BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0056.p (179 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1HUU2.1 B1HUU2_LYSSC Short chain dehydrogenase family protein 142 2e-32 C2QTQ7.1 C2QTQ7_BACCE Short chain dehydrogenase 135 2e-30 C3C310.1 C3C310_BACTU Short chain dehydrogenase 134 3e-30 Q737G7.1 Q737G7_BACC1 Short chain dehydrogenase family protein 133 7e-30 C3H1J8.1 C3H1J8_BACTU Short chain dehydrogenase 132 1e-29 >B1HUU2.1 B1HUU2_LYSSC Short chain dehydrogenase family protein Length = 269 Score = 142 bits (357), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 57/111 (51%), Positives = 82/111 (73%) Query: 69 VGLTASLHYEAEAYGVKVSCLCPAFVDTPIFDSGEAVHMDXXXXXXXXXXXXXMTPEHFA 128 +GLT SLHYEAE +G+KVS LCP FVDTPIFD+ +A+HMD ++P+ A Sbjct: 159 IGLTTSLHYEAEEFGIKVSALCPTFVDTPIFDNAKAIHMDKSIITKQLQQQKMISPQRIA 218 Query: 129 GIAFKGIERNEELVNPMPLRRTMDVVFTLFPSLHRKVMRLVCRTTREARLS 179 IA +G+ +N+ ++ PMP+R+TMDV+FT+FP LH+ +MR+VC+ +R ARL+ Sbjct: 219 KIAIEGVHQNKPIICPMPMRKTMDVIFTIFPFLHKALMRMVCKVSRNARLT 269 Score = 60.8 bits (146), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 26/67 (38%), Positives = 45/67 (67%) Query: 4 IQRVGIVTGGASGIGKALCKELLKRRVYIVIADLNAEEGVKTVAELEGLGGACRFVQVDV 63 ++++ +VTGG SGIGKA+C+EL+ R ++++IAD+ +EG A+L +FV ++V Sbjct: 1 MKKIAVVTGGVSGIGKAICQELITRHIFVIIADIQEQEGRMLEAQLNKDAVHAKFVPLNV 60 Query: 64 ADGPAVG 70 D +V Sbjct: 61 TDYNSVA 67 >C2QTQ7.1 C2QTQ7_BACCE Short chain dehydrogenase Length = 271 Score = 135 bits (339), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 64/135 (47%), Positives = 81/135 (60%) Query: 45 TVAELEGLGGACRFVQVDVADGPAVGLTASLHYEAEAYGVKVSCLCPAFVDTPIFDSGEA 104 A GLG A VGLT SLHYEAE YGV VS LCP FVDTPIF+ A Sbjct: 137 NTASATGLGPAPLCTAYATTKHAIVGLTTSLHYEAEEYGVNVSVLCPTFVDTPIFEKSIA 196 Query: 105 VHMDXXXXXXXXXXXXXMTPEHFAGIAFKGIERNEELVNPMPLRRTMDVVFTLFPSLHRK 164 +HMD ++ + FA IA K I +N+ ++ PMP ++TMDV FTLFP LH+K Sbjct: 197 IHMDKGQIIKQLKTNKPISADKFAEIALKAIHKNKVVICPMPFKKTMDVFFTLFPYLHKK 256 Query: 165 VMRLVCRTTREARLS 179 +MR VCR R+A+++ Sbjct: 257 LMRFVCRIGRKAKMT 271 Score = 59.3 bits (142), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 29/69 (42%), Positives = 43/69 (62%), Gaps = 1/69 (1%) Query: 1 MDQIQRVGIVTGGASGIGKALCKELLKRRVYIVIADLNAEEGVKTVAELEGLGGACRFVQ 60 MD+ QRV I+TGGASGIGKAL +L + ++++IAD+N G V ++ RF Sbjct: 1 MDE-QRVAIITGGASGIGKALALQLANKDIFVIIADINETSGQDLVNNIKNNNQLARFEY 59 Query: 61 VDVADGPAV 69 +DV + +V Sbjct: 60 LDVTNAESV 68 >C3C310.1 C3C310_BACTU Short chain dehydrogenase Length = 271 Score = 134 bits (338), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 64/135 (47%), Positives = 81/135 (60%) Query: 45 TVAELEGLGGACRFVQVDVADGPAVGLTASLHYEAEAYGVKVSCLCPAFVDTPIFDSGEA 104 A GLG A VGLT SLHYEAE YGV VS LCP FVDTPIF+ A Sbjct: 137 NTASATGLGPAPLCTAYATTKHAIVGLTTSLHYEAEEYGVNVSVLCPTFVDTPIFEKSIA 196 Query: 105 VHMDXXXXXXXXXXXXXMTPEHFAGIAFKGIERNEELVNPMPLRRTMDVVFTLFPSLHRK 