BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0013.p (389 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3P9.1 B3K3P9_9BACI Pullulanase, extracellular 387 e-106 Q9EZZ4.1 Q9EZZ4_BACST Amylopullulanase 162 4e-38 Q45643.1 Q45643_9BACI Alpha-amylase-pullulanase 162 5e-38 A6N9J4.1 A6N9J4_BACST High-molecular-mass amylase 155 9e-36 B7GKY4.1 B7GKY4_ANOFW Alpha-amylase/pullulanase 154 2e-35 >B3K3P9.1 B3K3P9_9BACI Pullulanase, extracellular Length = 2528 Score = 387 bits (995), Expect = e-106, Method: Compositional matrix adjust. Identities = 194/376 (51%), Positives = 256/376 (68%), Gaps = 9/376 (2%) Query: 1 MIVVLVCSYFSFYPGSV-VNAAASKVVLVGSLQSELSTEAEPAGDWDPAASVTEMTYVDN 59 MIV L+ S P + N+A S+V LVG+LQSEL +GDWDPAA+ T M + N Sbjct: 15 MIVSLIFSMLGIPPSTADANSAPSQVTLVGTLQSELGH----SGDWDPAAAATTMNDMGN 70 Query: 60 GLYKFTGRLPAGVYEYKIALNGSWEESYGYGNYTNSAGVNKDGNIQISLAAETSVTFYYN 119 G Y+FTG LPAG YEYKIA++G W+E+YG +Y+N +G NKDGNI I+LA ++ VTFYYN Sbjct: 71 GEYRFTGSLPAGSYEYKIAIDGKWDENYGSAHYSNPSGANKDGNILITLAQDSQVTFYYN 130 Query: 120 DLTKKIADSTYYTPVEADKLPRLTGTLQTELGDARDSSPADAKATLSDPDLDGVYERSAE 179 T +IADSTYY P+EADKLPR+ G+ Q+ +G+A D SPADA+ + D D D VY +A+ Sbjct: 131 HGTHRIADSTYYAPIEADKLPRVIGSFQSGIGEAADWSPADARLIMQDSDYDHVYTVTAD 190 Query: 180 LPAGEYAYRVYVPGDTPDTDMFYPGGEQKLNLPAASKVTFTYNAQDHGVKASFTXXXXXX 239 + G++ Y++ + D +P + L LP KVTF+Y+A +HGV A FT Sbjct: 191 VYEGDHEYQIALGSDAASP--VHPANREALQLPQDVKVTFSYDAANHGVAAHFTVPADPG 248 Query: 240 XXXXXXEGQLRIHYSRTAGDYAGLGLWMWDDVAAPSAGWPSGATPFPEGQTDAYGAYVDV 299 LR+HY+R +Y +GLW++DDVA+PSA WP+GATPFP GQ DAYGA+VD+ Sbjct: 249 TPVPA--NHLRVHYNRADANYGSVGLWIFDDVASPSANWPAGATPFPGGQQDAYGAFVDI 306 Query: 300 PLKAGAKKVSFLVVNRSTEAKDGGNKLFMINTPQTNEVWVKEGSDTVTPYEPVVLPADTV 359 PLK GA+KV +VV+R + KDGG+K + +P NEVW+KEGSDTV +EPV LPADTV Sbjct: 307 PLKDGAQKVGIVVVDRISGNKDGGDKKLTLASPDMNEVWLKEGSDTVYSFEPVDLPADTV 366 Query: 360 RVHYARADSNWSQYGF 375 R+HY R D N+ YG Sbjct: 367 RIHYQRQDQNYDPYGL 382 Score = 108 bits (269), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 104/353 (29%), Positives = 155/353 (43%), Gaps = 59/353 (16%) Query: 27 LVGSLQSELSTEAEPAGDWDPAASVTEMTYVD-NGLYKFTGRLPAGVYEYKIALNGSWEE 85 ++GS QS + A DW PA + M D + +Y T + G +EY+IAL Sbjct: 153 VIGSFQSGIG----EAADWSPADARLIMQDSDYDHVYTVTADVYEGDHEYQIALGSD--- 205 Query: 86 SYGYGNYTNSAGVNKDGNIQISLAAETSVTFYYNDLTKKIADSTYYTPVEADKLPRLTGT 145 ++ V+ + L + VTF Y+ +A ++T P Sbjct: 206 --------AASPVHPANREALQLPQDVKVTFSYDAANHGVA--AHFTVPADPGTPVPANH 255 Query: 146 LQTELGDARDSSPADAK-ATLSDPDLDGVYERSAELPAGEYAYRVYVPGDTPD-----TD 199 L+ + ADA ++ D V SA PAG + PG D D Sbjct: 256 LRVHY------NRADANYGSVGLWIFDDVASPSANWPAGATPF----PGGQQDAYGAFVD 305 Query: 200 MFYPGGEQKLNLPAASKVTFTYNAQDHGVKASFTXXXXXXXXXXXXEGQ----------- 248 + G QK+ + +++ + D T EG Sbjct: 306 IPLKDGAQKVGIVVVDRISGNKDGGDK----KLTLASPDMNEVWLKEGSDTVYSFEPVDL 361 Query: 249 ----LRIHYSRTAGDYAGLGLWMW-DDVAAPSAGWPSGATPFPEGQTDAYGAYVDVPLKA 303 +RIHY R +Y GLW+W DDVA PS WP+GAT FP GQTD YGAYVD+P+K Sbjct: 362 PADTVRIHYQRQDQNYDPYGLWLWGDDVAVPSKDWPTGATMFPSGQTDRYGAYVDIPMKP 421 Query: 304 GAKKVSFLVVN--RSTEAKDGGNKLFMINTPQTNEVWVKEGSDTV--TPYEPV 352 GA+K++FLV++ + KDGG+K F + + ++++EG +TV +PY V Sbjct: 422 GAQKINFLVLDPAKGDAGKDGGDKGFTL-VDRYQHLFIQEGDNTVYTSPYGEV 473 >Q9EZZ4.1 Q9EZZ4_BACST Amylopullulanase Length = 2018 Score = 162 bits (411), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 93/203 (45%), Positives = 126/203 (62%), Gaps = 17/203 (8%) Query: 25 VVLVGSLQSELSTEAEPAGDWDPAASVTEMTYVDNGLYKFTGRLPAGVYEYKIALNGSWE 84 V LVG+LQ+EL E E WDPAA+ T M + NGLY FTG LPAG YEYKIA+NGSW+ Sbjct: 140 VTLVGNLQNELGHEKE----WDPAATATVMKHEGNGLYTFTGVLPAGTYEYKIAINGSWD 195 Query: 85 ESYGYGNYTNSAGVNKDGNIQISLAAETSVTFYYNDLTKKIADSTYYTPVEADKLPRLTG 144 E+YG AG GNI +L ET VTFYY+D T IADST+YTP+ +K PRL G Sbjct: 196 ENYG-------AGGRNGGNISFTLEKETEVTFYYHDSTHAIADSTWYTPIPKEKQPRLVG 248 Query: 145 TLQTELGDARDS---SPADAKATLSDPDLDGVYERSAELPAGEYAYRVYVPGDTPDTDMF 201 T+ +G ++ +P + A L D + D +Y +A +P G Y ++V + G++ D + Sbjct: 249 TILPAIGYEKEVDGWTPETSSAFLVDNNFDFIYTFTARVPKGNYEFKVTL-GNSWDEN-- 305 Query: 202 YPGGEQKLNLPAASKVTFTYNAQ 224 YP KLN+ + +TF +NA+ Sbjct: 306 YPQDNAKLNVLEETTITFFFNAK 328 Score = 56.2 bits (134), Expect = 5e-06, Method: Compositional matrix adjust. Identities = 38/114 (33%), Positives = 60/114 (52%), Gaps = 20/114 (17%) Query: 27 LVGSLQSELSTEAEPAGDWDPAASVTEMTYVDNG---LYKFTGRLPAGVYEYKIALNGSW 83 LVG++ + E E G W P S + VDN +Y FT R+P G YE+K+ L SW Sbjct: 246 LVGTILPAIGYEKEVDG-WTPETSSAFL--VDNNFDFIYTFTARVPKGNYEFKVTLGNSW 302 Query: 84 EESYGYGNYTNSAGVNKDGNIQISLAAETSVTFYYNDLTKKIADSTYYTPVEAD 137 +E+Y N ++++ ET++TF++N TK++ T Y+P +D Sbjct: 303 DENY------------PQDNAKLNVLEETTITFFFNAKTKEL--YTDYSPTGSD 342 >Q45643.1 Q45643_9BACI Alpha-amylase-pullulanase Length = 2032 Score = 162 bits (410), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 96/204 (47%), Positives = 124/204 (60%), Gaps = 18/204 (8%) Query: 25 VVLVGSLQSELSTEAEPAGDWDPAASVTEMTYVDNGLYKFTGRLPAGVYEYKIALNGSWE 84 V LVG+LQ EL E E WDP A+ T M + NGLY FTG LPAG YEYKIA+NGSW+ Sbjct: 135 VTLVGNLQKELGHEKE----WDPTATATVMKHEGNGLYTFTGTLPAGTYEYKIAINGSWD 190 Query: 85 ESYGYGNYTNSAGVNKDGNIQISLAAETSVTFYYNDLTKKIADSTYYTPVEADKLPRLTG 144 E+YG AG GNI +L ET VTFYY+D T IADST+YTP+ +K PRL G Sbjct: 191 ENYG-------AGGRNGGNISFTLEKETEVTFYYHDSTHAIADSTWYTPIPKEKQPRLVG 243 Query: 145 TLQTELGDARDS---SPADAKATLSDPDLDGVYERSAELPAGEYAYRVYVPGDTPDTDMF 201 T+ +G ++ +P + A +D D D VY SA +P G Y Y+V V G++ D + Sbjct: 244 TILPAIGYEKEVDGWTPETSTAFFTDDDFDSVYTFSARVPKGTYEYKV-VLGNSWDEN-- 300 Query: 202 YPGGEQ-KLNLPAASKVTFTYNAQ 224 YP KLN+ + +TF +NA+ Sbjct: 301 YPQDNNAKLNVLEETTITFFFNAK 324 Score = 61.2 bits (147), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 38/112 (33%), Positives = 61/112 (54%), Gaps = 15/112 (13%) Query: 27 LVGSLQSELSTEAEPAGDWDPAASVTEMTYVD-NGLYKFTGRLPAGVYEYKIALNGSWEE 85 LVG++ + E E G W P S T D + +Y F+ R+P G YEYK+ L SW+E Sbjct: 241 LVGTILPAIGYEKEVDG-WTPETSTAFFTDDDFDSVYTFSARVPKGTYEYKVVLGNSWDE 299 Query: 86 SYGYGNYTNSAGVNKDGNIQISLAAETSVTFYYNDLTKKIADSTYYTPVEAD 137 +Y +D N ++++ ET++TF++N TK++ T Y+P +D Sbjct: 300 NYP-----------QDNNAKLNVLEETTITFFFNAKTKEL--YTDYSPTGSD 338 >A6N9J4.1 A6N9J4_BACST High-molecular-mass amylase Length = 1650 Score = 155 bits (391), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 88/203 (43%), Positives = 119/203 (58%), Gaps = 17/203 (8%) Query: 25 VVLVGSLQSELSTEAEPAGDWDPAASVTEMTYVDNGLYKFTGRLPAGVYEYKIALNGSWE 84 + LVG+LQ EL AE WDP A T M NGLY FTG LPAG YEYKIA+NGSW+ Sbjct: 58 ITLVGNLQDELGHTAE----WDPGALATRMHDEGNGLYTFTGTLPAGTYEYKIAVNGSWD 113 Query: 85 ESYGYGNYTNSAGVNKDGNIQISLAAETSVTFYYNDLTKKIADSTYYTPVEADKLPRLTG 144 E+YG AG N+ + L +T VTFYYND T IADST+YT + +K PRL G Sbjct: 114 ENYG-------AGGRNGANLVLKLDRQTEVTFYYNDRTHAIADSTWYTAIAKEKQPRLVG 166 Query: 145 TLQTELGDARDS---SPADAKATLSDPDLDGVYERSAELPAGEYAYRVYVPGDTPDTDMF 201 T+ +G R+S +P + L+D D D VY A++P G Y Y++ + + ++ Sbjct: 167 TVLPAIGYERESNGWTPGTSTTLLTDDDFDSVYTFKAQVPKGSYEYKIVLGNNWNES--- 223 Query: 202 YPGGEQKLNLPAASKVTFTYNAQ 224 YP +LN+ + +TF +NA+ Sbjct: 224 YPQENARLNVLETTIITFFFNAK 246 >B7GKY4.1 B7GKY4_ANOFW Alpha-amylase/pullulanase Length = 1990 Score = 154 bits (388), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 87/212 (41%), Positives = 123/212 (58%), Gaps = 17/212 (8%) Query: 25 VVLVGSLQSELSTEAEPAGDWDPAASVTEMTYVDNGLYKFTGRLPAGVYEYKIALNGSWE 84 V LVG+LQ EL G+WDP A+ T MT+ +GLY FTG LPAG YEYKIA+NGSW+ Sbjct: 138 VTLVGNLQDELG----HTGEWDPKATATVMTHEGDGLYTFTGTLPAGTYEYKIAMNGSWD 193 Query: 85 ESYGYGNYTNSAGVNKDGNIQISLAAETSVTFYYNDLTKKIADSTYYTPVEADKLPRLTG 144 E+YG AG NI ++L ET VTFYY+D T IADST+YTP+ +K PRL G Sbjct: 194 ENYG-------AGGRGGANISLTLEKETEVTFYYHDRTHAIADSTWYTPIPKEKQPRLVG 246 Query: 145 TLQTELG---DARDSSPADAKATLSDPDLDGVYERSAELPAGEYAYRVYVPGDTPDTDMF 201 T+ +G + +P + A L D + D +Y +A +P G Y ++V + + Sbjct: 247 TILPAIGYETEVNGWTPETSTAFLVDDNFDSIYTFTARVPKGMYEFKVTLGNSWAEN--- 303 Query: 202 YPGGEQKLNLPAASKVTFTYNAQDHGVKASFT 233 YP KLN+ + +TF +N ++ + ++ Sbjct: 304 YPQDNAKLNVLEDATITFFFNDKEKSLYTDYS 335 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.131 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,420,518,193 Number of extensions: 108147180 Number of successful extensions: 184715 Number of sequences better than 1.0e-05: 124 Number of HSP's gapped: 184464 Number of HSP's successfully gapped: 211 Length of query: 389 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 251 Effective length of database: 1,760,596,133 Effective search space: 441909629383 Effective search space used: 441909629383 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)