BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0018.p (422 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KA86.1 B3KA86_9BACI Binding-protein-dependent transport system... 597 e-169 B1DAP8.1 B1DAP8_9BACL Binding-protein-dependent transport system... 533 e-149 C5D6S7.1 C5D6S7_9BACI Binding-protein-dependent transport system... 449 e-124 B0K7T4.1 B0K7T4_THEP3 Binding-protein-dependent transport system... 400 e-109 C2IPP5.1 C2IPP5_VIBCH Maltose/maltodextrin ABC transporter perme... 359 3e-97 >B3KA86.1 B3KA86_9BACI Binding-protein-dependent transport systems inner membrane component Length = 437 Score = 597 bits (1540), Expect = e-169, Method: Compositional matrix adjust. Identities = 295/422 (69%), Positives = 353/422 (83%) Query: 1 MGLGQIYNRQFIKGLIFIAVEAGALVYFIANLGEAFWGITTLGDSPSHLEKVKGIAKMVP 60 MGLGQ+YNRQ+IKG+I + V +L YFI NLG+A WG+ TLG+ SHLEKV G+ +MV Sbjct: 16 MGLGQLYNRQWIKGVILLIVGVFSLYYFINNLGDAIWGMVTLGEQGSHLEKVNGLTQMVA 75 Query: 61 GDHSIVILIQSLITLMFFVLFLIAWYMNIRDAHKTGEERETGRPSNTFKQSVRYILDYKF 120 GDHSI +LI+ LITL+ F LFLI +Y NI++A+ G+ RE G N F+Q++ YI ++KF Sbjct: 76 GDHSINLLIEGLITLIMFALFLIGYYANIKNAYNAGKLREKGVTPNNFRQTLYYIFEWKF 135 Query: 121 AQSXXXXXXXXXXXXXXXXXXXXXXLAFTNYAAPDHIPPAKLVDWVGFETFRNLLVLKSW 180 AQS LAFTNY+AP+++PPA LVDWVGF+TF NL+ LKSW Sbjct: 136 AQSFLTLPAIGILFFTVMPIIFMVLLAFTNYSAPNNLPPASLVDWVGFKTFTNLVALKSW 195 Query: 181 SHTFYGVLTWTIIWAVLSTITTYFGGMLVALLINQKGIRFKGLWRLILIVPYAIPQMISL 240 SHTF+GVLTWTIIWAVL+T+TTYFGG+LVALLI QKGIRFKG+WR+ILI+PYAIPQ+ISL Sbjct: 196 SHTFFGVLTWTIIWAVLATVTTYFGGVLVALLIQQKGIRFKGVWRVILIIPYAIPQLISL 255 Query: 241 LLMRNLFNGQFGPINQYLGYFGLGGLPWLTDPFWAKVTVIMVNMWVGIPVSMLLIMGVLT 300 L+MRN+FNGQFGPINQYL YFGL GLPWLTDP WAKVTVI+VNMWVGIPVSMLL+M VLT Sbjct: 256 LVMRNMFNGQFGPINQYLRYFGLEGLPWLTDPVWAKVTVIVVNMWVGIPVSMLLVMSVLT 315 Query: 301 TIPRDMYEAAEVDGATNYQKFRIVTLPMILFSTAPTLIMQFAGNINNFNAIYLLTQGNPV 360 TIP+D+YEAA+VDGAT +QKF+I+TLPMILFSTAP LI QFAGNINNFN I+LLT GNPV Sbjct: 316 TIPKDLYEAADVDGATGFQKFKIITLPMILFSTAPVLITQFAGNINNFNLIFLLTNGNPV 375 Query: 361 NGNYQYAGSTDLLVTWLYKLTLDQNKNNMASAIGIILFIIVAGFSLYNYRRTKSFQEEDM 420 G+YQYAG+TDLLVTWLYKLTLDQ + +MASAIGII+F+I+A FS+YNYRRT+SF+EEDM Sbjct: 376 VGDYQYAGATDLLVTWLYKLTLDQQRYSMASAIGIIIFMIIATFSIYNYRRTRSFKEEDM 435 Query: 421 IQ 422 IQ Sbjct: 436 IQ 437 >B1DAP8.1 B1DAP8_9BACL Binding-protein-dependent transport systems inner membrane component Length = 435 Score = 533 bits (1373), Expect = e-149, Method: Compositional