BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0020.p (671 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KA88.1 B3KA88_9BACI Histidine kinase HAMP region domain protein 488 e-135 B1DIU9.1 B1DIU9_9BACL Methyl-accepting chemotaxis sensory transd... 293 3e-77 B3KFL9.1 B3KFL9_9BACI Histidine kinase HAMP region domain protein 290 2e-76 Q6VUH1.1 Q6VUH1_9BACL Methyl-accepting chemotaxis protein 156 5e-36 A8VSM6.1 A8VSM6_9BACI Putative methyl-accepting chemotaxis senso... 132 1e-28 >B3KA88.1 B3KA88_9BACI Histidine kinase HAMP region domain protein Length = 678 Score = 488 bits (1255), Expect = e-135, Method: Compositional matrix adjust. Identities = 274/670 (40%), Positives = 411/670 (61%), Gaps = 6/670 (0%) Query: 1 MKLFVILFCTIVLLSSVLGLTSYYAAKGIITDXXXXXXXXXXXXXXDKLDFLFAEYEALS 60 MKLFVI F TIVLLSS LGL SY +K + D DKLDFL A+YEA S Sbjct: 14 MKLFVIFFTTIVLLSSALGLLSYSISKDTVMDQVSEATSGQMKQAADKLDFLLAQYEAAS 73 Query: 61 RQFAVDSILKADMETVSRPGAATVAKVEAEDRIRRKLDSVKGSDERLLGIRLVSKSLVDA 120 RQ+A+D++L+ D+ TVS K +AE+RIR KL+ + SD+RL G+RLV+ +L Sbjct: 74 RQWALDTVLREDLVTVSTADIGIRDKTQAENRIREKLNGMASSDKRLQGMRLVAPNLQAQ 133 Query: 121 ESYKSTGISSVRSAEGIQARVKQLDAAKGNPVWFPVRAKGFFDMYDQSSLTMGRLLRNMQ 180 SY STG S + S++G++A++ ++ A G PVWFP KGF + + +++TMGRLL+N++ Sbjct: 134 SSYNSTGYSGMSSSDGVKAKIDRIIQADGEPVWFPTEIKGFMENENTATMTMGRLLKNLK 193 Query: 181 HPEAEYYMLIEIKGQALTDILSNLHIGLTGEIRILDPDGNIVYGADNKLLGEPSYIHPAA 240 +P A+Y +L+++K +A+ ++L+NL IG G++RI+ D IV+ D L PS I + Sbjct: 194 NPNAQYVLLMDVKDEAVGEVLANLKIGNNGQMRIVAEDNRIVHDMDPAQLMLPSVITVPS 253 Query: 241 EPAEGQQQSFTASNEQGTSQLVVYQPLDTAKWTLMGYAPVSDFTKSADRLLYITXXXXXX 300 + EG +TA Q+VVY PL TA W ++G+APVSDF ++ D+LL +T Sbjct: 254 DAEEGGIY-YTAD-----EQMVVYTPLKTAPWHMVGFAPVSDFVQATDKLLAVTLAVIVA 307 Query: 301 XXXXXXXXXXXXXRLIGRPLGKLAGLMEEGEQGNLQVRTHFKGQDEIGRLGHSFNKMMEQ 360 R +GRPL ++ LMEEGEQGNL+VRT F+ DEIGRLG SFN+MM Q Sbjct: 308 AVLVAVLIGYYMMRTVGRPLNQMCSLMEEGEQGNLRVRTEFRRADEIGRLGQSFNRMMGQ 367 Query: 361 ISLLAGQSNRSAAQVLATSEQLVNASSITSTHAREVXXXXXXXXXXXXXXXXXXXXXNRN 420 IS L Q+N SA +VL T+E+L AS TS A E+ N+ Sbjct: 368 ISALVDQTNASAKEVLVTAEELAEASRGTSLTAGEIAAAMEQIAQGSGSLAMEAERENQL 427 Query: 421 VERMNLQITEVKEINSAMDASAEKVITVSDQGAELMKTLVAQSESTVQMMELIQENSMKL 480 E + +++++V + N + A+A++V+ V+ +GA+ M +LV +S Q+ I +N+ KL Sbjct: 428 AENIGVRMSKVSDTNQVIAAAADRVLRVTGEGAQHMDSLVEKSTRINQVNRAIVQNAEKL 487 Query: 481 RESAHLIRSILTPMIAVNKQTNILALNASIEXXXXXXXXXXXXXXXNEIRGLADESNQSI 540 +++ I IL