BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0024.p (282 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFZ2.1 B3KFZ2_9BACI Pyrroline-5-carboxylate reductase EC=1.5.1.2 324 6e-87 B1DFF4.1 B1DFF4_9BACL Pyrroline-5-carboxylate reductase EC=1.5.1.2 287 6e-76 C0ZB14.1 C0ZB14_BREBN Putative uncharacterized protein 222 3e-56 C5D4V6.1 C5D4V6_9BACI Pyrroline-5-carboxylate reductase EC=1.5.1.2 216 2e-54 A7Z6X4.1 A7Z6X4_BACA2 ComER 213 2e-53 >B3KFZ2.1 B3KFZ2_9BACI Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 280 Score = 324 bits (830), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 159/272 (58%), Positives = 203/272 (74%), Gaps = 1/272 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIGS-NQETA 59 MKVGFIGTGSMGSLLIDAF+ SGAL P + SNRSP + LQL +RHPG+++ S N ETA Sbjct: 1 MKVGFIGTGSMGSLLIDAFIQSGALLPEQIRVSNRSPEKPLQLAKRHPGLTVCSCNTETA 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 SD++F+C+KPLEFK + EI L + QI +SITSPV L HLES+LP K+AK+IPSIT Sbjct: 61 SQSDLLFLCIKPLEFKAVIGEIKDRLETRQIAISITSPVQLIHLESSLPCKVAKIIPSIT 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 + G SLC++GSR+ +DR L++ LLS++ P++I E HTRI SDFSSCGPAF+S F+ Sbjct: 121 NITGGGASLCMYGSRIKEEDRQLIESLLSYISRPLKILESHTRITSDFSSCGPAFLSLFM 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 E+WI+AAVE TGID + LA FSP+ELQ+RVAVPGGITA+AL+ Sbjct: 181 EKWIDAAVEMTGIDRRSLNALAGEMLLGTGKLLTEEGFSPEELQKRVAVPGGITAQALAL 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDVEKLDALYG 271 L ++L G+F +LI TTHDKY+ED+ KLDA +G Sbjct: 241 LESNLNGLFYQLIQTTHDKYEEDLAKLDAAFG 272 >B1DFF4.1 B1DFF4_9BACL Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 279 Score = 287 bits (735), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 143/273 (52%), Positives = 195/273 (71%), Gaps = 2/273 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIG-SNQETA 59 M VGFIGTG+MGSLLI+AF++SGAL+PC ++ SNR+ + L +R+PG+ SN TA Sbjct: 1 MNVGFIGTGAMGSLLIEAFVASGALDPCQIVISNRTYAKAQALAERYPGMRAERSNASTA 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 SDI+FICVKPLE+K + DE+ LR EQ+++SITSPV++ HLE LP KIAKVIPS+T Sbjct: 61 SVSDILFICVKPLEYKKVIDELLPVLRPEQLLISITSPVLIGHLEELLPCKIAKVIPSVT 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 + V SG +LCI+GSR+ +D+ L++ +L+F+ P+ I+E +TRI SD SSCGPAF ++ + Sbjct: 121 NLVWSGATLCIYGSRITEEDQTLIEDMLAFISEPLPIEEPYTRIVSDLSSCGPAFFAYLV 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 E++I+AAV TGI E +A SP+++Q+RVAVPGGITA+AL Sbjct: 181 EQFIDAAVCETGISREDAVSVASNMLLGTGLLLTEGGLSPEDIQKRVAVPGGITAQALQL 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDVEKLD-ALYG 271 L L+G F RLI TTH K+DEDV K++ A YG Sbjct: 241 LNRELDGTFNRLIRTTHKKFDEDVAKINTAFYG 273 >C0ZB14.1 C0ZB14_BREBN Putative uncharacterized protein Length = 277 Score = 222 bits (565), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 113/264 (42%), Positives = 167/264 (63%), Gaps = 1/264 (0%) Query: 2 KVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIG-SNQETAQ 60 ++GFIGTGSMGS+LI++ LS+ AL P V+ SNR+P + QL ++HPG+ + SN + A+ Sbjct: 3 RIGFIGTGSMGSILIESLLSAKALTPGHVIISNRTPAKAQQLAEKHPGLIVAHSNADLAK 62 Query: 61 GSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSITH 120 + + +CVKPLE+ + ++I+ +L + ++++ITSP+ L LES +P +A+V+PSIT+ Sbjct: 63 EAITIVLCVKPLEYSVMLEQIAPALTPDHLLITITSPIKLADLESQVPCAVARVVPSITN 122 Query: 121 RVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFLE 180 V SG SLC FGSR+ + R ++ L + + PIEI E RI SD SCGPAFIS+ L+ Sbjct: 123 AVMSGVSLCEFGSRIQEEQRQFIRSLFAHISSPIEISEPFLRITSDIVSCGPAFISYILQ 182 Query: 181 RWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSHL 240 + I+ AVE TGI E T + F+ LQ+RV VPGGIT E L L Sbjct: 183 QMIQEAVEETGISAEAATYMTTQMLIGIADLLREEAFTLPTLQKRVCVPGGITGEGLIPL 242 Query: 241 RTSLEGVFERLILTTHDKYDEDVE 264 + + G+F ++ T K+ ED E Sbjct: 243 QDGIPGLFAQVFRRTQAKFAEDQE 266 >C5D4V6.1 C5D4V6_9BACI Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 273 Score = 216 bits (550), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 109/272 (40%), Positives = 169/272 (62%), Gaps = 1/272 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIG-SNQETA 59 M++G IGTG+MG +LI+AFL SG+++ ++ +NR+ + L +Q+R+PGI + S +E Sbjct: 1 MEIGMIGTGNMGRILIEAFLESGSVKEDQLIITNRTLEKALDIQRRYPGIYVAASAEEVV 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 Q + IVF+CVKPL+ L ++SS E +VSITSP+ + LE+A+P + +VIPSIT Sbjct: 61 QKATIVFLCVKPLDIHPLLQQLSSFWTKEHCLVSITSPISVQQLEAAVPCHVVRVIPSIT 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 +R +G++L G R ++ + ++ L + P I + TRIASD +SCGPAF S+ L Sbjct: 121 NRAFAGSTLITVGQRCSSHYQNYIETLFRQISAPCYINDEITRIASDIASCGPAFFSYLL 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 +R+IEAA T I +E T L ++ L+E+V V GGIT E ++ Sbjct: 181 QRFIEAAAAKTSITKEQATELTSNMIIGLGELLKKNLYTLPTLREKVCVKGGITGEGIAV 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDVEKLDALYG 271 L +EGVFER+ TH+K+ +D+EK+ +G Sbjct: 241 LEKEIEGVFERVFDKTHEKFKDDIEKIKKQFG 272 >A7Z6X4.1 A7Z6X4_BACA2 ComER Length = 273 Score = 213 bits (542), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 106/264 (40%), Positives = 164/264 (62%), Gaps = 1/264 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIGSNQET-A 59 MK+GFIGTG+MG++LI++F+ S A +P + +NR+ + L+L++R GI + +QE Sbjct: 1 MKIGFIGTGNMGTILIESFIESNAADPSHITITNRTIEKALKLKERFSGIHVTEDQEKLV 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 Q ++I+FICVKPL+ L +++ LR + I++SITSPV +E +P + A+VIPSIT Sbjct: 61 QENEIIFICVKPLDIYPLLKKLTPHLRKDHILISITSPVQTDQIEHTVPCQTARVIPSIT 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 +R +G SL FG + + +Q L + + P+EI TR ASD SCGPAF+S+ + Sbjct: 121 NRALAGVSLVTFGKSCSESAKADIQTLFTHISKPVEISSGITRAASDIVSCGPAFMSYLV 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 +R+I+AAVE T I +E ++ ++ LQE+V V GG+T E L Sbjct: 181 QRFIDAAVEETDITKEEAVIMSTEMLVGLGKLLETGHYTLPALQEKVCVKGGVTGEGLKA 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDV 263 L + ++ +F R+ THDKYDED+ Sbjct: 241 LESGVQDMFNRVFQRTHDKYDEDI 264 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,468,831,495 Number of extensions: 52700440 Number of successful extensions: 139789 Number of sequences better than 1.0e-05: 1621 Number of HSP's gapped: 137924 Number of HSP's successfully gapped: 1621 Length of query: 282 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 148 Effective length of database: 1,798,280,193 Effective search space: 266145468564 Effective search space used: 266145468564 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)