BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0038.p (359 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3J399.1 C3J399_9BACI Transcriptional regulator, LacI family 317 1e-84 B7YCT4.1 B7YCT4_9BACI Transcriptional regulator, LacI family 317 1e-84 A6LYR2.1 A6LYR2_CLOB8 Regulatory protein, LacI 315 3e-84 Q5KYK9.1 Q5KYK9_GEOKA Transcriptional regulator (LacI family) 313 2e-83 A7Z587.1 A7Z587_BACA2 KdgR 311 5e-83 >C3J399.1 C3J399_9BACI Transcriptional regulator, LacI family Length = 338 Score = 317 bits (812), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 156/335 (46%), Positives = 224/335 (66%), Gaps = 1/335 (0%) Query: 19 MKKLTIEDVAQKAGVSKSTVSQFLNKRFKYMSEATKNRIAEVIEELNYQPNGLARSLKQN 78 MKK+T+ DVA+ A VSKSTVSQ+LNKR++YMSE T+ RIAE IEEL YQPN +ARSLKQ Sbjct: 1 MKKVTMADVAKLANVSKSTVSQYLNKRYEYMSEETRKRIAEAIEELGYQPNVIARSLKQK 60 Query: 79 RTHMVGIIVANIDYSLSVQCIRAIENELQRHGIQVIICNSDENADKENTHVEALVARQVD 138 T +G+IV N+ + ++ Q + AIE E Q+ G QVI+CNS ++ DKE T++E L+A+QVD Sbjct: 61 TTATIGVIVFNMLHMMTTQVLHAIEEECQKRGFQVIVCNSGDDPDKEKTYIETLLAKQVD 120 Query: 139 GLIIFPAGDNPSPYSRLIEAEYPLVFMDRLVEGITTQSLLLDNEMAVKTAIKELTGKGHE 198 G+I+FP G N Y RL+E +PLVF+DR+V G+ S+LLDN A + A++ G+E Sbjct: 121 GMIVFPTGKNEEVYRRLVEERFPLVFVDRMVPGVGADSVLLDNAGAAEMAVQYFASYGYE 180 Query: 199 AIAILSLPLGEHAITPRKERMSGYKKAMEEAGLAMNDRYVRSVPREAIASALDELLILPQ 258 IAI++ PL + + PR ER G++ AME+ G + D+ V + A+ L + L P+ Sbjct: 181 RIAIVTPPLVPYNV-PRMERFEGFRAAMEKRGFEVGDQNVIAAEPAALPEVLKQRLAEPK 239 Query: 259 PPTALLAANDLVLGEILKYANRHSITIPGQLSVIGIDDAEFARIYNPAITTIRQPAYEMG 318 P AL A NDL L +L++ I +P +L++I IDD FA IY PA+TTI QP + MG Sbjct: 240 RPNALFAINDLTLMGVLRFVKETGIRMPDELAIINIDDVPFADIYTPALTTIAQPTFAMG 299 Query: 319 MQAAKIMLSLIEAQEAAVPITYRFPPALQQGQSVQ 353 +AA+ + ++A++ P +RF P L + S++ Sbjct: 300 KKAAERVFDQMKAKKTDGPRVFRFSPVLMERTSIR 334 >B7YCT4.1 B7YCT4_9BACI Transcriptional regulator, LacI family Length = 338 Score = 317 bits (812), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 156/335 (46%), Positives = 224/335 (66%), Gaps = 1/335 (0%) Query: 19 MKKLTIEDVAQKAGVSKSTVSQFLNKRFKYMSEATKNRIAEVIEELNYQPNGLARSLKQN 78 MKK+T+ DVA+ A VSKSTVSQ+LNKR++YMSE T+ RIAE IEEL YQPN +ARSLKQ Sbjct: 1 MKKVTMADVAKLANVSKSTVSQYLNKRYEYMSEETRKRIAEAIEELGYQPNVIARSLKQK 60 Query: 79 RTHMVGIIVANIDYSLSVQCIRAIENELQRHGIQVIICNSDENADKENTHVEALVARQVD 138 T +G+IV N+ + ++ Q + AIE E Q+ G QVI+CNS ++ DKE T++E L+A+QVD Sbjct: 61 TTATIGVIVFNMLHMMTTQVLHAIEEECQKRGFQVIVCNSGDDPDKEKTYIETLLAKQVD 120 Query: 139 GLIIFPAGDNPSPYSRLIEAEYPLVFMDRLVEGITTQSLLLDNEMAVKTAIKELTGKGHE 198 G+I+FP G N Y RL+E +PLVF+DR+V G+ S+LLDN A + A++ G+E Sbjct: 121 