BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0019.p (282 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KA87.1 B3KA87_9BACI Binding-protein-dependent transport system... 424 e-117 B1DAP9.1 B1DAP9_9BACL Binding-protein-dependent transport system... 354 6e-96 B0K7T5.1 B0K7T5_THEP3 Binding-protein-dependent transport system... 263 2e-68 B8CYJ7.1 B8CYJ7_HALOH Binding-protein-dependent transport system... 262 3e-68 C4L3T0.1 C4L3T0_EXISA Binding-protein-dependent transport system... 260 9e-68 >B3KA87.1 B3KA87_9BACI Binding-protein-dependent transport systems inner membrane component Length = 280 Score = 424 bits (1090), Expect = e-117, Method: Compositional matrix adjust. Identities = 203/280 (72%), Positives = 240/280 (85%) Query: 3 IGRKLANFFRLGFSYXXXXXXXXXXXYPALWILLSSFRPGKSLYSKTLIPEHFTLDHYKE 62 +G K+ N RL SY YPALWI+L+SFRPGKSLYSK+LIPE FTL HYKE Sbjct: 1 MGIKMRNAIRLTASYITLVIIAIGCIYPALWIVLASFRPGKSLYSKSLIPESFTLSHYKE 60 Query: 63 LFTSPVYMFGTWYANTLKIAVFSMLIGVVLTLLTSYALSRFRFKTRKTTMSVLLILGMFP 122 LFTS ++FGTWY NTLKIAVFSM+IG +L LLTSYA+SRFRFK R+ +SV+L+LGMFP Sbjct: 61 LFTSQSFLFGTWYMNTLKIAVFSMIIGTILVLLTSYAVSRFRFKGRQNALSVILVLGMFP 120 Query: 123 GFMSMIAIYLLLKEFNLLDTHLALIIVYAAGAPLGGTLIAKGFLDTVPRSLDEAARIDGA 182 GFMSMIA+Y+LL +LL+TH A+IIVYAAGAPLGGTLI KGFLDT+PRSLDEAA+IDGA Sbjct: 121 GFMSMIALYILLNSLDLLNTHAAMIIVYAAGAPLGGTLIMKGFLDTIPRSLDEAAKIDGA 180 Query: 183 SNFGIFARIILPLSRPMITYMALTQFVGPWVDFIFAKLILRTKDNWTVAVGMWDMVNTMQ 242 +NF IF +IILPLS+PMITYM LT FVGPWVDFIFA+L+LRTK+NWT+AVG+WD+VN+ Q Sbjct: 181 TNFQIFRKIILPLSKPMITYMGLTLFVGPWVDFIFARLVLRTKENWTLAVGLWDLVNSTQ 240 Query: 243 NSNFTLFAAGAVLISVPIMILFAFLQRLLVDGLTAGASKG 282 +SNFTLFAA +VLI++PI +LF FLQRLLVDG+TAGASKG Sbjct: 241 DSNFTLFAAASVLIALPITLLFVFLQRLLVDGMTAGASKG 280 >B1DAP9.1 B1DAP9_9BACL Binding-protein-dependent transport systems inner membrane component Length = 282 Score = 354 bits (908), Expect = 6e-96, Method: Compositional matrix adjust. Identities = 172/282 (60%), Positives = 218/282 (77%) Query: 1 MIIGRKLANFFRLGFSYXXXXXXXXXXXYPALWILLSSFRPGKSLYSKTLIPEHFTLDHY 60 M G+ + RL SY YPALW+LLSSFRPG SLYS + IP+ FTL HY Sbjct: 1 MKAGQSKNRYIRLTLSYVVLTLLAICCLYPALWVLLSSFRPGTSLYSDSFIPKSFTLSHY 60 Query: 61 KELFTSPVYMFGTWYANTLKIAVFSMLIGVVLTLLTSYALSRFRFKTRKTTMSVLLILGM 120 ELF S V+++G WY NTLKIA SM+ +L+ ++ YALSRFRFK RKT ++ LL+LGM Sbjct: 61 TELFHSKVFLYGRWYLNTLKIAFLSMIFSTILSTMSMYALSRFRFKGRKTLLTTLLVLGM 