M + QTN+L++NA+IE NE+R LAD S +SI Sbjct: 488 KDNTASIHKILELMSEITHQTNVLSINATIEAARAGAAGSGFMVVANEVRSLADRSKESI 547 Query: 541 AMVSKITEEISSRIENTVQVVGDTAPLFREQISSVRESSSIFEGVRAEMEQFIGYLNESS 600 V + +I ++ +V + +PLF EQ+ SV+E+ +F+ VR EM+ + ++ES+ Sbjct: 548 QTVGAMILDIRHEVQGSVDALTSASPLFDEQLHSVQEAQGVFDNVRHEMDALLTEISEST 607 Query: 601 ASITELMEYQHQLGESMASVHAVVQQTSASTEEVASMSSQQFTVSEELVALSGKLEKLAE 660 ++ L++ Q L +S++ ++VQ+TSA+TEEVASMSS+QF VSE+LV LS +LE+L+E Sbjct: 608 HAVEVLIDSQLALTDSISEASSIVQETSAATEEVASMSSEQFKVSEKLVGLSSRLEELSE 667 Query: 661 ELKQSLVSFH 670 L++SLV F Sbjct: 668 GLRKSLVMFR 677 >B1DIU9.1 B1DIU9_9BACL Methyl-accepting chemotaxis sensory transducer Length = 718 Score = 293 bits (751), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 215/672 (31%), Positives = 336/672 (50%), Gaps = 10/672 (1%) Query: 2 KLFVILFCTIVLLSSVLGLTSYYAAKGIITDXXXXXXXXXXXXXXDKLDFLFAEYEALSR 61 KLF+I+F +I+ V+G +Y K I+ D LD + YE LS Sbjct: 52 KLFLIIFVSIIACVLVVGQMAYSKTKTIVEDKVSSASFETIVQASANLDIILKTYEDLSM 111 Query: 62 QFAVDSILKADMETVSRPGAATVAKVEAEDRIRRKLDSVKGSDERLLGIRLVSKSLVDAE 121 Q +D + T+ A EA+ + +L + S++ ++G L+ L D Sbjct: 112 QIMIDKEFHKLVSTLEDSTEA-FDLFEAQKDLGERLQNAVMSNKAVIGAYLLP--LDDKL 168 Query: 122 SYKSTGISSVRSAEGIQAR--VKQLDAAKGNPVWFPVRAKGFFDMYDQSSLTMGRLLRNM 179 + G +SV A + ++ +KG W + GF S+ + R + N Sbjct: 169 PTITYGTASVAVAPDLMKSDWFEKTKTSKGGVNWIETQPTGFGTKDKLPSIGLSRQMNNT 228 Query: 180 QHPEAEYYMLIEIKGQALTDILSNLHIGLTGEIRILDPDGNIVYGADNKLLGEPSYIHPA 239 A Y +LIE+ + + + ++ +G +I ++D D + DN L+G+ + + Sbjct: 229 TTNAASYILLIELNLEDIQEKYEDISLGEGSKIYLVDSDNKYISAPDNSLIGKGNVVKLN 288 Query: 240 AEPAEGQQQSFTASNEQGTSQLVVYQPLDTAKWTLMGYAPVSDFTKSADRLLYITXXXXX 299 + ++ S + +G L V++ LDTA W L+G PV++ K A + +T Sbjct: 289 ID----EEGSLKEKSTEGEEVLSVFKTLDTAGWRLVGTIPVNELVKDAGVINKMTWLIAL 344 Query: 300 XXXXXXXXXXXXXXRLIGRPLGKLAGLMEEGEQGNLQVRTHF-KGQDEIGRLGHSFNKMM 358 R I PL + LM EGE+GNL VR++ K +DEIG+LG SFNKMM Sbjct: 345 VAMFIAIAIGMLVIRTIAMPLMAMRNLMNEGERGNLTVRSNITKRKDEIGQLGESFNKMM 404 Query: 359 EQISLLAGQSNRSAAQVLATSEQLVNASSITSTHAREVXXXXXXXXXXXXXXXXXXXXXN 418 QI+ LA Q+ +SA +VL+T+ +L + S T+ ARE+ + Sbjct: 405 IQITALAKQTTQSADEVLSTAGELTDVSKKTAISAREISVATEEIASGASSLAVEAERGS 464 Query: 419 RNVERMNLQITEVKEINSAMDASAEKVITVSDQGAELMKTLVAQSESTVQMMELIQENSM 478 +N Q+ V + M SA +V S QG M L+ ++ T +M + E Sbjct: 465 DLTGNINHQMKSVMDATGHMVESAGEVEKASQQGTAYMGLLIQKTGMTEEMTRSMVEKVD 524 Query: 479 KLRESAHLIRSILTPMIAVNKQTNILALNASIEXXXXXXXXXXXXXXXNEIRGLADESNQ 