BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0007.p (562 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P29094.1 O16G_BACTR Oligo-1,6-glucosidase EC=3.2.1.10 760 0.0 A6CSL5.1 A6CSL5_9BACI Oligo-1,6-glucosidase (Fragment) 759 0.0 C5D5Y7.1 C5D5Y7_9BACI Alpha amylase catalytic region 756 0.0 C3BPC6.1 C3BPC6_9BACI Oligo-1,6-glucosidase 748 0.0 C3B757.1 C3B757_BACMY Oligo-1,6-glucosidase 745 0.0 >P29094.1 O16G_BACTR Oligo-1,6-glucosidase EC=3.2.1.10 Length = 562 Score = 760 bits (1962), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/562 (65%), Positives = 443/562 (78%), Gaps = 5/562 (0%) Query: 1 MNRTFWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDD 60 M R +WKEAVVYQIYPRSF DSNGDGIGD++GII++LDYL +LGVDVVWLSPVYKSPNDD Sbjct: 1 MERVWWKEAVVYQIYPRSFYDSNGDGIGDIRGIIAKLDYLKELGVDVVWLSPVYKSPNDD 60 Query: 61 NGYDISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDN 120 NGYDISDY DIMDEFGT+ DW+ +L+ +H RGIKL+MDLV+NH+SDEH WF+ESR SKDN Sbjct: 61 NGYDISDYRDIMDEFGTMADWKTMLEEMHKRGIKLVMDLVVNHTSDEHPWFIESRKSKDN 120 Query: 121 PYRDYYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELRE 180 PYRDYYIWRPG +G+ PNNW S+FSG AWE DE TGEYYLHLFS+KQPDLNWENP++R Sbjct: 121 PYRDYYIWRPGK-NGKEPNNWESVFSGSAWEYDEMTGEYYLHLFSKKQPDLNWENPKVRR 179 Query: 181 ALYKMMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEY 240 +Y+MM FWLDKGVDG RMDVINMISKV LP MNGPRVHE+ Sbjct: 180 EVYEMMKFWLDKGVDGFRMDVINMISKVPELPDGEPQSGKKYASGSRYY--MNGPRVHEF 237 Query: 241 LQEMNRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDVDSGPGGKWNV 300 LQEMNR+VLSKY++MTVGETPG T ++ ILYT R EL MVFQFEHMD+DSGPGGKW++ Sbjct: 238 LQEMNREVLSKYDIMTVGETPGVTPKEGILYTDPSRRELNMVFQFEHMDLDSGPGGKWDI 297 Query: 301 TPWNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLLH 360 PW+L L+ + KWQ L +GWNSLYLNNHDQPR VSRFG+D +YRV SAKMLAT LH Sbjct: 298 RPWSLADLKKTMTKWQKELEGKGWNSLYLNNHDQPRAVSRFGDDGKYRVESAKMLATFLH 357 Query: 361 TLKGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEAILKAIHIKGRD 420 ++GTPYIYQGEEIGMTNV+FP +EDY+DIE NMYKE+V G D + +++ I+ KGRD Sbjct: 358 MMQGTPYIYQGEEIGMTNVRFPSIEDYRDIETLNMYKERVEEYGEDPQEVMEKIYYKGRD 417 Query: 421 NARTPMQWDSSPNAGFTEGTPWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNHPV 480 NARTPMQWD S NAGFT GTPW+ +NP Y+EINV+ AL DP+SVF+YY+KLI+LRK H + Sbjct: 418 NARTPMQWDDSENAGFTAGTPWIPVNPNYKEINVKAALEDPNSVFHYYKKLIQLRKQHDI 477 Query: 481 MVYGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEELNAVT-ETIISNYP 539 +VYG Y+L+L + YIY YTRTL E+ +++ NF + L + + T E +ISNY Sbjct: 478 IVYGTYDLILEDDPYIYRYTRTLGNEQLIVITNFSEKTPVFRLPDHIIYKTKELLISNYD 537 Query: 540 -EEQPAMDRETLRPYEARVCRL 560 +E + LRP+EARV ++ Sbjct: 538 VDEAEELKEIRLRPWEARVYKI 559 >A6CSL5.1 A6CSL5_9BACI Oligo-1,6-glucosidase (Fragment) Length = 544 Score = 759 bits (1961), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/539 (66%), Positives = 433/539 (80%), Gaps = 4/539 (0%) Query: 1 MNRTFWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDD 60 M + +WKE+V+YQIYPRSF+DSNGDGIGD+ GIIS+LDYL +LG+DV+WLSPVY+SPNDD Sbjct: 1 