BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0014.p (2165 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3P9.1 B3K3P9_9BACI Pullulanase, extracellular 2494 0.0 Q2BFA5.1 Q2BFA5_9BACI Alkaline amylopullulanase 1129 0.0 P70983.1 P70983_BACSP Alkaline amylopullulanase 850 0.0 Q9K6N1.1 Q9K6N1_BACHD Alkaline amylopullulanase 827 0.0 Q93UZ9.1 Q93UZ9_9BACI Alkaline pullulanase 808 0.0 >B3K3P9.1 B3K3P9_9BACI Pullulanase, extracellular Length = 2528 Score = 2494 bits (6464), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1274/2169 (58%), Positives = 1545/2169 (71%), Gaps = 61/2169 (2%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGD--KDGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGEQP 58 VD+P+K AQKI FLV++ + GD KDGGDK F L+DRY L+I++GD+ VY SP+GE P Sbjct: 415 VDIPMKPGAQKINFLVLDPAKGDAGKDGGDKGFTLVDRYQHLFIQEGDNTVYTSPYGEVP 474 Query: 59 VSLVSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTSVEVDT 118 LVSAE+L+ K+ L FT+TDGL+A LK+ + + +K+G PV + I T++EV Sbjct: 475 TGLVSAEVLAQGKLQLGFTMTDGLEADTLKNELQIKNKDGHPVAIQNVRITGNTTIEV-A 533 Query: 119 AAFALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPMAS 178 A+F L++ PLSV Y+GK VSAS GWRM+DEMY Y GDDLGATY A LKLWAP A+ Sbjct: 534 ASFQLDQIPLSVTYAGKTVSASTGWRMLDEMYAYDGDDLGATYQAG--GAELKLWAPTAT 591 Query: 179 SVVANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNG 238 V A VYSK DA Q +G V+L GDKGVWS LKP DL +D RGY+YQYEVTNNG Sbjct: 592 EVTAKVYSKDDATQFIGNVALQKGDKGVWSVRLKPGDL----GIQDFRGYFYQYEVTNNG 647 Query: 239 VTKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKRED 298 VTK VLDPYAKSMA F V++ G GPDGDTVGKAAIVDLS T+P DIPGYE+RED Sbjct: 648 VTKTVLDPYAKSMAQFRVNTKGEVGPDGDTVGKAAIVDLSQTDPAGLDFADIPGYEQRED 707 Query: 299 AVIYEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNGDE 358 A+I+E+HVRDFTSD +IE++L WGSY AF +KL+YIKSLG+TH+QL+PVMAWY GDE Sbjct: 708 AIIWEIHVRDFTSDPTIENDLHNATWGSYDAFKEKLEYIKSLGVTHVQLLPVMAWYYGDE 767 Query: 359 TRMTHRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMG 418 M +RE YS ++NEYNWGYDPHSYFSPDGAYS+ P DPE RIKELK +I+A+HEA MG Sbjct: 768 AAMKNRELDYSAKDNEYNWGYDPHSYFSPDGAYSEDPTDPELRIKELKAMINAIHEADMG 827 Query: 419 VILDVVYTHMAKKDFLDDIVPNYYAFQDAXXXXXXXXXXXLATSHKMAEKLMVDSVKYWF 478 V+LDVVYTHMAK DFL+DIVPNYYAFQDA LAT+HKMAEKLMVDSVKYWF Sbjct: 828 VVLDVVYTHMAKADFLNDIVPNYYAFQDANGNFIGGFGNNLATNHKMAEKLMVDSVKYWF 887 Query: 479 SEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGAD 538 EYKIDGMRWDMMG INPKALFIGEGW TF G SDP+LAGKGAD Sbjct: 888 DEYKIDGMRWDMMGDATYEAVQKAYDAAAAINPKALFIGEGWVTFGGHLSDPSLAGKGAD 947 Query: 539 QAWMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPADDP 598 Q WMDKTD VGVFSDEFRNELKSG+G+EGEPRFITGGAR I IFNNI A+PSN DDP Sbjct: 948 QNWMDKTDDVGVFSDEFRNELKSGFGNEGEPRFITGGARDIQTIFNNIKAQPSNTKEDDP 1007 Query: 599 GDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQAGQ 658 GD+V YIEAHDNL L+D+IAQ+IKKDPSI AN+LEI +RIRLGNLL +TSQGTAF+ AGQ Sbjct: 1008 GDMVQYIEAHDNLPLYDIIAQSIKKDPSIAANDLEIHKRIRLGNLLTLTSQGTAFLHAGQ 1067 Query: 659 EYGRTKQWKAAGVPEQKYTEMKDANGQSFGYFIHDSYDSSDAVNMFDWAKATDEVNYPVQ 718 EYGRTKQW GVPEQKY E++D G FGYFIHDSYDSSDA+NMFDW KATDE YP Sbjct: 1068 EYGRTKQWLGPGVPEQKYHELEDEAGNRFGYFIHDSYDSSDAINMFDWEKATDEAKYPEN 1127 Query: 719 NTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAPEIKATDLVIGYKNKATDGTGLY 778 N TR YT+GLI+LRKS+DAFRLGD+S+V++NV L+ PE+KA DLVI YKNKATDGTG Y Sbjct: 1128 NKTREYTAGLIELRKSSDAFRLGDQSMVNSNVTLLQIPEVKAQDLVIAYKNKATDGTGHY 1187 Query: 779 YVFMNGDDAARTLTLSEDLTGGQVLVDNDQAGVTAIPAESQSGFTLTSTSITLDPLTAVV 838 YVF+N D+ +RTLTLSEDL+ G V+VDND+AG T + A +SGFTLTS SITL+PLTAVV Sbjct: 1188 YVFVNADNQSRTLTLSEDLSSGTVVVDNDEAGTTGVKA--KSGFTLTSGSITLEPLTAVV 1245 Query: 