BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0037.p (340 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I8W2.1 B8I8W2_CLOCE Transcriptional regulator, LacI family 144 1e-32 Q5WL99.1 Q5WL99_BACSK Transcriptional regulator 144 2e-32 B3KD03.1 B3KD03_9BACI Regulatory protein LacI 143 2e-32 B0MNL2.1 B0MNL2_9FIRM Putative uncharacterized protein 140 2e-31 B0K7Z0.1 B0K7Z0_THEP3 Transcriptional regulator, LacI family 138 8e-31 >B8I8W2.1 B8I8W2_CLOCE Transcriptional regulator, LacI family Length = 346 Score = 144 bits (363), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 107/336 (31%), Positives = 175/336 (52%), Gaps = 14/336 (4%) Query: 14 TKKVTITDIAKAMGLSPVSVSRALANQAGISDELKAKIVNKAAEMGY---IKPRKHTPAR 70 +KKVT+ DIA+ +G+S +VS AL GIS+ + I A EMGY + RK+T AR Sbjct: 2 SKKVTMDDIAEKLGISKNTVSLALRGMPGISESTRKVIEQTAREMGYTYKVSARKNTMAR 61 Query: 71 ILVLHQKPYQHDNSNF-SYMVQGIEKALQEADTDYSIEFLDKDNQSRLILPYRLSKGFKF 129 L L D+ F SY+ GIE ++ + + I + D++ Q P + G Sbjct: 62 NLCLIIAKSTRDSIGFFSYVQLGIEDEAKKNNLNTIIHYYDENVQG-FETPNCVKDGM-V 119 Query: 130 DGVIFIGRFNPEYVSFIQQEIPSLIFYVGYSPAYDYDSVWFSFLNAGYKQCKYLIDRGHS 189 G+I +GR + E ++ I ++ Y D + + GY +YLID GH+ Sbjct: 120 SGIITLGRISRETINCIVGYNLPVVMVDNYFDNLSMDYILTDNHSGGYAATEYLIDCGHT 179 Query: 190 RIGFLGDLSAYRN-KEKKTGITSALEEHGLTVDE--ALFFGRNEAFPAR-LAGLI----T 241 +IGFLGD+SA + ++ G AL + G+ ++E ++ + E P + GL+ T Sbjct: 180 KIGFLGDISASISFYDRYQGFLKALRDRGIEINEGYSITDKKLEELPQEDITGLVNEIRT 239 Query: 242 DGLLPTAFICEHDFAAVELIRLLQGHGLKVPGDVSILSSGNTEVSAYSLPALTTMDLNIE 301 LPTAF C +D A+ +I++L+ G+ VP +SI+ + E +A P LTTM + E Sbjct: 240 KAGLPTAFFCCNDAEAIVIIKVLKNIGVLVPNKISIIGFDDIENAANVTPELTTMRVQKE 299 Query: 302 YSCRTVVSTLLKRIAEPGKPAENIAVLSTLVERESV 337 + V L++++ + K +E I + + L++R SV Sbjct: 300 IMGKGAVCKLMEKLEQEIKSSEKILLSACLIKRNSV 335 >Q5WL99.1 Q5WL99_BACSK Transcriptional regulator Length = 334 Score = 144 bits (362), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 108/342 (31%), Positives = 178/342 (52%), Gaps = 28/342 (8%) Query: 17 VTITDIAKAMGLSPVSVSRALANQAGISDELKAKIVNKAAEMGYIKPRKHTPAR------ 70 VT+ DIAK G+S V+VS+AL ++ G+SDE+K KI AA+MGY R H A+ Sbjct: 2 VTMGDIAKKAGVSTVTVSKALNDKEGVSDEMKRKIKQMAADMGY---RFHAAAKSMRSGR 58 Query: 71 ---ILVLHQKPYQHDNSNFSY-MVQGIEKALQEADTDYSIEFLDKDNQSRLILPYRLSKG 126 I++L + + D F + I L+ D ++ L D++ RL+LP K Sbjct: 59 TDNIVILIPERFAGDEQAFYLKFYRQIISVLESYDYYGILQILSSDDEERLMLP----KA 114 Query: 127 F---KFDGVIFIGRFNPEYVSFIQQEIPSLIFYVGYSPAYDYDSVWFSFLNAGYKQCKYL 183 F + DGVI +G+ EY+ + +E L+F Y+ + D V A Y+ YL Sbjct: 115 FLENRADGVIVLGQPKKEYIEHVTKENVPLVFLDFYTDQLNADVVVTDNFYAVYELTNYL 