MTKQWWKESVIYQIYPRSFKDSNGDGIGDINGIISKLDYLKELGIDVIWLSPVYQSPNDD 60 Query: 61 NGYDISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDN 120 NGYDISDY IMDEFGT+ DW+ LL+ +H RGIKL+MDLV+NHSSDEHAWFVESR SKDN Sbjct: 61 NGYDISDYRGIMDEFGTMADWDRLLEEMHNRGIKLIMDLVVNHSSDEHAWFVESRKSKDN 120 Query: 121 PYRDYYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELRE 180 PYRDYYIWR G DG+ PNNW S FSG AW+ DE+T EY+LHLFS+KQPDLNWENP+LR+ Sbjct: 121 PYRDYYIWREG-KDGKEPNNWESCFSGSAWQYDEQTEEYFLHLFSKKQPDLNWENPKLRD 179 Query: 181 ALYKMMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEY 240 +Y MM FWLDKG+DG RMDVIN ISKV+GLP A MNGP++H+Y Sbjct: 180 EIYDMMKFWLDKGIDGFRMDVINFISKVDGLPDAELPQGKKYAPGGKYF--MNGPKIHDY 237 Query: 241 LQEMNRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDVDSGPGGKWNV 300 LQEM+ K LS Y+VMTVGE PG VE+A LYTAEDR E+ MVFQFEH+D+DSGP GKW++ Sbjct: 238 LQEMHEKALSGYDVMTVGEMPGVDVEEAKLYTAEDRKEVNMVFQFEHVDLDSGPDGKWDL 297 Query: 301 TPWNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLLH 360 P ++ LRD KWQ GL GWNSLYLNNHDQPRMVSRFGND+EYRV SAKMLAT LH Sbjct: 298 RPMKMSALRDSFTKWQKGLEQEGWNSLYLNNHDQPRMVSRFGNDKEYRVESAKMLATFLH 357 Query: 361 TLKGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEAILKAIHIKGRD 420 TLKGTPYIYQGEE+GMTNV+F + DYKDIEI NMY+EKV G DH+ ++++I++KGRD Sbjct: 358 TLKGTPYIYQGEELGMTNVRFNSINDYKDIEILNMYREKVIENGEDHDKVMESIYVKGRD 417 Query: 421 NARTPMQWDSSPNAGFTEGTPWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNHPV 480 NARTP+QWD S + GFT GTPW+++NP Y+EIN +QA+ DP+SVF+YY+KLI LRK +P+ Sbjct: 418 NARTPIQWDDSDHGGFTTGTPWIQMNPNYKEINAKQAMEDPNSVFHYYKKLIELRKENPI 477 Query: 481 MVYGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEELNAVT-ETIISNY 538 MVYG Y+L++PEH+ IYAY R E ++ +++LNF +L EL T E ISNY Sbjct: 478 MVYGSYDLIMPEHDKIYAYVREYEDQKMIVMLNFSGDTPVFELPAELEGGTKELFISNY 536 >C5D5Y7.1 C5D5Y7_9BACI Alpha amylase catalytic region Length = 562 Score = 756 bits (1951), Expect = 0.0, Method: Compositional matrix adjust. Identities = 366/562 (65%), Positives = 443/562 (78%), Gaps = 5/562 (0%) Query: 1 MNRTFWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDD 60 M R +WKEAVVYQIYPRSF DSNGDGIGD++GII++LDYL +LGVDVVWLSPVYKSPNDD Sbjct: 1 MERAWWKEAVVYQIYPRSFYDSNGDGIGDIRGIIAKLDYLKELGVDVVWLSPVYKSPNDD 60 Query: 61 NGYDISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDN 120 NGYDISDY +IMDEFGT++DWEE+L+ +H RGIKL+MDLV+NH SDEH WF+ESR SKDN Sbjct: 61 NGYDISDYREIMDEFGTMEDWEEMLEEMHKRGIKLVMDLVVNHISDEHPWFIESRKSKDN 120 Query: 121 PYRDYYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELRE 180 PYRDYYIWRPG DG+ PNNW S FSG AWE DE T EYYLHLFS+KQPDLNWENP++R Sbjct: 121 PYRDYYIWRPGK-DGKEPNNWESNFSGSAWEYDETTEEYYLHLFSKKQPDLNWENPKVRR 179 Query: 181 ALYKMMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEY 240 +Y +M FWLDKGVDG RMDVINMISKV LP MNGPRVHE+ Sbjct: 180 EVYDIMKFWLDKGVDGFRMDVINMISKVPELPDGKPQEGKKYASGSKYF--MNGPRVHEF 237 Query: 241 LQEMNRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDVDSGPGGKWNV 300 LQEMNR+VLSKY++MTVGETPG T ++ ILYT R+EL MVFQFEH+ +DSGPGGKW++ Sbjct: 238 LQEMNREVLSKYDIMTVGETPGVTPKEGILYTDPSRHELNMVFQFEHVGLDSGPGGKWDI 