BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0291.0007.p (257 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJZ5.1 B3KJZ5_9BACI Glutamate racemase EC=5.1.1.3 389 e-106 B1D7H2.1 B1D7H2_9BACL Glutamate racemase EC=5.1.1.3 316 2e-84 B7GH10.1 B7GH10_ANOFW Glutamate racemase 264 6e-69 Q5KWI3.1 MURI_GEOKA Glutamate racemase EC=5.1.1.3 263 1e-68 C5D5M9.1 C5D5M9_9BACI Glutamate racemase EC=5.1.1.3 262 2e-68 >B3KJZ5.1 B3KJZ5_9BACI Glutamate racemase EC=5.1.1.3 Length = 268 Score = 389 bits (999), Expect = e-106, Method: Compositional matrix adjust. Identities = 189/257 (73%), Positives = 210/257 (81%) Query: 1 VGGLTVVKEVMRQLPREKIIYFGDTARAPYGPRSTEEVKMFTEQIVDYLIQFNPKMIVIA 60 VGGLTV KEVMRQLPREKIIYFGDTARAPYGPR+ EEV++FTEQIVDYLIQ++PKMIVIA Sbjct: 12 VGGLTVAKEVMRQLPREKIIYFGDTARAPYGPRTPEEVRLFTEQIVDYLIQYDPKMIVIA 71 Query: 61 CNTATAAALDYISAKVSIPVIGVIHPGARAAISAXXXXXXXXXXXXXXXXSGAYTAALKQ 120 CNTATAAALDYIS+KV +PVIGVIHPGARAAISA SGAYTAALKQ Sbjct: 72 CNTATAAALDYISSKVPVPVIGVIHPGARAAISATKTGHVGVIGTVGTIGSGAYTAALKQ 131 Query: 121 LSPFVQVVSQACPALVPFVEQGMFRSEESHIAVAESLNGIKYEPIDTLILGCTHYPFLVE 180 LSP++ V SQACP LVP VE G+FRS++S V SL GIK ID LILGCTHYPFL+ Sbjct: 132 LSPYIGVESQACPELVPLVEHGLFRSKDSLQVVERSLRGIKDTSIDCLILGCTHYPFLMN 191 Query: 181 PIGKVMGPGVKLISSADETAREISTILYDKGKLASGDESPIHQFFCSGDAEMFQRIARDW 240 I VMGP VKLISSADETARE+STILYDKG+LAS E P+HQFFC+GD E+FQ IA +W Sbjct: 192 TIQTVMGPNVKLISSADETAREVSTILYDKGQLASSMEGPVHQFFCTGDPEIFQSIATEW 251 Query: 241 LGEQIKRTPVVWQVSSL 257 LGE IKRTPVVWQ++ L Sbjct: 252 LGEHIKRTPVVWQITKL 268 >B1D7H2.1 B1D7H2_9BACL Glutamate racemase EC=5.1.1.3 Length = 271 Score = 316 bits (809), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 154/257 (59%), Positives = 190/257 (73%) Query: 1 VGGLTVVKEVMRQLPREKIIYFGDTARAPYGPRSTEEVKMFTEQIVDYLIQFNPKMIVIA 60 VGGLTV +EVMRQLPREKI+YFGDTARAPYGPR + V FT +IVDYLIQF PKMIVIA Sbjct: 14 VGGLTVAREVMRQLPREKIVYFGDTARAPYGPRDPKIVTRFTREIVDYLIQFRPKMIVIA 73 Query: 61 CNTATAAALDYISAKVSIPVIGVIHPGARAAISAXXXXXXXXXXXXXXXXSGAYTAALKQ 120 CNTATA AL+ I A V IPV+GVI PGARAA+ SGAY AL + Sbjct: 74 CNTATAFALEEIRAIVDIPVVGVIKPGARAALGRTITGCIGVIGTEGTIKSGAYEHALLE 133 Query: 121 LSPFVQVVSQACPALVPFVEQGMFRSEESHIAVAESLNGIKYEPIDTLILGCTHYPFLVE 180 +SP V V+S+ACP VP VE+G FRS +++ + S+ ++ P+D+LILGCTHYPFL + Sbjct: 134 ISPDVHVISEACPLFVPLVEKGNFRSLQTYETIRSSIGHLRKFPMDSLILGCTHYPFLAD 193 Query: 181 PIGKVMGPGVKLISSADETAREISTILYDKGKLASGDESPIHQFFCSGDAEMFQRIARDW 240 I +V+GP + LISSADETARE+S IL D+G L+ G+ P+HQFFCSG+ +MF+ IA+ W Sbjct: 194 VIQEVVGPDINLISSADETAREVSDILQDQGMLSRGEILPVHQFFCSGEPQMFKSIAQQW 253 Query: 241 LGEQIKRTPVVWQVSSL 257 LGEQI+ TPVVWQV + Sbjct: 254 LGEQIELTPVVWQVPQI 270 >B7GH10.1 B7GH10_ANOFW Glutamate racemase Length = 278 Score = 264 bits (674), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 131/251 (52%), Positives = 164/251 (65%) Query: 1 VGGLTVVKEVMRQLPREKIIYFGDTARAPYGPRSTEEVKMFTEQIVDYLIQFNPKMIVIA 60 VGGLTV KE+MRQLP+E+I+Y GDTAR PYGPR EE++ FT ++ +YL+ ++ KM+VIA Sbjct: 25 VGGLTVAKEIMRQLPKEQIVYLGDTARCPYGPRPREEIRQFTWEMTNYLLGYDIKMLVIA 84 Query: 61 CNTATAAALDYISAKVSIPVIGVIHPGARAAISAXXXXXXXXXXXXXXXXSGAYTAALKQ 120 CNTATA AL+ I ++IPVIGV+HPGAR A+ SGAY ALK Sbjct: 85 