BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0022.p (503 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9R3.1 B3K9R3_9BACI Glycoside hydrolase family 4 910 0.0 B1DD65.1 B1DD65_9BACL Glycoside hydrolase family 4 851 0.0 Q5WL01.1 Q5WL01_BACSK Alpha-galactosidase EC=3.2.1.22 771 0.0 B8I9A4.1 B8I9A4_CLOCE Glycoside hydrolase family 4 686 0.0 Q2CK17.1 Q2CK17_9RHOB Putative uncharacterized protein 614 e-174 >B3K9R3.1 B3K9R3_9BACI Glycoside hydrolase family 4 Length = 499 Score = 910 bits (2351), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/500 (85%), Positives = 463/500 (92%), Gaps = 1/500 (0%) Query: 3 MSFKVAFIGAGSIGFTRGLLRDLLTVAEFKEIEVSFMDINPHNLQMVNELCQRDIRENGL 62 MSFKVAFIGAGSIGFTRG+LRDLLTV EF++IEV+F DI+ HNL+MV ELCQRDI ENGL Sbjct: 1 MSFKVAFIGAGSIGFTRGILRDLLTVPEFRDIEVAFTDISRHNLEMVTELCQRDINENGL 60 Query: 63 KIVISATTDRKEALKDAKYVFCTIRMGGLEAFATDVDIPLKYGVDQCVGDTLCAGGIMYG 122 I I +TTDR++AL+DAKYV CTIR+GGLEAF TDVDIPLKYG+DQCVGDTLCAGGIMYG Sbjct: 61 SIAIQSTTDRRQALQDAKYVICTIRVGGLEAFRTDVDIPLKYGIDQCVGDTLCAGGIMYG 120 Query: 123 QRGIAEMLEICRDIREAAAPDVLLLNYSNPMAMLTWACNKYGGVRTIGLCHGVQHGHQQI 182 QRGI EML ICRDIRE APDVLLLNYSNPMAMLTWACN+YGGVRT+GLCHGVQHGH QI Sbjct: 121 QRGIPEMLNICRDIREVCAPDVLLLNYSNPMAMLTWACNRYGGVRTVGLCHGVQHGHHQI 180 Query: 183 AEVYGLEKSEVDIICAGINHQTWYISAKHKGRDLTGGLLEAFEKHPEFSRTEKVRIDMLR 242 AEVYGL+K +VDI+CAGINHQTWYI A H+G+DLT GLLEAFE+HPE+SRTEKVRIDMLR Sbjct: 181 AEVYGLKKQDVDIVCAGINHQTWYIQASHQGKDLTAGLLEAFERHPEYSRTEKVRIDMLR 240 Query: 243 RFGYYSTESNGHLSEYVPWYRKRSGEIMDWIDLGSWINGETGGYLRVCTEGRNWFETDFP 302 RFGYYSTESNGHLSEYVPWYRKR EI +WIDL SWINGETGGYLRVCTEGRNWFETDFP Sbjct: 241 RFGYYSTESNGHLSEYVPWYRKRPEEITEWIDLSSWINGETGGYLRVCTEGRNWFETDFP 300 Query: 303 NWMKDPALEYTSEHRGEEHGSYIIEGLETGRVYRGHFNTVNNGIITNLPDDAVIEAPGYV 362 NWMKDPA+E+ EHRGEEHGSYIIEGLETGRVYRGHFN VN GII NLP+DAVIEAPGYV Sbjct: 301 NWMKDPAMEFKPEHRGEEHGSYIIEGLETGRVYRGHFNVVNEGIIANLPEDAVIEAPGYV 360 Query: 363 DHNGISMPVVGDLPLGPAAVCNVSISVQRLAVEAAVHGDDKLLRQAFMMDPLVGAVCNPK 422 D NGISMP+VG+LPLGPAAVC+VSISVQRLAVEAAVHGDDKLLRQAFMMDPLVGAVCNPK Sbjct: 361 DRNGISMPLVGNLPLGPAAVCSVSISVQRLAVEAAVHGDDKLLRQAFMMDPLVGAVCNPK 420 Query: 423 