BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0030.p (341 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q4EN70.1 Q4EN70_LISMO Glyceraldehyde-3-phosphate dehydrogenase, ... 611 e-173 Q8Y4I1.1 Q8Y4I1_LISMO Gap protein 608 e-172 A0ALE4.1 A0ALE4_LISW6 Gap protein EC=1.2.1.12 608 e-172 Q928H9.1 Q928H9_LISIN Gap protein 605 e-171 Q71WW7.1 Q71WW7_LISMF Glyceraldehyde-3-phosphate dehydrogenase, ... 605 e-171 >Q4EN70.1 Q4EN70_LISMO Glyceraldehyde-3-phosphate dehydrogenase, type I EC=1.2.1.- Length = 336 Score = 611 bits (1575), Expect = e-173, Method: Compositional matrix adjust. Identities = 287/336 (85%), Positives = 319/336 (94%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 M++KVGINGFGRIGRLAFRRIQNVEG+EVVAINDLT+AKMLAHLLKYDTTQG F+G++EV Sbjct: 1 MTVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEV 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLKGGAKKVVIS 125 HDG+FKVNGKEVKVL NRNPEELPWG+LGVDI+LECTGF T ++KAELH+K GAKKVVIS Sbjct: 61 HDGFFKVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVIS 120 Query: 126 APATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHAY 185 APATGD+KTIVYNVNHETLDGTETVISGASCTTNCLAPMAK L DKFG++ GLMTT+HAY Sbjct: 121 APATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKVLEDKFGVVEGLMTTIHAY 180 Query: 186 TGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPTG 245 TG+QNTLDAP+PKGDFR ARAAAENI+P +TGAAKAIG VLP LKGKLDGA+QRVPVPTG Sbjct: 181 TGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTG 240 Query: 246 SVTELVSVLNTKVTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKVL 305 S+TELV+VL+ KVTVD+VNAAM+ ASDSET+GYT D++VSSDIKG+TFGSLFD TQTKVL Sbjct: 241 SLTELVTVLDKKVTVDEVNAAMETASDSETFGYTSDQVVSSDIKGMTFGSLFDETQTKVL 300 Query: 306 TVQDQQLVKTVAWYDNEMSYTSQLVRTLEYFAKIAK 341 TV DQQLVKTVAWYDNEMSYT+QLVRTLEYFAKIAK Sbjct: 301 TVGDQQLVKTVAWYDNEMSYTAQLVRTLEYFAKIAK 336 >Q8Y4I1.1 Q8Y4I1_LISMO Gap protein Length = 336 Score = 608 bits (1568), Expect = e-172, Method: Compositional matrix adjust. Identities = 286/336 (85%), Positives = 318/336 (94%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 M++KVGINGFGRIGRLAFRRIQNVEG+EVVAINDLT+AKMLAHLLKYDTTQG F+G++EV Sbjct: 1 MTVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEV 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLKGGAKKVVIS 125 HDG+FKVNGKEVKVL NRNPEELPWG+LGVDI+LECTGF T ++KAELH+K GAKKVVIS Sbjct: 61 HDGFFKVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVIS 120 Query: 126 APATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHAY 185 APATGD+KTIVYNVNHETLDGTETVISGASCTTNCLAPMAK L DKFG++ GLMTT+HAY Sbjct: 121 APATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKVLEDKFGVVEGLMTTIHAY 180 Query: 186 TGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPTG 245 TG+QNTLDAP+PKGDFR ARAAAENI+P +TGAAKAIG VLP LKGKLDGA+QRVPVPTG Sbjct: 181 TGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTG 240 Query: 246 SVTELVSVLNTKVTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKVL 305 S+TELV+VL+ KVTVD+VNAAM+ ASD ET+GYT D++VSSDIKG+TFGSLFD TQTKVL Sbjct: 241 SLTELVTVLDKKVTVDEVNAAMEAASDPETFGYTSDQVVSSDIKGMTFGSLFDETQTKVL 300 Query: 306 TVQDQQLVKTVAWYDNEMSYTSQLVRTLEYFAKIAK 341 TV DQQLVKTVAWYDNEMSYT+QLVRTLEYFAKIAK Sbjct: 301 TVGDQQLVKTVAWYDNEMSYTAQLVRTLEYFAKIAK 336 >A0ALE4.1 A0ALE4_LISW6 Gap protein EC=1.2.1.12 Length = 336 Score = 608 bits (1568), Expect = e-172, Method: Compositional matrix adjust. Identities = 286/336 (85%), Positives = 318/336 (94%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 M++KVGINGFGRIGRLAFRRIQNVEG+EVVAINDLT+AKMLAHLLKYDTTQG F+G++EV Sbjct: 1 MTVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEV 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLKGGAKKVVIS 125 HDG+FKVNGKEVKVL NRNPEELPWG+LGVDI+LECTGF T ++KAELH+K GAKKVVIS Sbjct: 61 HDGFFKVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVIS 120 Query: 126 APATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHAY 185 APATGD+KTIVYNVNHETLDGTETVISGASCTTNCLAPMAK L DKFG++ GLMTT+HAY Sbjct: 121 APATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKVLEDKFGVVEGLMTTIHAY 180 Query: 186 TGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPTG 245 TG+QNTLDAP+PKGDFR ARAAAENI+P +TGAAKAIG VLP LKGKLDGA+QRVPVPTG Sbjct: 181 TGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTG 240 Query: 246 SVTELVSVLNTKVTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKVL 305 S+TELV+VL+ KVTVD+VNAAM+ ASD ET+GYT D++VSSDIKG+TFGSLFD TQTKVL Sbjct: 241 SLTELVTVLDKKVTVDEVNAAMEAASDPETFGYTSDQVVSSDIKGMTFGSLFDETQTKVL 300 Query: 306 TVQDQQLVKTVAWYDNEMSYTSQLVRTLEYFAKIAK 341 TV DQQLVKTVAWYDNEMSYT+QLVRTLEYFAKIAK Sbjct: 301 TVGDQQLVKTVAWYDNEMSYTAQLVRTLEYFAKIAK 336 >Q928H9.1 Q928H9_LISIN Gap protein Length = 336 Score = 605 bits (1561), Expect = e-171, Method: Compositional matrix adjust. Identities = 285/336 (84%), Positives = 317/336 (94%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 M++KVGINGFGRIGRLAFRRIQNVEG+EVVAINDLT+AKMLAHLLKYDTTQG F+G++EV Sbjct: 1 MTVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEV 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLKGGAKKVVIS 125 HDG+F VNGKEVKVL NRNPEELPWG+LGVDI+LECTGF T ++KAELH+K GAKKVVIS Sbjct: 61 HDGFFNVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVIS 120 Query: 126 APATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHAY 185 APATGD+KTIVYNVNHETLDGTETVISGASCTTNCLAPMAK L DKFG++ GLMTT+HAY Sbjct: 121 APATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKVLEDKFGVVEGLMTTIHAY 180 Query: 186 TGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPTG 245 TG+QNTLDAP+PKGDFR ARAAAENI+P +TGAAKAIG VLP LKGKLDGA+QRVPVPTG Sbjct: 181 TGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTG 240 Query: 246 SVTELVSVLNTKVTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKVL 305 S+TELV+VL+ KVTVD+VNAAM+ ASD ET+GYT D++VSSDIKG+TFGSLFD TQTKVL Sbjct: 241 SLTELVTVLDKKVTVDEVNAAMEAASDPETFGYTSDQVVSSDIKGMTFGSLFDETQTKVL 300 Query: 306 TVQDQQLVKTVAWYDNEMSYTSQLVRTLEYFAKIAK 341 TV DQQLVKTVAWYDNEMSYT+QLVRTLEYFAKIAK Sbjct: 301 TVGDQQLVKTVAWYDNEMSYTAQLVRTLEYFAKIAK 336 >Q71WW7.1 Q71WW7_LISMF Glyceraldehyde-3-phosphate dehydrogenase, type I EC=1.2.1.- Length = 336 Score = 605 bits (1561), Expect = e-171, Method: Compositional matrix adjust. Identities = 285/336 (84%), Positives = 317/336 (94%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 M++KVGINGFGRIGRLAFRRIQNVEG+EVVAINDLT+AKMLAHLLKYDTTQG F+G++EV Sbjct: 1 MTVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEV 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLKGGAKKVVIS 125 HDG+F VNGKEVKVL NRNPEELPWG+LGVDI+LECTGF T ++KAELH+K GAKKVVIS Sbjct: 61 HDGFFNVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVIS 120 Query: 126 APATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHAY 185 APATGD+KTIVYNVNHETLDGTETVISGASCTTNCLAPMAK L DKFG++ GLMTT+HAY Sbjct: 121 APATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKVLEDKFGVVEGLMTTIHAY 180 Query: 186 TGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPTG 245 TG+QNTLDAP+PKGDFR ARAAAENI+P +TGAAKAIG VLP LKGKLDGA+QRVPVPTG Sbjct: 181 TGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTG 240 Query: 246 SVTELVSVLNTKVTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKVL 305 S+TELV+VL+ KVTVD+VNAAM+ ASD ET+GYT D++VSSDIKG+TFGSLFD TQTKVL Sbjct: 241 SLTELVTVLDKKVTVDEVNAAMEAASDPETFGYTSDQVVSSDIKGMTFGSLFDETQTKVL 300 Query: 306 TVQDQQLVKTVAWYDNEMSYTSQLVRTLEYFAKIAK 341 TV DQQLVKTVAWYDNEMSYT+QLVRTLEYFAKIAK Sbjct: 301 TVGDQQLVKTVAWYDNEMSYTAQLVRTLEYFAKIAK 336 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,955,561,316 Number of extensions: 81638950 Number of successful extensions: 200426 Number of sequences better than 1.0e-05: 7057 Number of HSP's gapped: 178008 Number of HSP's successfully gapped: 7059 Length of query: 341 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 205 Effective length of database: 1,779,438,163 Effective search space: 364784823415 Effective search space used: 364784823415 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)