BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0001.p (235 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZFS0.1 C0ZFS0_BREBN Conserved hypothetical membrane protein 171 5e-41 Q9KEN5.1 Q9KEN5_BACHD BH0817 protein 150 1e-34 P42247.4 YCBO_BACSU Uncharacterized protein ycbO 147 5e-34 B1HY92.1 B1HY92_LYSSC Hypothetical ycbO protein 145 4e-33 A3IDC0.1 A3IDC0_9BACI Putative uncharacterized protein 140 1e-31 >C0ZFS0.1 C0ZFS0_BREBN Conserved hypothetical membrane protein Length = 230 Score = 171 bits (433), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 88/231 (38%), Positives = 142/231 (61%), Gaps = 2/231 (0%) Query: 4 LLKLIQLEIRKNKLTGMLKGVAIAVLMILGFMLLLTYVDAEDGSGRGEFKTYADMFEGLY 63 +L L++LE+RK K+ G ++ AIA L+I GFM+L++Y + + G F+ Y+ + + Sbjct: 1 MLNLMKLELRKFKIMGYIRSAAIATLIIFGFMMLMSY--SPELHGINVFENYSRVLSMID 58 Query: 64 XXXXXXXXXXXXXXLSKLVIDEYKNNTITLLFMYPISRKKLLGAKIIIVFLFTLLSVFIS 123 L + VIDE+ + TIT+LFMYPI R K++ AK+++V FT L + S Sbjct: 59 VFVRGTFIIFAAVLLGRFVIDEFNHKTITVLFMYPIKRHKIIAAKLLVVIAFTFLCIVAS 118 Query: 124 DILISALLIGINSFTHVIPEQLDWAGIPGELLRMGMDAIFAAGIGLIPLYIGMRKKSVPA 183 I++S L INSFTH +P ++ + ++G A+ A+ I LIPLY GMRK SVP Sbjct: 119 TIILSTLYFFINSFTHFVPGEVTQTLVLQHAAKLGFHALAASFIALIPLYFGMRKYSVPT 178 Query: 184 TIVTAVLFXXXXXXDFGGFRMSNLVGISIFLSLVGVAIAYLSIRNIEQKDI 234 TIV++++ D GGF +++++ + I L+ +G+A+ Y++IR I+Q DI Sbjct: 179 TIVSSIIIVLLTCSDSGGFSLNDVIIVPILLAAIGIAVGYMAIRKIDQIDI 229 >Q9KEN5.1 Q9KEN5_BACHD BH0817 protein Length = 230 Score = 150 bits (378), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 80/231 (34%), Positives = 136/231 (58%), Gaps = 2/231 (0%) Query: 4 LLKLIQLEIRKNKLTGMLKGVAIAVLMILGFMLLLTYVDAEDGSGRGEFKTYADMFEGLY 63 +LKLIQLE +K + +G ++G+ IA ++I F+ +T++ E G F+TY + F + Sbjct: 1 MLKLIQLEFKKVRFSGYIRGIIIANVLIALFVTGITFL--EQLEGIYAFQTYEEAFSLIN 58 Query: 64 XXXXXXXXXXXXXXLSKLVIDEYKNNTITLLFMYPISRKKLLGAKIIIVFLFTLLSVFIS 123 +++LVI+EYK+ TIT++F YPI RKKL AK+IIVF FT +++ +S Sbjct: 59 VLVQVTFVVFAAVLIARLVIEEYKHKTITVMFSYPIQRKKLFAAKLIIVFFFTFVTILLS 118 Query: 124 DILISALLIGINSFTHVIPEQLDWAGIPGELLRMGMDAIFAAGIGLIPLYIGMRKKSVPA 183 + ++ ++ ++P+QL + + + ++GI L P+Y GM KKSVP Sbjct: 119 NAFVTTAFFFLSEHFSLVPDQLSHYLLIDHAKSLLISTATSSGISLAPVYFGMLKKSVPT 178 Query: 184 TIVTAVLFXXXXXXDFGGFRMSNLVGISIFLSLVGVAIAYLSIRNIEQKDI 234 TIV+ +L + GF +S++ I + L+ +G+ +AYL+ RNIE+ D+ Sbjct: 179 TIVSGLLLIAIVTSNNNGFSLSSITIIPVTLACIGILLAYLTFRNIEEADV 229 >P42247.4 YCBO_BACSU Uncharacterized protein ycbO Length = 228 Score = 147 bits (372), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 84/229 (36%), Positives = 138/229 (60%), Gaps = 2/229 (0%) Query: 5 LKLIQLEIRKNKLTGMLKGVAIAVLMILGFMLLLTYVDAEDGSGRGEFKTYADMFEGLYX 64 + LI++E+RK K+ ++G IA ++I+GFM L++Y + DG F++ + F + Sbjct: 1 MNLIRIELRKMKMGWYIRGALIANVIIMGFMWLISYSEKADGGV--SFQSTDEAFLIIGT 58 Query: 65 XXXXXXXXXXXXXLSKLVIDEYKNNTITLLFMYPISRKKLLGAKIIIVFLFTLLSVFISD 124 + KLVI EYKN TI ++F YPISRKKLL AK++I T +++ +S+ Sbjct: 59 