EIWQMVDEMLVAGEAWLPQYSAAIAEAKVRLASGDLVPTLADNAGAARLKVKSVDEMMQD 482 EIWQ+VDEMLVAGEAWLPQYSA+IAEAK RLA+G+L+PT + GAARLKVK+V+EM D Sbjct: 421 EIWQLVDEMLVAGEAWLPQYSASIAEAKERLAAGNLIPTRS-YEGAARLKVKTVEEMQLD 479 Query: 483 REAANKNAGESDKGKDREKV 502 RE ANKNAGESDKGKDREKV Sbjct: 480 REKANKNAGESDKGKDREKV 499 >B1DD65.1 B1DD65_9BACL Glycoside hydrolase family 4 Length = 502 Score = 851 bits (2199), Expect = 0.0, Method: Compositional matrix adjust. Identities = 401/497 (80%), Positives = 438/497 (88%) Query: 3 MSFKVAFIGAGSIGFTRGLLRDLLTVAEFKEIEVSFMDINPHNLQMVNELCQRDIRENGL 62 MSFKVAF+GAGSIGFTRGL+RDLL V EFK+I+V+F DIN HNL MV +L QRDI ENGL Sbjct: 1 MSFKVAFVGAGSIGFTRGLMRDLLAVPEFKDIQVAFTDINEHNLDMVTQLVQRDINENGL 60 Query: 63 KIVISATTDRKEALKDAKYVFCTIRMGGLEAFATDVDIPLKYGVDQCVGDTLCAGGIMYG 122 I I ATT+R+EALKDA+YVFC +R+GGLEAF D+DIPLKYG+DQCVGDTLCAGGIMYG Sbjct: 61 SIEIQATTNRREALKDARYVFCVVRIGGLEAFQYDIDIPLKYGIDQCVGDTLCAGGIMYG 120 Query: 123 QRGIAEMLEICRDIREAAAPDVLLLNYSNPMAMLTWACNKYGGVRTIGLCHGVQHGHQQI 182 QRGIAEML IC+DIRE A P+ LLLNY+NPMAMLTWACNKYGGVRTIGLCHGVQ GH QI Sbjct: 121 QRGIAEMLNICKDIREVAEPNALLLNYANPMAMLTWACNKYGGVRTIGLCHGVQGGHHQI 180 Query: 183 AEVYGLEKSEVDIICAGINHQTWYISAKHKGRDLTGGLLEAFEKHPEFSRTEKVRIDMLR 242 A G EK +VDIICAGINHQTWYIS K G D TG LLEAFE PEF++TEKVRIDMLR Sbjct: 181 ARALGREKKDVDIICAGINHQTWYISVKTDGVDRTGDLLEAFESVPEFAQTEKVRIDMLR 240 Query: 243 RFGYYSTESNGHLSEYVPWYRKRSGEIMDWIDLGSWINGETGGYLRVCTEGRNWFETDFP 302 RFGYYSTESNGHLSEYVPWYRKR+ EI DWIDLG WINGETGGYLRVCTEGRNWFETDFP Sbjct: 241 RFGYYSTESNGHLSEYVPWYRKRADEIKDWIDLGVWINGETGGYLRVCTEGRNWFETDFP 300 Query: 303 NWMKDPALEYTSEHRGEEHGSYIIEGLETGRVYRGHFNTVNNGIITNLPDDAVIEAPGYV 362 NWMK +YT+E+RGEEHGSYIIEGLETGRVYRGHFN VNNG+ITNLPDDA+IEAPGYV Sbjct: 301 NWMKAEPFKYTAENRGEEHGSYIIEGLETGRVYRGHFNVVNNGVITNLPDDAIIEAPGYV 360 Query: 363 DHNGISMPVVGDLPLGPAAVCNVSISVQRLAVEAAVHGDDKLLRQAFMMDPLVGAVCNPK 422 D NGI+MPVVGDLPLGPAAVCNVSISVQR+AVEAAVHGDDKLLRQA MMDPL GAVCNPK Sbjct: 361 DRNGINMPVVGDLPLGPAAVCNVSISVQRMAVEAAVHGDDKLLRQAMMMDPLTGAVCNPK 420 Query: 423 EIWQMVDEMLVAGEAWLPQYSAAIAEAKVRLASGDLVPTLADNAGAARLKVKSVDEMMQD 482 EIWQMVDE+LVA WLPQY+ AIA A+ RLASG+L+PT + GAARLK KSV+E+ QD Sbjct: 421 EIWQMVDELLVAQAKWLPQYTEAIAAAEERLASGNLLPTRENYQGAARLKTKSVEELSQD 