BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0016.p (581 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4I1.1 B3K4I1_9BACI Alpha amylase catalytic region 868 0.0 Q84AP9.1 Q84AP9_9BACL Cyclodextrinase enzyme 847 0.0 Q59226.1 Q59226_BACSP Cyclomaltodextrinase EC=3.2.1.54 807 0.0 B1D8P8.1 B1D8P8_9BACL Alpha amylase catalytic region 765 0.0 Q08341.1 CDAS_BACSH Cyclomaltodextrinase EC=3.2.1.54 672 0.0 >B3K4I1.1 B3K4I1_9BACI Alpha amylase catalytic region Length = 582 Score = 868 bits (2244), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/580 (70%), Positives = 468/580 (80%), Gaps = 1/580 (0%) Query: 1 MLLEAVYHRPRLNWSYAYDHETIHLRLRAKKGDLTEVFAWAGDKYAWDATKELIPMTLFT 60 M LEA+YHRPR NWSYAY+ TIHLR+R KK DLTEV+A AGDKY WD T E IPMT + Sbjct: 1 MFLEAIYHRPRKNWSYAYNGTTIHLRIRTKKDDLTEVYALAGDKYLWDQTMEYIPMTKLS 60 Query: 61 SDAMFDYWECSSVPPYRRLKYGFLLQKGHERIWMTESEFVTERPANPNRLFEFPYINRGD 120 SD MFDYWEC + PPYRRLKYGFLLQ+G E+ WMTE +F+TE PANP+RLFE+P+IN D Sbjct: 61 SDEMFDYWECEATPPYRRLKYGFLLQQGDEKRWMTEYDFLTEPPANPDRLFEYPFINPDD 120 Query: 121 VFTPPAWVKDAVFYQIFPERFANGDPSRNPADVQPWGET-PTPENFFGGDLQGVIDHLDH 179 VF PPAWVKDA+FYQIFPERFANGD S +P PWG PTP+NFFGGDLQGVIDHLDH Sbjct: 121 VFHPPAWVKDAIFYQIFPERFANGDTSNDPEGALPWGSAEPTPKNFFGGDLQGVIDHLDH 180 Query: 180 LSELGITGIYFTPIFTATTNHKYDTEDYMSVDPHFGDVETLKKLVAACHERGIRVLLDAV 239 LSELG+ IYFTP+FTATTNHKYDTEDYM +DP FGD +TLKKLV CH RGIRVLLDAV Sbjct: 181 LSELGVNAIYFTPLFTATTNHKYDTEDYMEIDPQFGDKDTLKKLVDLCHARGIRVLLDAV 240 Query: 240 FNHSGRTFAPFVDVLEKGEASPFKDWFHVREFPLQVVDNIPTYDAFAFEPLMPKLNTENP 299 FNHSGRTFAPFVDV + G S +KDWF+VR PL+VVD IPTYD FAFEPLMPKLNTENP Sbjct: 241 FNHSGRTFAPFVDVQKNGLNSKYKDWFYVRSLPLEVVDGIPTYDTFAFEPLMPKLNTENP 300 Query: 300 AVKEYLLKVAEYWITEVGIDGWRLDVANEVDFEFWRDFRKVVKRANPDAYILGEIWHESS 359 V+ YLLK AEYWI E GIDGWRLDVANEV FWR+FR+VVK+ANPDAYILGE+WHESS Sbjct: 301 EVRAYLLKAAEYWIKETGIDGWRLDVANEVGHSFWREFRQVVKKANPDAYILGEVWHESS 360 Query: 360 PWLEGDKFDAVMNYPFTDAVLDFFVYGTLDAEGFANAIGKQLSRYPLQASEVAFNLLDSH 419 WLEGD+FDAVMNYPFT+AVLDFF+ T DAE F+ +GKQL+ YP QASEV