BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0078.p (435 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG16.1 B3KG16_9BACI RNA modification enzyme, MiaB family EC=1.... 733 0.0 B1DFR2.1 B1DFR2_9BACL RNA modification enzyme, MiaB family EC=1.... 698 0.0 C0ZB52.1 C0ZB52_BREBN Putative uncharacterized protein 593 e-167 B7GKD2.1 B7GKD2_ANOFW 2-methylthioadenine synthetase 568 e-160 Q9KD68.1 Q9KD68_BACHD BH1351 protein 564 e-159 >B3KG16.1 B3KG16_9BACI RNA modification enzyme, MiaB family EC=1.3.1.74 Length = 447 Score = 733 bits (1893), Expect = 0.0, Method: Compositional matrix adjust. Identities = 346/435 (79%), Positives = 386/435 (88%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGPADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEAI 60 VNFYDTEAIWQ FKNEGY+QVDFE ADVYLINTC VTNTGDKKSRQMIRRAVRRNPEAI Sbjct: 13 VNFYDTEAIWQYFKNEGYDQVDFEQTADVYLINTCHVTNTGDKKSRQMIRRAVRRNPEAI 72 Query: 61 VAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREFE 120 VAVTGCYAQTSP EILDIPGVDLVIGNQDRE IM + +I+++RQP+NAVRNIMKTR FE Sbjct: 73 VAVTGCYAQTSPAEILDIPGVDLVIGNQDREKIMDFIHDIEKTRQPINAVRNIMKTRVFE 132 Query: 121 ELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKEF 180 E+DVP FA+RTRAF+KIQDGCNNFCTFCIIPWSRGLSRSRDPKSI+ QA+QLV AGYKE Sbjct: 133 EMDVPDFAERTRAFLKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIIKQAHQLVGAGYKEI 192 Query: 181 VLTGIHTGGYGDDLENYRLADLLWELDQVDGLERVRISSIEASQIDGKLLDVLNRSSKMC 240 VLTGIHTGGYGDDLE+YRL+DLLW+LD+VDGLER+RISSIEASQID K+LDVLNRSSKMC Sbjct: 193 VLTGIHTGGYGDDLEDYRLSDLLWDLDRVDGLERIRISSIEASQIDEKMLDVLNRSSKMC 252 Query: 241 RHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMFR 300 RHLHIPLQAG + VLK MRRKYTTEE+Y KM LIRQAMPDVGITTDVIVGFPGET+EMFR Sbjct: 253 RHLHIPLQAGDDTVLKRMRRKYTTEEFYNKMGLIRQAMPDVGITTDVIVGFPGETDEMFR 312 Query: 301 AGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYARR 360 GYD MKA+ +SEMHVFPYSKRTGTPAARM +QVDE++KNARV LIDLSE++QLAYA R Sbjct: 313 NGYDLMKAIQFSEMHVFPYSKRTGTPAARMEDQVDEEVKNARVHDLIDLSEQLQLAYAER 372 Query: 361 FVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDSLQGELCQVKITEAGVNECRG 420 FVG+ ++VI ER K AP G+SDNYLQ+ FNG L+G+LC+VK+T+AGVNEC G Sbjct: 373 FVGQILNVIPEREYKGAPKGGKMQGYSDNYLQLVFNGSPDLEGKLCRVKVTKAGVNECEG 432 Query: 421 ELVSVNRSGLRQIVQ 435 EL+SV GL+ Q Sbjct: 433 ELLSVMDHGLKAAAQ 447 >B1DFR2.1 B1DFR2_9BACL RNA modification enzyme, MiaB family EC=1.3.1.74 Length = 448 Score = 698 bits (1802), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/425 (76%), Positives = 370/425 (87%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGPADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEAI 60 VNFYDTEAIWQLFKNEGYEQVDFE ADVYLINTCTVTNTGDKKSRQ+IRRAVRRNP+A+ Sbjct: 13 VNFYDTEAIWQLFKNEGYEQVDFESTADVYLINTCTVTNTGDKKSRQIIRRAVRRNPDAV 72 Query: 61 VAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREFE 120 +AVTGCYAQTSP EI+ IPGVDLVIG QDRE IMT V I + RQPVNAVRNIMKTREFE Sbjct: 73 IAVTGCYAQTSPAEIMAIPGVDLVIGTQDREKIMTFVNQIHDDRQPVNAVRNIMKTREFE 132 Query: 121 ELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKEF 180 ELDVP F +RTRAF+KIQ+GCNNFCTFCIIPWSRGLSRSRDPKS++ QA QLV +GYKE Sbjct: 133 ELDVPDFNERTRAFLKIQEGCNNFCTFCIIPWSRGLSRSRDPKSVLEQAKQLVASGYKEV 192 Query: 181 VLTGIHTGGYGDDLENYRLADLLWELDQVDGLERVRISSIEASQIDGKLLDVLNRSSKMC 240 VLTGIHTGGYGDD+ENYRL DLLW+LD+++GLER+RISSIEASQID ++DVLNRS+KMC