BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0298.0006.p (287 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DCH7.1 B1DCH7_9BACL NAD-dependent epimerase/dehydratase 384 e-105 Q9F387.1 Q9F387_STRCO Putative oxidoreductase 299 2e-79 Q08YE3.1 Q08YE3_STIAU NAD-dependent epimerase/dehydratase 295 3e-78 B5HNH9.1 B5HNH9_9ACTO NAD-dependent epimerase/dehydratase 295 4e-78 Q13KI7.1 Q13KI7_BURXL Putative epimerase/dehydratase 293 1e-77 >B1DCH7.1 B1DCH7_9BACL NAD-dependent epimerase/dehydratase Length = 276 Score = 384 bits (985), Expect = e-105, Method: Compositional matrix adjust. Identities = 193/280 (68%), Positives = 224/280 (80%), Gaps = 6/280 (2%) Query: 7 MHVFVTGATGYVGSAVVRELISAGHTVTGLCRSEEKAAGLKTAGAEALYGTLDDLDILRS 66 M +F+TGATGY+G+ VVRELI GH V GL RS++ AA L AGAEA GTL+DLD L+ Sbjct: 1 MLIFLTGATGYIGTEVVRELIEGGHQVVGLTRSDKGAALLVEAGAEAHLGTLEDLDSLQR 60 Query: 67 GAAAADGVIHLAFTNDFSDFDGALALDLRAVEVMGAALEGSGKPFIITAHANGQTVDQTV 126 GAAAADGVIHLAF +DFSDF G+LA+DLRAVE MGA LEGSGKPF+ITAHANG + V Sbjct: 61 GAAAADGVIHLAFRHDFSDFAGSLAMDLRAVEAMGAVLEGSGKPFVITAHANGIESENAV 120 Query: 127 LAMAERGIRTSIVMLAPSVHGQGDKGFVPMMINIARAKGFAAYIGDGTNRWPAVHRLDAA 186 L +AERG+RTSIV LAPSVHG+ DKGFVP +I ARAKGF+AYIGDG NRWPAVHR+DAA Sbjct: 121 LGLAERGVRTSIVSLAPSVHGERDKGFVPQLIQTARAKGFSAYIGDGVNRWPAVHRIDAA 180 Query: 187 VLYRLALESAPAGSRLLGAGDEGISLREIAAVIGRQLNVPAVSIPPEEAAAHFGFLGTIA 246 L+RLA+E+APAGSRL G GDEGI+ R+IA VIG+ LNVP SI EEAAAHFGFLG +A Sbjct: 181 SLFRLAVEAAPAGSRLDGVGDEGIAFRDIATVIGQHLNVPVRSISREEAAAHFGFLGMLA 240 Query: 247 SFDITGLYNTTQASLATRELLGWKPVQPGLIADLEEGHYF 286 DI ++S T+ELLGWKP PGL+ DLE+GHYF Sbjct: 241 GLDI------PRSSEQTQELLGWKPTHPGLLEDLEQGHYF 274 >Q9F387.1 Q9F387_STRCO Putative oxidoreductase Length = 306 Score = 299 bits (765), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 163/314 (51%), Positives = 205/314 (65%), Gaps = 41/314 (13%) Query: 7 MHVFVTGATGYVGSAVVRELISAGHTVTGLCRSEEKAAGLKTAGAEALYGTLDDLDILRS 66 M VFVTGA+G++GSAVV ELI AGH V GL RS+ A L AGA + GTLDDLD+LR Sbjct: 1 MRVFVTGASGWIGSAVVPELIRAGHQVVGLARSDASAEALTAAGAGVVRGTLDDLDVLRR 60 Query: 67 GAAAADGVIHLAFTNDFS---DFDGALALDLRAVEVMGAALEGSGKPFIITA-------- 115 AA +DGV+HLAF +D + DF GA D RAVEV+G AL GS +PF I + Sbjct: 61 AAADSDGVVHLAFKHDIAFSGDFKGAGEADRRAVEVIGEALAGSDRPFTIASGTPAIPGH 120 Query: 116 ---------------------HANGQTVDQTVLAMAERGIRTSIVMLAPSVHGQGDKGFV 154 H T + TV +++ RG+R+S+V LAP+ HG+GD GFV Sbjct: 121 