839 IRKDAAAAVLASLGTDSTGYALQAGSTHQTAVTAKYDDGSARTVTNAAAYISSNPAVAAV 898 I+ DAAA+VL ++ D Y+LQ G THQTAV A+Y+DGS R VT A Y SSNP VA+V Sbjct: 1246 IKTDAAASVLRAISVDQESYSLQTGKTHQTAVYAEYEDGSKRAVTKQATYASSNPQVASV 1305 Query: 899 TSKGLVKGVKPGTATITITYGGLSAKVNVEVTKEPVDTKRYVQITYVRDDKDYADWNLWV 958 T+ G+VKGV GTA IT++Y G ++ V VT + VD KRYVQ+ Y+R D+DYADWNLWV Sbjct: 1306 TAAGVVKGVAEGTAVITVSYRGFQKEITVTVTADEVDNKRYVQLNYIRPDQDYADWNLWV 1365 Query: 959 WNTGVKNDQIDFTTFKDGKASVLIEVAPNATSVGFVLRKGTDWNTGKQDYPDDRVIPLTA 1018 WNTGVKNDQIDF ++G A+V IEVAP T VGFVLRKGT+W+T KQD PDDR+IPL Sbjct: 1366 WNTGVKNDQIDFEKIENGVATVKIEVAPETTGVGFVLRKGTNWDTAKQDIPDDRIIPLQP 1425 Query: 1019 GGGVXXXXVNVTSMVKELDIKPVINGPVMKDGTITFRYRDDALFRTDQQETISGVKVKVN 1078 G VNVTSMV EL+I P I GP ++ G ITF YRDD LFR + ++IS VKVK+N Sbjct: 1426 GEAFTK--VNVTSMVHELEILPSIAGPTLEGGNITFHYRDDQLFRDGRMDSISDVKVKIN 1483 Query: 1079 GKEYPMAYEAAEEWFSYKLQDVQEGTYKYSFLVTKDGVTEELTDPKNTVNGESVVVYHIP 1138 GK M Y +EWFSY L+ V GTY+Y+F+V G T+E+TDPKNTVNG+S VVY P Sbjct: 1484 GKLETMVYVPDKEWFSYTLKGVAPGTYEYTFIVNHKGTTKEVTDPKNTVNGKSQVVYRNP 1543 Query: 1139 KVAITAEVQPEAINSNENAVVTVKAASEEQVSYVDGYMDLSALGGPEAVKLDTELLQQTV 1198 V I A+V P +I+ +ENAV+ + S E V+Y + ++DL+ LGGP K+DT LL+ ++ Sbjct: 1544 TVTIGAKVTPGSISYDENAVLKLNVTSSEPVAYKEAFVDLTELGGPARAKVDTGLLEHSI 1603 Query: 1199 AVKNSVPAGLKSIPITLLDQYGNTHKGTAQIEVKARTYTGGQLDFDWDEARIYFALTDRF 1258 AVK++V AG K I ITL+DQYGN H G A + VK R GG+LDFDWDEARIYF LTDRF Sbjct: 1604 AVKDTVTAGSKKIGITLVDQYGNRHTGEAAVTVKPRI-AGGKLDFDWDEARIYFVLTDRF 1662 Query: 1259 KDGDATNNADVDTTHPEAYHGGDFRGMIDSLDYLQKLGINTLWITPVVDNIDFNKGADFG 1318 DGD +NNA+VD HPEAYHGGDFRG+ID LDY++ LG+NTLWITP+VDNIDFN+GA FG Sbjct: 1663 MDGDVSNNANVDKDHPEAYHGGDFRGLIDKLDYIEDLGVNTLWITPIVDNIDFNQGAGFG 1722 Query: 1319 GTQYGYHGYWAKDFTQLDEHLGDMATFKELIEKAHDKGIKIMVDVVLNHAGYGLKAEDNY 1378 G QY YHGYWAKDFT++DEHLGD+ TFKELI+KAHD+GIKIMVDVV+NH GYGLK D+ Sbjct: 1723 GKQYAYHGYWAKDFTKIDEHLGDLDTFKELIDKAHDRGIKIMVDVVVNHTGYGLKPGDSA 1782 Query: 1379 PGVTAEDKARFDGMLRKDGVSADTDPIKGELAGLPDFKTEDPAVRQTLIGWQTGWLDNAR 1438 PG+T EDK RF MLR DG+SADTDPIKGELAGLPDF TE+PAVRQ +I WQ WL+ A+ Sbjct: 1783 PGITQEDKDRFKDMLRTDGLSADTDPIKGELAGLPDFITENPAVRQQIIDWQASWLERAK 1842 Query: 1439 TERGDTIDYFRVDTVKHVDSTTWKAFKNALTAIDPAFKLTGEYFGGTLDNDGGNLHSGQM 1498 T+RGDTIDYFRVDTVKHVD TTWKAFKNALT +DP+FK+ GEYFG T+D+DGG L SGQM Sbjct: 1843 TDRGDTIDYFRVDTVKHVDETTWKAFKNALTHLDPSFKMIGEYFGATIDSDGGTLQSGQM 1902 Query: 1499 DGLLDFGFKNAAKDFTDGKISAVDTYLQHREAQMDNTGMMAQFLSSHDENGFLSNYVDGD 1558 D LLDFGFK AK+F +G + +V+ YL RE+++DNT MM QFLSSHDE+GFLS+YVDG+ Sbjct: 1903 DSLLDFGFKGMAKEFVNGSVESVEAYLADRESKLDNTRMMGQFLSSHDEDGFLSHYVDGN 1962 Query: 1559 KSKLKIAAALQITAKGQPVIYYGEELGRSGANSGDLSKGEFSENRGDMPWDQLSAEQGLH 1618 K+KL AAALQIT+KGQPVIYYGEELGRSG N+GD+ KGEFSENRGDMPWDQL AE+ L Sbjct: 1963 KAKLMAAAALQITSKGQPVIYYGEELGRSGKNAGDMGKGEFSENRGDMPWDQLEAEKKLL 2022 Query: 1619 DHYQKLLNIRAKYSQIYSKGIRTKLAGSDELGYLAFDKQYDGANIVTAINTKAEAVSVSL 1678 HYQKLLNIRA S+++SKG RT +A S+ GYLAFDKQY+G +IVTAI+ K E V L Sbjct: 2023 THYQKLLNIRAANSKVFSKGTRTSIAASNADGYLAFDKQYNGEHIVTAIHVKNEPKEVVL 2082 Query: 1679 PVPFAAQAAVVDEYSGKTYTVSGDQKVRVELPGRGDGGTVILAVVPKVXXXXXXXXXXXX 1738 VPF A + D Y G Y V+ DQKV + LP DGGTVIL VP Sbjct: 2083 SVPFKAGSKAKDLYGGSVYNVTADQKVTLTLPAMSDGGTVILTAVP---AEGGGNGSNPG 2139 Query: 1739 XXXXXXXXXXXXXXXXXXEPGNGSGWNXXXXXXXXXXXXXQLINGDSLKNGKDGKVTVEL 1798 +P G +++N +SL+ G++ KVT++L Sbjct: 2140 SGNGGGGAGGNPSTDPGTKPNQGQ----------------RIVNEESLRGGQE-KVTIQL 2182 Query: 1799 ASGKXXXXXXXXXXXXXGTNDLVLTMSGLSITLPVQVLRTVQGTLTGADAEGAQIXXXXX 1858 + G L L G+ + LP +L+ ++ + A+ A I Sbjct: 2183 GAEDIEVLLPLTAGDILGAKPLELKQDGVMVQLPSALLKALRQLVPAEQAKDAVISFKLK 2242 Query: 1859 XX-XXXXXXXXXXXXXKDNMSVTAASELYEFRLEVIRKDGKRVPVTSFAEPVKLAFQLKG 