matrix adjust. Identities = 277/422 (65%), Positives = 331/422 (78%) Query: 1 MGLGQIYNRQFIKGLIFIAVEAGALVYFIANLGEAFWGITTLGDSPSHLEKVKGIAKMVP 60 MGLGQ+YNRQ++KG++ + EA ALVYFI L WGI TLGD+PS + KV + + V Sbjct: 14 MGLGQLYNRQYMKGILLLLTEAAALVYFIPRLVHDLWGIWTLGDTPSKMVKVGKVYQTVK 73 Query: 61 GDHSIVILIQSLITLMFFVLFLIAWYMNIRDAHKTGEERETGRPSNTFKQSVRYILDYKF 120 GDHSI ++I LIT++ LF+ + MNIRDA++TG R+ G + TF++SVRY+ D KF Sbjct: 74 GDHSIFLMINGLITVLVLALFITIYIMNIRDAYRTGANRDQGIQAATFRESVRYVWDRKF 133 Query: 121 AQSXXXXXXXXXXXXXXXXXXXXXXLAFTNYAAPDHIPPAKLVDWVGFETFRNLLVLKSW 180 L+FTNY+ P HIPPAKLVDWVGF TF++LL LKSW Sbjct: 134 PHLLLTIPGLGVLFLTIMPIVFMILLSFTNYSLPQHIPPAKLVDWVGFNTFKDLLTLKSW 193 Query: 181 SHTFYGVLTWTIIWAVLSTITTYFGGMLVALLINQKGIRFKGLWRLILIVPYAIPQMISL 240 S TF+GV TWTIIWAVL+T T YFGG+LVALLI QK ++FK LWR + I+PYAIPQ+ISL Sbjct: 194 SRTFFGVFTWTIIWAVLATATCYFGGLLVALLIEQKDVKFKKLWRTLFILPYAIPQLISL 253 Query: 241 LLMRNLFNGQFGPINQYLGYFGLGGLPWLTDPFWAKVTVIMVNMWVGIPVSMLLIMGVLT 300 L+MRNLFNGQFGPINQYL YFG G LPWLTDP WAK T I+VNMWVGIPVSM+LI+GVLT Sbjct: 254 LVMRNLFNGQFGPINQYLSYFGFGKLPWLTDPTWAKATAILVNMWVGIPVSMVLILGVLT 313 Query: 301 TIPRDMYEAAEVDGATNYQKFRIVTLPMILFSTAPTLIMQFAGNINNFNAIYLLTQGNPV 360 IP+D+YEAA+VDGA+ YQKFRIVTLP +LF+TAP LI QFAGNINNFN I+LLT G P+ Sbjct: 314 AIPKDLYEAADVDGASGYQKFRIVTLPFVLFATAPILITQFAGNINNFNLIFLLTNGEPL 373 Query: 361 NGNYQYAGSTDLLVTWLYKLTLDQNKNNMASAIGIILFIIVAGFSLYNYRRTKSFQEEDM 420 NGNYQYAGSTDLLVTWLYKLTL+ ++ NMASAIGII+FII+A FS+ NYRRTKSF+EEDM Sbjct: 374 NGNYQYAGSTDLLVTWLYKLTLNNSQFNMASAIGIIIFIIIATFSIINYRRTKSFKEEDM 433 Query: 421 IQ 422 IQ Sbjct: 434 IQ 435 >C5D6S7.1 C5D6S7_9BACI Binding-protein-dependent transport systems inner membrane component Length = 447 Score = 449 bits (1154), Expect = e-124, Method: Compositional matrix adjust. Identities = 208/421 (49%), Positives = 299/421 (71%), Gaps = 3/421 (0%) Query: 1 MGLGQIYNRQFIKGLIFIAVEAGALVYFIANLGEAFWGITTLGDSPSHLEKVKGIAKMVP 60 MGLGQIYNRQ+IKG+++ VE +V+ + WG+ TLG+ + + + + Sbjct: 30 MGLGQIYNRQYIKGILYALVEIYVIVFLTNPIVNGLWGLITLGEQAQVRRRGRVV---IE 86 Query: 61 GDHSIVILIQSLITLMFFVLFLIAWYMNIRDAHKTGEERETGRPSNTFKQSVRYILDYKF 120 GDHSI ++I+ LI ++ V+FL+ + NIRDA+KTG+ ETG N+F +S++ +Y + Sbjct: 87 GDHSIFLMIEGLIIVIVLVIFLLFYIYNIRDAYKTGKLHETGEKVNSFIESLKKSWEYGY 146 Query: 121 AQSXXXXXXXXXXXXXXXXXXXXXXLAFTNYAAPDHIPPAKLVDWVGFETFRNLLVLKSW 180 A +AFTN+++P+H+PP LVDWVGF+ F +L L++W Sbjct: 147 AYILLTPAAIFTLFLSVLPLTFGILIAFTNFSSPNHLPPKNLVDWVGFQNFIDLFRLQAW 206 