538 L+ S I IL + + KQTNIL+LNA+IE +EIR LAD+S Q Sbjct: 525 SLKLSTGSIVKILDVLNNLTKQTNILSLNATIEAARAGAAGKGFMVVADEIRKLADQSRQ 584 Query: 539 SIAMVSKITEEISSRIENTVQVVGDTAPLFREQISSVRESSSIFEGVRAEMEQFIGYLNE 598 SI +V +ITE I ++IE TV V+ D PLF+EQI SV+E++ +F V+ +M QF+ L+ Sbjct: 585 SIDVVGQITETIMTQIEETVTVLSDAYPLFQEQIGSVKEANQLFLTVQNQMGQFVQKLDS 644 Query: 599 SSASITELMEYQHQLGESMASVHAVVQQTSASTEEVASMSSQQFTVSEELVALSGKLEKL 658 + SIT L + Q L E+M +V AV QQ+SA++EEVAS+S++Q ++S LV LS KL+ + Sbjct: 645 VTNSITMLDQSQAVLTEAMGNVSAVAQQSSATSEEVASLSTEQLSISNGLVNLSEKLDAV 704 Query: 659 AEELKQSLVSFH 670 + LK+SL F Sbjct: 705 SRGLKESLSRFK 716 >B3KFL9.1 B3KFL9_9BACI Histidine kinase HAMP region domain protein Length = 724 Score = 290 bits (743), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 213/672 (31%), Positives = 335/672 (49%), Gaps = 10/672 (1%) Query: 1 MKLFVILFCTIVLLSSVLGLTSYYAAKGIITDXXXXXXXXXXXXXXDKLDFLFAEYEALS 60 ++LF+I IV VLG SY AK I +K+D + +YE Sbjct: 60 IRLFLIFLSAIVFFVIVLGYLSYNKAKDTIEKNAANGNQQTIIQTSEKIDIILDKYENAG 119 Query: 61 RQFAVDSILKADMETVSRPGAATVAKVEAEDRIRRKLDSVKGSDERLLGIRLVSKSLVDA 120 RQ D L+ + + + E IR KL + SD + I L+ Sbjct: 120 RQIFYDIELQKLLSEYTDKKLSEYDAFTVERTIRDKLSNQITSDSAIRAIYLIPSK---G 176 Query: 121 ESYKSTGISSVRSAEGI-QARVKQLDAAKGNPVWFPVRAKGFFDMYDQSSLTMGRLLRNM 179 E S G SS S + Q+ +L +W P + T+ R L +M Sbjct: 177 EKIISAGQSSESSKTVLEQSWYAELKEGDKKIMWLPTMQNSGAPYF-----TIARSLGSM 231 Query: 180 QHPEAEYYMLIEIKGQALTDILSNLHIGLTGEIRILDPDGNIVYGADNKLLG-EPSYIHP 238 + Y ++ E+ L L +++G G+++++ P+G +V +D+ ++G E ++ Sbjct: 232 NDLTSTYVVMFELNASVLDAQLKGINLGENGQLQLISPEGIVVSSSDSSIVGSETAWTFA 291 Query: 239 AAEPAEGQQQSFTASNEQGTSQLVVYQPLDTAKWTLMGYAPVSDFTKSADRLLYITXXXX 298 PA+ + +E+G + L VY L T W L+G S+ TK A +L IT Sbjct: 292 KEFPADTKTDDVRTKDEEGRNVLAVYNTLSTNDWKLVGSVQTSELTKDASSILVITLIVA 351 Query: 299 XXXXXXXXXXXXXXXRLIGRPLGKLAGLMEEGEQGNLQVRTHFKGQDEIGRLGHSFNKMM 358 R+I RP+GKL LM+EG +GNL VRT K QDEIG+L SFN MM Sbjct: 352 AIDVIFAILIGIWMVRMIARPIGKLNLLMKEGAKGNLNVRTDHKSQDEIGQLSASFNDMM 411 Query: 359 EQISLLAGQSNRSAAQVLATSEQLVNASSITSTHAREVXXXXXXXXXXXXXXXXXXXXXN 418 EQI+ L Q++ +A +VL T+ +L +AS T+ A+E+ + Sbjct: 412 EQITGLVQQTSNTAQEVLRTAAELSDASKKTAISAKEIAVATEEIANGASSLAVEAERGS 471 Query: 419 RNVERMNLQITEVKEINSAMDASAEKVITVSDQGAELMKTLVAQSESTVQMMELIQENSM 478 ++ Q+ V N M SA V + S+ G + + L+ ++ T M + Sbjct: 472 DLTNNISKQMQIVVTANEEMGKSARHVESSSELGTKHLSDLLDKTHQTEDMTRSMMHKVD 531 Query: 479 KLRESAHLIRSILTPMIAVNKQTNILALNASIEXXXXXXXXXXXXXXXNEIRGLADESNQ 