BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0076.p (212 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG14.1 B3KG14_9BACI Peptidase M50 157 7e-37 C2PNB3.1 C2PNB3_BACCE Metal-dependent protease 119 1e-25 A8FIH5.1 A8FIH5_BACP2 M50 family membrane metalloprotease 119 3e-25 C2QK95.1 C2QK95_BACCE Metal-dependent protease 119 3e-25 A4ITN6.1 A4ITN6_GEOTN Membrane metalloprotease 118 3e-25 >B3KG14.1 B3KG14_9BACI Peptidase M50 Length = 230 Score = 157 bits (396), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 81/168 (48%), Positives = 101/168 (60%) Query: 1 MDSLDRILVYPLQQLPFFLITIVIAFTVHEFAHAYFANKFGDPTARLLGRMTLNPAVHXX 60 MD LD++L YPL+QLPF+LI ++IAFTVHEF+HAYFANKFGDPTA+LLGR+TLNPAVH Sbjct: 1 MDFLDQLLFYPLEQLPFYLIALIIAFTVHEFSHAYFANKFGDPTAKLLGRVTLNPAVHFD 60 Query: 61 XXXXXXXXXXXXXWARPVPVNRDNFSRPRLMGVIVSAAGPLSNFXXXXXXXXXXXXXXXT 120 WARPVPVNR+NFS PR M +IV+A GPLSN T Sbjct: 61 FLGILLLLIAGFGWARPVPVNRENFSNPRRMSIIVAAVGPLSNLVLGLVGALVYAILYQT 120 Query: 121 GVMDSIANEQLLRAVYWFFGMXXXXXXXXXXXXXXXXXXXDGYRIVED 168 GV+ + +AV+ FF + DGYR++E+ Sbjct: 121 GVIPPEGITKTDQAVHLFFNIFILLNFFLCLFNLIPLPPLDGYRVLEN 168 >C2PNB3.1 C2PNB3_BACCE Metal-dependent protease Length = 224 Score = 119 bits (299), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 67/165 (40%), Positives = 85/165 (51%), Gaps = 1/165 (0%) Query: 4 LDRILVYPLQQLPFFLITIVIAFTVHEFAHAYFANKFGDPTARLLGRMTLNPAVHXXXXX 63 +D++ YPLQQ+P + I+IA +VHEFAHAY A KFGD TA+ GR+TL+P H Sbjct: 1 MDQLFRYPLQQIPLVAMAIIIALSVHEFAHAYVAYKFGDDTAKKQGRLTLSPMAHLDPIG 60 Query: 64 XXXXXXXXXXWARPVPVNRDNFSRPRLMGVIVSAAGPLSNFXXXXXXXXXXXXXXXTGVM 123 WARPVPVN NF RPRL G++VS AGP+SN GV+ Sbjct: 61 MIAVLILGFGWARPVPVNPYNFKRPRLAGILVSIAGPISNLILSAIGLIIWYSLLTFGVL 120 Query: 124 DSIANEQLLRAVYWFFGMXXXXXXXXXXXXXXXXXXXDGYRIVED 168 DSI + + FF + DGYR+VED Sbjct: 121 DSIPFA-VADTLAQFFRIFIMLNIVLLVFNLLPIPPLDGYRVVED 164 >A8FIH5.1 A8FIH5_BACP2 M50 family membrane metalloprotease Length = 220 Score = 119 bits (297), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 68/168 (40%), Positives = 87/168 (51%), Gaps = 9/168 (5%) Query: 4 LDRILVYPLQQLPFFLITIVIAFTVHEFAHAYFANKFGDPTARLLGRMTLNPAVHXXXXX 63 L++ LVYPL+ +P+ IT+V+AFT+HE AHAY A +FGDPTA+ GR+TLNP H Sbjct: 3 LNQFLVYPLELIPYVAITLVVAFTMHELAHAYVAYRFGDPTAKNQGRLTLNPLKHLDVFG 62 Query: 64 XXXXXXXXXXWARPVPVNRDNFSRPRLMGVIVSAAGPLSNFXXXXXXXXXXXXXXXTGVM 