1917 + S++YE L V KDGK + F PV +AF + Sbjct: 2243 EVGSQTAASLVAKAAAAGKARLKQGSKMYELDLNVRTKDGKAYKLEHFIAPVTIAFPVDN 2302 Query: 1918 NPDKALLGVYYLGDNSSLQYAGGSQQGDIITAGVTHFSTYAALVINKAYSDVPAAYWAET 1977 L G+Y + + +L+YAGG +TA V+ S YA L +K ++D+ A +WAE Sbjct: 2303 AIHGELAGIYGISELGTLKYAGGKPVNGQLTAEVSQLSYYAVLEYDKTFADL-AGHWAEQ 2361 Query: 1978 AIKSLSAKQVITGVTASEFKPGNPVTRAEFTALLVRALSLQTEGQAASQDVRAGEQSGFT 2037 A+K L+AK ++ G ++F P P+TRA+F A+LVRAL L++ +QD Sbjct: 2362 AVKELAAKHIVNGTGDTKFSPHQPLTRAQFAAMLVRALGLES-----TQD---------- 2406 Query: 2038 DVKAGEQNGFTDVKEDAWYASYVSAAVRQGVVTGRSNGLFAPDAVISREEMAVMIIRALE 2097 FTD+ DAWYAS V+AA + G+V GR FAP+A I+REE+AVM+ RA + Sbjct: 2407 ------DTTFTDMDGDAWYASAVAAASQHGLVNGRGKDTFAPNAAITREELAVMLYRAYQ 2460 Query: 2098 LK-QGKKLEPANGGLRFADASSISSWAAEYVSAAAEQGLLQGRAANKFAPEAWMTRAEAA 2156 K LE G D ISSWA + V+ A GL++G A F P+A TRAE+A Sbjct: 2461 TKGDASSLE---GAPNLKDKQRISSWALQEVNEALALGLMKGHANGMFTPQAKTTRAESA 2517 Query: 2157 QVIYRLLGK 2165 Q + LL + Sbjct: 2518 QAMLNLLDR 2526 Score = 58.9 bits (141), Expect = 7e-06, Method: Compositional matrix adjust. Identities = 28/51 (54%), Positives = 37/51 (72%), Gaps = 1/51 (1%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLD-RYNQLWIKQGDDHVY 50 VD+PLK AQK+G +VV+R SG+KDGGDK L N++W+K+G D VY Sbjct: 304 VDIPLKDGAQKVGIVVVDRISGNKDGGDKKLTLASPDMNEVWLKEGSDTVY 354 >Q2BFA5.1 Q2BFA5_9BACI Alkaline amylopullulanase Length = 2249 Score = 1129 bits (2921), Expect = 0.0, Method: Compositional matrix adjust. Identities = 553/843 (65%), Positives = 648/843 (76%), Gaps = 11/843 (1%) Query: 2 DVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGEQPVSL 61 D+ L D +++GFL+VN S+G+KD GDKTF LLD+Y+QLWIKQGDD VY+SP+G+ + Sbjct: 992 DIELNEDGKELGFLIVNESTGEKDAGDKTFNLLDKYSQLWIKQGDDVVYISPYGDVASGM 1051 Query: 62 VSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTSVEVDTAAF 121 SAE++S ILL FT+T+GL A GLK+ +++TDK+G + + +A I + EV A F Sbjct: 1052 TSAEVISEETILLNFTMTEGLTADGLKAGLSITDKDGKEISITSAKITGDKTAEV-KAVF 1110 Query: 122 ALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPMASSVV 181 LEK PLSV YSG+ VSAS+GWRM+D++Y+Y GDDLGATY + A LKLWAP AS V Sbjct: 1111 DLEKLPLSVTYSGRTVSASSGWRMLDKLYSYDGDDLGATYK--NGRAVLKLWAPTASKVT 1168 Query: 182 ANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNGVTK 241 AN Y K DAA+ +G V L LGDKGVWS E++PS+L+ +D++GYYYQYEVTNNGVTK Sbjct: 1169 ANFYDKKDAAKLMGSVDLELGDKGVWSKEIQPSELN----VDDLKGYYYQYEVTNNGVTK 1224 Query: 242 PVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKREDAVI 301 VLDPYAKSMA FTV++ G AGPDGD VGKAAI+DL GT+P + I GYEKREDA+I Sbjct: 1225 KVLDPYAKSMAAFTVNTKGEAGPDGDDVGKAAIIDLKGTDPNGFDHASIKGYEKREDAII 1284 Query: 302 YEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNGDETRM 361 YEVHVRDFTSD SIE++L RWGSY AF KLDYIKS G+TH+QL+PVMAWY GDET+M Sbjct: 1285 YEVHVRDFTSDPSIEADLNS-RWGSYKAFIDKLDYIKSTGVTHVQLLPVMAWYFGDETQM 1343 Query: 362 THRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMGVIL 421 RE YS NEYNWGYDPHSYFSPDGAYS+ P D E R+KELK LIDA+H+AGMGV+L Sbjct: 1344 GEREMEYSAGGNEYNWGYDPHSYFSPDGAYSENPEDAELRVKELKELIDAIHDAGMGVVL 1403 Query: 422 DVVYTHMAKKDFLDDIVPNYYAFQDAXXXXXXXXXXXLATSHKMAEKLMVDSVKYWFSEY 481 DVVYTHMAK L+DIVPNYYAFQDA LATSH MAEKLMVDSVKYWF EY Sbjct: 1404 DVVYTHMAKASTLNDIVPNYYAFQDAKGNFLGGFGNNLATSHTMAEKLMVDSVKYWFDEY 1463 Query: 482 KIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGADQAW 541 KIDGMR+DMMG INP ALFIGEGW+TFAG +DPAL G GADQ W Sbjct: 1464 KIDGMRFDMMGDATYPSIQNAYDAAAAINPDALFIGEGWRTFAGHLADPALEGMGADQDW 1523 Query: 542 MDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPADDPGDV 601 MDKTD VGVFSDE RNELKSG+GSEGEPRFITGGAR + IFNNI +PSN DDPGD+ Sbjct: 1524 