BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0018.p (355 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFL0.1 B3KFL0_9BACI Putative uncharacterized protein 509 e-142 B7DV04.1 B7DV04_9BACL Putative uncharacterized protein 256 2e-66 C1PRJ5.1 C1PRJ5_ALIAC Predicted permease 255 5e-66 C0ZAR9.1 C0ZAR9_BREBN Conserved hypothetical membrane protein 249 2e-64 O34472.1 YRRI_BACSU UPF0118 membrane protein yrrI 226 3e-57 >B3KFL0.1 B3KFL0_9BACI Putative uncharacterized protein Length = 355 Score = 509 bits (1312), Expect = e-142, Method: Compositional matrix adjust. Identities = 242/353 (68%), Positives = 298/353 (84%) Query: 1 MEEWSHSKWFRWMIGVLLALIILYFVWLLRPMLQGVFQFLKAILAPFLAAMIISYVLNPV 60 ME+ + +KWFR ++ ++L LI LYFVWLLRP+ V++FLKA+LAPFL AMIISYVLNP+ Sbjct: 1 MEQLTENKWFRLLVWLMLGLISLYFVWLLRPLFLDVYRFLKAVLAPFLVAMIISYVLNPI 60 Query: 61 VSMLAGRKMPRSVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLPEITLHAQGLMR 120 V L+ RK+PRS+AVLLIYAVFLTSLAVILIN+IPM I QLEELNEHLPE++++AQ LM Sbjct: 61 VCTLSDRKVPRSIAVLLIYAVFLTSLAVILINMIPMLIRQLEELNEHLPELSMNAQSLMT 120 Query: 121 NMNTRLIPPGVGMGMNNWFFQLENRLAGGISHFLDNIGSTIGVLFDAFIVPFLVFYILKD 180 N+++RL+PPGV G+N+WF Q+E+RLAGGIS F+DNIG+TI +LF+ IVPFL+FYILKD Sbjct: 121 NLDSRLLPPGVRTGVNSWFLQMESRLAGGISDFMDNIGNTINILFNVLIVPFLIFYILKD 180 Query: 181 FDVFERTVVSCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIIIGVLAYIGYAIIGMPY 240 F+VF+RT VS LPRSRRK IV +L++ID ALGNY+RGQFLVC+IIGVLAY+GY I+GMPY Sbjct: 181 FEVFQRTAVSYLPRSRRKSIVTLLKEIDTALGNYVRGQFLVCLIIGVLAYVGYMIVGMPY 240 Query: 241 ALLFACVVAVFNIVPYMGPFLGAAPAIVMXXXXXXXXXXXXXXXXXXCQMLESNVISPQV 300 ALL A +VAVFNI+PY+GPFLGAAPA+VM CQ LESNVISPQV Sbjct: 241 ALLLASIVAVFNIIPYLGPFLGAAPALVMASTISWRMVLLVAVVNMVCQTLESNVISPQV 300 Query: 301 VGRKLHLHPLLIIFALLVGGEVAGVIGLILAVPVFAAGKVVIQHIMAYYIKRK 353 VG+KLH+HPLLIIFALLVGG+VAG +GLILAVPVFA KV++QH+ AYY+++K Sbjct: 301 VGKKLHMHPLLIIFALLVGGQVAGTVGLILAVPVFAVIKVLLQHVFAYYVRKK 353 >B7DV04.1 B7DV04_9BACL Putative uncharacterized protein Length = 354 Score = 256 bits (655), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 136/340 (40%), Positives = 207/340 (60%), Gaps = 3/340 (0%) Query: 8 KWFRWMIGVLLALIILYFVWLLRPMLQGVFQFLKAILAPFLAAMIISYVLNPVVSMLAGR 67 ++ R M+ + L L +Y V LLR + ++ L ++ FLA++II+Y+L P V +L R Sbjct: 5 RYARTMMSIALTLTCIYLVGLLRSLFLDIWGVLSSLFYTFLASLIITYILQPAVDVLHRR 64 Query: 68 KMPRSVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLPEITLHAQGLMRNMNTR-- 125 ++PRS A+L++YAV LAV+++NLIP+ Q+ +L+ LP IT + +N+R Sbjct: 65 RVPRSAAILIVYAVIAAILAVVVLNLIPVISRQIAQLSSSLPAITAELSRWIDMINSRKT 124 Query: 126 LIPPGVGMGMNNWFFQLENRLAGGISHFLDNIGSTIGVLFDAFIVPFLVFYILKDFDVFE 185 +P V G+ N QLE+R+ + + ST+ +F AF+VPFLVFY+LKD Sbjct: 125 