FNLLDSH Sbjct: 361 IWLEGDQFDAVMNYPFTNAVLDFFINRTADAEKFSFMLGKQLAGYPRQASEVMFNLLDSH 420 Query: 420 DTPRLLTLAGGDKNKQKLAALFQFTFMGTPCIYYGDEIGMDGEGDPGCRKCMEWNPEHQD 479 DT RLLT A GDK KLAA+FQFT+ GTPCIYYGDEIGMDG DPGCRKCMEW+ D Sbjct: 421 DTARLLTQANGDKRLMKLAAMFQFTYFGTPCIYYGDEIGMDGGHDPGCRKCMEWDESKHD 480 Query: 480 LDLFSFYRKLIEIRSSHPELRTGTFTFLEAGPQGSKLAYERRLGDNLILVLVNTEETAQT 539 DLF FY+ LI++R H LRTGTF FL+A G K+AY R + IL+ VN ++ T Sbjct: 481 KDLFHFYQTLIKLRKEHSALRTGTFKFLKAEKNGGKIAYLREDDNETILIAVNNDKAGNT 540 Query: 540 FSLDVEDRNWENLFTGDTLRAERSKLSIKLPAYGYAVLKA 579 +L V DR W NL+ G+ L A++ +L++KLPAYG+A+LKA Sbjct: 541 MTLPVLDRQWTNLWNGEGLSAKQGQLTVKLPAYGFAILKA 580 >Q84AP9.1 Q84AP9_9BACL Cyclodextrinase enzyme Length = 653 Score = 847 bits (2187), Expect = 0.0, Method: Compositional matrix adjust. Identities = 395/580 (68%), Positives = 460/580 (79%), Gaps = 1/580 (0%) Query: 1 MLLEAVYHRPRLNWSYAYDHETIHLRLRAKKGDLTEVFAWAGDKYAWDATKELIPMTLFT 60 M LEAVYHRPR NWSYAY+ T+HLR+R KK D+T V+A AGDKY WD T E +PMT Sbjct: 1 MFLEAVYHRPRKNWSYAYNGTTVHLRIRTKKDDMTAVYALAGDKYMWDHTMEYVPMTKLA 60 Query: 61 SDAMFDYWECSSVPPYRRLKYGFLLQKGHERIWMTESEFVTERPANPNRLFEFPYINRGD 120 +D +FDYWEC PPYRR+KYGFLLQ+GHE+ WMTE +F+TE PANP+RLFE+P+IN D Sbjct: 61 TDELFDYWECEVTPPYRRVKYGFLLQQGHEKRWMTEYDFLTEPPANPDRLFEYPFINPVD 120 Query: 121 VFTPPAWVKDAVFYQIFPERFANGDPSRNPADVQPWGET-PTPENFFGGDLQGVIDHLDH 179 VF PPAWVKDA+FYQIFPERFANGD +P PWG PTP FFGGDLQGVIDHLDH Sbjct: 121 VFQPPAWVKDAIFYQIFPERFANGDTRNDPEGTLPWGSADPTPSCFFGGDLQGVIDHLDH 180 Query: 180 LSELGITGIYFTPIFTATTNHKYDTEDYMSVDPHFGDVETLKKLVAACHERGIRVLLDAV 239 LS+LG+ +YFTP+F ATTNHKYDTEDY +DP FGD +TLKKLV CHERGIRVLLDAV Sbjct: 181 LSKLGVNAVYFTPLFKATTNHKYDTEDYFQIDPQFGDKDTLKKLVDLCHERGIRVLLDAV 240 Query: 240 FNHSGRTFAPFVDVLEKGEASPFKDWFHVREFPLQVVDNIPTYDAFAFEPLMPKLNTENP 299 FNHSGRTF PFVDVL+ GE S +KDWFH+R PL+VVD IPTYD FAFEPLMPKLNTE+P Sbjct: 241 FNHSGRTFPPFVDVLKNGEKSKYKDWFHIRSLPLEVVDGIPTYDTFAFEPLMPKLNTEHP 300 Query: 300 AVKEYLLKVAEYWITEVGIDGWRLDVANEVDFEFWRDFRKVVKRANPDAYILGEIWHESS 359 VKEYLLK AEYWI E GIDGWRLDVANEV +FWR+FR+VVK+ANPDAYILG++WHESS Sbjct: 301 EVKEYLLKAAEYWIRETGIDGWRLDVANEVSHQFWREFRRVVKQANPDAYILGDVWHESS 360 Query: 360 PWLEGDKFDAVMNYPFTDAVLDFFVYGTLDAEGFANAIGKQLSRYPLQASEVAFNLLDSH 419 WLEGD+FDAVMNYPFT+AVLDFF++ DAE F+ +GKQL+ YP QASEV FNLLDSH Sbjct: 361 IWLEGDQFDAVMNYPFTNAVLDFFIHQIADAEKFSFMLGKQLAGYPRQASEVMFNLLDSH 420 Query: 420 DTPRLLTLAGGDKNKQKLAALFQFTFMGTPCIYYGDEIGMDGEGDPGCRKCMEWNPEHQD 479 DT RLLT A GDK K KLA LFQFT+ GTPCIYYGDE+G+DG DPGCRKCMEW+ D Sbjct: 421 DTARLLTQADGDKRKMKLAVLFQFTYFGTPCIYYGDEVGLDGGHDPGCRKCMEWDETKHD 480 Query: 480 LDLFSFYRKLIEIRSSHPELRTGTFTFLEAGPQGSKLAYERRLGDNLILVLVNTEETAQT 539 DLF+FY+ +I +R +H LRTGTF FL A ++AY R + ILV++N ++ T Sbjct: 481 KDLFAFYQTVIRLRQAHAALRTGTFKFLTAEKNSRQIAYLREDDQDTILVVMNNDKAGHT 540 Query: 540 FSLDVEDRNWENLFTGDTLRAERSKLSIKLPAYGYAVLKA 579 +L V W +L+ D L A +L++KLPAYG+AVLKA Sbjct: 541 LTLPVRHAQWTHLWNDDVLTAAHGQLTVKLPAYGFAVLKA 580 >Q59226.1 Q59226_BACSP Cyclomaltodextrinase EC=3.2.1.54 Length = 558 Score = 807 bits (2084), Expect = 0.0, Method: Compositional matrix adjust. Identities = 376/541 (69%), Positives = 433/541 (80%), Gaps = 1/541 (0%) Query: 1 MLLEAVYHRPRLNWSYAYDHETIHLRLRAKKGDLTEVFAWAGDKYAWDATKELIPMTLFT 60 M LEAVYHRPR NWSYAY+ T+HLR+R KK D+T V+A AGDKY WD T E +PMT Sbjct: 1 MFLEAVYHRPRKNWSYAYNGTTVHLRIRTKKDDMTAVYALAGDKYMWDHTMEYVPMTKLA 60 Query: 61 SDAMFDYWECSSVPPYRRLKYGFLLQKGHERIWMTESEFVTERPANPNRLFEFPYINRGD 120 +D +FDYWEC PPYRR+KYGFLLQ+GHE+ WMTE +F+TE P NP+RLFE+P+IN D Sbjct: 61 TDELFDYWECEVTPPYRRVKYGFLLQQGHEKRWMTEYDFLTEPPRNPDRLFEYPFINPVD 120 Query: 121 VFTPPAWVKDAVFYQIFPERFANGDPSRNPADVQPWGET-PTPENFFGGDLQGVIDHLDH 179 VF PPAWVKDA+FYQIFPERFANGD +P PWG PTP FFGGDLQGVIDHLDH Sbjct: 121 VFQPPAWVKDAIFYQIFPERFANGDTRNDPEGTLPWGSADPTPSCFFGGDLQGVIDHLDH 180 Query: 180 LSELGITGIYFTPIFTATTNHKYDTEDYMSVDPHFGDVETLKKLVAACHERGIRVLLDAV 239 LS+LG+ +YFTP+F ATTNHKYDTEDY +DP FGD +TLKKLV CHERGIRVLLDAV Sbjct: 181 LSKLGVNAVYFTPLFKATTNHKYDTEDYFQIDPQFGDKDTLKKLVDLCHERGIRVLLDAV 240 Query: 240 FNHSGRTFAPFVDVLEKGEASPFKDWFHVREFPLQVVDNIPTYDAFAFEPLMPKLNTENP 299 