174 Query: 184 IDRGHSRIGFLGDLSAYRN-KEKKTGITSALEEHGLTVDEA-LFFGRNEAFPARLAGLIT 241 I +GH IGF+G++ + +++ G+ +L EH L +E L R++A R L+ Sbjct: 175 ISQGHREIGFVGNVHTTSSIQDRFLGMYKSLLEHRLPFNERYLVSDRDDA--GRFIDLVL 232 Query: 242 DGLLPTAFICEHDFAAVELIRLLQGHGLKVPGDVSILSSGNTEVSAYSLPALTTMDLNIE 301 LPTAF+C D LI+ L G++VP D S++ N + S P LTT+++++E Sbjct: 233 PEKLPTAFVCNCDHVGYLLIKKLSDSGIRVPEDCSVVGFDNDIYATLSTPPLTTVEVDLE 292 Query: 302 YSCRTVVSTLLKRIAEPGKPAE---NIAVLSTLVERESVRTL 340 + +++++ E K ++ I + ++V RESV+ + Sbjct: 293 EMAKVATKRMIEKL-EGDKGSDGYGRILIKGSIVFRESVKNI 333 >B3KD03.1 B3KD03_9BACI Regulatory protein LacI Length = 334 Score = 143 bits (360), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 99/335 (29%), Positives = 170/335 (50%), Gaps = 16/335 (4%) Query: 14 TKKVTITDIAKAMGLSPVSVSRALANQAGISDELKAKIVNKAAEMGYIKPRKHTPARIL- 72 K +T+ DIA+ +G+S V+VS+AL ++ G+SDELKA+I A+EMGY R +T AR + Sbjct: 2 NKNITMKDIAEQVGVSTVTVSKALNDKEGVSDELKARIKAIASEMGY---RVNTVARSMR 58 Query: 73 ---------VLHQKPYQHDNSNFSYMVQGIEKALQEADTDYSIEFLDKDNQSRLILPYRL 123 ++ ++ D S + + Q I K L++ + L ++ L LP R+ Sbjct: 59 EGLTYNIGVIIPERFTGPDQSFYLRVYQEISKGLEDYGYYGILHILSSSDEQTLQLP-RI 117 Query: 124 SKGFKFDGVIFIGRFNPEYVSFIQQEIPSLIFYVGYSPAYDYDSVWFSFLNAGYKQCKYL 183 K DG I +G+ + Y+ + P ++F Y D D+V Y+ YL Sbjct: 118 YYDRKVDGFILLGQISKPYIEIMHAIEPPILFLDFYDDHSDIDAVVSDNFYGAYELTNYL 177 Query: 184 IDRGHSRIGFLGDLSAYRN-KEKKTGITSALEEHGLTVDEALFFGRNEAFPARLAGLITD 242 I +GH I ++G L A + +++ G +L EH + + + + L I D Sbjct: 178 IRQGHRNIAYVGSLHATSSIQDRFLGYYKSLLEHHIALRQDWVINDRNEYGNYLDIEIPD 237 Query: 243 GLLPTAFICEHDFAAVELIRLLQGHGLKVPGDVSILSSGNTEVSAYSLPALTTMDLNIEY 302 L PTAF+C D A +++ LQG G ++P D S++ N + ++P LTT+++N++ Sbjct: 238 EL-PTAFVCNCDQVAYLVVQKLQGMGYRIPDDCSVVGFDNDIYATLTVPHLTTVEVNMKQ 296 Query: 303 SCRTVVSTLLKRIAEPGKPAENIAVLSTLVERESV 337 +T + ++L +I+ P I V ++ R SV Sbjct: 297 LAQTSIQSILSKISTPDTIFGRIVVKGKIIYRSSV 331 >B0MNL2.1 B0MNL2_9FIRM Putative uncharacterized protein Length = 337 Score = 140 bits (353), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 109/351 (31%), Positives = 176/351 (50%), Gaps = 37/351 (10%) Query: 12 LKTKKVTITDIAKAMGLSPVSVSRALANQAGISDELKAKIVNKAAEMGY------IKPRK 65 +K K VT++DIAKAM +S V+VS+AL ++ G+S+EL+ KI KA EMGY ++ Sbjct: 1 MKKKSVTMSDIAKAMDISTVTVSKALGDKDGVSEELREKIKQKANEMGYRFSYTSKSSKE 60 Query: 66 HTPARILVLHQKPYQHDNSNFSY-MVQGIEKALQEADTDYSIEFLDKDNQSRLILPYRLS 124 I ++ K + +D S F + M + I + LQ+ + ++ + + +Q+ +P +S Sbjct: 61 GFTFNISIVVAKHFINDASAFYWVMYRHIVELLQKQNYYGILDVISESDQANRSMPNSVS 120 Query: 125 KGFKFDGVIFIGRFNPEYVS-FIQQEIPSLIFYVGYSPAYDYDSVWFSFLNAGYKQCKYL 