480 Query: 483 REAANKNAGESDKGKDR 499 REAA +NA ESDK K+R Sbjct: 481 REAATRNAIESDKAKER 497 >Q5WL01.1 Q5WL01_BACSK Alpha-galactosidase EC=3.2.1.22 Length = 508 Score = 771 bits (1990), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/497 (72%), Positives = 408/497 (82%), Gaps = 1/497 (0%) Query: 3 MSFKVAFIGAGSIGFTRGLLRDLLTVAEFKEIEVSFMDINPHNLQMVNELCQRDIRENGL 62 M FKVAFIGAGSIGFTR LLRDLL+V F IEV+F DIN NL MV +LCQRDI ENGL Sbjct: 1 MPFKVAFIGAGSIGFTRMLLRDLLSVPAFSTIEVAFTDINQENLNMVTQLCQRDINENGL 60 Query: 63 KIVISATTDRKEALKDAKYVFCTIRMGGLEAFATDVDIPLKYGVDQCVGDTLCAGGIMYG 122 I I AT DR+EAL A+Y+F +R+GGLEAF D+DIPL+YG+DQCVGDTL AGGIMYG Sbjct: 61 PIQIHATLDRREALTGARYIFNVVRVGGLEAFEQDIDIPLRYGIDQCVGDTLSAGGIMYG 120 Query: 123 QRGIAEMLEICRDIREAAAPDVLLLNYSNPMAMLTWACNKYGGVRTIGLCHGVQHGHQQI 182 QRGI EML IC DIR+ A D LLLNY+NPMAMLTWACN YGGV T+GLCHGVQ GH+QI Sbjct: 121 QRGINEMLAICEDIRDVAEQDCLLLNYANPMAMLTWACNHYGGVNTVGLCHGVQGGHRQI 180 Query: 183 AEVYGLEKSEVDIICAGINHQTWYISAKHKGRDLTGGLLEAFEKHPEFSRTEKVRIDMLR 242 A+V+GL+ EVDI+CAGINHQTWYI KH G DLTG LLEAFE HPE+S+TEKVRIDML+ Sbjct: 181 ADVFGLQPEEVDIVCAGINHQTWYIQIKHDGEDLTGKLLEAFENHPEYSKTEKVRIDMLK 240 Query: 243 RFGYYSTESNGHLSEYVPWYRKRSGEIMDWIDLGSWINGETGGYLRVCTEGRNWFETDFP 302 RFGYYSTESNGHLSEYVPWYRKR EI DWIDLG+WINGETGGYLRVC EGRNWF+ DFP Sbjct: 241 RFGYYSTESNGHLSEYVPWYRKRQEEIGDWIDLGTWINGETGGYLRVCKEGRNWFKEDFP 300 Query: 303 NWMKDPALEYTSEHRGEEHGSYIIEGLETGRVYRGHFNTVNNGIITNLPDDAVIEAPGYV 362 NWM +PA + RG+EHGSYI+E LETGR+YRGHFN VN +I+NLP D +IEAPGYV Sbjct: 301 NWMNEPAFVFAESERGQEHGSYIVEALETGRIYRGHFNVVNQNVISNLPSDCIIEAPGYV 360 Query: 363 DHNGISMPVVGDLPLGPAAVCNVSISVQRLAVEAAVHGDDKLLRQAFMMDPLVGAVCNPK 422 D NGI+MPV+GDLP+G A+VCNVSI+VQRLAVEAAV GDD+LLRQAFMMDPL GAVC P Sbjct: 361 DRNGINMPVIGDLPIGCASVCNVSINVQRLAVEAAVAGDDQLLRQAFMMDPLTGAVCTPP 420 Query: 423 EIWQMVDEMLVAGEAWLPQYSAAIAEAKVRLASGDLVPTLADNAGAARLKVKSVDEMMQD 482 EIWQ+VD+ML+A E WLPQY +IAEAK R + + T D +GAARLK K+V EM ++ Sbjct: 421 EIWQLVDDMLIAQEKWLPQYKTSIAEAKQRQQTQQRLAT-KDTSGAARLKTKTVVEMKEN 479 Query: 483 REAANKNAGESDKGKDR 499 R A KNA ++DK R Sbjct: 480 RAEAMKNASQADKAIKR 496 >B8I9A4.1 B8I9A4_CLOCE Glycoside hydrolase family 4 Length = 489 Score = 686 bits (1769), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/488 (66%), Positives = 380/488 (77%) Query: 3 MSFKVAFIGAGSIGFTRGLLRDLLTVAEFKEIEVSFMDINPHNLQMVNELCQRDIRENGL 62 MSFKVAFIGAGS+ FTR L D+++V EF++I+++F DIN NLQ V ELCQRD+ NG+ Sbjct: 1 MSFKVAFIGAGSLVFTRTLFTDIMSVPEFRDIKIAFTDINGDNLQKVAELCQRDLEANGI 60 Query: 63 KIVISATTDRKEALKDAKYVFCTIRMGGLEAFATDVDIPLKYGVDQCVGDTLCAGGIMYG 122 I ATTDR+EA KDAKY+ +R+GGLEAF TD+DIPLKYGVDQCVGDTLC GGIMYG Sbjct: 61 TTKIQATTDRREAFKDAKYIVNCVRIGGLEAFETDIDIPLKYGVDQCVGDTLCTGGIMYG 120 Query: 123 QRGIAEMLEICRDIREAAAPDVLLLNYSNPMAMLTWACNKYGGVRTIGLCHGVQHGHQQI 182 QR IA ML+ C+DIRE +AP +LLNYSNP AM TWACNKYGGVRTIGLCHG HG QI Sbjct: 121 QRVIAAMLDFCKDIREVSAPGAILLNYSNPNAMATWACNKYGGVRTIGLCHGEIHGEDQI 180 Query: 183 AEVYGLEKSEVDIICAGINHQTWYISAKHKGRDLTGGLLEAFEKHPEFSRTEKVRIDMLR 242 A+V G+ ++E+DIICAGINHQTWYIS KHKG++L +L FE HP+FS EKVRID+L+ Sbjct: 181 AQVLGIPRNELDIICAGINHQTWYISVKHKGKELLDKILPGFEAHPKFSEEEKVRIDVLK 240 Query: 243 RFGYYSTESNGHLSEYVPWYRKRSGEIMDWIDLGSWINGETGGYLRVCTEGRNWFETDFP 302 RFGYYSTESNGHLSEYV WYRKR EIM WI+L SWINGETGGYLR+ E RNWFETD+P Sbjct: 241 RFGYYSTESNGHLSEYVAWYRKRPQEIMKWINLDSWINGETGGYLRITREERNWFETDYP 300 Query: 303 NWMKDPALEYTSEHRGEEHGSYIIEGLETGRVYRGHFNTVNNGIITNLPDDAVIEAPGYV 362 + +P +Y RG EH SYIIE LETGR YRGHFN +N G ITNLP ++V+E P YV Sbjct: 301 KILAEPPKKYDGSSRGREHCSYIIESLETGRKYRGHFNVMNEGCITNLPYESVVEVPCYV 360 Query: 363 DHNGISMPVVGDLPLGPAAVCNVSISVQRLAVEAAVHGDDKLLRQAFMMDPLVGAVCNPK 422 D NGIS+P VGDLPLG AAVC+ SI VQRLAVEAAV G+ LL+QA +MDPL GAVCNP Sbjct: 361 DGNGISVPKVGDLPLGCAAVCSQSIWVQRLAVEAAVSGNVTLLKQAALMDPLTGAVCNPP 420 Query: 423 EIWQMVDEMLVAGEAWLPQYSAAIAEAKVRLASGDLVPTLADNAGAARLKVKSVDEMMQD 482 EIWQM+DEML+A E WLPQY I AK R A G+L+P GA R +VK+ E+ + Sbjct: 421 EIWQMIDEMLIAQEKWLPQYVEGIKAAKERFAKGNLIPINEGYRGAVRQRVKTPSEVAAE 480 Query: 483 REAANKNA 490 RE+ + A Sbjct: 481 RESRSITA 488 >Q2CK17.1 Q2CK17_9RHOB Putative uncharacterized protein Length = 498 Score = 614 bits (1584), Expect = e-174, Method: Compositional matrix adjust. Identities = 293/492 (59%), Positives = 368/492 (74%), Gaps = 6/492 (1%) Query: 3 MSFKVAFIGAGSIGFTRGLLRDLLTVAEFKEIEVSFMDINPHNLQMVNELCQRDIRENGL 62 MSFK+ IGAGS+GFT+ L DLL