GMIVFPTGKNEEVYRRLVEERFPLVFVDRMVPGVGADSVLLDNAGAAEMAVQYFASYGYE 180 Query: 199 AIAILSLPLGEHAITPRKERMSGYKKAMEEAGLAMNDRYVRSVPREAIASALDELLILPQ 258 IAI++ PL + + PR ER G++ AME+ G + D+ V + A+ L + L P+ Sbjct: 181 RIAIVTPPLVPYNV-PRMERFEGFRAAMEKRGFEVGDQNVIAAEPAALPEVLKQRLAEPK 239 Query: 259 PPTALLAANDLVLGEILKYANRHSITIPGQLSVIGIDDAEFARIYNPAITTIRQPAYEMG 318 P AL A NDL L +L++ I +P +L++I IDD FA IY PA+TTI QP + MG Sbjct: 240 RPNALFAINDLTLMGVLRFVKETGIRMPDELAIINIDDVPFADIYTPALTTIAQPTFAMG 299 Query: 319 MQAAKIMLSLIEAQEAAVPITYRFPPALQQGQSVQ 353 +AA+ + ++A++ P +RF P L + S++ Sbjct: 300 KKAAERVFDQMKAKKTDGPRVFRFSPVLMERTSIR 334 >A6LYR2.1 A6LYR2_CLOB8 Regulatory protein, LacI Length = 345 Score = 315 bits (808), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 150/341 (43%), Positives = 229/341 (67%), Gaps = 4/341 (1%) Query: 21 KLTIEDVAQKAGVSKSTVSQFLNKRFKYMSEATKNRIAEVIEELNYQPNGLARSLKQNRT 80 K+TI++VA++A VSKSTVSQFLN+RF+YMS+ TK +I IEEL+YQPN LARSLKQ RT Sbjct: 2 KVTIKEVAERANVSKSTVSQFLNERFEYMSDDTKKKIEMAIEELDYQPNQLARSLKQKRT 61 Query: 81 HMVGIIVANIDYSLSVQCIRAIENELQRHGIQVIICNSDENADKENTHVEALVARQVDGL 140 +++GI+ + I +++ +RA+E+ Q+ GIQV+ICN+D+N +KE V+++++RQVDGL Sbjct: 62 NVIGIVASTIRSRFTIEIVRAVEDVCQKKGIQVLICNTDDNPEKEKYCVDSMISRQVDGL 121 Query: 141 IIFPAGDNPSPYSRLIEAEYPLVFMDRLVEGITTQSLLLDNEMAVKTAIKELTGKGHEAI 200 I+FP N Y ++ YPLV +DR ++ I +++LDN +A K A+ L GH+ I Sbjct: 122 IVFPTRHNIELYQNIVSRNYPLVLIDRRIKEINASTVVLDNLLASKKAVDLLVENGHKKI 181 Query: 201 AILSLPLGEHAITPRKERMSGYKKAMEEAGLAMNDRYVRSVPREAIASALDELLILPQPP 260 ILS P+ ++I+ R +R+ GY+ A+ G+++N +++S P + I+ + LL P Sbjct: 182 GILSFPVQNNSISNRVDRIKGYRNALRSHGISVNKNFIKSGPLDKISGMIQSLLNGKDKP 241 Query: 261 TALLAANDLVLGEILKYANRHSITIPGQLSVIGIDDAEFARIYNPAITTIRQPAYEMGMQ 320 TA++A+ND++L ++L Y + I+IP +LSVIGIDD FA Y P+ITTI QPAY+MG + Sbjct: 242 TAVIASNDMILEKLLVYVKENGISIPDELSVIGIDDVSFASFYQPSITTIAQPAYDMGKE 301 Query: 321 AAKIMLSLIEAQEAAVP----ITYRFPPALQQGQSVQAPPG 357 AK++ IE + +F P L + +SV G Sbjct: 302 CAKLLFDQIEYNNGNLTSNNICNIKFEPILLERESVSDLLG 342 >Q5KYK9.1 Q5KYK9_GEOKA Transcriptional regulator (LacI family) Length = 338 Score = 313 bits (801), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 154/335 (45%), Positives = 222/335 (66%), Gaps = 1/335 (0%) Query: 19 MKKLTIEDVAQKAGVSKSTVSQFLNKRFKYMSEATKNRIAEVIEELNYQPNGLARSLKQN 78 MKK+T+ DVA+ A VSKSTVSQ+LNKR++YMSE T+ RIA+ IEEL YQPN +ARSLKQ Sbjct: 1 MKKVTMADVAKLANVSKSTVSQYLNKRYEYMSEETRKRIAQAIEELGYQPNVIARSLKQK 60 Query: 79 RTHMVGIIVANIDYSLSVQCIRAIENELQRHGIQVIICNSDENADKENTHVEALVARQVD 138 T +G+IV N+ + L+ Q + AIE E Q+ G QVI+CNS ++ +KE +V+ L+A+QVD Sbjct: 61 TTATIGVIVFNMLHMLTTQVLHAIEEECQKRGFQVIVCNSGDDPEKEKKYVDMLLAKQVD 