BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0017.p (444 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KA85.1 B3KA85_9BACI Extracellular solute-binding protein family 1 549 e-154 B1DAP7.1 B1DAP7_9BACL Extracellular solute-binding protein family 1 462 e-128 B0K7T3.1 B0K7T3_THEP3 Extracellular solute-binding protein, fami... 351 9e-95 C5D6S6.1 C5D6S6_9BACI Extracellular solute-binding protein family 1 347 1e-93 B4BIQ5.1 B4BIQ5_9BACI Extracellular solute-binding protein family 1 264 1e-68 >B3KA85.1 B3KA85_9BACI Extracellular solute-binding protein family 1 Length = 436 Score = 549 bits (1415), Expect = e-154, Method: Compositional matrix adjust. Identities = 261/444 (58%), Positives = 331/444 (74%), Gaps = 8/444 (1%) Query: 1 MELKKLMVLTAAFSMAVSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60 M++KKL+ L A F++A SI Sbjct: 1 MKMKKLLTLAATFTLAFSITACGGNTAAPATEAPAETPPAQETPAENTETQE-------- 52 Query: 61 IKPEEGASLLVWESKEERVFAEEIAKQFTAKYNVPVKIEEVAPPDQVGKLTQDGPSGLAA 120 +KPEEGA L+VWES++ER + + +AK+FT KY V V IEE++P DQV KL QDGPSGLAA Sbjct: 53 LKPEEGAKLVVWESRDERAYTDAVAKEFTEKYGVEVTIEELSPTDQVTKLAQDGPSGLAA 112 Query: 121 DVVLIPHDNLGKAASASLLLPNDIFAEQTKAANTEASIVGSSYDGELYGYPRAAETYALY 180 DVVL PHDNLGKA + +L+LPND + E+TK+ NTEA+I G SYDG LYGYPRAAETY +Y Sbjct: 113 DVVLFPHDNLGKAVATNLVLPNDFYEEKTKSNNTEAAIQGVSYDGVLYGYPRAAETYLMY 172 Query: 181 YNKSLVKEAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFGKDGT 240 YNK LVKE P T ++VI F KTFTDK+KNKYG M+E GN+YF YPF+A+ GGY+FG G Sbjct: 173 YNKDLVKEVPTTMEEVIEFSKTFTDKSKNKYGFMFETGNLYFAYPFLASNGGYIFGNGGA 232 Query: 241 DKDDIGLNNEGAIEGLKTFVKLKEVLPIKSGDINSDIKRSLFSSGDVAMDMTGPWELAAY 300 D +DIGLNNEGA++ ++ + LKE+LP+KSGDI DIKR LF++GD+AMD+ GPWEL Y Sbjct: 233 DVNDIGLNNEGAVKSMEVYASLKEILPVKSGDITPDIKRGLFTAGDLAMDINGPWELGGY 292 Query: 301 KQALGDKLGVAPVPTVDGKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSKESQLELNK 360 K+ALGDKLGVAP+PT+ G+ A++FSGIK + V+++T+YP AA+L+A+FA+SKE+QL+L+ Sbjct: 293 KEALGDKLGVAPIPTIGGQPAVSFSGIKAWYVSSFTKYPQAARLFADFATSKEAQLQLSA 352 Query: 361 LVGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAALPEIWDNN 420 LVGS+PTNNEA +D QI D Y+SAF EQ K+SQPMPS+PEM NVWSPV AAL EIWDNN Sbjct: 353 LVGSVPTNNEAQEDDQIKNDEYVSAFTEQFKSSQPMPSVPEMANVWSPVGAALAEIWDNN 412 Query: 421 ADPKAAMDKAVQQIKDLNNGAAAK 444 D K +DKAV Q+KDLN+G A + Sbjct: 413 KDVKETLDKAVTQVKDLNSGGAGE 436 >B1DAP7.1 B1DAP7_9BACL Extracellular solute-binding protein family 1 Length = 441 Score = 462 bits (1189), Expect = e-128, Method: Compositional matrix adjust. Identities = 221/380 (58%), Positives = 287/380 (75%), Gaps = 1/380 (0%) Query: 61 IKPEEGASLLVWESKEERVFAEEIAKQFTAKYNVPVKIEEVAPPDQVGKLTQDGPSGLAA 120 I PEEGA+L VW+SK ER F EE+AK+FTA+YN+PVKIEEV PDQV KLT DGP+G+ A Sbjct: 60 IVPEEGATLTVWDSKNERSFVEEMAKEFTAQYNIPVKIEEVESPDQVKKLTTDGPAGIGA 119 Query: 121 DVVLIPHDNLGKAASASLLLPNDIFAEQTKAANTEASIVGSSYDGELYGYPRAAETYALY 180 D+V PHDNLG+A +A L+LPND F E TK AN EA++ + DG LYGYPR+ ETYAL+ Sbjct: 120 DIVTFPHDNLGQAVAAGLILPNDFFEEDTKTANAEAAVNAVTSDGILYGYPRSVETYALF 179 Query: 181 YNKSLVKEAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFGKDGT 240 YNK