BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0046.p (344 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG00.1 B3KG00_9BACI Spore protease EC=3.4.24.78 538 e-151 B1DFE5.1 B1DFE5_9BACL Spore protease EC=3.4.24.78 464 e-129 C2V0R6.1 C2V0R6_BACCE Germination protease 372 e-101 C2X2T7.1 C2X2T7_BACCE Germination protease 372 e-101 C2VH34.1 C2VH34_BACCE Germination protease 372 e-101 >B3KG00.1 B3KG00_9BACI Spore protease EC=3.4.24.78 Length = 333 Score = 538 bits (1386), Expect = e-151, Method: Compositional matrix adjust. Identities = 263/336 (78%), Positives = 300/336 (89%), Gaps = 3/336 (0%) Query: 9 MELDLQLYSVRTDLALEAKEMAQGPQKTPIPGVNEEVEEADGIKVTRLAVANDAGSQAIG 68 M+LDLQ YSVRTDLA+E++E+A G PIPGV E+V + DGIK+TRL V NDA SQ IG Sbjct: 1 MDLDLQKYSVRTDLAVESRELAGGTGSAPIPGVMEDVVQEDGIKITRLDVENDAASQQIG 60 Query: 69 RAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLIVGLGNWNVTP 128 R G+YVTLEVP LR GDTGLQ +VSVVFAREFE FM RIGIG ASVLIVGLGNWNVTP Sbjct: 61 RVKGHYVTLEVPDLRKGDTGLQDQVSVVFAREFEAFMSRIGIGPKASVLIVGLGNWNVTP 120 Query: 129 DSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEVVQGIIDRTKP 188 DSLGPLVVEN ++TRQ++EL+PD+V+PGYR VSA+APGVLGLTGIESSE VQGI+++T+P Sbjct: 121 DSLGPLVVENVMVTRQYFELMPDRVAPGYRQVSAVAPGVLGLTGIESSETVQGIVEQTRP 180 Query: 189 DVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPCIAIGVPTVCY 248 ++IIAIDALASRSLER+NTTIQIADIGIHPGSG+GNKR+GLT+EILG+PCIAIGVPTVCY Sbjct: 181 ELIIAIDALASRSLERVNTTIQIADIGIHPGSGVGNKRKGLTQEILGVPCIAIGVPTVCY 240 Query: 249 ASTIVNNVLEMMSRHFGQAGAGATQTKEIMGMLDDISEHERLSLVKEVLEPLGHDLIVTP 308 ASTIVNN +E+M HFG+ GA +T +IMGML DI E ERL+LVKEVLEPLGHDLIVTP Sbjct: 241 ASTIVNNAIELMKSHFGE---GAPETGKIMGMLHDIPEGERLALVKEVLEPLGHDLIVTP 297 Query: 309 KEIDEFIEEIANIVASGLNAALHEAVDPGNVGAYTH 344 KEIDEFIE+IANIVA+GLNAALHEAVDPGNVGAYTH Sbjct: 298 KEIDEFIEDIANIVATGLNAALHEAVDPGNVGAYTH 333 >B1DFE5.1 B1DFE5_9BACL Spore protease EC=3.4.24.78 Length = 335 Score = 464 bits (1195), Expect = e-129, Method: Compositional matrix adjust. Identities = 234/338 (69%), Positives = 278/338 (82%), Gaps = 4/338 (1%) Query: 8 IMELDLQLYSVRTDLALEAKEMA-QGPQKTPIPGVNEEVEEADGIKVTRLAVANDAGSQA 66 + ELDLQ Y++RTDLALEAKE+A Q PIPG++ E E DGI +T L V N+ GS+A Sbjct: 1 MTELDLQQYNIRTDLALEAKEIAEQRGGGGPIPGIHSETTEDDGITITHLHVQNEQGSRA 60 Query: 67 IGRAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLIVGLGNWNV 126 IG+ +G YVT+EVP LR DT LQ +V+ FAR FE F++RIGIG+ A VLIVGLGNWNV Sbjct: 61 IGKVLGRYVTIEVPGLRKQDTELQDRVATHFARHFETFLERIGIGKTAKVLIVGLGNWNV 120 Query: 127 TPDSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEVVQGIIDRT 186 TPD+LGPLVVEN ++TR F+EL+PDQV+PGYR VSA+APGVLG+TGIESSE+VQGI ++T Sbjct: 121 TPDALGPLVVENVMVTRHFFELMPDQVAPGYRAVSAVAPGVLGITGIESSEIVQGITEKT 180 Query: 187 KPDVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPCIAIGVPTV 246 PDVIIAIDALAS+SL+R+NTTIQIADIGIHPGSGIGNKR+GLTKEILGIP IAIGVPTV Sbjct: 181 HPDVIIAIDALASKSLDRVNTTIQIADIGIHPGSGIGNKRKGLTKEILGIPVIAIGVPTV 240 Query: 247 CYASTIVNNVLEMMSRHFGQAGAGATQTKEIMGMLDDISEHERLSLVKEVLEPLGHDLIV 306 YASTIVN+ +E+M H A Q I G+++ + E ERL LVKEVL P+GHDL+V Sbjct: 241 VYASTIVNHTIELMRGHMAANNANNDQ---IFGLMNQMGEAERLQLVKEVLNPVGHDLLV 297 Query: 307 TPKEIDEFIEEIANIVASGLNAALHEAVDPGNVGAYTH 344 TPKEIDEFIE+IANI+ASGLNA+LHEAVD NV AYTH Sbjct: 298 TPKEIDEFIEDIANIIASGLNASLHEAVDSDNVAAYTH 335 >C2V0R6.1 C2V0R6_BACCE Germination protease Length = 374 Score = 372 bits (955), Expect = e-101, Method: Compositional matrix adjust. Identities = 186/364 (51%), Positives = 258/364 (70%), Gaps = 31/364 (8%) Query: 11 LDLQLYSVRTDLALEAKEMAQGPQKTP--IPGVNEEVEEADGIKVTRLAVANDAGSQAIG 68 LDL YSVRTDLA+EA +M Q Q+ + GV + E +GI +T++ + ++ S+++G Sbjct: 12 LDLSKYSVRTDLAVEAHQMLQERQEEQKGVQGVVVKEREEEGITITKVTI-DEVASESMG 70 Query: 69 RAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLIVGLGNWNVTP 128 + GNY+TLEV +R DT LQQKV +FA+EF F++ +G+ + AS LIVGLGNWNVTP Sbjct: 71 KKPGNYLTLEVQGIRQQDTELQQKVERIFAKEFSYFLEEVGVTKEASCLIVGLGNWNVTP 130 Query: 129 DSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEVVQGIIDRTKP 188 D+LGP+VVEN L+TR ++L P+ V G+R VSAI PGV+G+TGIE+S+V+ GII++TKP Sbjct: 131 DALGPIVVENVLVTRHLFQLQPESVEEGFRPVSAIRPGVMGITGIETSDVIYGIIEKTKP 190 Query: 189 DVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPCIAIGVPTVCY 248 D +IAIDALA+RS+ER+N+TIQI+D GIHPGSG+GNKR+ L+KE LGIP IAIGVPTV Sbjct: 191 DFVIAIDALAARSIERVNSTIQISDTGIHPGSGVGNKRKELSKETLGIPVIAIGVPTVVD 250 Query: 249 ASTIVNNVLEMMSRHFGQ--------------AGAGATQTKEI--------------MGM 280 A +I ++ ++ + +HFG+ AG + K++ +G Sbjct: 251 AVSITSDTIDFILKHFGREMKEGHKPSRSLLPAGFSFGEKKKLTEEDMPDEKSRNMFLGA 310 Query: 281 LDDISEHERLSLVKEVLEPLGHDLIVTPKEIDEFIEEIANIVASGLNAALHEAVDPGNVG 340 + + E E+ L+ EVL PLGH+L+VTPKE+D FIE++AN++ASGLNAALH +D N G Sbjct: 311 IGTLEEEEKRKLIYEVLSPLGHNLMVTPKEVDAFIEDMANVIASGLNAALHHQIDQDNTG 370 Query: 341 AYTH 344 AYTH Sbjct: 371 AYTH 374 >C2X2T7.1 C2X2T7_BACCE Germination protease Length = 374 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 185/364 (50%), Positives = 258/364 (70%), Gaps = 31/364 (8%) Query: 11 LDLQLYSVRTDLALEAKEMAQGPQKTP--IPGVNEEVEEADGIKVTRLAVANDAGSQAIG 68 LDL YSVRTDLA+EA +M Q Q+ + GV + E +GI +T++ + ++ S+++G Sbjct: 12 LDLSKYSVRTDLAVEAHQMLQERQEEQEGVQGVIVKEREEEGITITKVTI-DEVASESMG 70 Query: 69 RAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLIVGLGNWNVTP 128 + GNY+TLEV +R DT LQQKV +FA+EF F++ +G+ + AS LIVGLGNWNVTP Sbjct: 71 KKPGNYLTLEVQGIRQQDTELQQKVERIFAKEFSYFLEEVGVTKEASCLIVGLGNWNVTP 130 Query: 129 DSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEVVQGIIDRTKP 188 D+LGP+VVEN L+TR ++L P+ + G+R VSAI PGV+G+TGIE+S+V+ GII++TKP Sbjct: 131 