MDKTDDVGVFSDEIRNELKSGFGSEGEPRFITGGARDLGVIFNNIKGQPSNTADDDPGDM 1583 Query: 602 VPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQAGQEYG 661 V YIEAHDNL L+DVIAQ+IKKDP+IP N LEI +RIRLGN L +TSQGTAF+ AGQEYG Sbjct: 1584 VQYIEAHDNLPLYDVIAQSIKKDPAIPENNLEIHKRIRLGNSLILTSQGTAFLHAGQEYG 1643 Query: 662 RTKQWKAAGVPEQKYTEMKDANGQSFGYFIHDSYDSSDAVNMFDWAKATDEVNYPVQNTT 721 RTKQW A GVPEQKY E+ D NG FGYFIHDSYDSSDA+N FDW KAT+ Y V T Sbjct: 1644 RTKQWLAEGVPEQKYHELTDENGVPFGYFIHDSYDSSDAINKFDWQKATNASAYKVNTMT 1703 Query: 722 RAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAPEIKATDLVIGYKNKATDGTGLYYVF 781 R YT GLI+LR+ST+AFRLGDK LVD NV+L++ PE+K DL+I YKN +TD TG YYVF Sbjct: 1704 REYTEGLIELRRSTNAFRLGDKELVDKNVSLLSFPEMKDQDLLIAYKNVSTDKTGSYYVF 1763 Query: 782 MNGDDAARTLTLSE-DLTGGQVLVDNDQAGVTAIPAESQSGFTLTSTSITLDPLTAVVIR 840 MN D R+LTL E DL+ G VLVDND+AG+ A+ + +SGFTL +TLDPLT VVI+ Sbjct: 1764 MNADSKVRSLTLGELDLSKGTVLVDNDEAGIKAV--KDKSGFTLADGKLTLDPLTVVVIK 1821 Query: 841 KDA 843 + A Sbjct: 1822 EGA 1824 Score = 699 bits (1804), Expect = 0.0, Method: Compositional matrix adjust. Identities = 367/800 (45%), Positives = 524/800 (65%), Gaps = 28/800 (3%) Query: 936 TKRYVQITYVRDDKDYADWNLWVWNTGVKNDQIDFTTFKDGKASVLIEVAPNATSVGFVL 995 ++R V+ TY+RDDK Y WN+W WNTGVK+DQI+F ++++GKA+V I VAP GFVL Sbjct: 22 SERQVRFTYIRDDKAYEGWNIWAWNTGVKDDQINFQSYENGKATVNIAVAPETKQFGFVL 81 Query: 996 RKGTDWNTGKQDYPDDRVIPLTAGGGVXXXXVNVTSMVKELDIKPVINGPVMKDGTITFR 1055 R +WNT ++++ DR I + + + TS V+++ I P + PV+ G TF Sbjct: 82 RSTENWNTAEKEF-GDRFITVNKNDSLTKAYI--TSGVEKIRIVPDGSAPVINKGNATFY 138 Query: 1056 YRDDALFRTDQQETISGVKVKVNGKEYPMAYEAAEEWFSYKLQDVQEGTYKYSFLVTKDG 1115 YRD LF D +I V++K NG+ M YE E F +++ G ++YS+LVTKDG Sbjct: 139 YRDKELFANDSMNSIEKVELKFNGETQEMVYEPENERFMLTYENIPNGKHEYSYLVTKDG 198 Query: 1116 VTEELTDPKNTVNGESVVVYHIPKVAITAEVQPEAINSNENAVVTVKAASE-EQVSYVDG 1174 T E+TDP NT +G S + + + ++A +P AI+ ++NAV++V A + ++V Sbjct: 199 KTIEVTDPYNTKDGISAIDFQQADLTVSASTRPAAIDYSQNAVLSVDIAGQTDEVKITKI 258 Query: 1175 YMDLSALGGPEAVKLDTELLQQTVAVKNSVPAGLKSIPITLLDQYGNTHKGTAQIEVKAR 1234 D S+LGGP+ +++D L + T+A +S AG+K IP+T +D YGN + G A+IE+K R Sbjct: 259 LADTSSLGGPDQLEIDPSLGKITIAADDSTTAGVKKIPVTAVDSYGNEYSGNAEIEIKTR 318 Query: 1235 TYTGGQLDFDWDEARIYFALTDRFKDGDATNNADVDTTHPE---AYHGGDFRGMIDSLDY 1291 + G+ DFDWDE+ IYF LTDRF DG+++NN + + AY GGDF+G+ + LDY Sbjct: 319 Q-SAGEGDFDWDESVIYFMLTDRFFDGNSSNNDPYGLNYDDSRGAYQGGDFKGVTEKLDY 377 Query: 1292 LQKLGINTLWITPVVDNIDFN---KGADFGGTQYGYHGYWAKDFTQLDEHLGDMATFKEL 1348 L +LG+NT+WI+PVV+NI ++ AD G Y YHGYWA++F++L+ H G M F L Sbjct: 378 LDELGVNTIWISPVVENIQYDVRDSEAD-GEPYYAYHGYWAENFSKLNPHFGTMEDFHTL 436 Query: 1349 IEKAHDKGIKIMVDVVLNHAGYGLK-----AEDNYPGVTAE-DKARFDGMLRKDGVSADT 1402 I++AH++GIKIMVDVVLNH GYGLK E+ PG + D++RF ++R+ V + Sbjct: 437 IDEAHERGIKIMVDVVLNHTGYGLKELDAGKENPPPGYPDDGDRSRFKDIVRQGNVG--S 494 Query: 1403 DPIKGELAGLPDFKTEDPAVRQTLIGWQTGWLDNARTERGDTIDYFRVDTVKHVDSTTWK 1462 D + GEL+ LPD KTEDPAVR+ +I WQT W++ A T G+TIDYFRVDTVKHV+ TW Sbjct: 495 DEVVGELSSLPDIKTEDPAVRKQIIDWQTDWIEKATTADGNTIDYFRVDTVKHVEDATWM 554 Query: 1463 AFKNALTAIDPAFKLTGEYFGGTLDNDGGNLHSGQMDGLLDFGFKNAAKDFTDGKISAVD 1522 +FKNALT PAFK+ GE +G + DN+ G L SG MD LLDFGFK A++F +GK+ A + Sbjct: 555 SFKNALTEKMPAFKMIGEAWGASADNNLGYLDSGMMDSLLDFGFKETARNFVNGKLKAAN 614 Query: 1523 TYLQHREAQMDNTGMMAQFLSSHDENGFLSNYVDGDKSKLKIAAALQITAKGQPVIYYGE 1582 L R A++DNT + QFLSSHDE+GFL + + GDK K+K+AA+LQ TAKGQPVIYYGE Sbjct: 615 DALAARNAKVDNTATLGQFLSSHDEDGFLHS-LGGDKGKMKVAASLQATAKGQPVIYYGE 673 Query: 1583 ELGRSGANSGDLSKGEFSENRGDMPWDQLSAEQGLHDHYQKLLNIRAKYSQIYSKGIRTK 1642 ELG++GAN+ +NR D WD++ + + +HY+K+LN R +S++++KG RT Sbjct: 674 ELGQTGANNYPQ-----YDNRYDFAWDRVE-DNDMLEHYKKILNFRKDHSKVFAKGDRTV 727 Query: 