120 Query: 139 GLIIFPAGDNPSPYSRLIEAEYPLVFMDRLVEGITTQSLLLDNEMAVKTAIKELTGKGHE 198 GLI+FP G N Y RL+E +PLVF+DR+V G+ S+LLDN A + A+ T G+E Sbjct: 121 GLIVFPTGKNEEVYRRLVEERFPLVFVDRMVPGVEADSVLLDNAGAAEMAVHYFTSYGYE 180 Query: 199 AIAILSLPLGEHAITPRKERMSGYKKAMEEAGLAMNDRYVRSVPREAIASALDELLILPQ 258 IAI++ PL + + PR ER G++ AM + GL + DR + +A+ L + P+ Sbjct: 181 RIAIVTPPLVPYNV-PRMERFEGFRAAMRKRGLDVEDRNIIVADPDALPEVLKQRFAEPK 239 Query: 259 PPTALLAANDLVLGEILKYANRHSITIPGQLSVIGIDDAEFARIYNPAITTIRQPAYEMG 318 P AL A NDL L +L++ I +P +L++I IDD FA IY PA+TTI QP + MG Sbjct: 240 RPNALFAINDLTLMGVLRFVKETGIRMPAELAIINIDDVPFADIYTPALTTIAQPTFAMG 299 Query: 319 MQAAKIMLSLIEAQEAAVPITYRFPPALQQGQSVQ 353 +AA+ + ++A++ P +RF P L + +++ Sbjct: 300 KKAAERVFDQMKAKKTDGPRVFRFSPVLMERTAIR 334 >A7Z587.1 A7Z587_BACA2 KdgR Length = 333 Score = 311 bits (798), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 153/333 (45%), Positives = 218/333 (65%), Gaps = 1/333 (0%) Query: 19 MKKLTIEDVAQKAGVSKSTVSQFLNKRFKYMSEATKNRIAEVIEELNYQPNGLARSLKQN 78 MK +TI DVA+ A VSKSTVSQFLN+R+ YMSE TK +I I+ELNYQPN +ARSLKQ Sbjct: 1 MKTITISDVAKYANVSKSTVSQFLNQRYDYMSEKTKQKIEAAIQELNYQPNFVARSLKQK 60 Query: 79 RTHMVGIIVANIDYSLSVQCIRAIENELQRHGIQVIICNSDENADKENTHVEALVARQVD 138 T VG++VANI ++ S Q IRA+E+ G +I+CN+D+ +KE ++E L A+QVD Sbjct: 61 STFTVGVVVANILHTFSTQVIRAVEDYFHEQGFHIIVCNADDEPEKEKKYIEMLRAKQVD 120 Query: 139 GLIIFPAGDNPSPYSRLIEAEYPLVFMDRLVEGITTQSLLLDNEMAVKTAIKELTGKGHE 198 G+IIFP G+N S Y ++ +P+VFMDR +E + +++LDN A A+ G + Sbjct: 121 GIIIFPTGENLSLYEKMKRDTFPVVFMDRTIEELGIPTVMLDNHHAAGLAVDRFIESGIK 180 Query: 199 AIAILSLPLGEHAITPRKERMSGYKKAMEEAGLAMNDRYVRSVPREAIASALDELLILPQ 258 IAI++ + I+PR ER+ GYKKA++ G+ + D Y+++ I++ L EL L + Sbjct: 181 RIAIITTSIIRE-ISPRVERIEGYKKALDRHGMMVRDEYIKTADAADISNVLSELFSLEE 239 Query: 259 PPTALLAANDLVLGEILKYANRHSITIPGQLSVIGIDDAEFARIYNPAITTIRQPAYEMG 318 PP A+LAAND+VL E+LKY H +TIP + +VIGID+ FA + P ITTI QPA EM Sbjct: 240 PPKAILAANDIVLVEVLKYMKEHDMTIPDKAAVIGIDEVPFAGFFTPPITTIVQPAAEMA 299 Query: 319 MQAAKIMLSLIEAQEAAVPITYRFPPALQQGQS 351 +A ++L I+ ++ +R+ P L QS Sbjct: 300 RKAGSLLLRQIQEKDGGEKRIHRYKPVLLARQS 332 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,018,017,543 Number of extensions: 77457800 Number of successful extensions: 280028 Number of sequences better than 1.0e-05: 8942 Number of HSP's gapped: 261241 Number of HSP's successfully gapped: 8945 Length of query: 359 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 222 Effective length of database: 1,770,017,148 Effective search space: 392943806856 Effective search space used: 392943806856 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)