120 Query: 121 FPGFMSMIAIYLLLKEFNLLDTHLALIIVYAAGAPLGGTLIAKGFLDTVPRSLDEAARID 180 FPGFMS++ +Y+ L + NLLDTH A+IIVY+AG+ L + KGF DT+PRSLDE+ARID Sbjct: 121 FPGFMSLVPVYIFLLQLNLLDTHAAIIIVYSAGSVLMNGFVIKGFFDTIPRSLDESARID 180 Query: 181 GASNFGIFARIILPLSRPMITYMALTQFVGPWVDFIFAKLILRTKDNWTVAVGMWDMVNT 240 GA++ IF RI+LPL+RP++ Y+ALT F G WVDFIFA+L+LR+K+NWT+AVGMWD+V++ Sbjct: 181 GANHLQIFYRIMLPLARPILIYVALTTFAGAWVDFIFARLVLRSKENWTLAVGMWDLVSS 240 Query: 241 MQNSNFTLFAAGAVLISVPIMILFAFLQRLLVDGLTAGASKG 282 QNSNFT+FAAGAVLI+VPI +LF FLQ+ LV GLTAGASKG Sbjct: 241 YQNSNFTMFAAGAVLIAVPITLLFVFLQKFLVQGLTAGASKG 282 >B0K7T5.1 B0K7T5_THEP3 Binding-protein-dependent transport systems inner membrane component Length = 296 Score = 263 bits (671), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 133/245 (54%), Positives = 181/245 (73%), Gaps = 2/245 (0%) Query: 37 SSFRPGKSLYSKTLIPEHFTLDHYKELFTSPVYMFGTWYANTLKIAVFSMLIGVVLTLLT 96 SS PG +L+S TLIP++ +L HYK L T+ F WY NTLKIA +M++ V L+ T Sbjct: 53 SSLNPGDTLFSSTLIPKNPSLVHYKTLLTNSD--FPLWYKNTLKIATANMIVSVFLSTST 110 Query: 97 SYALSRFRFKTRKTTMSVLLILGMFPGFMSMIAIYLLLKEFNLLDTHLALIIVYAAGAPL 156 +Y SR+RF RK+ + +L+L MFP F++M AIY+LL + LLDTHL LI+VYA G Sbjct: 111 AYVFSRYRFMGRKSMLMTILVLQMFPSFLAMTAIYVLLLQLGLLDTHLGLILVYATGQIP 170 Query: 157 GGTLIAKGFLDTVPRSLDEAARIDGASNFGIFARIILPLSRPMITYMALTQFVGPWVDFI 216 T +AKG+ D +PRS+DEAA+IDGASNF IF +IILPL++P+IT++ALT F GPW+DF+ Sbjct: 171 YNTWVAKGYFDGIPRSMDEAAKIDGASNFTIFYKIILPLAKPIITFIALTNFTGPWMDFV 230 Query: 217 FAKLILRTKDNWTVAVGMWDMVNTMQNSNFTLFAAGAVLISVPIMILFAFLQRLLVDGLT 276 KLILR+ D T+A+G++DM N+ FTLFAAGAVL+++PI +LF +LQ+ +V+GLT Sbjct: 231 LPKLILRSADKKTLAMGLYDMAVHQHNTKFTLFAAGAVLVAIPITLLFVYLQKHIVEGLT 290 Query: 277 AGASK 281 GA K Sbjct: 291 KGAVK 295 >B8CYJ7.1 B8CYJ7_HALOH Binding-protein-dependent transport systems inner membrane component Length = 280 Score = 262 bits (669), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 137/282 (48%), Positives = 188/282 (66%), Gaps = 2/282 (0%) Query: 1 MIIGRKLANFFRLGFSYXXXXXXXXXXXYPALWILLSSFRPGKSLYSKTLIPEHFTLDHY 60 MI K N +L F+Y +PALWI S PG SL+S IPE+ T HY Sbjct: 1 MIDSYKTRNTIKLTFTYLFIGIMILIALFPALWIFSGSLNPGHSLFSTKFIPENATFIHY 60 Query: 61 KELFTSPVYMFGTWYANTLKIAVFSMLIGVVLTLLTSYALSRFRFKTRKTTMSVLLILGM 120 KELF FG WY NTLK+ + ++GV L LT+YA SR +F RK M V+LI+ + Sbjct: 61 KELFQETD--FGIWYINTLKVGAGTAILGVFLASLTAYAFSRLKFAGRKVGMMVMLIIQV 