VATEHDGHDADRAAAARGDAQHIRAVTAELTV-SLSARGVRSSVVRLAPTNHGEGDNGFV 179 Query: 155 PMMINIARAKGFAAYIGDGTNRWPAVHRLDAAVLYRLALESAPAGSRLLGAGDEGISLRE 214 P +I IAR KG + YIGDGTNRW A HRLD+A L+RLALE APAGS L +EG+ +R+ Sbjct: 180 PALIGIARGKGVSGYIGDGTNRWSAAHRLDSARLFRLALEKAPAGSTLHAVAEEGLPVRD 239 Query: 215 IAAVIGRQLNVPAVSIPPEEAAAHFGFL-GTIASFDITGLYNTTQASLATRELLGWKPVQ 273 +A V+GRQL +P VS+P EA AHFG+L G +A+ N +S+ TRELLGW+PVQ Sbjct: 240 LAEVVGRQLGLPVVSVPAAEAEAHFGWLAGPLAA-------NRAASSVLTRELLGWEPVQ 292 Query: 274 PGLIADLEEGHYFS 287 PGL+ DLE+GHYF Sbjct: 293 PGLVEDLEQGHYFK 306 >Q08YE3.1 Q08YE3_STIAU NAD-dependent epimerase/dehydratase Length = 310 Score = 295 bits (756), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 167/305 (54%), Positives = 202/305 (66%), Gaps = 31/305 (10%) Query: 7 MHVFVTGATGYVGSAVVRELISAGHTVTGLCRSEEKAAGLKTAGAEALYGTLDDLDILRS 66 M VFVTGA+G++GSA+V EL++AGH V GL RS+ AA L +AG E G LDDLD LR Sbjct: 1 MRVFVTGASGHIGSALVPELLAAGHKVIGLARSDTSAAALSSAGVEVRRGDLDDLDGLRQ 60 Query: 67 GAAAADGVIHLAFTNDFS---DFDGALALDLRAVEVMGAALEGSGKPFIITA-------- 115 AAAADGVIHLAF +D + D+ GA A + RAVE +GAALEGSGKPF+ TA Sbjct: 61 AAAAADGVIHLAFKHDVAFTGDYAGAAAAEFRAVEAIGAALEGSGKPFVSTAGTLALALG 120 Query: 116 -----------HANGQTVD--QTVLAMAERGIRTSIVMLAPSVHGQGDK-GFVPMMINIA 161 G +D V+A+ +RG+R+S++ LAP+VH D GFVP +I +A Sbjct: 121 GSGRMGTEDDAREGGPRIDSENAVIALGKRGVRSSVIRLAPTVHSSLDHHGFVPSLITMA 180 Query: 162 RAKGFAAYIGDGTNRWPAVHRLDAAVLYRLALESAPAGSRLLGAGDEGISLREIAAVIGR 221 R GFAAY+GDG NRWP VH LDA LYRLALE+APAGSRL GA +EGI R+IA IGR Sbjct: 181 RKNGFAAYVGDGANRWPTVHTLDATRLYRLALETAPAGSRLHGAAEEGIPFRDIAEAIGR 240 Query: 222 QLNVPAVSIPPEEAAAHFGFLGTIASFDITGLYNTTQASLATRELLGWKPVQPGLIADLE 281 L +PAVSI E+A H GFL A D N T +S+ TRELL W+P PGL+ADL Sbjct: 241 GLGLPAVSISAEDAGKHLGFLAMFAQLD-----NPT-SSVRTRELLRWQPTHPGLLADLA 294 Query: 282 EGHYF 286 E HYF Sbjct: 295 ERHYF 299 >B5HNH9.1 B5HNH9_9ACTO NAD-dependent epimerase/dehydratase Length = 298 Score = 295 bits (754), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 162/303 (53%), Positives = 197/303 (65%), Gaps = 29/303 (9%) Query: 7 MHVFVTGATGYVGSAVVRELISAGHTVTGLCRSEEKAAGLKTAGAEALYGTLDDLDILRS 66 M VFVTGATG+VGSAV REL+ AGH V GL RS+ LK AGAE G L DLD LR Sbjct: 1 MRVFVTGATGFVGSAVTRELLHAGHEVVGLARSDRSVEALKAAGAEVHRGDLTDLDSLRR 60 Query: 67 GAAAADGVIHLAFTNDFSDFDGALALDLRAVEVMGAALEGSGKPFIITA---HANGQT-- 121 GA AADGV HLAF +DF++ + DL+A+E +G LEG+GKPF++T+ A G+ Sbjct: 61 GAEAADGVAHLAFIHDFTNLTASGEADLKAIEAVGEVLEGTGKPFVVTSGLLTAPGRVGT 120 