FNHSGRTF PFVDVL+ GE S +KDWFH+R PL+VVD IPTYD FAFEPLMPKLNTE+P Sbjct: 241 FNHSGRTFPPFVDVLKNGEKSKYKDWFHIRSLPLEVVDGIPTYDTFAFEPLMPKLNTEHP 300 Query: 300 AVKEYLLKVAEYWITEVGIDGWRLDVANEVDFEFWRDFRKVVKRANPDAYILGEIWHESS 359 VKEYLLK AEYWI E GIDGWRLDVANEV +FWR+FR+VVK+ANPDAYILGE+WHESS Sbjct: 301 DVKEYLLKAAEYWIRETGIDGWRLDVANEVSHQFWREFRRVVKQANPDAYILGEVWHESS 360 Query: 360 PWLEGDKFDAVMNYPFTDAVLDFFVYGTLDAEGFANAIGKQLSRYPLQASEVAFNLLDSH 419 WLEGD+FDAVMNYPFT+AVLDFF++ DAE F+ +GKQL+ YP QASEV FNLLDSH Sbjct: 361 IWLEGDQFDAVMNYPFTNAVLDFFIHQIADAEKFSFMLGKQLAGYPRQASEVMFNLLDSH 420 Query: 420 DTPRLLTLAGGDKNKQKLAALFQFTFMGTPCIYYGDEIGMDGEGDPGCRKCMEWNPEHQD 479 DT RLLT A GDK K KLA LFQFT+ GTPCIYYGDE+G+DG DPGCRKCMEW+ D Sbjct: 421 DTARLLTQADGDKRKMKLAVLFQFTYFGTPCIYYGDEVGLDGGHDPGCRKCMEWDETKHD 480 Query: 480 LDLFSFYRKLIEIRSSHPELRTGTFTFLEAGPQGSKLAYERRLGDNLILVLVNTEETAQT 539 DLF+FY+ +I +R +H LRTGTF FL A ++AY R + ILV++N ++ T Sbjct: 481 KDLFAFYQTVIRLRQAHAALRTGTFKFLTAEKNSRQIAYLREDDQDTILVVMNNDKAGHT 540 Query: 540 F 540 Sbjct: 541 L 541 >B1D8P8.1 B1D8P8_9BACL Alpha amylase catalytic region Length = 583 Score = 765 bits (1976), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/585 (61%), Positives = 439/585 (75%), Gaps = 6/585 (1%) Query: 1 MLLEAVYHRPRLNWSYAYDHETIHLRLRAKKGDLTEVFAWAGDKYAWDATKELIPMTLFT 60 MLLEA+YHRP+ NW+YAY+H T+H+R+R KK D V AGDKY W T + M + + Sbjct: 1 MLLEAIYHRPKQNWAYAYNHTTLHIRIRTKKSDARSVELIAGDKYNWAGTFTITDMAVLS 60 Query: 61 SDAMFDYWECSSVPPYRRLKYGFLLQKGHERIWMTESEFVTERPANPNRLFEFPYINRGD 120 SDA+FDYWE S PP+RRL+Y F L G E + MTES F E P NPN F+FPYIN D Sbjct: 61 SDALFDYWEASITPPFRRLRYAFRLTDGDETVIMTESGFPAELPDNPNTFFDFPYINPVD 120 Query: 121 VFTPPAWVKDAVFYQIFPERFANGDPSRNPADVQPWGETPTPENFFGGDLQGVIDHLDHL 180 VF+PPAWVKDAVFYQIFPERFANGDPS NP + WG PTP NFFGGDLQGV+DHLD+L Sbjct: 121 VFSPPAWVKDAVFYQIFPERFANGDPSLNPETTEAWGGKPTPTNFFGGDLQGVLDHLDYL 180 Query: 181 SELGITGIYFTPIFTATTNHKYDTEDYMSVDPHFGDVETLKKLVAACHERGIRVLLDAVF 240 +ELGI +YFTP+F ATTNHKYDTE+YM VD HFG E LK+LV CH+RGIR+LLDAVF Sbjct: 181 AELGINAVYFTPLFEATTNHKYDTENYMQVDRHFGTNEKLKELVEECHKRGIRLLLDAVF 