BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0005.p (286 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBA8.1 B3KBA8_9BACI DegV family protein 352 3e-95 A6TU61.1 A6TU61_ALKMQ DegV family protein 267 1e-69 B8CY46.1 B8CY46_HALOH DegV family protein 259 1e-67 A9NFM6.1 A9NFM6_ACHLI DegV-family protein 220 1e-55 B6FWM1.1 B6FWM1_9CLOT Putative uncharacterized protein 214 8e-54 >B3KBA8.1 B3KBA8_9BACI DegV family protein Length = 285 Score = 352 bits (902), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 161/276 (58%), Positives = 212/276 (76%) Query: 9 VKIFSDSTSDLPQGWKETYDIGIIPLYVVFEDATYRDGVDITPEEVYRRVAAQGALPKTA 68 ++IF+DST DLP W + Y+IGI+PLYVVF + + RDGVDITPE++Y +V G+LPKTA Sbjct: 5 IRIFADSTCDLPPSWIDEYNIGIVPLYVVFGEQSLRDGVDITPEQLYAKVDQIGSLPKTA 64 Query: 69 APSPADFMAAFEPVIRSGGDVVYISLSSALSSTYQNALLAAVEFPEGRVHVVDSETLCGG 128 APSP+DF+AAF P I G D++++S+SS LSSTYQNAL+AA E PEGRV V DS L G Sbjct: 65 APSPSDFIAAFSPSIEQGEDILFLSISSELSSTYQNALIAAGELPEGRVTVFDSRNLSSG 124 Query: 129 IALLVMKXXXXXXXGQSAPEIAAMVKECRSQVQTEFVVDKLDYLYMGGRCSGMQNFIGRL 188 I LLVMK G S P+I ++ ++V+ EFVVD LDYLY GGRCSGMQN IG L Sbjct: 125 IGLLVMKAVHAANEGSSIPQIVELLSRTVNEVECEFVVDTLDYLYKGGRCSGMQNIIGSL 184 Query: 189 LKIRPVLRLIDGAIVPVSKIRGKKEKAIEQMLHHALEHAGTIDKELIIVAHTLAEEDAKY 248 LKIRPV++++DG ++P K+RGKKEKA++QM+ +AL +D +LI+V HT+AEE+A++ Sbjct: 185 LKIRPVIKVVDGGMIPAYKVRGKKEKALDQMIQNALAQVDKMDNDLIVVVHTMAEEEARF 244 Query: 249 LEKALREQTGSGEIAIIHAGCVIGSHCGPGTVGLMY 284 L+K L+E+TG+ +++I AGCVI SHCGP TVGLMY Sbjct: 245 LQKELQEKTGARQVSISTAGCVICSHCGPQTVGLMY 280 >A6TU61.1 A6TU61_ALKMQ DegV family protein Length = 282 Score = 267 bits (682), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 132/279 (47%), Positives = 177/279 (63%) Query: 6 MSIVKIFSDSTSDLPQGWKETYDIGIIPLYVVFEDATYRDGVDITPEEVYRRVAAQGALP 65 M+++KI +DSTSDLP E I +IPLY+ F+D TYRDG+D+TP +Y +V A ALP Sbjct: 1 MNLIKIITDSTSDLPLSLLEKEKIPVIPLYISFDDHTYRDGIDMTPAVLYDKVKATKALP 60 Query: 66 KTAAPSPADFMAAFEPVIRSGGDVVYISLSSALSSTYQNALLAAVEFPEGRVHVVDSETL 125 KTAAPSP DF FE I G D++YI LSS LSST QNA +AA EFPEGR+ V+DS L Sbjct: 61 KTAAPSPVDFRNVFESYIDQGMDILYIGLSSKLSSTIQNARIAASEFPEGRIEVIDSLNL 120 Query: 126 CGGIALLVMKXXXXXXXGQSAPEIAAMVKECRSQVQTEFVVDKLDYLYMGGRCSGMQNFI 185 GI