Query: 181 SHTFYGVLTWTIIWAVLSTITTYFGGMLVALLINQKGIRFKGLWRLILIVPYAIPQMISL 240 S TFYG+ TW IIWA+L+T TT+F GM AL+IN +R K +WR I I+P+A+PQ IS+ Sbjct: 207 SKTFYGIATWNIIWAILATFTTFFVGMFFALIINHNKVRLKRMWRTIFILPWAVPQFISI 266 Query: 241 LLMRNLFNGQFGPINQYLGYFGLGGLPWLTDPFWAKVTVIMVNMWVGIPVSMLLIMGVLT 300 L+ RN+FNGQFGP+NQYL G+ +PWL+DPFWAKV ++ +N+W+G P M LI GVLT Sbjct: 267 LVFRNIFNGQFGPLNQYLSTLGIDPIPWLSDPFWAKVALVTINIWLGFPFWMALIAGVLT 326 Query: 301 TIPRDMYEAAEVDGATNYQKFRIVTLPMILFSTAPTLIMQFAGNINNFNAIYLLTQGNPV 360 I +++YEAAEVDGA+ +QKFR +T P++++ T+P LIM FAGNINNFN IYL TQG P Sbjct: 327 NIDKELYEAAEVDGASTFQKFRSITFPLLMYYTSPLLIMSFAGNINNFNVIYLFTQGGPA 386 Query: 361 NGNYQYAGSTDLLVTWLYKLTLDQNKNNMASAIGIILFIIVAGFSLYNYRRTKSFQEEDM 420 +G Y YAGSTDLL++W+YKLTL+ ++ MAS + I++FI+VA FS++N+R+TK+F+EE+M Sbjct: 387 DGKYTYAGSTDLLISWIYKLTLNHSQFYMASVLSILIFIVVAVFSIWNFRQTKAFKEEEM 446 Query: 421 I 421 + Sbjct: 447 M 447 >B0K7T4.1 B0K7T4_THEP3 Binding-protein-dependent transport systems inner membrane component Length = 429 Score = 400 bits (1028), Expect = e-109, Method: Compositional matrix adjust. Identities = 207/422 (49%), Positives = 279/422 (66%), Gaps = 7/422 (1%) Query: 1 MGLGQIYNRQFIKGLIFIAVEAGALVYFIANLGEAFWGITTLGDSPSHLEKVKGIAKMVP 60 MGLGQ+YNR+ KG++F A+ ++ I A+WG+ TLG+ P H V GIA+ Sbjct: 15 MGLGQLYNREIAKGILF-AIVNIIVLINIPYFRYAYWGLITLGEQPLH--YVNGIAE--- 68 Query: 61 GDHSIVILIQSLITLMFFVLFLIAWYMNIRDAHKTGEERETGRPSNTFKQSVRYILDYKF 120 GDHSI +LIQ +I ++ +L ++ + +NI DA KTGE E G + VRY+ F Sbjct: 69 GDHSIFLLIQGIIAVLVTLLIILIYVLNIIDAFKTGEAIEQGEKPMGIVKFVRYVSHKYF 128 Query: 121 AQSXXXXXXXXXXXXXXXXXXXXXXLAFTNYAAPDHIPPAKLVDWVGFETFRNLLVLKSW 180 +AFTNY+AP+H+PP LVDWVG F+NLL LK W Sbjct: 129 PYLSLSPAFVLILFFTLLPILFTISIAFTNYSAPNHLPPRNLVDWVGVTNFKNLLNLKIW 188 Query: 181 SHTFYGVLTWTIIWAVLSTITTYFGGMLVALLINQKGIRFKGLWRLILIVPYAIPQMISL 240 +TF GV WTIIWA+L+++T+YFGG+L+ALL+N ++ K WR I I+PYAIP IS+ Sbjct: 189 KNTFIGVGIWTIIWALLASLTSYFGGLLLALLVNSGDVQLKKFWRTIYILPYAIPGFISI 248 Query: 241 LLMRNLFNGQFGPINQYLGYFGLGGLPWLTDPFWAKVTVIMVNMWVGIPVSMLLIMGVLT 300 L MR +F G GP+N +L G +PWLT+P AKV +I+VN+W+G P M L+ G LT Sbjct: 249 LTMRLMFTG-LGPVNNFLVKLGFERMPWLTNPTMAKVVLILVNIWLGAPYFMALMSGTLT 307 Query: 301 TIPRDMYEAAEVDGATNYQKFRIVTLPMILFSTAPTLIMQFAGNINNFNAIYLLTQGNPV 360 IP D+YEAA++DGAT YQKFR +TLP++L +T P LI+ F N NNF IYLLT G P Sbjct: 308 NIPGDLYEAADIDGATPYQKFRKITLPLVLHATMPLLILSFTHNFNNFTMIYLLTSGGPA 367 Query: 361 NGNYQYAGSTDLLVTWLYKLTLDQNKNNMASAIGIILFIIVAGFSLYNYRRTKSFQEEDM 