118 Query: 121 FPGFMSMIAIYLLLKEFNLLDTHLALIIVYAAGAPLGGTLIAKGFLDTVPRSLDEAARID 180 FPG MSM+A+Y+LL LLDTH+ LII+YAAG + + KG+LDT+P+SL+EAA ID Sbjct: 119 FPGVMSMVALYVLLNLVELLDTHIGLIIIYAAGTVPYHSWLMKGYLDTIPKSLEEAAIID 178 Query: 181 GASNFGIFARIILPLSRPMITYMALTQFVGPWVDFIFAKLILRTKDNWTVAVGMWDMVNT 240 GAS++ IF IILPL+ PM++ +AL FV P+ DF+FA+L++ + D WT+A+G+++ V Sbjct: 179 GASHWKIFWTIILPLAIPMLSVLALFYFVAPFGDFLFARLVITSPDKWTLALGLYEFVVD 238 Query: 241 MQNSNFTLFAAGAVLISVPIMILFAFLQRLLVDGLTAGASKG 282 NFT+FAAG++L ++PI+IL +Q +LV GLT GA+KG Sbjct: 239 QYGKNFTIFAAGSLLAAIPILILHLSMQSMLVSGLTRGANKG 280 >C4L3T0.1 C4L3T0_EXISA Binding-protein-dependent transport systems inner membrane component Length = 277 Score = 260 bits (665), Expect = 9e-68, Method: Compositional matrix adjust. Identities = 132/271 (48%), Positives = 185/271 (68%), Gaps = 1/271 (0%) Query: 12 RLGFSYXXXXXXXXXXXYPALWILLSSFRPGKSLYSKTLIPEHFTLDHYKELFTSPVYMF 71 L SY YP LW++ +SF PG+++ S T+IP + T HYK LF + + Sbjct: 8 NLAISYVILTLASIIILYPMLWVVGTSFNPGRTITS-TIIPSNPTFIHYKNLFDLTISDY 66 Query: 72 GTWYANTLKIAVFSMLIGVVLTLLTSYALSRFRFKTRKTTMSVLLILGMFPGFMSMIAIY 131 WY NTLKIA +M + VVL +T Y SR+RF RK ++ + L+L M P F+++IAIY Sbjct: 67 SLWYVNTLKIAFITMALSVVLVTITGYVFSRYRFVGRKNSLVLFLVLQMVPQFVAIIAIY 126 Query: 132 LLLKEFNLLDTHLALIIVYAAGAPLGGTLIAKGFLDTVPRSLDEAARIDGASNFGIFARI 191 +LL NLLDTH ALI++YA GA T +AKG+ DT+P+ LDEAAR+DGA + IF +I Sbjct: 127 VLLNMLNLLDTHTALILLYAGGAIPMNTYLAKGYFDTIPKELDEAARMDGAGHLRIFWQI 186 Query: 192 ILPLSRPMITYMALTQFVGPWVDFIFAKLILRTKDNWTVAVGMWDMVNTMQNSNFTLFAA 251 ILPL++P++ +AL F+GP+ DFI A L+LR+ + T+AVG+++M++ ++NFTLFAA Sbjct: 187 ILPLAKPIVATIALFNFMGPFNDFILASLVLRSPEKQTLAVGLYNMISKQFDNNFTLFAA 246 Query: 252 GAVLISVPIMILFAFLQRLLVDGLTAGASKG 282 GAVL ++PI+ILF QR V GLTAG +KG Sbjct: 247 GAVLSALPIVILFFMFQRYFVSGLTAGGTKG 277 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.143 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,475,954,049 Number of extensions: 56442904 Number of successful extensions: 314559 Number of sequences better than 1.0e-05: 6197 Number of HSP's gapped: 306382 Number of HSP's successfully gapped: 6198 Length of query: 282 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 148 Effective length of database: 1,798,280,193 Effective search space: 266145468564 Effective search space used: 266145468564 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)