YLPNAVRTGIENALNQLEHRVEVAPTGLFSVVTSTLNAIFMAFVVPFLVFYMLKDAKAIG 184 Query: 186 RTVVSCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIIIGVLAYIGYAIIGMPYALLFA 245 R +V P + + +LR IDD LG Y+RGQFL+ + +G+L++IGY II +PYALL Sbjct: 185 RGMVRMAPAKYKHRVRLVLRAIDDTLGGYVRGQFLIMLAVGLLSFIGYLIIRLPYALLLG 244 Query: 246 CVVAVFNIVPYMGPFLGAAPAIVMXXXXXX-XXXXXXXXXXXXCQMLESNVISPQVVGRK 304 + +I+PY+GP +G APA+++ Q +E N++SPQ++GR Sbjct: 245 AFLGFADIIPYLGPIIGIAPAVIIALTTSGWPMLVKVVIVNIAVQQVEGNILSPQIMGRT 304 Query: 305 LHLHPLLIIFALLVGGEVAGVIGLILAVPVFAAGKVVIQH 344 LHLHP+ I+ AL+VGGE G++GL+LAVP A KVV H Sbjct: 305 LHLHPMAIVAALIVGGEAGGIVGLVLAVPALAVCKVVWMH 344 >C1PRJ5.1 C1PRJ5_ALIAC Predicted permease Length = 352 Score = 255 bits (651), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 136/337 (40%), Positives = 206/337 (61%), Gaps = 3/337 (0%) Query: 8 KWFRWMIGVLLALIILYFVWLLRPMLQGVFQFLKAILAPFLAAMIISYVLNPVVSMLAGR 67 ++ R M+ + L L +Y V LLR + ++ L ++ FLA++II+Y+L P V +L R Sbjct: 3 RYARTMMSIALTLTCIYLVGLLRSLFLDIWGVLSSLFYTFLASLIITYILQPAVDVLHRR 62 Query: 68 KMPRSVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLPEITLHAQGLMRNMNTR-- 125 ++PRS A+L++YAV LAV+++NLIP+ Q+ +L+ LP IT + +N+R Sbjct: 63 RVPRSAAILIVYAVIAAILAVVVLNLIPVISRQIAQLSSSLPAITAELSRWIDMINSRKT 122 Query: 126 LIPPGVGMGMNNWFFQLENRLAGGISHFLDNIGSTIGVLFDAFIVPFLVFYILKDFDVFE 185 +P V G+ N QLE+R+ + + ST+ +F AF+VPFLVFY+LKD Sbjct: 123 YLPNAVRTGIENALNQLEHRVEVAPTGLFSVVTSTLNAIFMAFVVPFLVFYMLKDAKAIG 182 Query: 186 RTVVSCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIIIGVLAYIGYAIIGMPYALLFA 245 R +V P + + +LR IDD LG Y+RGQFL+ + +G+L++IGY II +PYALL Sbjct: 183 RGMVRMAPAKYKHRVRLVLRAIDDTLGGYVRGQFLIMLAVGLLSFIGYLIIRLPYALLLG 242 Query: 246 CVVAVFNIVPYMGPFLGAAPAIVMXXXXXX-XXXXXXXXXXXXCQMLESNVISPQVVGRK 304 + +I+PY+GP +G APA+++ Q +E N++SPQ++GR Sbjct: 243 AFLGFADIIPYLGPIIGIAPAVIIALTTSGWPMLVKVVIVNIAVQQIEGNILSPQIMGRT 302 Query: 305 LHLHPLLIIFALLVGGEVAGVIGLILAVPVFAAGKVV 341 LHLHP+ I+ AL+VGGE G++GLILAVP A KVV Sbjct: 303 LHLHPMAIVAALIVGGEAGGILGLILAVPALAVCKVV 339 >C0ZAR9.1 C0ZAR9_BREBN Conserved hypothetical membrane protein Length = 354 Score = 249 bits (637), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 140/347 (40%), Positives = 213/347 (61%), Gaps = 2/347 (0%) Query: 1 MEEWSHSKWFRWMIGVLLALIILYFVWLLRPMLQGVFQFLKAILAPFLAAMIISYVLNPV 60 M+E S+ F + V+ L+I +WLLRP+L +F +K +L P + ++I+Y+L+P+ Sbjct: 1 MDELRRSRLFAAAVWVITLLVIGNLLWLLRPVLSQLFSLIKEVLVPVILGLVIAYLLHPI 60 Query: 61 VSMLAGRKMPRSVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLPEITLHAQGLMR 120 V +L R++PR +AVLLIY F+ + + +IN IP+F +QL EL++ +P + M Sbjct: 61 VQLLEKRRVPRLMAVLLIYGSFVLVITIAIINAIPVFTKQLVELSDDIPRLMDWYYTWMS 120 Query: 121 NMNTR--LIPPGVGMGMNNWFFQLENRLAGGISHFLDNIGSTIGVLFDAFIVPFLVFYIL 178 