420 N Y+YAG TD+L++W+YKLTL+QN+ NMA+ I II+FI+VA S + + RTKSF+EED+ Sbjct: 368 NPAYRYAGHTDILISWIYKLTLEQNQYNMAAVISIIMFIVVATISAFTFSRTKSFREEDL 427 Query: 421 IQ 422 IQ Sbjct: 428 IQ 429 >C2IPP5.1 C2IPP5_VIBCH Maltose/maltodextrin ABC transporter permease protein MalF Length = 427 Score = 359 bits (921), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 182/418 (43%), Positives = 268/418 (64%), Gaps = 12/418 (2%) Query: 1 MGLGQIYNRQFIKGLIFIAVEAGALVYFIANLGEAFWGITTLGDSPSHLEKVKGIAKMVP 60 MG Q+ ++ +I G +F A++ ++Y + +L A G+ +LGD + + + K++ Sbjct: 19 MGSTQLAHKHWITGGVFFAIQCLFILY-LPDLATALKGLISLGD----VAQTRQGFKVIQ 73 Query: 61 GDHSIVILIQSLITLMFFVLFLIAWYMNIRDAHKTGEERETGRPSNTFKQSVRYILDYKF 120 GD+SI +L++ +I L+F L +IA+ MN+R A R T R Q ++ I D KF Sbjct: 74 GDNSIFMLVEGVIALLFLFLVIIAYVMNVRHAQACESCRLTVR------QQLKAIYDEKF 127 Query: 121 AQSXXXXXXXXXXXXXXXXXXXXXXLAFTNYAAPDHIPPAKLVDWVGFETFRNLLVLKSW 180 A ++FTNY+AP HIPP LVDWVGF+ F L LK W Sbjct: 128 AFIVLSPAFIASIAFIIMPIAITVLVSFTNYSAPHHIPPKNLVDWVGFKNFLTLFELKIW 187 Query: 181 SHTFYGVLTWTIIWAVLSTITTYFGGMLVALLINQKGIRFKGLWRLILIVPYAIPQMISL 240 S TF GV TWT+IWA+ +TI T G ++AL + + I+ K LWR + I+PYAIP ++L Sbjct: 188 SSTFVGVATWTVIWAIAATICTCGFGFILALALENRNIKAKKLWRFVFILPYAIPAFVTL 247 Query: 241 LLMRNLFNGQFGPINQYLGYFGLGGLPWLTDPFWAKVTVIMVNMWVGIPVSMLLIMGVLT 300 L+ R L NG GP+N L +G + +L+DP+ AK+TVI V++WVG P MLLI G LT Sbjct: 248 LMFRLLLNG-IGPVNATLNAWGFESVAFLSDPYLAKLTVIAVSVWVGAPYFMLLISGALT 306 Query: 301 TIPRDMYEAAEVDGATNYQKFRIVTLPMILFSTAPTLIMQFAGNINNFNAIYLLTQGNPV 360 IPRD+YEA+EVDGA+ +Q+FR +TLPM+L AP+L+M FA N NNF AI+LLT+G P+ Sbjct: 307 NIPRDLYEASEVDGASKFQQFREITLPMVLHQVAPSLVMTFAHNFNNFGAIFLLTEGGPI 366 Query: 361 NGNYQYAGSTDLLVTWLYKLTLDQNKNNMASAIGIILFIIVAGFSLYNYRRTKSFQEE 418 N Y++AG TD+L+TW+YKLTLD + +AS I I++F+ ++ +++ +RR KSF+++ Sbjct: 367 NPEYRFAGHTDILITWIYKLTLDFQQYQIASVISIVIFLFLSVIAIWQFRRMKSFKDD 424 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.142 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,427,097,319 Number of extensions: 98194067 Number of successful extensions: 351530 Number of sequences better than 1.0e-05: 5894 Number of HSP's gapped: 346219 Number of HSP's successfully gapped: 5894 Length of query: 422 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 284 Effective length of database: 1,760,596,133 Effective search space: 500009301772 Effective search space used: 500009301772 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)