183 K DGVI +G+F+ EYVS + +IP ++F YS D++ Y YL Sbjct: 121 DK-KVDGVIVLGQFDDEYVSELLDLDIP-VVFLDFYSSDNRTDTILSDSFFGSYMLTNYL 178 Query: 184 IDRGHSRIGFLGDLSAYRNKEKKTGITSALEEHGLTVDEALFFGRNEAFPARLAGLITD- 242 I+ GH IGFLG +++ TS++++ L +AL + P R +I D Sbjct: 179 INAGHRNIGFLGTITS----------TSSIQDRYLGYYKALL---EKGIPLRSEWVIDDR 225 Query: 243 -------------GLLPTAFICEHDFAAVELIRLLQGHGLKVPGDVSILSSGNTEVSAYS 289 PTAF+C D A L+ L+ G VP D+SI+ N S S Sbjct: 226 GPEGTSYPHFDLPAQKPTAFVCNCDEEAYCLLNQLRSEGYVVPDDISIVGYDNHIYSTIS 285 Query: 290 LPALTTMDLNIEYSCRTVVSTLLKRIAEPGKPAENIAVLSTLVERESVRTL 340 P +TT+D++ + V T++K+I + V L+ R+SV+ L Sbjct: 286 NPRITTIDVDSKRMSYEAVETIIKKIRDKNYSRGRTLVTGKLIIRDSVKIL 336 >B0K7Z0.1 B0K7Z0_THEP3 Transcriptional regulator, LacI family Length = 348 Score = 138 bits (347), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 99/351 (28%), Positives = 180/351 (51%), Gaps = 29/351 (8%) Query: 12 LKTKKVTITDIAKAMGLSPVSVSRALANQAGISDELKAKIVNKAAEMGYIK------PRK 65 +K KKVT+ DIA+ + LS +VS AL ++ G+S+E + ++ A EMGY + Sbjct: 4 IKRKKVTMKDIAERLNLSINAVSLALNDKVGVSEETRKIVLKTADEMGYFDENPSFIAKN 63 Query: 66 HTPARILVLHQKPYQHDNSNFSYMVQGIEKALQEADTDYSIEFLDKDNQSRLILPYRLSK 125 H L++ ++ ++ D ++ ++ GIE ++ + D + F+ NQ +P + Sbjct: 64 HFRNICLLIEERNFR-DTHFYTKVILGIENEAKKNNYDILVNFM---NQDDFQVPSSVET 119 Query: 126 GFKFDGVIFIGRFNPEYVS-FIQQEIPS-LIFYVGYSPAYDYDSVWFSFLNAGYKQCKYL 183 K G++ +G E++ ++ +IP+ L+ + ++ + D +V + Y +YL Sbjct: 120 R-KVSGILVVGTIKDEHLEKLLKYDIPTVLVDHASFTISTD--AVLTQNMPGTYMATQYL 176 Query: 184 IDRGHSRIGFLGDLS-AYRNKEKKTGITSALEEHGLTVDEAL-------FFGRNEAFP-- 233 I +GH++IGF G++ + KE+ G A+ GL VD G+ E + Sbjct: 177 IRKGHTQIGFFGEIDFSLSFKERWLGFNEAMRNAGLNVDPIFNVNPGYCVIGQVEQYVLS 236 Query: 234 ---ARLAGLITD-GLLPTAFICEHDFAAVELIRLLQGHGLKVPGDVSILSSGNTEVSAYS 289 +A +I+ LPTA++C +D AA+ L L G+KVP D+S++ + ++ Sbjct: 237 KNYKEVANIISKLDKLPTAWVCSNDSAAITLYNALSILGIKVPDDISVVGFDDIDICNIV 296 Query: 290 LPALTTMDLNIEYSCRTVVSTLLKRIAEPGKPAENIAVLSTLVERESVRTL 340 P LTT+ +N E V LL R+ P +P ++I + L+ERESV+ + Sbjct: 297 TPHLTTIRINKELMGEKAVKRLLWRMENPKEPCDHIRMEVKLIERESVKEI 347 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,949,662,814 Number of extensions: 81012868 Number of successful extensions: 234882 Number of sequences better than 1.0e-05: 4389 Number of HSP's gapped: 231023 Number of HSP's successfully gapped: 4389 Length of query: 340 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 204 Effective length of database: 1,779,438,163 Effective search space: 363005385252 Effective search space used: 363005385252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)