LV+ P +FDD++ F KTF D AKNKY +MWE+GN YF Y F A+TGGY+FG++GT Sbjct: 180 YNKDLVQTPPASFDDIVTFSKTFNDPAKNKYALMWEMGNFYFNYIFFASTGGYVFGENGT 239 Query: 241 DKDDIGLNNEGAIEGLKTFVKLKEVLPIKSGDINSDIKRSLFSSGDVAMDMTGPWELAAY 300 + DIGLNN+GA+EGLK F LK++LPIKSGD ++DI + F+ +AM +TGPW++ + Sbjct: 240 NAADIGLNNDGAVEGLKYFQSLKQILPIKSGDASADIIQGKFADKSLAMTITGPWKVGDF 299 Query: 301 KQALGDKLGVAPVPTVDGKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSKESQLELNK 360 K LG GVAP+PT++GK A++FSG+K + VN++T+YPNA++L+A F SSK QL+ Sbjct: 300 KN-LGINFGVAPIPTINGKPAVSFSGVKAWYVNSFTKYPNASRLFANFISSKAGQLKDYS 358 Query: 361 LVGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAALPEIWDNN 420 L G+IP N EA++DP D S FA Q NSQ MPSIPEMG+VW P+ AAL +IW+ N Sbjct: 359 LTGAIPANKEAMEDPTFKADEISSGFAAQFVNSQAMPSIPEMGSVWDPIGAALADIWNEN 418 Query: 421 ADPKAAMDKAVQQIKDLNNG 440 DPKAA+D AV Q+++ G Sbjct: 419 KDPKAALDNAVTQVQEAAKG 438 >B0K7T3.1 B0K7T3_THEP3 Extracellular solute-binding protein, family 1 Length = 429 Score = 351 bits (900), Expect = 9e-95, Method: Compositional matrix adjust. Identities = 176/378 (46%), Positives = 244/378 (64%), Gaps = 3/378 (0%) Query: 61 IKPEEGASLLVWESK-EERVFAEEIAKQFTAKYNVPVKIEEVAPPDQVGKLTQDGPSGLA 119 +KPE GA LLVWES+ E F + AK+FT KY V V E V D G+L DGP+G+ Sbjct: 48 LKPEPGAKLLVWESEGPEGEFIQYAAKKFTEKYGVEVVYEPVTHTDARGRLATDGPAGIG 107 Query: 120 ADVVLIPHDNLGKAASASLLLPNDIFAEQTKAANTEASIVGSSYDGELYGYPRAAETYAL 179 ADV PHD+ G+ + L+ PNDIFA++ + + ++ SY G +YGYP ETYAL Sbjct: 108 ADVFAAPHDHTGELVVSGLIQPNDIFADRIRNEFMQGAVDAVSYQGVVYGYPTGIETYAL 167 Query: 180 YYNKSLVKEAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFGKDG 239 +YNK VKE PKT++D+I F K F + AKN++ MW+VGN YF + FIA GGY+FG G Sbjct: 168 FYNKDYVKEPPKTYEDLIKFAKEFNNPAKNQFAFMWDVGNAYFSHSFIAGYGGYVFGDGG 227 Query: 240 TDKDDIGLNNEGAIEGLKTFVKLKEVLPIKSGDINSDIKRSLFSSGDVAMDMTGPWELAA 299 T+KDDIGLN++ A+EG K V LKE+LP+K+ D N + +F G AM + GPW + Sbjct: 228 TNKDDIGLNSKEAVEGAKFLVSLKEILPLKTDDTNYQVMDGMFKEGKAAMIINGPWAVRG 287 Query: 300 YKQALGDKLGVAPVPTV-DGKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSKESQLEL 358 Y++A G G+AP+P + + K +FSG++ V+AYT+YP AAKL+A+F +S+E L+ Sbjct: 288 YQEA-GVNFGIAPLPLLPNDKHPASFSGVRTMFVSAYTKYPKAAKLFADFITSEEMLLKR 346 Query: 359 NKLVGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAALPEIWD 418 ++ IP + I DPY + F EQA+ + PMPSIP+MG VW P A +W+ Sbjct: 347 YEITKQIPPMKSLMDADIIKNDPYAAPFLEQAQFAVPMPSIPQMGVVWEPYARAFQVMWN 406 Query: 419 NNADPKAAMDKAVQQIKD 436 + DP+ A+D+AVQ IKD Sbjct: 407 DGVDPQKALDEAVQTIKD 424 >C5D6S6.1 C5D6S6_9BACI Extracellular solute-binding protein family 1 Length = 432 Score = 347 bits (891), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 185/378 (48%), Positives = 249/378 (65%), Gaps = 5/378 (1%) Query: 61 IKPEEGASLLVWESKEE-RVFAEEIAKQFTAKYNVPVKIEEVAPPDQVGKLTQDGPSGLA 119 IKPE+GA L VWES + + +A++FT KY VPV EEV D KL DGP+GLA Sbjct: 49 IKPEKGAKLKVWESGGAIGEWTKYVAEEFTKKYGVPVTFEEVGHTDAPEKLKTDGPAGLA 108 Query: 120 ADVVLIPHDNLGKAASASLLLPNDIFAEQTKAANTEASIVGSSYDGELYGYPRAAETYAL 179 ADV PHD+LG A L+L N F E+ + +++I G++ DG LYGYP + ETYAL Sbjct: 109 ADVFAAPHDHLGSMVPAGLVLEN-FFPEEYQDKFMKSAIEGTTVDGTLYGYPTSIETYAL 167 