240 Query: 241 NHSGRTFAPFVDVLEKGEASPFKDWFHVREFPLQVVDNIPTYDAFAFEPLMPKLNTENPA 300 NH+GRTF PF D+L+ GE S + +WFHVRE+PL++ D IPTY+ FAFEP+MPKLNTENP Sbjct: 241 NHAGRTFPPFADLLKNGEKSVYANWFHVREWPLRIEDGIPTYETFAFEPMMPKLNTENPD 300 Query: 301 VKEYLLKVAEYWITEVGIDGWRLDVANEVDFEFWRDFRKVVKRANPDAYILGEIWHESSP 360 VK YL VA YWI ++GIDGWRLDVANEVD +FWR+FR+ VK PDAYILGEIWH++ Sbjct: 301 VKAYLFNVARYWIEKIGIDGWRLDVANEVDHQFWREFRQAVKSVKPDAYILGEIWHDAMM 360 Query: 361 WLEGDKFDAVMNYPFTDAVLDFFVYGTLDAEGFANAIGKQLSRYPLQASEVAFNLLDSHD 420 WLEGD+FDAVMNYPFT+AVLDFF + T++ + FA+AIG+QL+ YP Q +E AFNLLDSHD Sbjct: 361 WLEGDQFDAVMNYPFTNAVLDFFGHQTINGKTFADAIGRQLAAYPQQVTETAFNLLDSHD 420 Query: 421 TPRLLTLAGGDKNKQKLAALFQFTFMGTPCIYYGDEIGMDGEGDPGCRKCMEWNPEHQDL 480 TPRLLT+ G D + KLAALFQ T+ GTPCIYYGDE+GMDG+GDP CRKCM W PE QD Sbjct: 421 TPRLLTICGDDADSMKLAALFQLTYPGTPCIYYGDEVGMDGDGDPDCRKCMVWEPEQQDR 480 Query: 481 DLFSFYRKLIEIRSSHPELRTGTFTFLEA--GPQGSKLAYERRLGDNLILVLVNTEETAQ 538 +L +FY++ + +RS H LR+ FL+A G G + YERR GD ILV +N Sbjct: 481 ELLAFYKQALALRSKHAALRSADIRFLQAADGEDGGVIVYERREGDERILVAMNARGREA 540 Query: 539 TFSLDVEDRNWENLFTGDTLRAERS--KLSIKLPAYGYAVLKALL 581 ++ +W +L GD R E + KL ++LP YGY + K L+ Sbjct: 541 VAAVP-SGGSWVSLL-GDEQRLEPAAGKLQLRLPGYGYGIWKQLV 583 >Q08341.1 CDAS_BACSH Cyclomaltodextrinase EC=3.2.1.54 Length = 591 Score = 672 bits (1735), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/583 (56%), Positives = 402/583 (68%), Gaps = 4/583 (0%) Query: 1 MLLEAVYHRPRLNWSYAYDHETIHLRLRAKKGDLTEVFAWAGDKYAWDATKELIPMTLFT 60 ++LEAVYHR NWSYAY+ T+H+R+R K+ ++ + G+KY + KE IPM Sbjct: 2 IMLEAVYHRMGQNWSYAYNDSTLHIRIRTKRDNVPRIDLHCGEKYDPEKYKETIPMERMA 61 Query: 61 SDAMFDYWECSSVPPYRRLKYGFLLQKGH-ERIWMTESEFVTERP-ANPNRLFEFPYINR 118 SD +FDYW+ + P YRRL Y F L + + ++ E F + P LF+FPY+NR Sbjct: 62 SDGLFDYWQAAVQPRYRRLVYYFALHSDNGDAVYFMEKGFFDQPPKVMYEGLFDFPYLNR 121 Query: 119 GDVFTPPAWVKDAVFYQIFPERFANGDPSRNPADVQPWGETPTPENFFGGDLQGVIDHLD 178 DV TPPAWVK+A+FYQIFPERFANGDPS +P VQ WG TP+ NFFGGDLQGVIDHLD Sbjct: 122 