DALGPIVVENVLVTRHLFQLQPESIEEGFRPVSAIRPGVMGITGIETSDVIYGIIEKTKP 190 Query: 189 DVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPCIAIGVPTVCY 248 D +IAIDALA+RS+ER+N+TIQI+D GIHPGSG+GNKR+ L+KE LGIP IAIGVPTV Sbjct: 191 DFVIAIDALAARSIERVNSTIQISDTGIHPGSGVGNKRKELSKETLGIPVIAIGVPTVVD 250 Query: 249 ASTIVNNVLEMMSRHFGQ--------------AGAGATQTKEI--------------MGM 280 A +I ++ ++ + +HFG+ AG + K++ +G Sbjct: 251 AVSITSDTIDFILKHFGREMKEGHKPSRSLLPAGFSFGEKKKLTEEDMPDEKSRNMFLGA 310 Query: 281 LDDISEHERLSLVKEVLEPLGHDLIVTPKEIDEFIEEIANIVASGLNAALHEAVDPGNVG 340 + + E E+ L+ EVL PLGH+L+VTPKE+D FIE++AN++ASGLNAALH +D N G Sbjct: 311 IGTLEEEEKRKLIYEVLSPLGHNLMVTPKEVDAFIEDMANVIASGLNAALHHQIDQDNTG 370 Query: 341 AYTH 344 AYTH Sbjct: 371 AYTH 374 >C2VH34.1 C2VH34_BACCE Germination protease Length = 374 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 185/364 (50%), Positives = 258/364 (70%), Gaps = 31/364 (8%) Query: 11 LDLQLYSVRTDLALEAKEMAQGPQKTP--IPGVNEEVEEADGIKVTRLAVANDAGSQAIG 68 LDL YSVRTDLA+EA +M Q Q+ + GV + E +GI +T++ + ++ S+++G Sbjct: 12 LDLSKYSVRTDLAVEAHQMLQERQEEQEGVQGVIVKEREEEGITITKVTI-DEVASESMG 70 Query: 69 RAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLIVGLGNWNVTP 128 + GNY+TLEV +R DT LQQKV +FA+EF F++ +G+ + AS LIVGLGNWNVTP Sbjct: 71 KKPGNYLTLEVQGIRQQDTELQQKVERIFAKEFSYFLEEVGVTKEASCLIVGLGNWNVTP 130 Query: 129 DSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEVVQGIIDRTKP 188 D+LGP+VVEN L+TR ++L P+ + G+R VSAI PGV+G+TGIE+S+V+ GII++TKP Sbjct: 131 DALGPIVVENVLVTRHLFQLQPESIEEGFRPVSAIRPGVMGITGIETSDVIYGIIEKTKP 190 Query: 189 DVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPCIAIGVPTVCY 248 D +IAIDALA+RS+ER+N+TIQI+D GIHPGSG+GNKR+ L+KE LGIP IAIGVPTV Sbjct: 191 DFVIAIDALAARSIERVNSTIQISDTGIHPGSGVGNKRKELSKETLGIPVIAIGVPTVVD 250 Query: 249 ASTIVNNVLEMMSRHFGQ--------------AGAGATQTKEI--------------MGM 280 A +I ++ ++ + +HFG+ AG + K++ +G Sbjct: 251 AVSITSDTIDFILKHFGREMKEGHKPSRSLLPAGFSFGEKKKLTEEDMPDEKSRNMFLGA 310 Query: 281 LDDISEHERLSLVKEVLEPLGHDLIVTPKEIDEFIEEIANIVASGLNAALHEAVDPGNVG 340 + + E E+ L+ EVL PLGH+L+VTPKE+D FIE++AN++ASGLNAALH +D N G Sbjct: 311 IGTLEEEEKRKLIYEVLSPLGHNLMVTPKEVDAFIEDMANVIASGLNAALHHQIDQDNTG 370 Query: 341 AYTH 344 AYTH Sbjct: 371 AYTH 374 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.136 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,974,172,377 Number of extensions: 82981017 Number of successful extensions: 239131 Number of sequences better than 1.0e-05: 197 Number of HSP's gapped: 238660 Number of HSP's successfully gapped: 197 Length of query: 344 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 208 Effective length of database: 1,779,438,163 Effective search space: 370123137904 Effective search space used: 370123137904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)