297 Query: 301 TPWNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLLH 360 PW+L L+ + KWQ L +GWNSLYLNNHDQPR VSRFG+D +YRV SAKMLAT LH Sbjct: 298 RPWSLADLKKTMTKWQKELEGKGWNSLYLNNHDQPRAVSRFGDDGKYRVESAKMLATFLH 357 Query: 361 TLKGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEAILKAIHIKGRD 420 ++GTPYIYQGEEIGMTNV+FP +E Y+DIE NMYKE+V G D + +++ I+ KGRD Sbjct: 358 MMQGTPYIYQGEEIGMTNVRFPSIEYYRDIETLNMYKERVEEYGEDPQKVMEKIYYKGRD 417 Query: 421 NARTPMQWDSSPNAGFTEGTPWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNHPV 480 NARTPMQWD S NAGFT GTPW+ +NP Y+EINV++ALADP+SVF+YY+KLI+ RK H + Sbjct: 418 NARTPMQWDDSENAGFTTGTPWIPVNPNYKEINVKEALADPNSVFHYYKKLIQFRKQHDI 477 Query: 481 MVYGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEELNAVT-ETIISNYP 539 +VYG Y+L+L + YIYAYTRTL E+ +++ NF + L +++ T E +ISNY Sbjct: 478 IVYGTYDLILEDDPYIYAYTRTLGNEKLIVITNFSEKTPVFRLPDDITYKTKELLISNYD 537 Query: 540 -EEQPAMDRETLRPYEARVCRL 560 +E + LRP+EARV ++ Sbjct: 538 VDETEELKEIRLRPWEARVYKI 559 >C3BPC6.1 C3BPC6_9BACI Oligo-1,6-glucosidase Length = 558 Score = 748 bits (1931), Expect = 0.0, Method: Compositional matrix adjust. Identities = 354/561 (63%), Positives = 434/561 (77%), Gaps = 5/561 (0%) Query: 1 MNRTFWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDD 60 M + +WKE+VVYQIYPRSF DSNGDGIGDL+GIIS+LDYL LG+DV+WLSPVY+SPNDD Sbjct: 1 MEKQWWKESVVYQIYPRSFMDSNGDGIGDLRGIISKLDYLQNLGIDVIWLSPVYESPNDD 60 Query: 61 NGYDISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDN 120 NGYDISDY +IMDEFGT++DW+ELL +H R +KLMMDLV+NH+SDEH WF+ESR SKDN Sbjct: 61 NGYDISDYRNIMDEFGTMNDWDELLSEMHQRDMKLMMDLVVNHTSDEHNWFIESRKSKDN 120 Query: 121 PYRDYYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELRE 180 PYRDYYIWRPG DG+ PNNW + FSGPAW+ DE T EYYLHLFS+KQPDLNW+N +R+ Sbjct: 121 PYRDYYIWRPG-KDGKEPNNWEAAFSGPAWQYDEATDEYYLHLFSKKQPDLNWDNEIVRK 179 Query: 181 ALYKMMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEY 240 +Y+MM FWL+KG+DG RMDVIN ISK EGLP+ MNGP +H Y Sbjct: 180 DVYEMMEFWLEKGIDGFRMDVINFISKTEGLPTV---KTEKEGYVSGHQYFMNGPNIHAY 236 Query: 241 LQEMNRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDVDSGPGGKWNV 300 L EMN+KVLSKY++MTVGE PG T E+A LYTAE+R ELQMVFQFEHMD+DSG GGKW+V Sbjct: 237 LHEMNQKVLSKYDIMTVGEMPGVTTEEAKLYTAEERQELQMVFQFEHMDLDSGKGGKWDV 296 Query: 301 TPWNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLLH 360 P +L L+ L KWQ L GWNSLY NNHDQPR+VSRFGND+ YR+ SAKMLAT+LH Sbjct: 297 KPCSLLTLKRNLTKWQKALEQTGWNSLYWNNHDQPRVVSRFGNDKTYRIESAKMLATVLH 356 Query: 361 TLKGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEAILKAIHIKGRD 420 +KGTPYIYQGEEIGMTNV F +++Y+DIE NMY+EKV G D E ++++I+IKGRD Sbjct: 357 MMKGTPYIYQGEEIGMTNVHFDSIDEYRDIETLNMYREKVIERGEDEEKVMQSIYIKGRD 416 Query: 421 NARTPMQWDSSPNAGFTEGTPWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNHPV 480 NARTPMQWD +AGFT G PW+ LN Y+EINV QAL D +S+FYYY+KLI LRK H + Sbjct: 417 NARTPMQWDDGKHAGFTAGEPWIALNSNYKEINVTQALQDKNSIFYYYKKLIELRKKHEI 476 Query: 481 MVYGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEELN-AVTETIISNYP 