164 +HMD ++ + FA IA K I +N+ ++ PMP ++TMDV FTLFP LH+K Sbjct: 197 IHMDKGQIIKHLKTNKPISADKFAEIALKAIHKNKVVICPMPFKKTMDVFFTLFPYLHKK 256 Query: 165 VMRLVCRTTREARLS 179 +MR VCR R+A+++ Sbjct: 257 LMRFVCRIGRKAKMT 271 Score = 58.9 bits (141), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 28/69 (40%), Positives = 43/69 (62%), Gaps = 1/69 (1%) Query: 1 MDQIQRVGIVTGGASGIGKALCKELLKRRVYIVIADLNAEEGVKTVAELEGLGGACRFVQ 60 MD+ QR+ I+TGGASGIGKAL +L + ++++IAD+N G V ++ RF Sbjct: 1 MDE-QRIAIITGGASGIGKALALQLANKDIFVIIADINETSGQDLVNNIKNNNQLARFEY 59 Query: 61 VDVADGPAV 69 +DV + +V Sbjct: 60 LDVTNAESV 68 >Q737G7.1 Q737G7_BACC1 Short chain dehydrogenase family protein Length = 273 Score = 133 bits (334), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 63/135 (46%), Positives = 83/135 (61%) Query: 45 TVAELEGLGGACRFVQVDVADGPAVGLTASLHYEAEAYGVKVSCLCPAFVDTPIFDSGEA 104 A GLG A VGLT SLHYEAE YGV VS LCP FVDTPIF+ A Sbjct: 137 NTASATGLGPAPLCTAYATTKHAIVGLTTSLHYEAEEYGVNVSVLCPTFVDTPIFEKSIA 196 Query: 105 VHMDXXXXXXXXXXXXXMTPEHFAGIAFKGIERNEELVNPMPLRRTMDVVFTLFPSLHRK 164 ++M+ ++ + FA IA K I +N+ ++ PMPL++TMDV+FTLFP LH+K Sbjct: 197 INMNKSQIIKQLKTNKPISADKFAEIALKAIHKNKLVICPMPLKKTMDVLFTLFPYLHKK 256 Query: 165 VMRLVCRTTREARLS 179 +MR VCR R+A+++ Sbjct: 257 LMRFVCRIGRKAKMN 271 Score = 59.7 bits (143), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 30/69 (43%), Positives = 44/69 (63%), Gaps = 1/69 (1%) Query: 1 MDQIQRVGIVTGGASGIGKALCKELLKRRVYIVIADLNAEEGVKTVAELEGLGGACRFVQ 60 MD+ QRV IVTGGASGIGKAL +L + ++++IAD+N G + V ++ RF Sbjct: 1 MDE-QRVAIVTGGASGIGKALAIQLANKDIFVIIADINETSGQELVNNIKNNNQLARFEY 59 Query: 61 VDVADGPAV 69 +DV + +V Sbjct: 60 LDVTNAGSV 68 >C3H1J8.1 C3H1J8_BACTU Short chain dehydrogenase Length = 282 Score = 132 bits (332), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 63/135 (46%), Positives = 82/135 (60%) Query: 45 TVAELEGLGGACRFVQVDVADGPAVGLTASLHYEAEAYGVKVSCLCPAFVDTPIFDSGEA 104 A GLG A VGLT SLHYEAE YGV VS LCP FVDTPIF+ A Sbjct: 146 NTASATGLGPAPLCTAYATTKHAIVGLTTSLHYEAEEYGVNVSVLCPTFVDTPIFEKSIA 205 Query: 105 VHMDXXXXXXXXXXXXXMTPEHFAGIAFKGIERNEELVNPMPLRRTMDVVFTLFPSLHRK 164 ++M+ ++ + FA IA K I +N+ ++ PMPL++TMDV FTLFP LH+K Sbjct: 206 INMNKNQIIKQLKTNKPISADKFAEIALKAIHKNKLVICPMPLKKTMDVFFTLFPYLHKK 265 Query: 165 VMRLVCRTTREARLS 179 +MR VCR R+A+++ Sbjct: 266 LMRFVCRIGRKAKMT 280 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 956,853,134 Number of extensions: 33826918 Number of successful extensions: 128163 Number of sequences better than 1.0e-05: 77 Number of HSP's gapped: 129067 Number of HSP's successfully gapped: 95 Length of query: 179 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 52 Effective length of database: 1,864,227,298 Effective search space: 96939819496 Effective search space used: 96939819496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 126 (53.1 bits)