1643 LAGSDELGYLAFDKQYDGANIVTAINTKAEAVSVSLPVPFAAQAAVVDEYSGKTYTVSGD 1702 GSD+ +L F + Y G + +NT +A ++ L V A A V D YS + Y + Sbjct: 728 TGGSDKEQFLLFARSYGGQTVYAGLNTADKAKTIKLAVDSKA-ATVTDHYSDQNYQTTEA 786 Query: 1703 QKVRVELPGRGDGGTVILAV 1722 +V + +P DGGTV+L V Sbjct: 787 GEVILTIPANSDGGTVLLTV 806 Score = 115 bits (288), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 70/217 (32%), Positives = 111/217 (51%), Gaps = 27/217 (12%) Query: 1955 STYAALVINKAYSDVPAAYWAETAIKSLSAKQVITGVTASEFKPGNPVTRAEFTALLVRA 2014 S Y + +K + DV + WAE I++L++K +I G T ++ PG +TRAEFT LLVRA Sbjct: 2049 SVYGIVENDKTFQDVNRSSWAEEYIETLASKYIIKGKTDEKYAPGEEITRAEFTVLLVRA 2108 Query: 2015 LSLQTEGQAASQDVRAGEQSGFTDVKAGEQNGFTDVKEDAWYAS--YVSAAVRQGVVTGR 2072 L L A + D + F+DVK + W+ + ++AAV G+++G+ Sbjct: 2109 LGL----PAKAYDKK-----------------FSDVKGNEWFNANGELTAAVHFGIISGK 2147 Query: 2073 SNGLFAPDAVISREEMAVMIIRALELK----QGKKLEPANGGLRFADASSISSWAAEYVS 2128 +G FAP+ ++R + AVMI RA++L +L F DAS +WA E Sbjct: 2148 PDGTFAPNEKLTRAQAAVMIARAMDLGFLEFDKSQLNSNKKLTDFKDASQFGAWAKEGAE 2207 Query: 2129 AAAEQGLLQGRAANKFAPEAWMTRAEAAQVIYRLLGK 2165 A + G++ G F P + R + A+++ L K Sbjct: 2208 AVYQSGIMSGMTKTTFNPNGYTKRDQMAKILAEFLRK 2244 >P70983.1 P70983_BACSP Alkaline amylopullulanase Length = 1938 Score = 850 bits (2195), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/843 (51%), Positives = 570/843 (67%), Gaps = 27/843 (3%) Query: 2 DVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGEQPVSL 61 D+ LK DA KIGFL VN+ SG + G D TF +L +YNQL++K+G+D VY +P+G P++L Sbjct: 993 DIKLKEDANKIGFLFVNKQSGGQTG-DMTFDMLKQYNQLFVKEGEDKVYTNPYGTVPLAL 1051 Query: 62 VSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTSVEVDTAAF 121 VS E+LS + I LTFT T+GLD LK + + D +G V I+ +V V F Sbjct: 1052 VSGEVLSDKLISLTFTRTEGLDLEELKEQLEIKDVDGNDVSFTDVTIEGEKTVHVH-GEF 1110 Query: 122 ALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPMASSVV 181 LEK P SV Y + +S +GW++IDEMY Y G LGA H D +ATLK+W+P A +V Sbjct: 1111 DLEKIPFSVTYLDRTISVKSGWKLIDEMYAYDGK-LGAELH-EDGTATLKVWSPKADNVS 1168 Query: 182 ANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNGVTK 241 +Y K D + V + + GD+GVWS +L + + ++GYYY YE+T+ VT Sbjct: 1169 VVLYDKVDQNEVVDTIEMVKGDRGVWSVKLTKDN----TGLDSLKGYYYHYEITHGDVTN 1224 Query: 242 PVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKREDAVI 301 LDPYAKSMA + ++ GD VGKAAIVD+ G+ P+ DIPG+EKRED +I Sbjct: 1225 LALDPYAKSMAAWNNEA-------GDKVGKAAIVDI-GSIGPELDYADIPGFEKREDTII 1276 Query: 302 YEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNGDETRM 361 YEVHVRDFTSD +I +L + +G++A+F +KLDYI+ LG+THIQL+PVM++Y +E Sbjct: 1277 YEVHVRDFTSDPNIGEDLKAQ-FGTFASFVEKLDYIQELGVTHIQLLPVMSYYFSNEFES 1335 Query: 362 THRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMGVIL 421 R Y++ YNWGYDPH+YFS G YS+ P DPE RIKE K LI+ +H+ MGV+L Sbjct: 1336 GERMLEYASTGTNYNWGYDPHNYFSLSGMYSENPEDPELRIKEFKNLINEIHKRDMGVVL 1395 Query: 422 DVVYTHMAKKDFLDDIVPNYYAFQDAXXXXXXXXXX-XLATSHKMAEKLMVDSVKYWFSE 480 DVV+ H A+ +D+VPNYY F DA L T+H+M+ +++VDS+K+W E Sbjct: 1396 DVVFNHTAQVHIFEDLVPNYYHFMDADGTPRTSFGGGRLGTTHEMSRRVLVDSIKHWVDE 1455 Query: 481 YKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGADQA 540 YK+DG R+DMMG +NP + IGEGW TFAGD +P A ADQ Sbjct: 1456 YKVDGFRFDMMGDHDAESIQIAFDEAKKLNPNIVMIGEGWVTFAGDEGEPVQA---ADQQ 1512 Query: 541 WMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPADDPGD 600 WM T++VG FSDEFRNELKSG+GSEG+PRFITGGA ++ IF+NI A+P N AD PGD Sbjct: 1513 WMQYTEAVGSFSDEFRNELKSGFGSEGQPRFITGGAVNVQQIFDNIKAQPHNFMADQPGD 1572 Query: 601 VVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQAGQEY 660 VV YIEAHDNLTL+DVIAQ+IKKDP I N+LEI +RIR+GN + +TSQGTAF+ AGQE+ Sbjct: 1573 