BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0057.p (343 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG06.1 B3KG06_9BACI Heat-inducible transcription repressor HrcA 495 e-138 B1DFS2.1 B1DFS2_9BACL Heat-inducible transcription repressor HrcA 388 e-106 C0ZB46.1 HRCA_BREBN Heat-inducible transcription repressor hrcA 349 2e-94 Q9KD74.1 HRCA_BACHD Heat-inducible transcription repressor hrcA 325 4e-87 A8VU79.1 A8VU79_9BACI Methyltransferase type 11 325 6e-87 >B3KG06.1 B3KG06_9BACI Heat-inducible transcription repressor HrcA Length = 344 Score = 495 bits (1274), Expect = e-138, Method: Compositional matrix adjust. Identities = 242/344 (70%), Positives = 277/344 (80%), Gaps = 1/344 (0%) Query: 1 MLTERQKMILNAIVDDYITSAEPVGSRSISKRGDVGYSPATIRNEMADLEDLGYLEQPHT 60 MLT+RQ+MILNAIVDDYI SAEPVGSRSISKRGDVGYSPATIRNEMADLE+LG+LEQPHT Sbjct: 1 MLTDRQRMILNAIVDDYIRSAEPVGSRSISKRGDVGYSPATIRNEMADLEELGFLEQPHT 60 Query: 61 SAGRIPSHKGYRYYVDHLVPWNSAESAELDTIRAFFAEKLNATEQVIQHAAMILSNMTNY 120 SAGRIPSHKGYRYYVDHL S AEL +++FFAEKLN EQ+IQH A ILS MTNY Sbjct: 61 SAGRIPSHKGYRYYVDHLTSSFSLNPAELHMMKSFFAEKLNVMEQMIQHTAGILSQMTNY 120 Query: 121 TSILLGPEVFHTSLRHFQLLPLDDRTAVAIIVTSTGQVENKTVSIPPEISLSEMEKXXXX 180 TSILLGPEVFHTSLRHFQLLPL + AVAIIVTSTGQVENK VSIP +S+SE+E+ Sbjct: 121 TSILLGPEVFHTSLRHFQLLPLHEDQAVAIIVTSTGQVENKVVSIPQGVSVSELEQVVNL 180 Query: 181 XXXXXXXXXXYKLKSQLYSELGEEMQRHISHYEELMKVLDTALESDHEQR-IYLSGATNM 239 YKLK++LYSE+ EEMQRHISHYE+LM +LD AL ++ + R +YLSG T+M Sbjct: 181 LNKKLVNVPLYKLKTRLYSEIAEEMQRHISHYEDLMSMLDIALNNEPDHRGVYLSGTTHM 240 Query: 240 LTQPEFXXXXXXXXXXXXXEETPTLLKMLAPASGGTGMQVRIGMENKHEAFANCSLITAT 299 L QPEF E+TP L+K++APA G G+QV+IG EN HEA +NCSLITAT Sbjct: 241 LNQPEFKDVDKVREIFELLEQTPALMKLMAPAPGEHGVQVKIGTENTHEAISNCSLITAT 300 Query: 300 YSLDGKALGSIGILGPTRMEYARVMGILGILSRDLTAMLAHWYK 343 YS+DGKALGSIGILGPTRMEYARV+GIL +LS DLT +L+HWYK Sbjct: 301 YSIDGKALGSIGILGPTRMEYARVIGILNVLSHDLTKLLSHWYK 344 >B1DFS2.1 B1DFS2_9BACL Heat-inducible transcription repressor HrcA Length = 308 Score = 388 bits (997), Expect = e-106, Method: Compositional matrix adjust. Identities = 180/307 (58%), Positives = 234/307 (76%), Gaps = 2/307 (0%) Query: 37 YSPATIRNEMADLEDLGYLEQPHTSAGRIPSHKGYRYYVDHLVPWNSAESAELDTIRAFF 96 +SPATIRNEMADLE+LG+LEQPHTSAGRIPS KGYRYYVDHLV N +L +FF Sbjct: 4 FSPATIRNEMADLEELGFLEQPHTSAGRIPSTKGYRYYVDHLVKLNEMNEKDLKQAHSFF 63 Query: 97 AEKLNATEQVIQHAAMILSNMTNYTSILLGPEVFHTSLRHFQLLPLDDRTAVAIIVTSTG 156 +EK+ EQVIQ AAMILSN+TNYTSILLGPE+F SL+HF L+PLD+ TAVAIIVT+TG Sbjct: 64 SEKMIQMEQVIQQAAMILSNLTNYTSILLGPEMFSNSLKHFNLVPLDESTAVAIIVTNTG 123 Query: 157 QVENKTVSIPPEISLSEMEKXXXXXXXXXXXXXXYKLKSQLYSELGEEMQRHISHYEELM 216 VEN+T+++PP I + EMEK +LKS+LY+E+G+E++R++ H EE++ Sbjct: 124 HVENRTITVPPGIRMDEMEKIVNILNAKLVGIPLVRLKSKLYAEVGQELERYVDHCEEVL 183 Query: 217 KVLDTALESDHEQRIYLSGATNMLTQPEFXXXXXXXXXXXXXEETPTLLKMLAPASGGTG 276 +LD+AL +D EQR++LSG TNMLTQPEF +ETPT++KM++ A +G Sbjct: 184 GILDSALSNDSEQRVFLSGTTNMLTQPEFKDVDKVKTILDLLDETPTVMKMISAAP--SG 241 Query: 277 MQVRIGMENKHEAFANCSLITATYSLDGKALGSIGILGPTRMEYARVMGILGILSRDLTA 336 +QVRIG EN HEA ANCSLITATY +DG++LG+IGILGPTRMEY +V+ +L ++S+D+ Sbjct: 242 IQVRIGTENDHEAIANCSLITATYQIDGQSLGTIGILGPTRMEYGKVISLLDVISKDMAH 301 Query: 337 MLAHWYK 343 +L +YK Sbjct: 302 ILGRFYK 308 >C0ZB46.1 HRCA_BREBN Heat-inducible transcription repressor hrcA Length = 341 Score = 349 bits (896), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 169/343 (49%), Positives = 237/343 (69%), Gaps = 3/343 (0%) Query: 1 MLTERQKMILNAIVDDYITSAEPVGSRSISKRGDVGYSPATIRNEMADLEDLGYLEQPHT 60 ML++RQ+MILNAIVD+YI SAEPVGSR+ISKR D+G+S ATIRNEM+DLE+LGYLEQPHT Sbjct: 1 MLSDRQQMILNAIVDNYIHSAEPVGSRTISKRDDIGFSSATIRNEMSDLEELGYLEQPHT 60 Query: 61 SAGRIPSHKGYRYYVDHLVPWNSAESAELDTIRAFFAEKLNATEQVIQHAAMILSNMTNY 120 SAGR+PS KGYR+YVD+L+ + E EL ++ FAE++ EQV+++ A ILS +TNY Sbjct: 61 SAGRVPSTKGYRFYVDNLIQPHLLEETELGKLKQLFAERILHAEQVVEYTAQILSQLTNY 120 Query: 121 TSILLGPEVFHTSLRHFQLLPLDDRTAVAIIVTSTGQVENKTVSIPPEISLSEMEKXXXX 180 T+I+LGPE+F L+H Q++PL+ AVAI+VT TG+VENK + +P I E+EK Sbjct: 121 TAIVLGPEIFEHRLKHIQIVPLNAEQAVAIVVTHTGRVENKLIDLPEGIGAGEIEKLVNL 180 Query: 181 XXXXXXXXXXYKLKSQLYSELGEEMQRHISHYEELMKVLDTALESDHEQRIYLSGATNML 240 ++L+ +LY E+ EMQRH YEE++++L+ +L + E+R+YL GAT ++ Sbjct: 181 LNAKLTDVPLWQLRQRLYQEISGEMQRHTEQYEEILQLLNNSL-TQEEERVYLRGATKIM 239 Query: 241 TQPEFXXXXXXXXXXXXXEETPTLLKMLAPASGGTGMQVRIGMENKHEAFANCSLITATY 300 QPEF E+ L+ ++ G G+ VRIG EN+ +A CS+IT +Y Sbjct: 240 NQPEFRDVDKVKDILELLEQHDQLMNVIGMQ--GDGLTVRIGQENQLDAIKQCSIITTSY 297 Query: 301 SLDGKALGSIGILGPTRMEYARVMGILGILSRDLTAMLAHWYK 343 SL GK +G +GILGPTRMEY +V+ +L L+ L+ ML ++ Sbjct: 298 SLGGKPVGMVGILGPTRMEYGKVITVLNHLAEGLSRMLTSQFE 340 >Q9KD74.1 HRCA_BACHD Heat-inducible transcription repressor hrcA Length = 343 Score = 325 bits (833), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 158/343 (46%), Positives = 228/343 (66%), Gaps = 2/343 (0%) Query: 1 MLTERQKMILNAIVDDYITSAEPVGSRSISKRGDVGYSPATIRNEMADLEDLGYLEQPHT 60 MLT+RQ +IL AIVDDYI SAEPVGSRSISKR D+ +SPATIRNEMADLE+LG+LE+PH+ Sbjct: 1 MLTDRQLLILQAIVDDYIRSAEPVGSRSISKREDITFSPATIRNEMADLEELGFLEKPHS 60 Query: 61 SAGRIPSHKGYRYYVDHLVPWNSAESAELDTIRAFFAEKLNATEQVIQHAAMILSNMTNY 120 SAGRIPS KGYRYYVDHL+ + E I F +++ EQ IQ +A ILS+MT Y Sbjct: 61 SAGRIPSQKGYRYYVDHLLMPHRLTKKERHYIHELFTKRIQEVEQAIQQSAQILSSMTRY 120 Query: 121 TSILLGPEVFHTSLRHFQLLPLDDRTAVAIIVTSTGQVENKTVSIPPEISLSEMEKXXXX 