BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0075.p (353 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG13.1 B3KG13_9BACI Ribosomal protein L11 methyltransferase 444 e-123 B1DFR5.1 B1DFR5_9BACL Ribosomal protein L11 methyltransferase 414 e-114 A3ID41.1 A3ID41_9BACI Ribosomal protein L11 methyltransferase 318 3e-85 C0ZB50.1 PRMA_BREBN Ribosomal protein L11 methyltransferase EC=2... 308 6e-82 A9VHT9.1 A9VHT9_BACWK Ribosomal protein L11 methyltransferase 307 1e-81 >B3KG13.1 B3KG13_9BACI Ribosomal protein L11 methyltransferase Length = 325 Score = 444 bits (1143), Expect = e-123, Method: Compositional matrix adjust. Identities = 228/355 (64%), Positives = 266/355 (74%), Gaps = 32/355 (9%) Query: 1 MLWHELTVHTTEEAQEMISNLLYEAGAGGVSIEESGTLNKARDTRYGELYDE--PLNDIP 58 MLW ELT+HTTEEA EMI+N L+EAGAGGVSIEESGT NK RDT G+ +DE P NDIP Sbjct: 1 MLWRELTIHTTEEAVEMITNFLHEAGAGGVSIEESGTTNKKRDTSLGQWFDEIVPFNDIP 60 Query: 59 EGEAVIKGYFAEAVSMDDIIEELAPRVAELRGFGIDPGKALISWKTVDEEDWAHAWKQYF 118 EG+AVIKGYF E V+++ I++E+ PRV EL+ FGI+ G+ KTVDE DWA AWKQYF Sbjct: 61 EGQAVIKGYFDETVTLEAILDEITPRVNELKEFGIETGEVRYEMKTVDENDWATAWKQYF 120 Query: 119 KPLRVSDRLTIKPTWEEYTPVDAAEKIIELDPGMAFGTGTHPTTALCLRALEQHIEGGEE 178 KP+RVSDRLTIKPTWEEYTP E IIELDPGMAFGTGTHPTT+LCLR LE I+GGEE Sbjct: 121 KPIRVSDRLTIKPTWEEYTPESEHESIIELDPGMAFGTGTHPTTSLCLRTLEMVIKGGEE 180 Query: 179 VIDVGTGSGILAVGAVLLGAKSVLALDLDPVAVNSARENVALNRLQDKIAVKESDXXXXX 238 VIDVGTGSGIL++GA LGA +LALDLDPVAV+SA EN LN L+D+I VKESD Sbjct: 181 VIDVGTGSGILSIGAAHLGASRILALDLDPVAVSSATENTRLNGLEDRITVKESDL---- 236 Query: 239 XXXERALDTAAGEMWPSARPGHPVDTGVDSSGLAGEAAYGVTLPVKIVVANILAEIIVLF 298 + DSS ++ GVTLPV+IVVANILAE+I+LF Sbjct: 237 -----------------------LSVLTDSS---DDSDLGVTLPVQIVVANILAEVILLF 270 Query: 299 TDDVYRALQPGGIYITSGIYKDKEEVVAKALVSSGFEIMEVSREEDWVAFTAGKR 353 DDVY+ALQPGG YI SGIYK+KEE+V KAL++SGFEI ++ RE+DWVAF A K Sbjct: 271 VDDVYQALQPGGAYIASGIYKNKEEIVEKALIASGFEIADICREDDWVAFVARKN 325 >B1DFR5.1 B1DFR5_9BACL Ribosomal protein L11 methyltransferase Length = 333 Score = 414 bits (1064), Expect = e-114, Method: Compositional matrix adjust. Identities = 214/352 (60%), Positives = 259/352 (73%), Gaps = 24/352 (6%) Query: 1 MLWHELTVHTTEEAQEMISNLLYEAGAGGVSIEESGTLNKARDTRYGELYDEPLNDIPEG 60 M WHE+T+ TTE+A EMISN L+E AGGVSIEESGTLNK RDT G+ Y++PLNDIPEG Sbjct: 1 MKWHEITISTTEQAIEMISNFLHEMDAGGVSIEESGTLNKERDTSLGQWYEKPLNDIPEG 60 Query: 61 EAVIKGYFAEAVSMDDIIEELAPRVAELRGFGIDPGKALISWKTVDEEDWAHAWKQYFKP 120 +A+IKGYF E+V M+ + ++ PR+ +L+ FGIDPG IS+K VDEEDWA AWKQYFKP Sbjct: 61 QAIIKGYFTESVDMNGLKLQIVPRIEQLKEFGIDPGTYEISFKLVDEEDWATAWKQYFKP 120 Query: 121 LRVSDRLTIKPTWEEYTPVDAAEKIIELDPGMAFGTGTHPTTALCLRALEQHIEGGEEVI 180 ++VS+RLTIKPTWEEY P A E IIELDPGMAFGTGTHPTT+LCL+ LE I+G EEVI Sbjct: 121 IKVSERLTIKPTWEEYEPA-AGENIIELDPGMAFGTGTHPTTSLCLQTLESVIKGDEEVI 179 Query: 181 DVGTGSGILAVGAVLLGAKSVLALDLDPVAVNSARENVALNRLQDKIAVKESDXXXXXXX 240 DVGTGSGILA+GA+ LGA+ VLALDLDPVAV+SA EN+ LN LQD + V ESD Sbjct: 180 DVGTGSGILAIGAMKLGARRVLALDLDPVAVSSATENIRLNGLQDDVEVLESDL------ 233 Query: 241 XERALDTAAGEMWPSARPGHPVDTGVDSSGLAGEAAYGVTLPVKIVVANILAEIIVLFTD 300 L G++ V + A VTLPV +VVANILAEII+LF D Sbjct: 234 ----LGVLGGKV-------------VHAVNERDALATSVTLPVDLVVANILAEIILLFID 276 Query: 301 DVYRALQPGGIYITSGIYKDKEEVVAKALVSSGFEIMEVSREEDWVAFTAGK 352 DV+ AL+P GIYI SGIY +KE V + L+ SGFEI++ R++DW+AF A K Sbjct: 277 DVFSALKPNGIYIASGIYMNKETDVEEGLIRSGFEIVDKRRDQDWIAFVARK 328 >A3ID41.1 A3ID41_9BACI Ribosomal protein L11 methyltransferase Length = 313 Score = 318 bits (816), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 168/352 (47%), Positives = 226/352 (64%), Gaps = 41/352 (11%) Query: 1 MLWHELTVHTTEEAQEMISNLLYEAGAGGVSIEESGTLNKARDTRYGELYDEPLNDIPEG 60 M W EL++HT EA E ISN+L+EAGA GV IE+S K R+ ++GE+Y D P+ Sbjct: 1 MKWSELSIHTKNEAVEAISNILHEAGASGVVIEDSAEFAKPREDQFGEIYALNEEDFPKD 60 Query: 61 EAVIKGYFAEAVSMDDIIEELAPRVAELRGFGIDPGKALISWKTVDEEDWAHAWKQYFKP 120 ++K Y +E+ +++ +EE+ + L F ID G+ ++S V+EEDWA AWKQY+ P Sbjct: 61 GVIVKAYLSESSFLNETVEEIKAAITNLTNFNIDIGENVVSIVEVNEEDWATAWKQYYHP 120 Query: 121 LRVSDRLTIKPTWEEYTPVDAAEKIIELDPGMAFGTGTHPTTALCLRALEQHIEGGEEVI 180 +++S+R TI PTWE+YTPV E IIELDPGMAFGTGTHPTT +CL+ LE+ I+ G+ V+ Sbjct: 121 VKISERFTIVPTWEQYTPVSTDELIIELDPGMAFGTGTHPTTVMCLQGLEKVIKEGDTVV 180 Query: 181 DVGTGSGILAVGAVLLGAKSVLALDLDPVAVNSARENVALNRLQDKIAVKESDXXXXXXX 240 D+GTGSG+L++GA LLGAK+V ALDLD VAV SA+ENVALN+++DK+AV Sbjct: 181 DIGTGSGVLSIGAALLGAKNVHALDLDEVAVRSAQENVALNKVEDKVAVFH--------- 231 Query: 241 XERALDTAAGEMWPSARPGHPVDTGVDSSGLAGEAAYGVTLPVKIVVANILAEIIVLFTD 300 G+ +DT V P +VVANILAEII+ FTD Sbjct: 232 ------------------GNLLDT--------------VKEPADVVVANILAEIIMSFTD 259 Query: 301 DVYRALQPGGIYITSGIYKDKEEVVAKALVSSGFEIMEVSREEDWVAFTAGK 352 D + ++PGG+Y+TSGI K + V AL +SGF I EV EDWVA A + Sbjct: 260 DAFTIVKPGGLYVTSGIIGAKRDDVKVALEASGFIIEEVLLMEDWVAIIARR 311 >C0ZB50.1 PRMA_BREBN Ribosomal protein L11 methyltransferase EC=2.1.1.- Length = 313 Score = 308 bits (788), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 166/354 (46%), Positives = 224/354 (63%), Gaps = 42/354 (11%) Query: 1 MLWHELTVHTTEEAQEMISNLLYEAGAGGVSIEESGTLNKARDTRYGELYDEPLNDIPEG 60 M W E+++HTT EA E +S+LLYE GA GV IE+ L + DT +GE+Y +D P Sbjct: 1 MKWSEISIHTTAEATEAVSSLLYELGANGVVIEDPEVLYREWDTPFGEIYQLSPDDFPAE 60 Query: 61 EAVIKGYF-AEAVSMDDIIEELAPRVAELRGFGIDPGKALISWKTVDEEDWAHAWKQYFK 119 +K Y ++ + D++EEL ++A+L +G+D GKA I+ V E++WAHAWK+Y+K Sbjct: 61 GVFVKAYLPVDSSELLDVVEELKEQLAQLIEYGLDIGKASIAVNDVHEDEWAHAWKKYYK 