEAVNEVALEVEH 122 Query: 121 SLKSLGQHRHITHQGKGNVKARTRMVMQYALAFENNLLVVGTDHASEAITGFYTKWGDGA 180 LK++G H+H++ GKGNVKAR RMV+QYALAFE NL+VVGTDHASEAITGFYTKWGDGA Sbjct: 123 GLKNIGVHQHMSIPGKGNVKARVRMVVQYALAFEQNLIVVGTDHASEAITGFYTKWGDGA 182 Query: 181 VDITPLSSLNKRQVRQLAAFLGVPADIVTKAPTAGLWPGQTDEAELGITYEENSDYLEGK 240 VDITPLSSLNKRQVR LA++LGVP I+ KAP+AGLW GQTDE ELGI+Y++NSDYLEGK Sbjct: 183 VDITPLSSLNKRQVRMLASYLGVPQSILDKAPSAGLWEGQTDEKELGISYDDNSDYLEGK 242 Query: 241 TVSPEAAKKLENFYRRTAHKRNVIPGI 267 T++PE +KLE++Y +TAHKRN IPGI Sbjct: 243 TINPEVQQKLESYYVKTAHKRNTIPGI 269 >B1D657.1 B1D657_9BACL NH(3)-dependent NAD(+) synthetase EC=6.3.1.5 Length = 269 Score = 384 bits (985), Expect = e-105, Method: Compositional matrix adjust. Identities = 188/267 (70%), Positives = 212/267 (79%) Query: 1 MQSEIIAALGVKPSIDVEAEVRKRVDFLKAYVLEXXXXXXXXXXXXXVDSAVAAGLCKRA 60 +Q EII GV+P ID AE+RKRVDFLK YV + +DSAV GLCK A Sbjct: 3 LQQEIIERFGVRPEIDPAAEIRKRVDFLKEYVKKSGTTGLLIAISGGIDSAVTTGLCKMA 62 Query: 61 TDELTAEEGKDYMTLGVFQPYGEQEDIEHSYAVAEAFGLTHRAETNIEEAVNEIALEVEH 120 TDEL+AE G++YMTLGVFQPYGEQ DIE SYA AEAF L H+ ETNI EAV+EIALE EH Sbjct: 63 TDELSAETGREYMTLGVFQPYGEQVDIEDSYATAEAFQLKHKVETNIGEAVDEIALETEH 122 Query: 121 SLKSLGQHRHITHQGKGNVKARTRMVMQYALAFENNLLVVGTDHASEAITGFYTKWGDGA 180 +LK++G HRH++ GKGNVKARTRMV+QYALAF+ NLLVVGTDHASEAITGF+TKWGDGA Sbjct: 123 ALKAIGIHRHLSRGGKGNVKARTRMVVQYALAFDLNLLVVGTDHASEAITGFFTKWGDGA 182 Query: 181 VDITPLSSLNKRQVRQLAAFLGVPADIVTKAPTAGLWPGQTDEAELGITYEENSDYLEGK 240 VDITPLSSLNKRQVRQLA+ LGVP I+ KAPTAGLW GQTDE+ELGI Y+ENS YLEGK Sbjct: 183 VDITPLSSLNKRQVRQLASVLGVPRSILDKAPTAGLWEGQTDESELGILYDENSAYLEGK 242 Query: 241 TVSPEAAKKLENFYRRTAHKRNVIPGI 267 + EA KLE Y RT HKR+ IPGI Sbjct: 243 EIGTEAKAKLEKQYLRTEHKRSPIPGI 269 >A0Y0H0.1 A0Y0H0_9GAMM NH(3)-dependent NAD(+) synthetase EC=6.3.1.5 Length = 276 Score = 207 bits (526), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 121/276 (43%), Positives = 162/276 (58%), Gaps = 11/276 (3%) Query: 1 MQSEIIAALGVKPSIDVEAEVRKRVDFLKAYVLEXXXXXXXXXXXXXVDSAVAAGLCKRA 60 M++EI+A + V+P+IDV AE+ +RV+F+KA ++ VDS+ LC+ A Sbjct: 1 MRAEIMAEMKVQPTIDVNAEISRRVNFIKARLVAAHSRSLVLGISGGVDSSTCGRLCQLA 60 Query: 61 TDELTAEEGKD-YMTLGVFQPYGEQEDIEHSYAVAEAFGLTHRAETNIEEAVNEIALEVE 119 +EL E + Y + V PYG Q D + + + + R NI+ A + + +V Sbjct: 61 VNELNEEHSTNKYQFIAVRLPYGVQADEDEAQMAVDFIQPSKRMTVNIQPAADALHEQVL 120 Query: 120 HSLKSLGQHRHITHQG-----KGNVKARTRMVMQYALAFENNLLVVGTDHASEAITGFYT 174 ++ S G + Q KGNVKAR RMV QY +A + LVVGTDH++E ITGFYT Sbjct: 121 IAMTSSGDA--LPEQAQIDFIKGNVKARQRMVSQYEIAGFSQGLVVGTDHSAENITGFYT 178 Query: 175 KWGDGAVDITPLSSLNKRQVRQLAAFLGVPADIVTKAPTAGL---WPGQTDEAELGITYE 231 K+GDGA D+ PL L+KRQVR LA LG PA +V KAPTA L PG TDE LG++Y Sbjct: 179 KFGDGACDLAPLFGLSKRQVRALATALGAPAVLVEKAPTADLESDRPGLTDEEALGLSYN 238 Query: 232 ENSDYLEGKTVSPEAAKKLENFYRRTAHKRNVIPGI 267 + D+LEGK VS E +KL Y RT HKR IP I Sbjct: 