180 S++LGPE+F T L+ ++PL + V I VT TG VEN+T+ +P ++ ++EK Sbjct: 121 ISVVLGPEMFETKLKSIHMIPLTAHSVVVIFVTDTGYVENQTMHLPHSVNPVDLEKTVNI 180 Query: 181 XXXXXXXXXXYKLKSQLYSELGEEMQRHISHYEELMKVLDTALESDHEQRIYLSGATNML 240 ++L+ +L E+ + ++ H+++YE ++ +L+ E++ ++++ G TN+L Sbjct: 181 LNERLAGVSLFQLREKLNKEVADVLRFHVTNYELVLDLLEKTFEAEKSEKVFYGGKTNLL 240 Query: 241 TQPEFXXXXXXXXXXXXXEETPTLLKMLAPASGGTGMQVRIGMENKHEAFANCSLITATY 300 +QPEF E+ + +M+ S G +QVRIG EN EAF + S+ITA+Y Sbjct: 241 SQPEFRDVEKIKQILTILEQDDVMHQMI--RSSGEQIQVRIGHENNVEAFEDLSVITASY 298 Query: 301 SLDGKALGSIGILGPTRMEYARVMGILGILSRDLTAMLAHWYK 343 S+ GK +G+IGI+GPTRMEY R + ++ LS+DLT +L Y+ Sbjct: 299 SIGGKHMGTIGIVGPTRMEYRRTISVVEHLSKDLTKLLTDLYQ 341 >A8VU79.1 A8VU79_9BACI Methyltransferase type 11 Length = 342 Score = 325 bits (832), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 158/343 (46%), Positives = 221/343 (64%), Gaps = 2/343 (0%) Query: 1 MLTERQKMILNAIVDDYITSAEPVGSRSISKRGDVGYSPATIRNEMADLEDLGYLEQPHT 60 MLTERQ +IL AI+DDY++ AEPVGSRS+SKR D+ +SPATIRNEM+DLED+G+LE+ H+ Sbjct: 1 MLTERQLLILKAIIDDYVSHAEPVGSRSVSKRDDIHFSPATIRNEMSDLEDMGFLEKTHS 60 Query: 61 SAGRIPSHKGYRYYVDHLVPWNSAESAELDTIRAFFAEKLNATEQVIQHAAMILSNMTNY 120 S+GRIPSHKGYR+YVDHL+ ++ I+ + + E+V++H+A +LS +TNY Sbjct: 61 SSGRIPSHKGYRHYVDHLLSPGQLSKEDIGNIQDVLRSRFHELEEVVRHSAKLLSELTNY 120 Query: 121 TSILLGPEVFHTSLRHFQLLPLDDRTAVAIIVTSTGQVENKTVSIPPEISLSEMEKXXXX 180 TSI+LGPE+F + LR QL+P+ D+ AVAIIVT TG VEN+TV P I+ ++EK Sbjct: 121 TSIVLGPEMFESKLRQIQLIPISDKQAVAIIVTDTGHVENQTVHFPERINPDDLEKVVNI 180 Query: 181 XXXXXXXXXXYKLKSQLYSELGEEMQRHISHYEELMKVLDTALESDHEQRIYLSGATNML 240 YKL L E+ M+++++ +E ++ V +++Y SG TN+L Sbjct: 181 LNDRLRGVPLYKLNEALSKEIHAVMKQYVNRHEAMLNVFHEVFRQHAREKVYFSGKTNIL 240 Query: 241 TQPEFXXXXXXXXXXXXXEETPTLLKMLAPASGGTGMQVRIGMENKHEAFANCSLITATY 300 QPEF EE + K+ S GM +RIG EN F +C++ITATY Sbjct: 241 AQPEFNDVERVRKILNIFEEDQLVSKLF--RSDQEGMTIRIGEENHFAPFDDCTIITATY 298 Query: 301 SLDGKALGSIGILGPTRMEYARVMGILGILSRDLTAMLAHWYK 343 G+ LG++GILGPTRMEY RV+ I+ L+ DL+ L+ YK Sbjct: 299 EAGGQYLGTVGILGPTRMEYPRVISIMEYLATDLSKKLSTDYK 341 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.131 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,750,024,814 Number of extensions: 63908651 Number of successful extensions: 151844 Number of sequences better than 1.0e-05: 967 Number of HSP's gapped: 149976 Number of HSP's successfully gapped: 976 Length of query: 343 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 207 Effective length of database: 1,779,438,163 Effective search space: 368343699741 Effective search space used: 368343699741 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)