LLV++ + E+A ++ +V T F VD LDYL+ GGRCS NF Sbjct: 121 SAGIGLLVIEALDLVKTEKPLHEVATEIRHLIPKVTTYFAVDSLDYLHKGGRCSAASNFF 180 Query: 186 GRLLKIRPVLRLIDGAIVPVSKIRGKKEKAIEQMLHHALEHAGTIDKELIIVAHTLAEED 245 G +LKIRP+L++ G I+ K+RGK+E+ I+ ML ++ + +I+ H+ +D Sbjct: 181 GSILKIRPILKVDGGKILLDQKVRGKRERLIKAMLDRIYADQEKLNPKRVIINHSFCPKD 240 Query: 246 AKYLEKALREQTGSGEIAIIHAGCVIGSHCGPGTVGLMY 284 A YL+ L + EI + AGCVI SHCGPGT ++Y Sbjct: 241 AGYLKSELEKTLDIEEILMTTAGCVISSHCGPGTFSIVY 279 >B8CY46.1 B8CY46_HALOH DegV family protein Length = 282 Score = 259 bits (663), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 125/280 (44%), Positives = 188/280 (67%) Query: 6 MSIVKIFSDSTSDLPQGWKETYDIGIIPLYVVFEDATYRDGVDITPEEVYRRVAAQGALP 65 M+ +KIF+DSTSDL E +IG++PL V F + YRDGVDIT EE+YR+V LP Sbjct: 1 MNGIKIFTDSTSDLSSDLIEENNIGVVPLNVTFGEKVYRDGVDITTEELYRKVEETDKLP 60 Query: 66 KTAAPSPADFMAAFEPVIRSGGDVVYISLSSALSSTYQNALLAAVEFPEGRVHVVDSETL 125 T A +P ++ AF+P + G ++YI LSS LSS+ QNA+ A+ F ++ ++DS L Sbjct: 61 ITGAVTPNEYYQAFKPYVEKGYKIIYIGLSSKLSSSLQNAITASRMFDNAQIEIIDSLNL 120 Query: 126 CGGIALLVMKXXXXXXXGQSAPEIAAMVKECRSQVQTEFVVDKLDYLYMGGRCSGMQNFI 185 GI LLVMK G + E+ + V+ S+V TEFV+D LDYL+ GGRCSG++ F+ Sbjct: 121 STGIGLLVMKAVDFIKDGLNFNEVVSRVRRLVSRVNTEFVIDTLDYLHKGGRCSGLERFL 180 Query: 186 GRLLKIRPVLRLIDGAIVPVSKIRGKKEKAIEQMLHHALEHAGTIDKELIIVAHTLAEED 245 G +L+I PV++++DG ++ K RGKK++ ++++L LE+ +I K+ + V H+ +++D Sbjct: 181 GSMLRIHPVVKVVDGKMILAEKTRGKKKRVVKRLLERFLENRDSIRKDRVFVTHSHSDKD 240 Query: 246 AKYLEKALREQTGSGEIAIIHAGCVIGSHCGPGTVGLMYM 285 A++L++ LRE+ E+ I AGCVI SHCGPGTVG++Y Sbjct: 241 AEFLQEKLREELNIEEVLITGAGCVISSHCGPGTVGILYF 280 >A9NFM6.1 A9NFM6_ACHLI DegV-family protein Length = 282 Score = 220 bits (560), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 111/280 (39%), Positives = 170/280 (60%) Query: 6 MSIVKIFSDSTSDLPQGWKETYDIGIIPLYVVFEDATYRDGVDITPEEVYRRVAAQGALP 65 M+ + I +DST+DL ET +I I+PLYV F D +Y+D +DIT E+Y++V G LP Sbjct: 1 MNKILITTDSTADLSPEILETRNIKIVPLYVRFNDESYKDSIDITTPELYKKVETYGFLP 60 Query: 66 KTAAPSPADFMAAFEPVIRSGGDVVYISLSSALSSTYQNALLAAVEFPEGRVHVVDSETL 125 KT A SP DF F+ + G +V+IS+ S +S+TY++ALLA V+V+DS L Sbjct: 61 KTQAASPGDFEVFFKKFLDEGYKIVHISIGSKISATYKSALLAIDLLETKDVYVIDSANL 120 Query: 126 