R +P + G++ Q ++ +S +DN ++G LF IVPF+ FY L Sbjct: 121 EWEARKYFLPDSISKGVDRVIIQSNEGMSHSVSKIVDNAKHSMGKLFAFAIVPFIAFYFL 180 Query: 179 KDFDVFERTVVSCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIIIGVLAYIGYAIIGM 238 KD T +S +P++ RK ++ +LRDI+++LG YI GQ +V +I+GV AY+GY IGM Sbjct: 181 KDMKQLHETGMSIVPKAYRKQVLIVLRDINESLGKYIHGQMMVALIVGVFAYLGYWWIGM 240 Query: 239 PYALLFACVVAVFNIVPYMGPFLGAAPAIVMXXXXXXXXXXXXXXXXXXCQMLESNVISP 298 PY + A V + NI+PY+GP +GAAPA+V+ Q++E N++SP Sbjct: 241 PYPFVLAAFVCLTNIIPYIGPLIGAAPAVVIAITISTKTLLLVVVVNLIIQIVEGNILSP 300 Query: 299 QVVGRKLHLHPLLIIFALLVGGEVAGVIGLILAVPVFAAGKVVIQHI 345 +VGR LHLHPLLII ALLVG V G+IGLI+AVP+ A KV++ I Sbjct: 301 NIVGRSLHLHPLLIILALLVGETVGGIIGLIVAVPILAVCKVIVSRI 347 >O34472.1 YRRI_BACSU UPF0118 membrane protein yrrI Length = 353 Score = 226 bits (576), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 122/337 (36%), Positives = 199/337 (59%), Gaps = 2/337 (0%) Query: 12 WMIGVLLALIILYFVWLLRPMLQGVFQFLKAILAPFLAAMIISYVLNPVVSMLAGRKMPR 71 W+ LL L+ +Y ++L + + +K I P + ++ ISY+L PV L G+ +PR Sbjct: 11 WVAICLLVLLTVYVFFMLDMLWSPFWLVIKTIFIPLIISIFISYLLLPVTEWLHGKGLPR 70 Query: 72 SVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLPEITLHAQGLMRNMN--TRLIPP 129 ++++L+IY +F + L +P+ I QL +L+E++P GL+ +++ T P Sbjct: 71 TLSILVIYVLFFGGIGWALYKGVPVLIVQLTDLSENIPMFAETYNGLLLHVHNHTDDWPD 130 Query: 130 GVGMGMNNWFFQLENRLAGGISHFLDNIGSTIGVLFDAFIVPFLVFYILKDFDVFERTVV 189 G+ ++ Q E AG I + I + + A +PFLVFY++KD ++ ++TV Sbjct: 131 GMHHRIDKMIRQTEAFFAGTIEGAISGIRNVLDYFLIAATIPFLVFYMVKDIELMKKTVW 190 Query: 190 SCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIIIGVLAYIGYAIIGMPYALLFACVVA 249 P+S RK LRD+DD+LG+YIRGQ LVC+I+GV+A I + + G+PY L+ + Sbjct: 191 YLTPKSWRKRGSAFLRDVDDSLGDYIRGQLLVCLILGVIAGISFWVFGLPYPLILGLISG 250 Query: 250 VFNIVPYMGPFLGAAPAIVMXXXXXXXXXXXXXXXXXXCQMLESNVISPQVVGRKLHLHP 309 V N++PY GPF+GA PA+++ Q +E N++SP +VGR L +HP Sbjct: 251 VTNVIPYFGPFIGAVPALLIAMTISVKAVLVVVITVFILQFMEGNILSPFIVGRSLKMHP 310 Query: 310 LLIIFALLVGGEVAGVIGLILAVPVFAAGKVVIQHIM 346 ++I+ ALL GGE+AG++G+ILAVP A KV++ H + Sbjct: 311 VVIMLALLAGGELAGIVGMILAVPATAVLKVMMIHFL 347 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.333 0.148 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,994,193,310 Number of extensions: 79720748 Number of successful extensions: 340574 Number of sequences better than 1.0e-05: 3245 Number of HSP's gapped: 334744 Number of HSP's successfully gapped: 3247 Length of query: 355 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 218 Effective length of database: 1,770,017,148 Effective search space: 385863738264 Effective search space used: 385863738264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 132 (55.5 bits)