BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0294.0010.p (284 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1YEL1.1 B1YEL1_EXIS2 Fructose-1,6-bisphosphate aldolase, class ... 520 e-146 B3KKD8.1 B3KKD8_9BACI Fructose-1,6-bisphosphate aldolase, class ... 501 e-140 A7GV85.1 A7GV85_BACCN Fructose-1,6-bisphosphate aldolase, class ... 464 e-129 B7GMI0.1 B7GMI0_ANOFW Fructose-1,6-bisphosphate aldolase 461 e-128 A1YZ23.1 A1YZ23_9BACI Fructose-1,6-bisphosphate aldolase 459 e-127 >B1YEL1.1 B1YEL1_EXIS2 Fructose-1,6-bisphosphate aldolase, class II EC=4.1.2.13 Length = 284 Score = 520 bits (1338), Expect = e-146, Method: Compositional matrix adjust. Identities = 249/284 (87%), Positives = 268/284 (94%) Query: 1 MPLVSMTDMLNKALQGKYAVGQYNINNLEWTQAILGAAEEEKSPVILGVSEGAARHMGGF 60 MPLVSMTDMLNKAL+GKYAVGQ+NINNLEWTQA+LGAA+EE+SPVILGVSEGAARHMGGF Sbjct: 1 MPLVSMTDMLNKALEGKYAVGQFNINNLEWTQAVLGAAQEEQSPVILGVSEGAARHMGGF 60 Query: 61 YTVVKMVEGLIHDMKITVPVAIHLDHGSSFDKCKEAIDAGFTSVMIDGSHHPIDENIEMT 120 YTVVKMVEGL+HDMK+TVPVAIHLDHGSS +KCKEAIDAGFTSVMID SH PID NIE T Sbjct: 61 YTVVKMVEGLVHDMKVTVPVAIHLDHGSSVEKCKEAIDAGFTSVMIDDSHSPIDTNIETT 120 Query: 121 KKVVDYAHAKGVSVEAEVGTVGGQEDDVIGGIQYADLNECVRIVKETGIDTLAPALGSVH 180 K VV+YAH+KGVSVEAEVGTVGGQEDDVIG + YA L++CVRIVKETGIDTLAPALGSVH Sbjct: 121 KAVVEYAHSKGVSVEAEVGTVGGQEDDVIGDVMYAKLDDCVRIVKETGIDTLAPALGSVH 180 Query: 181 GPYHGEPNLGFKEMEEIRDAVKLPLVLHGGTGIPEHDIKKSISLGTSKINVNTENQIAFA 240 GPY GEPNLGF EMEEIR+A LPLVLHGGTGIP HDI+K+ISLGTSKINVNTENQI+FA Sbjct: 181 GPYKGEPNLGFTEMEEIRNATNLPLVLHGGTGIPTHDIEKAISLGTSKINVNTENQISFA 240 Query: 241 KVVREVLAAKPDAYDPRTFIAPGREAIKQTVIGKIREFGSSNKA 284 KVVREVLA KP+AYDPR FIAPGREAIKQTVIGK+REFGSSNKA Sbjct: 241 KVVREVLAEKPEAYDPRVFIAPGREAIKQTVIGKMREFGSSNKA 284 >B3KKD8.1 B3KKD8_9BACI Fructose-1,6-bisphosphate aldolase, class II EC=4.1.2.13 Length = 284 Score = 501 bits (1291), Expect = e-140, Method: Compositional matrix adjust. Identities = 242/284 (85%), Positives = 264/284 (92%) Query: 1 MPLVSMTDMLNKALQGKYAVGQYNINNLEWTQAILGAAEEEKSPVILGVSEGAARHMGGF 60 MPLVSMTDMLNKAL+GKYAVGQYNINNLEWT AIL AAE+EKSPVILGVSEGAAR+MGGF Sbjct: 1 MPLVSMTDMLNKALEGKYAVGQYNINNLEWTYAILEAAEQEKSPVILGVSEGAARYMGGF 60 Query: 61 YTVVKMVEGLIHDMKITVPVAIHLDHGSSFDKCKEAIDAGFTSVMIDGSHHPIDENIEMT 120 TVV MV+GL+ D+K+TVPVAIHLDHGSS +KCKEAIDAGFTSVMID SH PI+ NI T Sbjct: 61 STVVHMVKGLMEDLKVTVPVAIHLDHGSSVEKCKEAIDAGFTSVMIDDSHSPIETNIATT 120 Query: 121 