QDVHTPPAWVKEAIFYQIFPERFANGDPSNDPEGVQEWGGTPSAGNFFGGDLQGVIDHLD 181 Query: 179 HLSELGITGIYFTPIFTATTNHKYDTEDYMSVDPHFGDVETLKKLVAACHERGIRVLLDA 238 +LS+LG+ +YF P+F ATTNHKYDT DYM +DP FG E LK+LV ACH RG+RVLLDA Sbjct: 182 YLSDLGVNALYFNPLFAATTNHKYDTADYMKIDPQFGTNEKLKELVDACHARGMRVLLDA 241 Query: 239 VFNHSGRTFAPFVDVLEKGEASPFKDWFHVREFPLQVVDNIPTYDAFAFEPLMPKLNTEN 298 VFNH G TF PFVDVL G S + DWFHVRE+PL+VVD IPTYD FAFEP+MPKLNT N Sbjct: 242 VFNHCGHTFPPFVDVLNNGLNSRYADWFHVREWPLRVVDGIPTYDTFAFEPIMPKLNTGN 301 Query: 299 PAVKEYLLKVAEYWITEVGIDGWRLDVANEVDFEFWRDFRKVVKRANPDAYILGEIWHES 358 VK YLL V YW+ E+G+DGWRLDVANEVD +FWR+FR +KR NP AYILGEI H+S Sbjct: 302 EEVKAYLLNVGRYWLEEMGLDGWRLDVANEVDHQFWREFRSEIKRINPSAYILGEIMHDS 361 Query: 359 SPWLEGDKFDAVMNYPFTDAVLDFFVYGTLDAEGFANAIGKQLSRYPLQASEVAFNLLDS 418 PWL+GD+FDAVMNYPFT+ +L+FF +A FA AIG QL+ YP Q +EV+FNLL S Sbjct: 362 MPWLQGDQFDAVMNYPFTNILLNFFARRLTNAAEFAQAIGTQLAGYPQQVTEVSFNLLGS 421 Query: 419 HDTPRLLTLAGGDKNKQKLAALFQFTFMGTPCIYYGDEIGMDGEGDPGCRKCMEWNPEHQ 478 HDT RLLTL G+ + KLA LFQ T+ GTPCIYYGDEIGMDGE DP RKCMEW+ Q Sbjct: 422 HDTTRLLTLCSGNVERMKLATLFQLTYQGTPCIYYGDEIGMDGEYDPLNRKCMEWDKSKQ 481 Query: 479 DLDLFSFYRKLIEIRSSHPELRTGTFTFLEAGPQGSKLAYERRLGDNLILVLVNTEETAQ 538 + +L +F+R +I +R +HP LR FL L YER + L+++N E+ Sbjct: 482 NTELLAFFRSMISLRKAHPALRGSGLRFLPVLEHPQLLVYERWDDNERFLIMLNNEDAPV 541 Query: 539 TFSLDVED--RNWENLFTGDTLRAERSKLSIKLPAYGYAVLKA 579 + +W + E S + LP YGYA+L A Sbjct: 542 NVVIPAAQPGASWRTVNGEPCAVVEESSIQAALPPYGYAILHA 584 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.140 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,876,929,710 Number of extensions: 174669540 Number of successful extensions: 361190 Number of sequences better than 1.0e-05: 4659 Number of HSP's gapped: 350114 Number of HSP's successfully gapped: 5150 Length of query: 581 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 440 Effective length of database: 1,732,333,088 Effective search space: 762226558720 Effective search space used: 762226558720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)