VVQYIEAHDNLTLYDVIAQSIKKDPEIAENDLEIHKRIRVGNAMVLTSQGTAFLHAGQEF 1632 Query: 661 GRTKQWKA-AGVPEQKYTEMKDANGQSF--GYFIHDSYDSSDAVNMFDWAKATDEVNYPV 717 GRTKQW+A A K T M DA+G F YFIHDSYDSSD +N FDW KATD YPV Sbjct: 1633 GRTKQWRAPATEAPYKSTYMTDADGNPFVYPYFIHDSYDSSDIINRFDWEKATDAEKYPV 1692 Query: 718 QNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAPEIKATDLVIGYKNKATDGTGL 777 N TR YT+GLI+LR+S+DAFRLG + LVD+NV ++ APEIK DLV+ Y++ +T G Sbjct: 1693 NNVTRDYTAGLIELRRSSDAFRLGSRELVDSNVTMVDAPEIKEQDLVVAYRSVSTAGVE- 1751 Query: 778 YYVFMNGDDAARTLTLSEDLTGGQVLVDNDQAGVTAIPAESQSGFTLTSTSITLDPLTAV 837 YY F+N D ++RTLTL +DLT G V+VD ++A V + +GF LT+ ITL+PLT V Sbjct: 1752 YYTFVNADTSSRTLTLGQDLTEGVVVVDAEEANVAGVA--EPAGFELTAEGITLEPLTTV 1809 Query: 838 VIR 840 V+R Sbjct: 1810 VVR 1812 Score = 710 bits (1833), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/798 (47%), Positives = 522/798 (65%), Gaps = 34/798 (4%) Query: 938 RYVQITYVRDDKDYADWNLWVWNTGVKNDQIDFTTFKDGKASVLIEVAPNATSVGFVLRK 997 + ++ +Y R D +Y WNLWVW TGVK+DQIDFT FK+GKA I V+ NA VGF++RK Sbjct: 37 KRIEFSYERPDGNYEGWNLWVWGTGVKDDQIDFTEFKEGKAYADIAVSDNADKVGFIIRK 96 Query: 998 GTDWNTGKQDYPDDRVIPLTAGGGVXXXXVNVTSMVKELDIKPVINGPVMKDGTITFRYR 1057 G DW ++D+ DR I + + V+VTS ++ P + PV+ DG F +R Sbjct: 97 G-DWE--EKDFDGDRSITINKIDNITK--VHVTSQQEKFGQIPDGSPPVVADGNADFFFR 151 Query: 1058 DDALFRTDQQETISGVKVKVNGKEYPMAYEAAEEWFSYKLQDVQEGTYKYSFLVTKDGVT 1117 D L+ + + + V++ + G++Y M E +E F+Y L D+ G ++Y++LVT DG T Sbjct: 152 DKELYAAGEMDKVEKVELSILGEKYEMNGEPEKERFTYTLSDLPTGEHEYTYLVTVDGQT 211 Query: 1118 EELTDPKNTVNGESVVVYHIPKVAITAEVQPEAINSNENAVVTVKAASEEQVSYVDGYMD 1177 EE+TDP NTV+G SVV Y V ++A P ++ N+NAVV V SE + + ++ Sbjct: 212 EEVTDPYNTVDGRSVVEYVTSDVQVSASFIPAKVDYNQNAVVKVDIESETETKIREMSIN 271 Query: 1178 LSALGGPEAVKLDTELLQQTVAVKNSVPAGLKSIPITLLDQYGNTHKGTAQIEVKARTYT 1237 LS +GG E +D L + TVAVK V AG+K++PIT +D++GN H+G+A +EV+ART T Sbjct: 272 LSEIGGKEKATIDPALNELTVAVKQGVTAGVKNLPITAIDEFGNRHEGSATLEVQARTIT 331 Query: 1238 GGQLDFDWDEARIYFALTDRFKDGDATNN----ADVDTTHPEAYHGGDFRGMIDSLDYLQ 1293 G + DFDWD++ +YF LTDRF DGD++NN DT+ Y GGDF+G+ LDYL Sbjct: 332 GEKADFDWDQSVVYFMLTDRFFDGDSSNNDPHGIGYDTSKSGTYQGGDFKGITQRLDYLD 391 Query: 1294 KLGINTLWITPVVDNIDFN----KGADFGGTQYGYHGYWAKDFTQLDEHLGDMATFKELI 1349 +LGINT+WI+PVVDNI F+ +G D Y YHGYWA +F +L+ H G MA F E+I Sbjct: 392 ELGINTIWISPVVDNIKFDVRHSEGPD--TPYYAYHGYWADNFGELNPHFGSMADFHEMI 449 Query: 1350 EKAHDKGIKIMVDVVLNHAGYGLKAED-----NYPGVTAEDKARFDGMLRKDGVSADTDP 1404 + AH++GIKIMVDVVLNH GYGLK D N+P T ED+ARFDGMLR DG S + Sbjct: 450 DAAHERGIKIMVDVVLNHTGYGLKPGDSSSVANFP--TDEDRARFDGMLR-DGGSGE--- 503 Query: 1405 IKGELAGLPDFKTEDPAVRQTLIGWQTGWLDNARTERGDTIDYFRVDTVKHVDSTTWKAF 1464 ++GELAGLPDF TE+P VR+ ++ WQT W++ +RT +G+TIDYFRVDTVKHV+ TTW AF Sbjct: 504 VRGELAGLPDFLTENPDVREQVVQWQTDWIEKSRTAKGNTIDYFRVDTVKHVEDTTWMAF 563 Query: 1465 KNALTAIDPAFKLTGEYFGGTLDNDGGNLHSGQMDGLLDFGFKNAAKDFTDGKISAVDTY 1524 KNALT P KL GE +G +++D G L+SG MD LLDF FKN A+DF +G++ AV Sbjct: 564 KNALTKAMPEHKLIGEAWGANVNDDLGYLNSGMMDSLLDFDFKNYARDFANGQLDAVQQK 623 Query: 1525 LQHREAQMDNTGMMAQFLSSHDENGFLSNYVDGDKSKLKIAAALQITAKGQPVIYYGEEL 1584 L+ R ++++NT + QFL SHDE+ F V+GD K ++AA+LQ+TAKGQPVIYYGEEL Sbjct: 624 LEARNSKLNNTATLGQFLGSHDEDRFYE-VVEGDLGKYQVAASLQLTAKGQPVIYYGEEL 682 Query: 1585 GRSGANSGDLSKGEFSENRGDMPWDQLSAEQGLHDHYQKLLNIRAKYSQIYSKGIRTKLA 1644 G G N + NR +MPWD + + L +HYQKLL R ++KG R K+A Sbjct: 683 GLPGKND-----YPYYTNRQNMPWDDVDGNEIL-EHYQKLLAFRNDNPNTFAKGDRKKVA 736 Query: 1645 GSDELGYLAFDKQYDGANIVTAINTKAEAVSVSLPVPFAAQAAVVDEYSGKTYTVSGDQK 1704 GSD GYL F + Y ++ +NT+A A V+L +++A V D YSG+ Y + + + Sbjct: 737 GSDSEGYLLFSRTYGENSVYVGLNTEAAAKDVTLNFG-SSEAVVTDRYSGQEYQANEEGQ 795 Query: 1705 VRVELPGRGDGGTVILAV 1722 V +P DGGTV+L V