123 WARPVPVNR +F PRL G+ VS AGPLSN ++ Sbjct: 63 TILIFVAGFGWARPVPVNRYHFKNPRLAGICVSIAGPLSNLVLAFLGCFVYV------LL 116 Query: 124 DSIANEQLLR---AVYWFFGMXXXXXXXXXXXXXXXXXXXDGYRIVED 168 E L+R V +FF + DGYRI+ED Sbjct: 117 QKFGGESLMRFEPGVDYFFRIFINLNVILFLFNLLPIPPLDGYRIIED 164 >C2QK95.1 C2QK95_BACCE Metal-dependent protease Length = 224 Score = 119 bits (297), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 65/165 (39%), Positives = 86/165 (52%), Gaps = 1/165 (0%) Query: 4 LDRILVYPLQQLPFFLITIVIAFTVHEFAHAYFANKFGDPTARLLGRMTLNPAVHXXXXX 63 +D++ YPLQ++P + I+IA +VHEFAHAY A KFGD TA+ GR+TL+P H Sbjct: 1 MDQLFRYPLQEIPLVAMAIIIALSVHEFAHAYVAYKFGDDTAKRQGRLTLSPMAHLDPIG 60 Query: 64 XXXXXXXXXXWARPVPVNRDNFSRPRLMGVIVSAAGPLSNFXXXXXXXXXXXXXXXTGVM 123 WARPVPVN NF RPRL G++VS AGP+SN GV+ Sbjct: 61 MIAVLIIGFGWARPVPVNPYNFKRPRLAGILVSIAGPISNLILSAIGLIIWYSLITFGVL 120 Query: 124 DSIANEQLLRAVYWFFGMXXXXXXXXXXXXXXXXXXXDGYRIVED 168 D+I + + + FF + DGYR+VED Sbjct: 121 DAIP-PAVGKTLSQFFQIFIGLNIVLLVFNLLPIPPLDGYRVVED 164 >A4ITN6.1 A4ITN6_GEOTN Membrane metalloprotease Length = 237 Score = 118 bits (296), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 66/168 (39%), Positives = 88/168 (52%), Gaps = 1/168 (0%) Query: 1 MDSLDRILVYPLQQLPFFLITIVIAFTVHEFAHAYFANKFGDPTARLLGRMTLNPAVHXX 60 ++ ++RIL Y L ++P+ L+T+ +AF+VHEFAHAY A KFGDPTA+ GR+TL+P H Sbjct: 13 VNQVERILPYSLSEIPYVLLTLALAFSVHEFAHAYVAYKFGDPTAKNQGRLTLSPFAHLD 72 Query: 61 XXXXXXXXXXXXXWARPVPVNRDNFSRPRLMGVIVSAAGPLSNFXXXXXXXXXXXXXXXT 120 WARPVPVNR +F PRL G++VS AGPLSN Sbjct: 73 LFGTLLIFLAGFGWARPVPVNRFHFKNPRLAGILVSVAGPLSNLLLAGLGFIVWYAMIDF 132 Query: 121 GVMDSIANEQLLRAVYWFFGMXXXXXXXXXXXXXXXXXXXDGYRIVED 168 G+M ++ N FF + DGYRI+ED Sbjct: 133 GIMRALPN-WFAAGFDMFFQIFIGLNTVLFVFNLLPFPPLDGYRIIED 179 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.144 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 879,385,674 Number of extensions: 26044123 Number of successful extensions: 37857 Number of sequences better than 1.0e-05: 398 Number of HSP's gapped: 37416 Number of HSP's successfully gapped: 398 Length of query: 212 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 82 Effective length of database: 1,835,964,253 Effective search space: 150549068746 Effective search space used: 150549068746 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)