239 QIDDFLEGKPVSEEVEQKLIGIYLRTQHKRQPIPTI 274 >B2GA98.1 NADE_LACF3 NH(3)-dependent NAD(+) synthetase EC=6.3.1.5 Length = 276 Score = 202 bits (514), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 115/268 (42%), Positives = 161/268 (60%), Gaps = 11/268 (4%) Query: 1 MQSEIIAALGVKPSIDVEAEVRKRVDFLKAYVLEXXXXXXXXXXXXXVDSAVAAGLCKRA 60 +Q +II L V P+ID + EVR+R+DFLK Y+ + DSA+A L + A Sbjct: 4 LQKQIIEDLKVIPTIDPQVEVRRRIDFLKDYLKQTKMATLVLGISGGQDSALAGRLAQLA 63 Query: 61 TDELTAEEG-KDYMTLGVFQPYGEQEDIEHSYAVAEAFGLTHRA-ETNIEEAVNEIALEV 118 +EL E G +DY + V PYGEQ D + + F R + NI+ A + + + + Sbjct: 64 VEELRKESGSEDYQFIAVRLPYGEQADESDAMMAIDDFIHPDRVVKVNIKPATDAMVMTL 123 Query: 119 EHSLKSLGQHRHITHQGKGNVKARTRMVMQYALAFENNLLVVGTDHASEAITGFYTKWGD 178 E + I+ KGN+KAR RM++QYA+A E + VVGTDHA+EA+TGFYTK+GD Sbjct: 124 EAA------GTKISDFNKGNIKARERMIVQYAIAGEYHGAVVGTDHAAEAVTGFYTKYGD 177 Query: 179 GAVDITPLSSLNKRQVRQLAAFLGVPADIVTKAPTAGL---WPGQTDEAELGITYEENSD 235 G D+TPLS L+KRQ R L +LG P + K PTA L P DE LG+TY++ D Sbjct: 178 GGADVTPLSQLDKRQGRALLEYLGAPEKLYQKTPTADLEEDRPALPDEQALGVTYKDIDD 237 Query: 236 YLEGKTVSPEAAKKLENFYRRTAHKRNV 263 +LEG+ V AA+K+E +Y+RT HKR++ Sbjct: 238 FLEGREVDQAAAEKIEAWYQRTGHKRHM 265 >C0WVF6.1 C0WVF6_LACFE NAD(+) synthase EC=6.3.1.5 Length = 276 Score = 202 bits (514), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 115/268 (42%), Positives = 161/268 (60%), Gaps = 11/268 (4%) Query: 1 MQSEIIAALGVKPSIDVEAEVRKRVDFLKAYVLEXXXXXXXXXXXXXVDSAVAAGLCKRA 60 +Q +II L V P+ID + EVR+R+DFLK Y+ + DSA+A L + A Sbjct: 4 LQEQIIEDLKVIPTIDPQVEVRRRIDFLKDYLKQTKMATLVLGISGGQDSALAGRLAQLA 63 Query: 61 TDELTAEEG-KDYMTLGVFQPYGEQEDIEHSYAVAEAFGLTHRA-ETNIEEAVNEIALEV 118 +EL E G +DY + V PYGEQ D + + F R + NI+ A + + + + Sbjct: 64 VEELRKESGSEDYQFIAVRLPYGEQADESDAMMAIDDFIHPDRVVKVNIKPATDAMVMTL 123 Query: 119 EHSLKSLGQHRHITHQGKGNVKARTRMVMQYALAFENNLLVVGTDHASEAITGFYTKWGD 178 E + I+ KGN+KAR RM++QYA+A E + VVGTDHA+EA+TGFYTK+GD Sbjct: 124 EAA------GTKISDFNKGNIKARERMIVQYAIAGEYHGAVVGTDHAAEAVTGFYTKYGD 177 Query: 179 GAVDITPLSSLNKRQVRQLAAFLGVPADIVTKAPTAGL---WPGQTDEAELGITYEENSD 235 G D+TPLS L+KRQ R L +LG P + K PTA L P DE LG+TY++ D Sbjct: 178 GGADVTPLSQLDKRQGRALLEYLGAPEKLYQKTPTADLEEDRPALPDEQALGVTYKDIDD 237 Query: 236 YLEGKTVSPEAAKKLENFYRRTAHKRNV 263 +LEG+ V AA+K+E +Y+RT HKR++ Sbjct: 238 FLEGREVDQAAAEKIEAWYQRTGHKRHM 265 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,443,902,536 Number of extensions: 55435004 Number of successful extensions: 115060 Number of sequences better than 1.0e-05: 1045 Number of HSP's gapped: 113855 Number of HSP's successfully gapped: 1045 Length of query: 267 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 134 Effective length of database: 1,807,701,208 Effective search space: 242231961872 Effective search space used: 242231961872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 130 (54.7 bits)