Query: 122 ----------------VDQTVLAMAERGIRTSIVMLAPSVHGQGDKGFVPMMINIARAKG 165 + LA+AERG+ S++ L PSVHG+GD GFVPM I+ AR KG Sbjct: 121 EEDPADPGSPGHHRLASEVATLALAERGVSASVLRLPPSVHGEGDLGFVPMFIDHARDKG 180 Query: 166 FAAYIGDGTNRWPAVHRLDAAVLYRLALESAPAGSRLLGAGDEGISLREIAAVIGRQLNV 225 +A +GDG+N W AVHRLDAA L+RLALE+APAG RL GDEG+ EIA IGR L+V Sbjct: 181 VSAMVGDGSNHWAAVHRLDAARLFRLALEAAPAGVRLHAIGDEGVPFHEIAETIGRGLDV 240 Query: 226 PAVSIPPEEAAAHFGFLGTIASFDITGLYNTTQASLA-TRELLGWKPVQPGLIADLEEGH 284 P S+ PEEA HFG+LG A+ D+ +AS A TRELLGW+P LI DLE GH Sbjct: 241 PVASVAPEEATDHFGWLGRFAAIDM-------RASAARTRELLGWRPEHAALIPDLEAGH 293 Query: 285 YFS 287 YF Sbjct: 294 YFK 296 >Q13KI7.1 Q13KI7_BURXL Putative epimerase/dehydratase Length = 297 Score = 293 bits (750), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 157/302 (51%), Positives = 195/302 (64%), Gaps = 27/302 (8%) Query: 7 MHVFVTGATGYVGSAVVRELISAGHTVTGLCRSEEKAAGLKTAGAEALYGTLDDLDILRS 66 M VFVTGA+G+VGSAVV ELI+AGHTV GL RSE A L AGAE YG+L+D L++ Sbjct: 1 MRVFVTGASGFVGSAVVAELIAAGHTVLGLARSEASAGSLAEAGAEVHYGSLEDTGSLKA 60 Query: 67 GAAAADGVIHLAFTNDFSDFDGALALDLRAVEVMGAALEGSGKPFIIT------------ 114 GAAAADGVIH AF +DFS F LD RA+E +GAAL GS +P ++T Sbjct: 61 GAAAADGVIHTAFNHDFSRFVENCELDRRAIEALGAALAGSERPLLVTSGVALLAPGRLA 120 Query: 115 ---------AHANGQTVDQTVLAMAERGIRTSIVMLAPSVHGQGDKGFVPMMINIARAKG 165 A A + + T L++A RG+R S+V L PSVHG GD GFVP +I +AR KG Sbjct: 121 TEDDAPVADASAYPRVSEATALSLAVRGVRASVVRLPPSVHGDGDHGFVPHLIGVAREKG 180 Query: 166 FAAYIGDGTNRWPAVHRLDAAVLYRLALESAPAGSRLLGAGDEGISLREIAAVIGRQLNV 225 +AY+G+G NRWPAVHR DAA +YRLALE AGSR +EG+ ++IA+VIGR+LNV Sbjct: 181 VSAYVGEGQNRWPAVHRFDAARVYRLALERGAAGSRYHAIAEEGVPFQQIASVIGRRLNV 240 Query: 226 PAVSIPPEEAAAHFGFLGTIASFDITGLYNTTQASLATRELLGWKPVQPGLIADLEEGHY 285 P V+ E+AA HFG+ A D +S+ TR LL W+P QPGLIADL+ Y Sbjct: 241 PVVTQSVEQAAGHFGWFALFAGID------APTSSVQTRALLNWEPEQPGLIADLDRPRY 294 Query: 286 FS 287 F Sbjct: 295 FQ 296 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,705,651,147 Number of extensions: 72456269 Number of successful extensions: 236090 Number of sequences better than 1.0e-05: 263 Number of HSP's gapped: 236176 Number of HSP's successfully gapped: 271 Length of query: 287 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 153 Effective length of database: 1,798,280,193 Effective search space: 275136869529 Effective search space used: 275136869529 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)