Sbjct: 796 VTFSIPAMEDGGTVLLEV 813 Score = 62.8 bits (151), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 33/62 (53%), Positives = 41/62 (66%), Gaps = 5/62 (8%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLD-RYNQLWIKQGDDHVYVSPFGEQPV 59 VDV L DAQ IGFLV+N ++GDKDGGDK L N++WIKQG D V++ +PV Sbjct: 884 VDVELAEDAQNIGFLVLNTTNGDKDGGDKAVELFSPDLNEIWIKQGSDEVFLY----EPV 939 Query: 60 SL 61 L Sbjct: 940 DL 941 >Q9K6N1.1 Q9K6N1_BACHD Alkaline amylopullulanase Length = 1072 Score = 827 bits (2135), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/846 (51%), Positives = 559/846 (66%), Gaps = 28/846 (3%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGEQPVS 60 +DV L DA +IGFLVVNR +GDKDG DKTF L + QL+I+QGDD VY +P+ + Sbjct: 199 LDVELADDANEIGFLVVNRENGDKDGDDKTFADLANHPQLFIRQGDDTVYTNPYYQTIEG 258 Query: 61 LVSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTSVEVDTAA 120 LVS E++S ++ L FT T L L I +TD G V + I +V + + Sbjct: 259 LVSGELISLTEMELRFTSTIWLSEDDLAEQIHLTDTNGASVAFDHVTIHDEKTV-ILHGS 317 Query: 121 FALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPMASSV 180 F E AP V ++ K V A AGWR+ID +Y Y G+ LGA H D S+TL W+P+A V Sbjct: 318 FDAELAPYHVTFADKTVVARAGWRLIDALYGYDGE-LGAVLH-EDGSSTLTFWSPLADHV 375 Query: 181 VANVYSKTDAAQQV-GQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNGV 239 +Y K D + + + +T GD+GVW L S+ +++ GYYY Y++ +G Sbjct: 376 SVVLYDKDDQYRIIKDDIDMTKGDQGVWQVTLH----SENTGLDNLAGYYYHYKIERDGD 431 Query: 240 TKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKREDA 299 TK LDPYAKSMA + + + + +GKAAIVD S P + +I G+EKREDA Sbjct: 432 TKLGLDPYAKSMAAWNNEGSYS-------IGKAAIVDPSSIGP-ELDFAEIEGFEKREDA 483 Query: 300 VIYEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNGDET 359 +IYEVHVRDFTSD IE EL + +G++A+F KLDYI+ LG+THIQL+PVM++Y GDE Sbjct: 484 IIYEVHVRDFTSDPGIEEELTAQ-FGTFASFVDKLDYIEDLGVTHIQLLPVMSYYWGDEL 542 Query: 360 RMTHRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMGV 419 RE YS+ N YNWGYDPHSYFS G YS+ P DPE RIKE K LID +H GMGV Sbjct: 543 ANHERELAYSSSGNNYNWGYDPHSYFSLSGMYSENPEDPELRIKEFKHLIDEIHRRGMGV 602 Query: 420 ILDVVYTHMAKKDFLDDIVPNYYAFQDAX-XXXXXXXXXXLATSHKMAEKLMVDSVKYWF 478 ILDVVY H A + +D++PNYY F DA L T+HKM+ +++VDS+ YW Sbjct: 603 ILDVVYNHTAAVEIFEDLMPNYYHFMDADGTPRTSFGGGRLGTTHKMSRRILVDSITYWV 662 Query: 479 SEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGAD 538 EYK+DG R+DMMG +NP + IGEGW+T+ GD + AD Sbjct: 663 EEYKVDGFRFDMMGDHDAETIQIAYDRAKELNPNIVMIGEGWRTYVGDEHGGDIM--PAD 720 Query: 539 QAWMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPADDP 598 Q WM T+SVGVFSDEFRNELKSG+GSEG+PRFITGGAR+I IF+N+TA+P N A +P Sbjct: 721 QDWMQHTESVGVFSDEFRNELKSGFGSEGQPRFITGGARNIEQIFDNLTAQPHNFTATNP 780 Query: 599 GDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQAGQ 658 GDVVPYIEAHDNLTLHDVIAQ+I+KDP ++ EI +RIRLGN + +T+QGTAF+ AGQ Sbjct: 781 GDVVPYIEAHDNLTLHDVIAQSIRKDPEY--HQEEIHKRIRLGNTMVLTAQGTAFLHAGQ 838 Query: 659 EYGRTKQWKA--AGVPEQKYTEMKDANGQSF--GYFIHDSYDSSDAVNMFDWAKATDEVN 714 E+GRTKQ++A G K T M D NG+ F YFIHDSYDSSDA+N FDW KAT++ Sbjct: 839 EFGRTKQFRADTGGQAPYKSTYMTDENGKPFVYPYFIHDSYDSSDAINKFDWGKATNKEK 898 Query: 715 YPVQNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAPEIKATDLVIGYKNKATDG 774 YP+ + TR YT+GLI+LR+ST+AF G V NV L+ PEI TDLVIGY+ +TD Sbjct: 899 YPIHHLTRTYTAGLIELRRSTNAFTHGTLVDVQANVRLLDIPEISETDLVIGYRATSTDN 958 Query: 775 TGLYYVFMNGDDAARTLTLSEDLTGGQVLVDNDQAGVTAIPAESQSGFTLTSTSITLDPL 834 TG YYVF+N D RTL+L++DLT G V+VD+D+AG+ A+ SGF LT IT+DPL Sbjct: 959 TGTYYVFVNADTKERTLSLTDDLTEGVVIVDSDEAGIVAV--SDPSGFDLTPNEITIDPL 1016 Query: 835 TAVVIR 840 TAV+IR Sbjct: 1017 TAVIIR 1022 >Q93UZ9.1 Q93UZ9_9BACI Alkaline pullulanase Length = 1142 Score = 808 