CNTATAVALEEIRHTLNIPVIGVVHPGARTALKITKNYHIGVIGTIGTVKSGAYEQALKS 144 Query: 121 LSPFVQVVSQACPALVPFVEQGMFRSEESHIAVAESLNGIKYEPIDTLILGCTHYPFLVE 180 ++ V++ S ACP VP VE G F EE+ VAESL K PIDTLILGCTHYP L Sbjct: 145 INSDVKIESLACPKFVPLVESGQFEGEEAKQIVAESLEPFKDRPIDTLILGCTHYPLLSP 204 Query: 181 PIGKVMGPGVKLISSADETAREISTILYDKGKLASGDESPIHQFFCSGDAEMFQRIARDW 240 I + MG VKLI S DETARE+STIL+ G L +G++ H FF +G E FQ+IA W Sbjct: 205 LIQQYMGEHVKLICSGDETAREVSTILHHSGLLYTGNKRREHIFFTTGSKETFQQIASKW 264 Query: 241 LGEQIKRTPVV 251 G I+ + Sbjct: 265 FGHPIEHVETI 275 >Q5KWI3.1 MURI_GEOKA Glutamate racemase EC=5.1.1.3 Length = 264 Score = 263 bits (671), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 134/245 (54%), Positives = 163/245 (66%) Query: 1 VGGLTVVKEVMRQLPREKIIYFGDTARAPYGPRSTEEVKMFTEQIVDYLIQFNPKMIVIA 60 VGGLTV KE+MRQLP+E+IIY GDTAR PYGPR EEV+ FT Q++DYL Q+ KM+VIA Sbjct: 12 VGGLTVAKEIMRQLPKEQIIYLGDTARCPYGPRPVEEVRRFTWQMIDYLRQYPLKMLVIA 71 Query: 61 CNTATAAALDYISAKVSIPVIGVIHPGARAAISAXXXXXXXXXXXXXXXXSGAYTAALKQ 120 CNTATA ALD + AK+ IPV+GVIHPGARAA+ A S AY AL+ Sbjct: 72 CNTATAVALDDVRAKLDIPVLGVIHPGARAALKATRRGHIGVIGTIGTIRSRAYEKALQS 131 Query: 121 LSPFVQVVSQACPALVPFVEQGMFRSEESHIAVAESLNGIKYEPIDTLILGCTHYPFLVE 180 ++P VQV S ACP VP VE G F +E+ VAESL ++ PID LILGCTHYP L Sbjct: 132 INPRVQVESLACPKFVPLVESGDFEGQEAMAIVAESLAPLRPLPIDVLILGCTHYPLLAP 191 Query: 181 PIGKVMGPGVKLISSADETAREISTILYDKGKLASGDESPIHQFFCSGDAEMFQRIARDW 240 I MG VKLI S ETARE+S IL+ L +G+ P H FF +G E+F++I+ W Sbjct: 192 LIRTYMGKRVKLICSGGETAREVSAILHHSQLLYTGEREPEHLFFTTGPKELFEKISGKW 251 Query: 241 LGEQI 245 G+ I Sbjct: 252 FGKPI 256 >C5D5M9.1 C5D5M9_9BACI Glutamate racemase EC=5.1.1.3 Length = 264 Score = 262 bits (670), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 129/251 (51%), Positives = 162/251 (64%) Query: 1 VGGLTVVKEVMRQLPREKIIYFGDTARAPYGPRSTEEVKMFTEQIVDYLIQFNPKMIVIA 60 VGGLTV KE+MRQLP+E+I+Y GDTAR PYGPR EE++ FT ++ +YL+++N KM+VIA Sbjct: 12 VGGLTVAKEIMRQLPKERIVYLGDTARCPYGPRPKEEIRQFTWEMTNYLLRYNIKMLVIA 71 Query: 61 CNTATAAALDYISAKVSIPVIGVIHPGARAAISAXXXXXXXXXXXXXXXXSGAYTAALKQ 120 CNTATA LD I ++ IPV+GV+HPGARAA+ A SGAY ALK Sbjct: 72 CNTATAVVLDEIREQLDIPVLGVVHPGARAALKATKNGHIGVIGTIGTVKSGAYEKALKS 131 Query: 121 LSPFVQVVSQACPALVPFVEQGMFRSEESHIAVAESLNGIKYEPIDTLILGCTHYPFLVE 180 ++ V+V S ACP VP VE G F EE+ VAESL K +D LILGCTHYP L Sbjct: 132 INHRVRVESLACPKFVPLVESGNFEGEEARKIVAESLEPFKSSNMDVLILGCTHYPLLSP 191 Query: 181 PIGKVMGPGVKLISSADETAREISTILYDKGKLASGDESPIHQFFCSGDAEMFQRIARDW 240 I + MG VKLI S DETARE+S IL+ L +G H FF +G E+FQ+IA W Sbjct: 192 LIKEYMGKRVKLICSGDETAREVSAILHHSHLLYTGQREAEHLFFTTGSKELFQKIASKW 251 Query: 241 LGEQIKRTPVV 251 G+ I+ + Sbjct: 252 FGKPIENVQTI 262 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,355,573,127 Number of extensions: 48833782 Number of successful extensions: 129220 Number of sequences better than 1.0e-05: 1501 Number of HSP's gapped: 127070 Number of HSP's successfully gapped: 1504 Length of query: 257 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 124 Effective length of database: 1,807,701,208 Effective search space: 224154949792 Effective search space used: 224154949792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)