Sbjct: 193 VLTGIHTGGYGDDMENYRLVDLLWDLDKIEGLERIRISSIEASQIDDAMIDVLNRSTKMC 252 Query: 241 RHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMFR 300 RHLHIPLQAG VLK MRRKYTT+E+ AK++ IR+AMP V ITTDVIVGFPGETEEMF Sbjct: 253 RHLHIPLQAGDTSVLKRMRRKYTTDEFAAKLKRIREAMPGVAITTDVIVGFPGETEEMFE 312 Query: 301 AGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYARR 360 GY FM+ V +SEMHVFPYSKRTGTPAARM +QVD+++KN RV +LIDLSE+MQLAYA + Sbjct: 313 NGYKFMEEVGFSEMHVFPYSKRTGTPAARMDDQVDDEVKNERVHKLIDLSEKMQLAYAEQ 372 Query: 361 FVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDSLQGELCQVKITEAGVNECRG 420 VG + VI ER K APG + G+SDNY+Q+ F G ++L G+LC+VKITEAGVNECR Sbjct: 373 HVGAVLDVIPERDYKGAPGTGLVMGYSDNYIQIVFEGTEALVGKLCRVKITEAGVNECRA 432 Query: 421 ELVSV 425 +LV V Sbjct: 433 QLVRV 437 >C0ZB52.1 C0ZB52_BREBN Putative uncharacterized protein Length = 452 Score = 593 bits (1529), Expect = e-167, Method: Compositional matrix adjust. Identities = 277/426 (65%), Positives = 344/426 (80%), Gaps = 1/426 (0%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGP-ADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEA 59 VN Y+TEAIWQLFK +GYE+VDFE ADVY+INTCTVTNTGDKKSRQ+IRRA+RRNPEA Sbjct: 13 VNSYETEAIWQLFKADGYERVDFEQEDADVYVINTCTVTNTGDKKSRQVIRRAIRRNPEA 72 Query: 60 IVAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREF 119 IVAVTGCYAQTSP EI IPGVD+V+G Q RE ++ +V I+ R P+NAV NIMK R+F Sbjct: 73 IVAVTGCYAQTSPSEIAQIPGVDIVVGTQGREKLIEYVDQIRAERTPINAVGNIMKARDF 132 Query: 120 EELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKE 179 EELDVP F DRTRA +KIQ+GCNNFCTFCIIPW+RGL RSR P+S+V QA +LVEAGY E Sbjct: 133 EELDVPNFTDRTRASLKIQEGCNNFCTFCIIPWARGLMRSRKPESVVEQAQKLVEAGYLE 192 Query: 180 FVLTGIHTGGYGDDLENYRLADLLWELDQVDGLERVRISSIEASQIDGKLLDVLNRSSKM 239 VLTGIHTGGYG+DLE+Y LA LL +L QV+GL+R+RISSIEASQI ++++V+N S ++ Sbjct: 193 IVLTGIHTGGYGEDLEDYNLAKLLIDLHQVEGLKRIRISSIEASQITDEVIEVINNSDRV 252 Query: 240 CRHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMF 299 RHLH+PLQAG +EVLK MRRKYTT E+Y +M +R+A+P ITTDVIVGFPGETEE F Sbjct: 253 VRHLHVPLQAGDDEVLKRMRRKYTTAEFYERMVKVREALPGAAITTDVIVGFPGETEEQF 312 Query: 300 RAGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYAR 359 GY+FMK + ++EMHVFPYS RTGTPAARM +Q+ E+ KN RV +L++L++E+ LAY++ Sbjct: 313 WNGYEFMKKIGFAEMHVFPYSMRTGTPAARMTDQIPEEEKNERVAKLLELNQELTLAYSK 372 Query: 360 RFVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDSLQGELCQVKITEAGVNECR 419 +FVG + VI ER K AP + G+SDNYL V F GD+S+ G++C+V++ E G C+ Sbjct: 373 KFVGDVLEVIPERPFKEAPDSGLLMGYSDNYLNVVFPGDESMIGKICKVRLDEPGSEYCK 432 Query: 420 GELVSV 425 G V V Sbjct: 433 GTFVRV 438 >B7GKD2.1 B7GKD2_ANOFW 2-methylthioadenine synthetase Length = 467 Score = 568 bits (1463), Expect = e-160, Method: Compositional matrix adjust. Identities = 266/426 (62%), Positives = 335/426 (78%), Gaps = 1/426 (0%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGPADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEAI 60 VN Y+TEAIWQLFK GYE+ DFE ADVY+INTCTVTNTGDKKSRQ+IRRAVRRNP+A+ Sbjct: 30 VNHYETEAIWQLFKQAGYERKDFESHADVYVINTCTVTNTGDKKSRQVIRRAVRRNPDAV 89 Query: 61 VAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREFE 120 V VTGCYAQTSP E++ IPGVD+VIG QDR I+ +++ ++ RQP+N VRNIMKTR +E Sbjct: 90 VCVTGCYAQTSPAEVMAIPGVDIVIGTQDRGKILEYIEQFKQQRQPINGVRNIMKTRVYE 149 Query: 121 ELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKEF 180 ELDVP F DRTRA +KIQ+GCNNFCTFCIIPW+RGL RSRDPK ++ QA QLV+AGYKE Sbjct: 150 ELDVPAFTDRTRASLKIQEGCNNFCTFCIIPWARGLMRSRDPKEVIRQAQQLVDAGYKEI 209 Query: 181 VLTGIHTGGYGDDLENYRLADLLWELD-QVDGLERVRISSIEASQIDGKLLDVLNRSSKM 239 VLTGIHTGGYG+D+++Y A LL ++D QV GL+R+RISSIEASQI +++DVL +S K+ Sbjct: 210 VLTGIHTGGYGEDMKDYNFAMLLRDMDEQVKGLKRLRISSIEASQITDEVIDVLRQSDKI 269 Query: 240 CRHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMF 299 RHLHIPLQ+G N VLK MRRKYTTE + ++ +R+ PD+ +T+DVIVGFPGETEE F Sbjct: 270 VRHLHIPLQSGSNAVLKRMRRKYTTEFFAERLARLREVFPDLAVTSDVIVGFPGETEEEF 329 Query: 300 RAGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYAR 359 Y+F++ +SE+HVFPYSKRTGTPAARM +QVDE++KN RV +LI LS+++ YA Sbjct: 330 METYEFIREQRFSELHVFPYSKRTGTPAARMPDQVDEEVKNERVHRLITLSDQLAKEYAS 389 Query: 360 RFVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDSLQGELCQVKITEAGVNECR 419 +F G+ + VI E K P + G++DNYL+V F + + G+L +VKIT+AG Sbjct: 390 KFEGQVLEVIPEELYKEDPASGLYVGYTDNYLKVKFPATEEMVGQLVKVKITKAGYPYNE 449 Query: 420 GELVSV 425 GE V V Sbjct: 450 GEFVRV 455 >Q9KD68.1 Q9KD68_BACHD BH1351 protein Length = 448 Score = 564 bits (1454), Expect = e-159, Method: Compositional matrix adjust. Identities = 258/425 (60%), Positives = 339/425 (79%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGPADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEAI 60 VN Y+TEAIWQLFK EGYE+VDFE ADVY+INTCTVTNTGDKKSRQ+IRRA+R+NP+A+ Sbjct: 13 VNHYETEAIWQLFKGEGYEKVDFEQMADVYVINTCTVTNTGDKKSRQVIRRAIRKNPDAV 72 Query: 61 VAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREFE 120 + VTGCYAQTSP E++ IPGVD+V+G QDR+ ++ +++ ++ RQP+N V NIMK+R +E Sbjct: 73 ICVTGCYAQTSPAEVMAIPGVDIVVGTQDRKKMLEYIEQYKKERQPINGVGNIMKSRVYE 132 Query: 121 ELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKEF 180 ELDVP F DRTRA +KIQ+GCNNFCTFCIIPW+RGL RSRDPK ++ QA QLV+AGYKE Sbjct: 133 ELDVPAFTDRTRASLKIQEGCNNFCTFCIIPWARGLMRSRDPKEVIKQAEQLVQAGYKEI 192 Query: 181 VLTGIHTGGYGDDLENYRLADLLWELDQVDGLERVRISSIEASQIDGKLLDVLNRSSKMC 240 VLTGIHTGGYG+DL++Y LA LL +L+QV+GL+R+RISSIEASQ+ ++++V++RS+K+ Sbjct: 193 VLTGIHTGGYGEDLKDYSLARLLEDLEQVNGLKRIRISSIEASQLTDEVIEVIDRSTKVV 252 Query: 241 RHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMFR 300 RHLHIPLQ+G + VLK MRRKYT + ++E +R+A+P + +T+DVIVGFPGETE F+ Sbjct: 253 RHLHIPLQSGSDTVLKRMRRKYTMAFFAERLERLREALPGLAVTSDVIVGFPGETEAEFQ 312 Query: 301 AGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYARR 360 YDF+ +SE+HVFPYSKRTGTPAARM +QV+ED+KN RV +LI LS+++ YA + Sbjct: 313 ETYDFIAKHKFSELHVFPYSKRTGTPAARMEDQVNEDVKNERVHRLIQLSDQLAKEYASQ 372 Query: 361 FVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDSLQGELCQVKITEAGVNECRG 420 F G+ + +I E K P + G++DNYL+V D + G+L +VKIT+AG G Sbjct: 373 FEGEVLEMIPEERDKEEPNSGLYVGYTDNYLKVKIPATDEMIGKLVKVKITKAGYPYNEG 432 Query: 421 ELVSV 425 E V V Sbjct: 433 EFVRV 437 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,512,430,074 Number of extensions: 100572705 Number of successful extensions: 269966 Number of sequences better than 1.0e-05: 3029 Number of HSP's gapped: 262412 Number of HSP's successfully gapped: 3137 Length of query: 435 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 296 Effective length of database: 1,751,175,118 Effective search space: 518347834928 Effective search space used: 518347834928 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)