bits (2086), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/845 (50%), Positives = 556/845 (65%), Gaps = 29/845 (3%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGEQPVS 60 +D+PL A++IG +VVN+ + +++ D++F L+++NQ++I +GD VY +P+G Sbjct: 215 IDIPLNEGAEEIGLVVVNKETNEQEA-DRSFTQLNQFNQIFIVEGDGAVYSNPYGAIATE 273 Query: 61 LVSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKS-TTSVEVDTA 119 L+SAE LS KI+L F+ TD L L + + D E V VN IK+ +VEV Sbjct: 274 LLSAEQLSDEKIVLYFSRTDELTEEALLEQVGIRDIEEHTVDVNEVEIKTDNRTVEV-KG 332 Query: 120 AFALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPMASS 179 F L+++P +V+ +V A GWR+IDEMY Y G+ LGA H D +AT+KLW+P A Sbjct: 333 DFNLDQSPYTVSLGEVSVPAKIGWRLIDEMYAYDGE-LGAQLH-QDGTATIKLWSPKAEH 390 Query: 180 VVANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNGV 239 V +Y K + V +V + LGD+GVW +L + + +RGYYY YE+ ++G Sbjct: 391 VQVILYDKDNPDDIVTEVEMKLGDRGVWEVQLTKEN----TGLDSLRGYYYHYEIDHDGD 446 Query: 240 TKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKREDA 299 K LDPYAKS++ + D G KAAIVD S P + I G+EK+ED Sbjct: 447 KKIALDPYAKSLSAWNSDEQGP-------YAKAAIVDPSSIGP-ELEFAHIEGFEKKEDT 498 Query: 300 VIYEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNGDET 359 +IYEVHVRDFTSD I EL + +G++A+F KLDYI+ LG+THIQL+PVM+++ +E Sbjct: 499 IIYEVHVRDFTSDPHIADELTAQ-FGTFASFVDKLDYIEDLGVTHIQLLPVMSYFFANEF 557 Query: 360 RMTHRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMGV 419 + R +S+ N YNWGYDP SYF+ G YS+ P DPE RIKE K LID +H GMGV Sbjct: 558 KNDERMLDFSSTNTNYNWGYDPQSYFALTGMYSEDPTDPELRIKEFKKLIDEIHSRGMGV 617 Query: 420 ILDVVYTHMAKKDFLDDIVPNYYAFQDAX-XXXXXXXXXXLATSHKMAEKLMVDSVKYWF 478 +LDVVY H A+ +D+VPNYY F DA L T+H+M+ +++VDS+ YW Sbjct: 618 VLDVVYNHTARVGIFEDLVPNYYHFMDADGTPRTSFGGGRLGTTHEMSRRILVDSITYWV 677 Query: 479 SEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGAD 538 E+KIDG R+DMMG INP + IGEGW T+AGD DP + + AD Sbjct: 678 EEFKIDGFRFDMMGDHDAESIQIAYDKAKEINPNIVMIGEGWITYAGDEDDPNV--QAAD 735 Query: 539 QAWMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPADDP 598 Q WM T+SVGVFSDEFRNELKSG+G EGEPRF+TGGAR+I IF+NI A+P N ADDP Sbjct: 736 QHWMQYTESVGVFSDEFRNELKSGFGHEGEPRFLTGGARNIDLIFDNIKAQPHNFIADDP 795 Query: 599 GDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQAGQ 658 GDVVPYIEAHDNLTLHDVIA I+KDP ++ EI QRIRLGNL+ +TSQG AF+ AGQ Sbjct: 796 GDVVPYIEAHDNLTLHDVIAMAIQKDPD--DHQEEIHQRIRLGNLMTLTSQGIAFLHAGQ 853 Query: 659 EYGRTKQWKA-AGVPEQKYTEMKDANGQSF--GYFIHDSYDSSDAVNMFDWAKATDEVNY 715 EYGRTKQ++A P K T M D NG+ F YFIHDSYDS+D +N FDW +AT+ Y Sbjct: 854 EYGRTKQFRAETSEPPYKSTYMTDENGEPFRYPYFIHDSYDSTDIINRFDWERATNADAY 913 Query: 716 PVQNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAPEIKATDLVIGYKNKATDGT 775 P+QN TR YT+GLI+LR+S+DAFRLG K LVD V + PEI+ TDLV+ Y+ +AT G Sbjct: 914 PIQNLTREYTTGLIELRRSSDAFRLGTKDLVDEKVTQLNIPEIEETDLVVAYRIEATTGE 973 Query: 776 GLYYVFMNGDDAARTLTLSEDLTGGQVLVDNDQAGVTAIPAESQSGFTLTSTSITLDPLT 835 +YVF+N DD RTLTL EDLT G+ +VD AGVTAI AE Q G L++ I LDPLT Sbjct: 974 A-FYVFVNADDEERTLTLEEDLTVGEFVVDGKTAGVTAI-AEPQ-GVELSAEQIKLDPLT 1030 Query: 836 AVVIR 840 A +++ Sbjct: 1031 AAIVQ 1035 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 12,826,460,081 Number of extensions: 558635212 Number of successful extensions: 1094610 Number of sequences better than 1.0e-05: 6654 Number of HSP's gapped: 1076559 Number of HSP's successfully gapped: 7362 Length of query: 2165 Length of database: 3,060,696,203 Length adjustment: 152 Effective length of query: 2013 Effective length of database: 1,628,701,923 Effective search space: 3278576970999 Effective search space used: 3278576970999 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 140 (58.5 bits)