KKVVDYAHAKGVSVEAEVGTVGGQEDDVIGGIQYADLNECVRIVKETGIDTLAPALGSVH 180 K+VV+YAHAKGVSVEAEVG VGGQEDDVIG + YA L++CVRIV ETGIDTLAPALGSVH Sbjct: 121 KQVVEYAHAKGVSVEAEVGMVGGQEDDVIGDVMYAKLDDCVRIVNETGIDTLAPALGSVH 180 Query: 181 GPYHGEPNLGFKEMEEIRDAVKLPLVLHGGTGIPEHDIKKSISLGTSKINVNTENQIAFA 240 GPY GEPNLGFKEMEEIRDAVKLPLVLHGGTGIP HDIKK+ISLGTSKINVNTENQI+F Sbjct: 181 GPYKGEPNLGFKEMEEIRDAVKLPLVLHGGTGIPTHDIKKAISLGTSKINVNTENQISFT 240 Query: 241 KVVREVLAAKPDAYDPRTFIAPGREAIKQTVIGKIREFGSSNKA 284 KVVREVLAAKP+AYDPRTF+ PGREAIK+TV+GKIREFGS+N+A Sbjct: 241 KVVREVLAAKPEAYDPRTFLKPGREAIKETVVGKIREFGSNNQA 284 >A7GV85.1 A7GV85_BACCN Fructose-1,6-bisphosphate aldolase, class II EC=4.1.2.13 Length = 285 Score = 464 bits (1194), Expect = e-129, Method: Compositional matrix adjust. Identities = 228/285 (80%), Positives = 247/285 (86%), Gaps = 1/285 (0%) Query: 1 MPLVSMTDMLNKALQGKYAVGQYNINNLEWTQAILGAAEEEKSPVILGVSEGAARHMGGF 60 MPLVSM +MLNKAL+GKYAVGQ+NINNLEWTQAIL AAEEEKSPVILGVSEGAARHM GF Sbjct: 1 MPLVSMKEMLNKALEGKYAVGQFNINNLEWTQAILAAAEEEKSPVILGVSEGAARHMTGF 60 Query: 61 YTVVKMVEGLIHDMKITVPVAIHLDHGSSFDKCKEAIDAGFTSVMIDGSHHPIDENIEMT 120 TVV MV+ LI +M ITVPVAIHLDHGSSF+KCKEAIDAGFTSVMID SHHP +EN+E T Sbjct: 61 KTVVAMVKALIEEMNITVPVAIHLDHGSSFEKCKEAIDAGFTSVMIDASHHPFEENVETT 120 Query: 121 KKVVDYAHAKGVSVEAEVGTVGGQEDDVIG-GIQYADLNECVRIVKETGIDTLAPALGSV 179 KKVV+YAHA+ VSVEAE+GTVGGQEDDVI GI YAD EC +V+ TGID LAPALGSV Sbjct: 121 KKVVEYAHARNVSVEAELGTVGGQEDDVIADGIIYADPEECKHLVEATGIDCLAPALGSV 180 Query: 180 HGPYHGEPNLGFKEMEEIRDAVKLPLVLHGGTGIPEHDIKKSISLGTSKINVNTENQIAF 239 HGPY GEPNLGFKEME++RD +PLVLHGGTGIP DIKK+ISLGTSKINVNTENQIAF Sbjct: 181 HGPYKGEPNLGFKEMEQVRDLTGVPLVLHGGTGIPTADIKKAISLGTSKINVNTENQIAF 240 Query: 240 AKVVREVLAAKPDAYDPRTFIAPGREAIKQTVIGKIREFGSSNKA 284 K VREVL + YDPR FI PGREAIK TVIGKIREFGS+ KA Sbjct: 241 TKAVREVLNKDQEVYDPRKFIGPGREAIKATVIGKIREFGSNGKA 285 >B7GMI0.1 B7GMI0_ANOFW Fructose-1,6-bisphosphate aldolase Length = 295 Score = 461 bits (1187), Expect = e-128, Method: Compositional matrix adjust. Identities = 223/285 (78%), Positives = 249/285 (87%), Gaps = 1/285 (0%) Query: 1 MPLVSMTDMLNKALQGKYAVGQYNINNLEWTQAILGAAEEEKSPVILGVSEGAARHMGGF 60 MPLVSMT+ML +AL GKYAVGQ+NINNLEWTQAIL AAEEEKSPVILGVSEGAAR+MGGF Sbjct: 10 MPLVSMTEMLKQALAGKYAVGQFNINNLEWTQAILAAAEEEKSPVILGVSEGAARYMGGF 69 Query: 61 YTVVKMVEGLIHDMKITVPVAIHLDHGSSFDKCKEAIDAGFTSVMIDGSHHPIDENIEMT 120 TVV MV+GL+ DM ITVPVAIHLDHGSSF+KCK AIDAGFTSVMID SHHP +EN+E+T Sbjct: 70 