539 +VYG Y+L+ EH ++AY RT + E+ L++ NF + ++ EE+N +E +I NY Sbjct: 477 IVYGTYDLIFEEHPSVFAYMRTWKDEKVLVIANFTENECVFEIPEEVNYNSSELLIHNYD 536 Query: 540 EEQPAMDRETLRPYEARVCRL 560 + + L+PYEARV +L Sbjct: 537 VQNETIKSIDLQPYEARVYKL 557 >C3B757.1 C3B757_BACMY Oligo-1,6-glucosidase Length = 559 Score = 745 bits (1924), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/561 (62%), Positives = 432/561 (77%), Gaps = 5/561 (0%) Query: 1 MNRTFWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDD 60 M + +WKE+VVYQIYPRSF DSNGDGIGDL+GIIS+LDYL LG+DV+WLSPVY+SPNDD Sbjct: 2 MEKQWWKESVVYQIYPRSFMDSNGDGIGDLRGIISKLDYLQNLGIDVIWLSPVYESPNDD 61 Query: 61 NGYDISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDN 120 NGYDISDY +IMDEFGT++DW+ELL +H R +KLMMDLV+NH+SDEH WF+ESR SKDN Sbjct: 62 NGYDISDYRNIMDEFGTMNDWDELLSEMHQRDMKLMMDLVVNHTSDEHNWFIESRKSKDN 121 Query: 121 PYRDYYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELRE 180 PYRDYYIWRPG DG+ PNNW + FSG AW+ DE T EYYLHLFS+KQPDLNW+N +R+ Sbjct: 122 PYRDYYIWRPG-KDGKEPNNWEAAFSGSAWQYDEATDEYYLHLFSKKQPDLNWDNEIVRK 180 Query: 181 ALYKMMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEY 240 +Y+MM FWL+KG+DG RMDVIN ISK EGLP+ MNGP +H Y Sbjct: 181 DVYEMMEFWLEKGIDGFRMDVINFISKTEGLPTV---KTEKEGYVSGHQYFMNGPNIHAY 237 Query: 241 LQEMNRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDVDSGPGGKWNV 300 L EMN+KVLSKY++MTVGE PG T E+A LYTAE+R ELQMVFQFEHMD+DSG GGKW+V Sbjct: 238 LHEMNQKVLSKYDIMTVGEMPGVTTEEAKLYTAEERQELQMVFQFEHMDLDSGKGGKWDV 297 Query: 301 TPWNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLLH 360 P +L L+ L KWQ L GWNSLY NNHDQPR+VSRFGND+ YR+ SAKMLAT+LH Sbjct: 298 KPCSLLTLKRNLTKWQKALEQTGWNSLYWNNHDQPRVVSRFGNDKTYRIESAKMLATVLH 357 Query: 361 TLKGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEAILKAIHIKGRD 420 +KGTPYIYQGEEIGMTNV F +++Y+DIE NMY+EKV G D E ++++I+IKGRD Sbjct: 358 MMKGTPYIYQGEEIGMTNVHFDSIDEYRDIETLNMYREKVIERGEDEEKVMQSIYIKGRD 417 Query: 421 NARTPMQWDSSPNAGFTEGTPWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNHPV 480 NARTPMQWD +AGFT G PW+ LN Y+EINV QAL D +S+FYYY+KLI LRK H + Sbjct: 418 NARTPMQWDDGKHAGFTAGEPWIALNSNYKEINVTQALQDKNSIFYYYKKLIELRKKHEI 477 Query: 481 MVYGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEELN-AVTETIISNYP 539 +VYG Y+L+ EH ++AY RT + E+ L++ NF + ++ EE+N +E +I NY Sbjct: 478 IVYGTYDLIFEEHPSVFAYMRTWKDEKVLVIANFTENECVFEMPEEVNYNSSELLIHNYD 537 Query: 540 EEQPAMDRETLRPYEARVCRL 560 + L+PYEARV +L Sbjct: 538 VQNETSKSIDLQPYEARVYKL 558 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.138 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,791,194,342 Number of extensions: 173702854 Number of successful extensions: 380471 Number of sequences better than 1.0e-05: 4172 Number of HSP's gapped: 369176 Number of HSP's successfully gapped: 4631 Length of query: 562 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 421 Effective length of database: 1,732,333,088 Effective search space: 729312230048 Effective search space used: 729312230048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)