Query: 180 YYNKSLVKEAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFGKDG 239 +YNK LVK+ PKT D++I K TD NKYG M EV N+YF Y FI GGY+FG + Sbjct: 168 FYNKDLVKKLPKTMDELITQAKELTDIRNNKYGFMMEVANLYFVYSFIGGYGGYVFGDNN 227 Query: 240 TDKDDIGLNNEGAIEGLKTFVKL-KEVLPIKSGDINSDIKRSLFSSGDVAMDMTGPWELA 298 T+ DIGLNNEGA++ K ++ KE+LP+K DI D+K+SLF+ G +A ++ GPW +A Sbjct: 228 TNPKDIGLNNEGAVKAGKLMQRIHKEILPLKVEDITYDVKQSLFNEGKLAFNIDGPWAVA 287 Query: 299 AYKQALGDKLGVAPVPTVD-GKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSKESQLE 357 ++ A G GV P+P ++ G+T +FSGI+ F VNAYT+YPNAA L+A+FA+S+E L+ Sbjct: 288 GHRDA-GVNFGVIPLPKLENGQTPTSFSGIRAFYVNAYTKYPNAASLFAKFATSEEMLLK 346 Query: 358 LNKLVGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAALPEIW 417 ++ G +P L + I D SAF EQAK + PMP+IP+M VW P+ +AL IW Sbjct: 347 RFEMTGQLPPVQSLLDNETIKNDEIASAFLEQAKYAVPMPNIPQMPMVWEPMASALTTIW 406 Query: 418 DNNADPKAAMDKAVQQIK 435 ++ DPK A+D AV QIK Sbjct: 407 NDGKDPKEALDAAVDQIK 424 >B4BIQ5.1 B4BIQ5_9BACI Extracellular solute-binding protein family 1 Length = 424 Score = 264 bits (675), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 146/380 (38%), Positives = 231/380 (60%), Gaps = 12/380 (3%) Query: 62 KPEEGASLLVW--ESKEERVFAEEIAKQFTAKYNVPVKIEEVAPPDQVGKLTQDGPSGLA 119 KPE+ L+VW + ++++ ++I +++T K + +++ V+ DQ KL DGP+G Sbjct: 43 KPEK---LVVWVNDDEKQKQALKDIFQKYTEKTGIKIEMVAVSMLDQTKKLALDGPAGKG 99 Query: 120 ADVVLIPHDNLGKAASASLLLPNDIFAEQTKAANTEASIVGSSYDGELYGYPRAAETYAL 179 DV PHD +G L P D+ K + +I +YDG+ YG P ETY + Sbjct: 100 PDVFYQPHDRIGDIVLQGLADPIDL--GDAKGEYSPTAIEAVTYDGQTYGVPTVVETYGV 157 Query: 180 YYNKSLVKEAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFGKDG 239 +YNK+LV EAPKT DD++ K T+ AK++YG + E N YF YPF A GGY+F + Sbjct: 158 FYNKNLVSEAPKTIDDLMKIAKEKTNAAKDQYGFLMEAENFYFVYPFFAGYGGYVFKNEN 217 Query: 240 --TDKDDIGLNNEGAIEGLKTFVKLKEVLPIKSGDINSDIKRSLFSSGDVAMDMTGPWEL 297 D DIGL NEGA++G + + I ++ +D+ LF+ G+VA+ +TGPW + Sbjct: 218 GKYDTSDIGLANEGAVKGAELVQSWFQNGYIPK-EVTNDVMNGLFTKGNVAVAITGPWNI 276 Query: 298 AAYKQALGDKLGVAPVPTVD-GKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSKESQL 356 A+YK+ALGDKL AP+P ++ G+ +F G+K + ++AY+Q+ A + +F +++E+ L Sbjct: 277 ASYKEALGDKLATAPLPVLENGEHPKSFVGVKTWMLSAYSQHKEWAIDFMKFVTNEENSL 336 Query: 357 ELNKLVGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAALPEI 416 ++ G +P N +AL + +IT DP I+ FAEQ + +PMP++PEM VW P+ AL I Sbjct: 337 HYYEVAGEMPANEKALTNEKITNDPLIAGFAEQIQYGEPMPNVPEMSQVWDPMGNALQFI 396 Query: 417 WDNNADPKAAMDKAVQQIKD 436 + DPKA +++AV+ I+D Sbjct: 397 AKGD-DPKAVLNEAVKTIQD 415 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.131 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,498,184,646 Number of extensions: 108956531 Number of successful extensions: 203869 Number of sequences better than 1.0e-05: 1334 Number of HSP's gapped: 201001 Number of HSP's successfully gapped: 1334 Length of query: 444 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 305 Effective length of database: 1,751,175,118 Effective search space: 534108410990 Effective search space used: 534108410990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)