FVRAVFIVFGAVLIVKLVISEYKNKTILVMFTYPISRKKLLTAKLMIAGGLTFITILLSN 118 Query: 125 ILISALLIGINSFTHVIPEQLDWAGIPGELLRMGMDAIFAAGIGLIPLYIGMRKKSVPAT 184 ILI+A +NS H IP +L I + ++M + A AAG L+P++ GMR+ SVPAT Sbjct: 119 ILIAAGFFWLNSICHFIPGELTSEIISQQAVKMAVFAFGAAGTSLVPIFFGMRRHSVPAT 178 Query: 185 IVTAVLFXXXXXXDFGGFRMSNLVGISIFLSLVGVAIAYLSIRNIEQKD 233 I+++V+ GF +S++V I + L+ G+ +Y++IRN +++D Sbjct: 179 IISSVVIVMLISSTSPGFSISSVVYIPLSLAAFGLFFSYMAIRNADKQD 227 >B1HY92.1 B1HY92_LYSSC Hypothetical ycbO protein Length = 225 Score = 145 bits (365), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 85/227 (37%), Positives = 137/227 (60%), Gaps = 4/227 (1%) Query: 9 QLEIRKNKLTGMLKGVAIAVLMILGFMLLLTYVDA-EDGSGRGEFKTYADMFEGLYXXXX 67 +LE++K L+ K A ++ILGFM ++ ++ E+ G ++ + + Sbjct: 2 RLEMKKYHLSSYFKSAMFANIIILGFMFMILFISKIEEEVGIENYEMALSVIDSF---VR 58 Query: 68 XXXXXXXXXXLSKLVIDEYKNNTITLLFMYPISRKKLLGAKIIIVFLFTLLSVFISDILI 127 ++KL+I EYK TITL FMYP+SRKKL+ AK+ IV +FT + IS++LI Sbjct: 59 AVFIIFAATLIAKLIIGEYKFKTITLAFMYPVSRKKLIAAKLAIVMIFTFSVIIISNVLI 118 Query: 128 SALLIGINSFTHVIPEQLDWAGIPGELLRMGMDAIFAAGIGLIPLYIGMRKKSVPATIVT 187 + + ++ +IP+ L I + + M+AI A+GI LIPLY GMRK S+PATI++ Sbjct: 119 TTIFCIVSDKFDLIPDVLSNTLIMQHIPSVFMNAIAASGIALIPLYFGMRKYSIPATIIS 178 Query: 188 AVLFXXXXXXDFGGFRMSNLVGISIFLSLVGVAIAYLSIRNIEQKDI 234 A+L + G F +++++ I I L+++GV+IAYL+IRNIE+ D+ Sbjct: 179 AILIVSVTSSNTGNFTLNDIIIIPISLAILGVSIAYLAIRNIEKVDV 225 >A3IDC0.1 A3IDC0_9BACI Putative uncharacterized protein Length = 229 Score = 140 bits (353), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 86/231 (37%), Positives = 138/231 (59%), Gaps = 2/231 (0%) Query: 4 LLKLIQLEIRKNKLTGMLKGVAIAVLMILGFMLLLTYVDAEDGSGRGEFKTYADMFEGLY 63 +L L++LE++K L +K A +ILGFM ++ ++ +G E Y + Sbjct: 1 MLNLMRLEMKKYHLGSYVKSALFANFIILGFMFMILFISKVEGEVGIE--NYEMALSVID 58 Query: 64 XXXXXXXXXXXXXXLSKLVIDEYKNNTITLLFMYPISRKKLLGAKIIIVFLFTLLSVFIS 123 ++KL+I EYK TITL FMYPISRKKL+ AK+ IV LFT + +S Sbjct: 59 SFVRAVFIIFAATLIAKLIIGEYKFKTITLAFMYPISRKKLIAAKLAIVMLFTFSVIIVS 118 Query: 124 DILISALLIGINSFTHVIPEQLDWAGIPGELLRMGMDAIFAAGIGLIPLYIGMRKKSVPA 183 ++LI+ + ++ +IP+ L I + + M+AI A+G+ LIPLY GMRK S+PA Sbjct: 119 NVLITTIFCIVSDKFDLIPDILSNTLIMQHIPSVLMNAIAASGMALIPLYFGMRKYSIPA 178 Query: 184 TIVTAVLFXXXXXXDFGGFRMSNLVGISIFLSLVGVAIAYLSIRNIEQKDI 234 TI++++L + G F +++++ I I L+++G++IAYL+IRNIE+ D+ Sbjct: 179 TIISSILIVSVTSSNTGNFTLNDIIIIPISLAIIGISIAYLAIRNIEKVDV 229 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.147 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,139,489,530 Number of extensions: 42771481 Number of successful extensions: 150595 Number of sequences better than 1.0e-05: 185 Number of HSP's gapped: 150351 Number of HSP's successfully gapped: 185 Length of query: 235 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 104 Effective length of database: 1,826,543,238 Effective search space: 189960496752 Effective search space used: 189960496752 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)