V EF+EIE++ DIN HNL M+ ++ +R + NGL Sbjct: 1 MSFKICIIGAGSVGFTKKLCSDLLKVREFEEIEIALTDINEHNLDMIRQIIERIVSVNGL 60 Query: 63 KIVISATTDRKEALKDAKYVFCTIRMGGLEAFATDVDIPLKYGVDQCVGDTLCAGGIMYG 122 K ++ATTDR+ AL A+Y+ +R+GGLEAF TD+ IPLKYGVDQCVGDT+CAGGIMYG Sbjct: 61 KTRVTATTDRRAALTGARYIMNCVRIGGLEAFETDISIPLKYGVDQCVGDTICAGGIMYG 120 Query: 123 QRGIAEMLEICRDIREAAAPDVLLLNYSNPMAMLTWACNKYGGVRTIGLCHGVQHGHQQI 182 QRGI+ MLE CRDI E A P LLLNY+NPM M+TWA GV T+GLCHGV +GH+QI Sbjct: 121 QRGISAMLEFCRDINEVAEPGALLLNYANPMVMMTWAALG-AGVNTVGLCHGVPNGHRQI 179 Query: 183 AEVYGLEKSEVDIICAGINHQTWYISAKHKGRDL-TGGLLEAFEKHPEFSRTEKVRIDML 241 A G+ EVDI+C+GINHQTWY+ +H GR + L EAFE+HP +S EKVRID+L Sbjct: 180 ARALGVPFEEVDIVCSGINHQTWYLDIRHNGRKIGREELTEAFERHPVYSEQEKVRIDVL 239 Query: 242 RRFGYYSTESNGHLSEYVPWYRKRSGEIMDWIDLGSWINGETGGYLRVCTEGRNWFETDF 301 +RFG+YSTESNGHLSEY+PWYRKR EI +WI+L WINGETGGYLR CTE RNWFE DF Sbjct: 240 KRFGFYSTESNGHLSEYLPWYRKRPEEITNWINLDVWINGETGGYLRYCTENRNWFEEDF 299 Query: 302 PNWMKDPALEYTSEHRGEEHGSYIIEGLETGRVYRGHFNTVNNGIITNLPDDAVIEAPGY 361 P ++ + + R +EH S+I+E LETGRVYRGHFN N GII NLP+D ++E+PG+ Sbjct: 300 PKFLDEAGKSLDAHERTDEHASWILEALETGRVYRGHFNVRNGGIIPNLPEDCIVESPGF 359 Query: 362 VDHNGISMPVVGDLPLGPAAVCNVSISVQRLAVEAAVHGDDKLLRQAFMMDPLVGAVCNP 421 VD GI+M LP A CN S++VQR+AVEAA+ GD +LL+QA + DPLVGA+C P Sbjct: 360 VDRFGINMVEGITLPPACQATCNASVNVQRMAVEAALTGDVELLKQAVLHDPLVGAICTP 419 Query: 422 KEIWQMVDEMLVAGEAWLPQYSAAIAEAKVRLASGDLVPTLADNAGAARLKVKSVDEMMQ 481 E+WQMVDEMLVA E WLPQY+ AI A+ RL + + D AGAARL V+SV+E+ + Sbjct: 420 DEVWQMVDEMLVAQEQWLPQYADAIPAARERLKTPKV--QTRDWAGAARLGVRSVEELRE 477 Query: 482 DREA--ANKNAG 491 +++A A K++G Sbjct: 478 NKKAQEATKDSG 489 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,165,219,289 Number of extensions: 138958402 Number of successful extensions: 336666 Number of sequences better than 1.0e-05: 846 Number of HSP's gapped: 334158 Number of HSP's successfully gapped: 1063 Length of query: 503 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 363 Effective length of database: 1,741,754,103 Effective search space: 632256739389 Effective search space used: 632256739389 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)