CGGIALLVMKXXXXXXXGQSAPEIAAMVKECRSQVQTEFVVDKLDYLYMGGRCSGMQNFI 185 GIALLV+K G SA EI + + +V ++F + LDYL+ GGR SG+ F+ Sbjct: 121 SSGIALLVLKASDLKDQGLSAEEIHQEITKLVPKVSSQFAIKTLDYLHKGGRASGLSAFV 180 Query: 186 GRLLKIRPVLRLIDGAIVPVSKIRGKKEKAIEQMLHHALEHAGTIDKELIIVAHTLAEED 245 G +L+I+P++++ DG + K GK KA++ M+ + G ID + + H+LA + Sbjct: 181 GSVLRIKPIIQVNDGKLDVYKKSVGKMSKALDVMIDDLVNLNGEIDDTYVFITHSLAPDS 240 Query: 246 AKYLEKALREQTGSGEIAIIHAGCVIGSHCGPGTVGLMYM 285 +Y++ + ++ I HAGCVI SHCG GT+G++Y+ Sbjct: 241 FEYIKNRVEKEVKVKHILEGHAGCVISSHCGEGTIGILYI 280 >B6FWM1.1 B6FWM1_9CLOT Putative uncharacterized protein Length = 285 Score = 214 bits (544), Expect = 8e-54, Method: Compositional matrix adjust. Identities = 117/282 (41%), Positives = 163/282 (57%), Gaps = 3/282 (1%) Query: 6 MSIVKIFSDSTSDLPQGWKETYDIGIIPLYVVFEDATYRDGVDITPEEVYRRVAAQGALP 65 M +KIFSDST DLP +IGIIPL + F + Y++G+DIT +E+ V G LP Sbjct: 1 MEGIKIFSDSTCDLPIEEVNRMNIGIIPLTINFGEEVYKEGIDITTKEILEIVKKTGELP 60 Query: 66 KTAAPSPADFMAAFEPVIRSGGDVVYISLSSALSSTYQNALLAAVEFPEG-RVHVVDSET 124 KTA+PSPA+F AAFEP + G ++YI LSS LSST NA++A F +H++DS Sbjct: 61 KTASPSPAEFYAAFEPYVEKGDTIIYIGLSSKLSSTVANAMIAKNMFDNNENIHIIDSLN 120 Query: 125 LCGGIALLVMKXXXXXXXGQSAPEIAAMVKECRSQVQTEFVVDKLDYLYMGGRCSGMQNF 184 LCG I LV G+S + A +++ + F +DKLDYLY GGRCSGMQ Sbjct: 121 LCGAIGGLVRNTYNLLEEGRSVEDTIAEIEKVLHHYKLFFTMDKLDYLYKGGRCSGMQFI 180 Query: 185 IGRLLKIRPVLRLIDGAIVPVSKIRGKKEKAIEQMLHHALEHAGTI-DKELIIVAHTLAE 243 +G ++P++ + + K RGKK KAIE ML A I + E+ + A T +E Sbjct: 181 LGSTFGVKPIIEMTTEGLDVWKKTRGKK-KAIEMMLEEAERDKDRIYNDEIHVAAITGSE 239 Query: 244 EDAKYLEKALREQTGSGEIAIIHAGCVIGSHCGPGTVGLMYM 285 ++ + +++ L ++TG + GC I SHCG GTVG Y Sbjct: 240 KELEGIKEQLTKRTGITKFHEYSVGCTIASHCGDGTVGFGYF 281 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,616,696,159 Number of extensions: 65249354 Number of successful extensions: 189840 Number of sequences better than 1.0e-05: 1594 Number of HSP's gapped: 187006 Number of HSP's successfully gapped: 1594 Length of query: 286 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 152 Effective length of database: 1,798,280,193 Effective search space: 273338589336 Effective search space used: 273338589336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)