538 L+E+ + +L + + KQTNIL+LNA+IE +EIR LAD+S Q Sbjct: 532 GLKETTSSVAKVLDVLQNITKQTNILSLNATIEAARAGAAGKGFMVVADEIRQLADQSRQ 591 Query: 539 SIAMVSKITEEISSRIENTVQVVGDTAPLFREQISSVRESSSIFEGVRAEMEQFIGYLNE 598 SI MVS+IT+ I + + TVQV+ D PLF+ Q+ +V+E++ IF V+ +M +FI L+ Sbjct: 592 SIDMVSQITDRIMAEMNETVQVLLDANPLFKGQMDAVKETNDIFVSVQQQMVEFIDRLDS 651 Query: 599 SSASITELMEYQHQLGESMASVHAVVQQTSASTEEVASMSSQQFTVSEELVALSGKLEKL 658 + SI EL E Q+ L E+M++V AV +++SA++EEVAS+S++Q +VS +LV LS +LE + Sbjct: 652 VTHSIDELNESQNVLSEAMSNVSAVAEESSATSEEVASLSTEQQSVSSQLVNLSTQLENV 711 Query: 659 AEELKQSLVSFH 670 + ELK++L F Sbjct: 712 SNELKETLSRFR 723 >Q6VUH1.1 Q6VUH1_9BACL Methyl-accepting chemotaxis protein Length = 610 Score = 156 bits (395), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 154/629 (24%), Positives = 278/629 (44%), Gaps = 34/629 (5%) Query: 53 FAEYEALSRQFAVDSILKA--DMETVSRPGAATVAKVEAEDRIRRKLDSVKGSDERLLGI 110 ++YE L+ Q +D +++ +METV K + ++ KL +KG + L+GI Sbjct: 1 MSQYEMLTMQVLLDKDVQSALNMETVK------TGKTDQINKTNNKLKELKGPYKDLIGI 54 Query: 111 RLVSKSLVDAESYKSTGISSVRSAEGIQARVKQLDAAKGNPVWFPVRAKGFFDMY--DQS 168 +++ D E + + S + + + Q+ A G V+ + D + Q Sbjct: 55 YMIAD---DLERQRKLPLFYNISDDVRERLIGQVGTA-GGTVYLGLENSIASDEFLNKQK 110 Query: 169 SLTMGRLLRNMQHPEAEYYMLIEIKGQALTDILSNLHIGLTGEIRILDPDGNIVYGADNK 228 GR L+ + + ++L+E+K A+ L++L + G I DG ++ + Sbjct: 111 VFAFGRPLKRLSDNKTVGFLLLEVKADAIKQTLADLRLDDEGNSYIASADGKVLVAEEES 170 Query: 229 LLGEPSYIHPAAEPAEGQQQSFTASNEQGTSQLVVYQPLDTAKWTLMGYAPVSDFTKSAD 288 LLG E Q G +V A W ++ PV + Sbjct: 171 LLGTKLSFGLKENEQENSWQEVI----NGNKFIVTRAASAIAGWQVVSVYPVDKLLEDMR 226 Query: 289 RLLYITXXXXXXXXXXXXXXXXXXXRLIGRPLGKLAGLMEEGEQGNLQVRTHFKGQDEIG 348 ++ R P+ KL +M GE G+L VR G+DE+ Sbjct: 227 QIRSEVIFIALVCSAIIIVLAYFSARRFTHPIEKLRMMMSHGEAGDLTVRADLAGKDELA 286 Query: 349 RLGHSFNKMMEQISLLAGQSNRSAAQVLATSEQLVNASSITSTHAREVXXXXXXXXXXXX 408 LG SFN+M+ +I Q+ +A +VL TS L +S S +A+E+ Sbjct: 287 ALGGSFNQMIGKIRKQFIQTFEAADEVLGTSTHLSESSGKMSKYAKEIANATESIAKGAE 346 Query: 409 XXXXXXXXXN-------RNVERMNLQITEVKEI-NSAMDASAEKVITVSDQGAELMKTLV 460 N NV++M + ++ E+ N A + S QG + M ++ Sbjct: 347 QQATALEKGNGLVLEMTSNVKQMTEDVQDLIEVSNQAQERSV--------QGTQHMTLMI 398 Query: 461 AQSESTVQMMELIQENSMKLRESAHLIRSILTPMIAVNKQTNILALNASIEXXXXXXXXX 520 ++ T Q+M+ I + KL + I SIL + ++KQTNIL+LNA+IE Sbjct: 399 GKNRDTAQIMKEISSHVSKLFDRLGEIDSILELIRNISKQTNILSLNATIEANRAGNAGK 458 Query: 521 XXXXXXNEIRGLADESNQSIAMVSKITEEISSRIENTVQVVGDTAPLFREQISSVRESSS 