120 Query: 120 PLRVSDRLTIKPTWEEYTPVDAAEKIIELDPGMAFGTGTHPTTALCLRALEQHIEGGEEV 179 P+ VSDR+TIKP WEEY P E IIE+DPGMAFGTGTHPTT LCLRALE+++ G++V Sbjct: 121 PVHVSDRMTIKPVWEEYVPKHPDEIIIEMDPGMAFGTGTHPTTILCLRALEKYMAKGDQV 180 Query: 180 IDVGTGSGILAVGAVLLGAKSVLALDLDPVAVNSARENVALNRLQDKIAVKESDXXXXXX 239 DVGTG+ IL++ A+ LGAK VLA+DLD VAV SA+ N LN + + I V++++ Sbjct: 181 YDVGTGTAILSIAAIKLGAKDVLAMDLDEVAVRSAQANTELNGVHEHINVRQNNL----- 235 Query: 240 XXERALDTAAGEMWPSARPGHPVDTGVDSSGLAGEAAYGVTLPVKIVVANILAEIIVLFT 299 LD G+ V++VVANILAE+IV FT Sbjct: 236 -----LD-------------------------------GIEEQVEVVVANILAEVIVRFT 259 Query: 300 DDVYRALQPGGIYITSGIYKDKEEVVAKALVSSGFEIMEVSREEDWVAFTAGKR 353 DDV+R L+PGG +I+SGI +E V ALV+SG EI+E +DWVA A KR Sbjct: 260 DDVFRVLKPGGTFISSGIIAAREADVKAALVASGLEIVETIFIDDWVAIVAKKR 313 >A9VHT9.1 A9VHT9_BACWK Ribosomal protein L11 methyltransferase Length = 312 Score = 307 bits (786), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 160/350 (45%), Positives = 217/350 (62%), Gaps = 41/350 (11%) Query: 1 MLWHELTVHTTEEAQEMISNLLYEAGAGGVSIEESGTLNKARDTRYGELYDEPLNDIPEG 60 M W E+++HTTEEA E +S++L+EAGA GV+IE+ L K R +YGE+Y ++ P Sbjct: 1 MKWSEISIHTTEEAVEAVSHILHEAGASGVAIEDPAELTKERKQQYGEIYALNPDEYPSE 60 Query: 61 EAVIKGYFAEAVSMDDIIEELAPRVAELRGFGIDPGKALISWKTVDEEDWAHAWKQYFKP 120 ++K YF + S+ + + + + L F I+ GK I+ VDEEDWA AWK+Y+ P Sbjct: 61 GVLVKAYFPQTDSLHETVAGVKSSIDALPSFDIEIGKGTITINEVDEEDWATAWKKYYHP 120 Query: 121 LRVSDRLTIKPTWEEYTPVDAAEKIIELDPGMAFGTGTHPTTALCLRALEQHIEGGEEVI 180 +++SD TI PTWEEYTP EKIIELDPGMAFGTGTHPTT +C+RALE+ ++ G+ VI Sbjct: 121 VQISDTFTIVPTWEEYTPSSPNEKIIELDPGMAFGTGTHPTTTMCIRALEKTVQPGDTVI 180 Query: 181 DVGTGSGILAVGAVLLGAKSVLALDLDPVAVNSARENVALNRLQDKIAVKESDXXXXXXX 240 DVGTGSG+L++ A LGA SV A DLDPVAV SA NV LN+ D + V ++ Sbjct: 181 DVGTGSGVLSIAAAKLGAASVQAYDLDPVAVESAEMNVRLNKTDDIVNVGQNSLLE---- 236 Query: 241 XERALDTAAGEMWPSARPGHPVDTGVDSSGLAGEAAYGVTLPVKIVVANILAEIIVLFTD 300 G+ PV ++VAN+LAEII+LF + Sbjct: 237 -------------------------------------GIEGPVDLIVANLLAEIILLFPE 259 Query: 301 DVYRALQPGGIYITSGIYKDKEEVVAKALVSSGFEIMEVSREEDWVAFTA 350 D R ++PGG++ITSGI KE++++KAL ++GF I EV R EDWVA A Sbjct: 260 DAARVVKPGGLFITSGIIGAKEKIISKALETAGFSIEEVLRMEDWVAIIA 309 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,164,140,212 Number of extensions: 94183715 Number of successful extensions: 209946 Number of sequences better than 1.0e-05: 1260 Number of HSP's gapped: 208498 Number of HSP's successfully gapped: 1500 Length of query: 353 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 216 Effective length of database: 1,770,017,148 Effective search space: 382323703968 Effective search space used: 382323703968 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)