KTVVNMVKGLMEDMNITVPVAIHLDHGSSFEKCKAAIDAGFTSVMIDASHHPFEENVEIT 129 Query: 121 KKVVDYAHAKGVSVEAEVGTVGGQEDDVIG-GIQYADLNECVRIVKETGIDTLAPALGSV 179 KVV+YAHA+GVSVEAE+GTVGGQEDDV+ GI YA+ +EC +VK TGID LAPALGSV Sbjct: 130 SKVVEYAHARGVSVEAELGTVGGQEDDVVAEGIIYANPDECEELVKRTGIDCLAPALGSV 189 Query: 180 HGPYHGEPNLGFKEMEEIRDAVKLPLVLHGGTGIPEHDIKKSISLGTSKINVNTENQIAF 239 HGPY GEP LGFKEMEEIRD +PLVLHGGTGIP I+++ISLGTSKINVNTENQ+AF Sbjct: 190 HGPYKGEPKLGFKEMEEIRDRTGVPLVLHGGTGIPTEQIQRAISLGTSKINVNTENQMAF 249 Query: 240 AKVVREVLAAKPDAYDPRTFIAPGREAIKQTVIGKIREFGSSNKA 284 KVVREVLA YDPR F+ PGR+AIK+TVIGK+REFGSS KA Sbjct: 250 TKVVREVLAKDEKVYDPRKFLGPGRDAIKETVIGKMREFGSSGKA 294 >A1YZ23.1 A1YZ23_9BACI Fructose-1,6-bisphosphate aldolase Length = 286 Score = 459 bits (1182), Expect = e-127, Method: Compositional matrix adjust. Identities = 222/285 (77%), Positives = 249/285 (87%), Gaps = 1/285 (0%) Query: 1 MPLVSMTDMLNKALQGKYAVGQYNINNLEWTQAILGAAEEEKSPVILGVSEGAARHMGGF 60 MPLVSMT+ML +AL GKYAVGQ+NINNLEWTQAIL AAEEEKSPVILGVSEGAAR+MGGF Sbjct: 1 MPLVSMTEMLKQALAGKYAVGQFNINNLEWTQAILAAAEEEKSPVILGVSEGAARYMGGF 60 Query: 61 YTVVKMVEGLIHDMKITVPVAIHLDHGSSFDKCKEAIDAGFTSVMIDGSHHPIDENIEMT 120 TVV MV+GL+ DM ITVPVAIH+DHGSSF+KCK AIDAGFTSVMID SHHP +EN+E+T Sbjct: 61 KTVVNMVKGLMEDMNITVPVAIHIDHGSSFEKCKAAIDAGFTSVMIDASHHPFEENVEIT 120 Query: 121 KKVVDYAHAKGVSVEAEVGTVGGQEDDVIG-GIQYADLNECVRIVKETGIDTLAPALGSV 179 KVV+YAHA+GVSVEAE+GTVGGQEDDV+ GI YA+ +EC +VK TGID LAPALGSV Sbjct: 121 SKVVEYAHARGVSVEAELGTVGGQEDDVVAEGIIYANPDECEELVKRTGIDCLAPALGSV 180 Query: 180 HGPYHGEPNLGFKEMEEIRDAVKLPLVLHGGTGIPEHDIKKSISLGTSKINVNTENQIAF 239 HGPY GEP LGFKEMEEIRD +PLVLHGGTGIP I+++ISLGTSKINVNTENQ+AF Sbjct: 181 HGPYKGEPKLGFKEMEEIRDRTGVPLVLHGGTGIPTEQIQRAISLGTSKINVNTENQMAF 240 Query: 240 AKVVREVLAAKPDAYDPRTFIAPGREAIKQTVIGKIREFGSSNKA 284 KVVREVLA YDPR F+ PGR+AIK+TVIGK+REFGSS KA Sbjct: 241 TKVVREVLAKDEKVYDPRKFLGPGRDAIKETVIGKMREFGSSGKA 285 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,722,610,264 Number of extensions: 72262331 Number of successful extensions: 149585 Number of sequences better than 1.0e-05: 2289 Number of HSP's gapped: 143248 Number of HSP's successfully gapped: 2292 Length of query: 284 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 150 Effective length of database: 1,798,280,193 Effective search space: 269742028950 Effective search space used: 269742028950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 130 (54.7 bits)