580 +E+R LA +S S+ + + + I ++ T + + P+ EQI SV +++ Sbjct: 459 GFMVIASEVRALAMQSGDSVTRIVQTLDNIRKDMDATKVALLKSGPIMEEQIHSVEQANL 518 Query: 581 IFEGVRAEMEQFIGYLNESSASITELMEYQHQLGESMASVHAVVQQTSASTEEVASMSSQ 640 IF V+ M++ L+ + ++ + + Q Q+ E ++ AV +Q+ A +++VAS ++ Sbjct: 519 IFNQVQEGMDEANKRLSTFTMTMERMDQSQQQIMEFISDAAAVAEQSMAMSQQVASSAAD 578 Query: 641 QFTVSEELVALSGKLEKLAEELKQSLVSF 669 Q ++E+ V S +LE+++E L+ SL F Sbjct: 579 QQQLTEQFVRWSVQLEQVSERLRVSLNHF 607 >A8VSM6.1 A8VSM6_9BACI Putative methyl-accepting chemotaxis sensory transducer Length = 725 Score = 132 bits (332), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 95/354 (26%), Positives = 174/354 (49%) Query: 316 IGRPLGKLAGLMEEGEQGNLQVRTHFKGQDEIGRLGHSFNKMMEQISLLAGQSNRSAAQV 375 + RP+ + LM++ E G+L VRT+ G++E+G+LG SFN+M++ S + ++ Sbjct: 308 VTRPISNMIRLMKQAEDGDLTVRTNETGKNEMGQLGTSFNRMLQNFSHIIAENKSVTTVA 367 Query: 376 LATSEQLVNASSITSTHAREVXXXXXXXXXXXXXXXXXXXXXNRNVERMNLQITEVKEIN 435 +EQ+ + +S A ++ ++ +E ++ ++T + E Sbjct: 368 KENTEQMKRIADQSSHTADQIASSIQEVANGAMEQVNYADRTSQEMEELSSEMTRMGEAV 427 Query: 436 SAMDASAEKVITVSDQGAELMKTLVAQSESTVQMMELIQENSMKLRESAHLIRSILTPMI 495 + A T S M L A++E Q + I+ + KL + IRS++ + Sbjct: 428 GQVTKVANNTKTSSTNSLGYMNELTAKNEDVGQNIGQIKTSIEKLSQDVEGIRSVVEIID 487 Query: 496 AVNKQTNILALNASIEXXXXXXXXXXXXXXXNEIRGLADESNQSIAMVSKITEEISSRIE 555 ++ QTN+LALNASIE +E+R LA++S QS + I I + + Sbjct: 488 GISDQTNLLALNASIEAARAGEAGKGFAVVADEVRKLAEQSKQSTGKIDGIVSAILGQTK 547 Query: 556 NTVQVVGDTAPLFREQISSVRESSSIFEGVRAEMEQFIGYLNESSASITELMEYQHQLGE 615 ++V++V ++ LF EQ SV E+ + F + + E + SI + E + Q+ Sbjct: 548 DSVKLVTESVALFGEQAKSVEETKASFGDIMTDTEDIFAAVESMERSIETMSEKREQVER 607 Query: 616 SMASVHAVVQQTSASTEEVASMSSQQFTVSEELVALSGKLEKLAEELKQSLVSF 669 ++ + + + TSASTEEV++ + +QF +EEL LS LE +L++ + F Sbjct: 608 AIGDMAEITETTSASTEEVSATTQEQFAAAEELGKLSDILESTMTDLEEVVNRF 661 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.128 0.337 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,852,047,722 Number of extensions: 97489152 Number of successful extensions: 322833 Number of sequences better than 1.0e-05: 7155 Number of HSP's gapped: 309974 Number of HSP's successfully gapped: 7173 Length of query: 671 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 528 Effective length of database: 1,713,491,058 Effective search space: 904723278624 Effective search space used: 904723278624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 135 (56.6 bits)